Job ID = 6368207 SRX = SRX494890 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:12:35 prefetch.2.10.7: 1) Downloading 'SRR1198422'... 2020-06-16T00:12:35 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:19:23 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:19:23 prefetch.2.10.7: 1) 'SRR1198422' was downloaded successfully Read 47950615 spots for SRR1198422/SRR1198422.sra Written 47950615 spots for SRR1198422/SRR1198422.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:35 47950615 reads; of these: 47950615 (100.00%) were unpaired; of these: 3138680 (6.55%) aligned 0 times 37485903 (78.18%) aligned exactly 1 time 7326032 (15.28%) aligned >1 times 93.45% overall alignment rate Time searching: 00:10:35 Overall time: 00:10:35 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8188849 / 44811935 = 0.1827 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:42:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX494890/SRX494890.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX494890/SRX494890.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX494890/SRX494890.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX494890/SRX494890.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:42:53: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:42:53: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:43:00: 1000000 INFO @ Tue, 16 Jun 2020 09:43:07: 2000000 INFO @ Tue, 16 Jun 2020 09:43:14: 3000000 INFO @ Tue, 16 Jun 2020 09:43:20: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:43:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX494890/SRX494890.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX494890/SRX494890.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX494890/SRX494890.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX494890/SRX494890.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:43:23: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:43:23: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:43:27: 5000000 INFO @ Tue, 16 Jun 2020 09:43:30: 1000000 INFO @ Tue, 16 Jun 2020 09:43:35: 6000000 INFO @ Tue, 16 Jun 2020 09:43:37: 2000000 INFO @ Tue, 16 Jun 2020 09:43:42: 7000000 INFO @ Tue, 16 Jun 2020 09:43:43: 3000000 INFO @ Tue, 16 Jun 2020 09:43:49: 8000000 INFO @ Tue, 16 Jun 2020 09:43:50: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:43:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX494890/SRX494890.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX494890/SRX494890.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX494890/SRX494890.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX494890/SRX494890.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:43:53: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:43:53: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:43:56: 9000000 INFO @ Tue, 16 Jun 2020 09:43:56: 5000000 INFO @ Tue, 16 Jun 2020 09:44:00: 1000000 INFO @ Tue, 16 Jun 2020 09:44:03: 6000000 INFO @ Tue, 16 Jun 2020 09:44:03: 10000000 INFO @ Tue, 16 Jun 2020 09:44:07: 2000000 INFO @ Tue, 16 Jun 2020 09:44:10: 7000000 INFO @ Tue, 16 Jun 2020 09:44:11: 11000000 INFO @ Tue, 16 Jun 2020 09:44:13: 3000000 INFO @ Tue, 16 Jun 2020 09:44:16: 8000000 INFO @ Tue, 16 Jun 2020 09:44:18: 12000000 INFO @ Tue, 16 Jun 2020 09:44:20: 4000000 INFO @ Tue, 16 Jun 2020 09:44:23: 9000000 INFO @ Tue, 16 Jun 2020 09:44:25: 13000000 INFO @ Tue, 16 Jun 2020 09:44:26: 5000000 INFO @ Tue, 16 Jun 2020 09:44:29: 10000000 INFO @ Tue, 16 Jun 2020 09:44:32: 14000000 INFO @ Tue, 16 Jun 2020 09:44:33: 6000000 INFO @ Tue, 16 Jun 2020 09:44:36: 11000000 INFO @ Tue, 16 Jun 2020 09:44:38: 15000000 INFO @ Tue, 16 Jun 2020 09:44:39: 7000000 INFO @ Tue, 16 Jun 2020 09:44:43: 12000000 INFO @ Tue, 16 Jun 2020 09:44:45: 16000000 INFO @ Tue, 16 Jun 2020 09:44:46: 8000000 INFO @ Tue, 16 Jun 2020 09:44:49: 13000000 INFO @ Tue, 16 Jun 2020 09:44:52: 17000000 INFO @ Tue, 16 Jun 2020 09:44:53: 9000000 INFO @ Tue, 16 Jun 2020 09:44:56: 14000000 INFO @ Tue, 16 Jun 2020 09:44:59: 10000000 INFO @ Tue, 16 Jun 2020 09:44:59: 18000000 INFO @ Tue, 16 Jun 2020 09:45:02: 15000000 INFO @ Tue, 16 Jun 2020 09:45:06: 11000000 INFO @ Tue, 16 Jun 2020 09:45:06: 19000000 INFO @ Tue, 16 Jun 2020 09:45:09: 16000000 INFO @ Tue, 16 Jun 2020 09:45:12: 12000000 INFO @ Tue, 16 Jun 2020 09:45:14: 20000000 INFO @ Tue, 16 Jun 2020 09:45:16: 17000000 INFO @ Tue, 16 Jun 2020 09:45:19: 13000000 INFO @ Tue, 16 Jun 2020 09:45:21: 21000000 INFO @ Tue, 16 Jun 2020 09:45:22: 18000000 INFO @ Tue, 16 Jun 2020 09:45:25: 14000000 INFO @ Tue, 16 Jun 2020 09:45:28: 22000000 INFO @ Tue, 16 Jun 2020 09:45:29: 19000000 INFO @ Tue, 16 Jun 2020 09:45:32: 15000000 INFO @ Tue, 16 Jun 2020 09:45:35: 23000000 INFO @ Tue, 16 Jun 2020 09:45:36: 20000000 INFO @ Tue, 16 Jun 2020 09:45:38: 16000000 INFO @ Tue, 16 Jun 2020 09:45:42: 24000000 INFO @ Tue, 16 Jun 2020 09:45:43: 21000000 INFO @ Tue, 16 Jun 2020 09:45:45: 17000000 INFO @ Tue, 16 Jun 2020 09:45:49: 25000000 INFO @ Tue, 16 Jun 2020 09:45:49: 22000000 INFO @ Tue, 16 Jun 2020 09:45:51: 18000000 INFO @ Tue, 16 Jun 2020 09:45:56: 23000000 INFO @ Tue, 16 Jun 2020 09:45:57: 26000000 INFO @ Tue, 16 Jun 2020 09:45:58: 19000000 INFO @ Tue, 16 Jun 2020 09:46:03: 24000000 INFO @ Tue, 16 Jun 2020 09:46:04: 27000000 INFO @ Tue, 16 Jun 2020 09:46:04: 20000000 INFO @ Tue, 16 Jun 2020 09:46:09: 25000000 INFO @ Tue, 16 Jun 2020 09:46:11: 21000000 INFO @ Tue, 16 Jun 2020 09:46:11: 28000000 INFO @ Tue, 16 Jun 2020 09:46:16: 26000000 INFO @ Tue, 16 Jun 2020 09:46:17: 22000000 INFO @ Tue, 16 Jun 2020 09:46:18: 29000000 INFO @ Tue, 16 Jun 2020 09:46:23: 27000000 INFO @ Tue, 16 Jun 2020 09:46:24: 23000000 INFO @ Tue, 16 Jun 2020 09:46:25: 30000000 INFO @ Tue, 16 Jun 2020 09:46:30: 28000000 INFO @ Tue, 16 Jun 2