Job ID = 6368391 SRX = SRX495070 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:20:21 prefetch.2.10.7: 1) Downloading 'SRR1198602'... 2020-06-16T00:20:21 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:27:30 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:27:30 prefetch.2.10.7: 1) 'SRR1198602' was downloaded successfully Read 47950615 spots for SRR1198602/SRR1198602.sra Written 47950615 spots for SRR1198602/SRR1198602.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:37 47950615 reads; of these: 47950615 (100.00%) were unpaired; of these: 3138648 (6.55%) aligned 0 times 37486073 (78.18%) aligned exactly 1 time 7325894 (15.28%) aligned >1 times 93.45% overall alignment rate Time searching: 00:10:37 Overall time: 00:10:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8189376 / 44811967 = 0.1827 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:50:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX495070/SRX495070.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX495070/SRX495070.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX495070/SRX495070.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX495070/SRX495070.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:50:44: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:50:44: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:50:51: 1000000 INFO @ Tue, 16 Jun 2020 09:50:57: 2000000 INFO @ Tue, 16 Jun 2020 09:51:04: 3000000 INFO @ Tue, 16 Jun 2020 09:51:10: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:51:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX495070/SRX495070.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX495070/SRX495070.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX495070/SRX495070.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX495070/SRX495070.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:51:14: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:51:14: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:51:17: 5000000 INFO @ Tue, 16 Jun 2020 09:51:21: 1000000 INFO @ Tue, 16 Jun 2020 09:51:24: 6000000 INFO @ Tue, 16 Jun 2020 09:51:28: 2000000 INFO @ Tue, 16 Jun 2020 09:51:31: 7000000 INFO @ Tue, 16 Jun 2020 09:51:34: 3000000 INFO @ Tue, 16 Jun 2020 09:51:38: 8000000 INFO @ Tue, 16 Jun 2020 09:51:41: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:51:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX495070/SRX495070.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX495070/SRX495070.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX495070/SRX495070.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX495070/SRX495070.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:51:44: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:51:44: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:51:45: 9000000 INFO @ Tue, 16 Jun 2020 09:51:48: 5000000 INFO @ Tue, 16 Jun 2020 09:51:51: 1000000 INFO @ Tue, 16 Jun 2020 09:51:53: 10000000 INFO @ Tue, 16 Jun 2020 09:51:55: 6000000 INFO @ Tue, 16 Jun 2020 09:51:58: 2000000 INFO @ Tue, 16 Jun 2020 09:52:00: 11000000 INFO @ Tue, 16 Jun 2020 09:52:02: 7000000 INFO @ Tue, 16 Jun 2020 09:52:05: 3000000 INFO @ Tue, 16 Jun 2020 09:52:08: 12000000 INFO @ Tue, 16 Jun 2020 09:52:09: 8000000 INFO @ Tue, 16 Jun 2020 09:52:12: 4000000 INFO @ Tue, 16 Jun 2020 09:52:15: 13000000 INFO @ Tue, 16 Jun 2020 09:52:16: 9000000 INFO @ Tue, 16 Jun 2020 09:52:20: 5000000 INFO @ Tue, 16 Jun 2020 09:52:23: 14000000 INFO @ Tue, 16 Jun 2020 09:52:23: 10000000 INFO @ Tue, 16 Jun 2020 09:52:27: 6000000 INFO @ Tue, 16 Jun 2020 09:52:30: 15000000 INFO @ Tue, 16 Jun 2020 09:52:31: 11000000 INFO @ Tue, 16 Jun 2020 09:52:34: 7000000 INFO @ Tue, 16 Jun 2020 09:52:38: 12000000 INFO @ Tue, 16 Jun 2020 09:52:38: 16000000 INFO @ Tue, 16 Jun 2020 09:52:42: 8000000 INFO @ Tue, 16 Jun 2020 09:52:45: 13000000 INFO @ Tue, 16 Jun 2020 09:52:46: 17000000 INFO @ Tue, 16 Jun 2020 09:52:49: 9000000 INFO @ Tue, 16 Jun 2020 09:52:52: 14000000 INFO @ Tue, 16 Jun 2020 09:52:54: 18000000 INFO @ Tue, 16 Jun 2020 09:52:57: 10000000 INFO @ Tue, 16 Jun 2020 09:53:00: 15000000 INFO @ Tue, 16 Jun 2020 09:53:02: 19000000 INFO @ Tue, 16 Jun 2020 09:53:04: 11000000 INFO @ Tue, 16 Jun 2020 09:53:07: 16000000 INFO @ Tue, 16 Jun 2020 09:53:11: 20000000 INFO @ Tue, 16 Jun 2020 09:53:11: 12000000 INFO @ Tue, 16 Jun 2020 09:53:14: 17000000 INFO @ Tue, 16 Jun 2020 09:53:19: 13000000 INFO @ Tue, 16 Jun 2020 09:53:19: 21000000 INFO @ Tue, 16 Jun 2020 09:53:22: 18000000 INFO @ Tue, 16 Jun 2020 09:53:26: 14000000 INFO @ Tue, 16 Jun 2020 09:53:27: 22000000 INFO @ Tue, 16 Jun 2020 09:53:29: 19000000 INFO @ Tue, 16 Jun 2020 09:53:33: 15000000 INFO @ Tue, 16 Jun 2020 09:53:34: 23000000 INFO @ Tue, 16 Jun 2020 09:53:36: 20000000 INFO @ Tue, 16 Jun 2020 09:53:40: 16000000 INFO @ Tue, 16 Jun 2020 09:53:41: 24000000 INFO @ Tue, 16 Jun 2020 09:53:43: 21000000 INFO @ Tue, 16 Jun 2020 09:53:48: 25000000 INFO @ Tue, 16 Jun 2020 09:53:48: 17000000 INFO @ Tue, 16 Jun 2020 09:53:51: 22000000 INFO @ Tue, 16 Jun 2020 09:53:54: 26000000 INFO @ Tue, 16 Jun 2020 09:53:55: 18000000 INFO @ Tue, 16 Jun 2020 09:53:58: 23000000 INFO @ Tue, 16 Jun 2020 09:54:01: 27000000 INFO @ Tue, 16 Jun 2020 09:54:02: 19000000 INFO @ Tue, 16 Jun 2020 09:54:06: 24000000 INFO @ Tue, 16 Jun 2020 09:54:07: 28000000 INFO @ Tue, 16 Jun 2020 09:54:08: 20000000 INFO @ Tue, 16 Jun 2020 09:54:13: 25000000 INFO @ Tue, 16 Jun 2020 09:54:14: 29000000 INFO @ Tue, 16 Jun 2020 09:54:15: 21000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:54:21: 26000000 INFO @ Tue, 16 Jun 2020 09:54:21: 30000000 INFO @ Tue, 16 Jun 2020 09:54:23: 22000000 INFO @ Tue, 16 Jun 2020 09:54:27: 31000000 INFO @ Tue, 16 Jun 2020 09:54:28: 27000000 INFO @ Tue, 16 Jun 2020 09:54:30: 23000000 INFO @ Tue, 16 Jun 2020 09:54:34: 32000000 INFO @ Tue, 16 Jun 2020 09:54:35: 28000000 INFO @ Tue, 16 Jun 2020 09:54:38: 24000000 INFO @ Tue, 16 Jun 2020 09:54:40: 33000000 INFO @ Tue, 16 Jun 2020 09:54:43: 29000000 INFO @ Tue, 16 Jun 2020 09:54:45: 25000000 INFO @ Tue, 16 Jun 2020 09:54:46: 34000000 INFO @ Tue, 16 Jun 2020 09:54:51: 30000000 INFO @ Tue, 16 Jun 2020 09:54:52: 26000000 INFO @ Tue, 16 Jun 2020 09:54:54: 35000000 INFO @ Tue, 16 Jun 2020 09:54:58: 31000000 INFO @ Tue, 16 Jun 2020 09:54:59: 27000000 INFO @ Tue, 16 Jun 2020 09:55:01: 36000000 INFO @ Tue, 16 Jun 2020 09:55:05: 32000000 INFO @ Tue, 16 Jun 2020 09:55:06: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:55:06: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:55:06: #1 total tags in treatment: 36622591 INFO @ Tue, 16 Jun 2020 09:55:06: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:55:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:55:06: 28000000 INFO @ Tue, 16 Jun 2020 09:55:06: #1 tags after filtering in treatment: 36622591 INFO @ Tue, 16 Jun 2020 09:55:06: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:55:06: #1 finished! INFO @ Tue, 16 Jun 2020 09:55:06: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:55:06: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:55:09: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:55:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:55:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX495070/SRX495070.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX495070/SRX495070.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX495070/SRX495070.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX495070/SRX495070.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:55:13: 33000000 INFO @ Tue, 16 Jun 2020 09:55:13: 29000000 INFO @ Tue, 16 Jun 2020 09:55:20: 34000000 INFO @ Tue, 16 Jun 2020 09:55:20: 30000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 09:55:26: 35000000 INFO @ Tue, 16 Jun 2020 09:55:27: 31000000 INFO @ Tue, 16 Jun 2020 09:55:33: 36000000 INFO @ Tue, 16 Jun 2020 09:55:33: 32000000 INFO @ Tue, 16 Jun 2020 09:55:37: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:55:37: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:55:37: #1 total tags in treatment: 36622591 INFO @ Tue, 16 Jun 2020 09:55:37: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:55:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:55:38: #1 tags after filtering in treatment: 36622591 INFO @ Tue, 16 Jun 2020 09:55:38: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:55:38: #1 finished! INFO @ Tue, 16 Jun 2020 09:55:38: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:55:38: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:55:40: 33000000 INFO @ Tue, 16 Jun 2020 09:55:40: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:55:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:55:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX495070/SRX495070.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX495070/SRX495070.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX495070/SRX495070.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX495070/SRX495070.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:55:46: 34000000 INFO @ Tue, 16 Jun 2020 09:55:52: 35000000 INFO @ Tue, 16 Jun 2020 09:55:59: 36000000 INFO @ Tue, 16 Jun 2020 09:56:03: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:56:03: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:56:03: #1 total tags in treatment: 36622591 INFO @ Tue, 16 Jun 2020 09:56:03: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:56:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:56:04: #1 tags after filtering in treatment: 36622591 INFO @ Tue, 16 Jun 2020 09:56:04: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:56:04: #1 finished! INFO @ Tue, 16 Jun 2020 09:56:04: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:56:04: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:56:06: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:56:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:56:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX495070/SRX495070.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX495070/SRX495070.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX495070/SRX495070.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX495070/SRX495070.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling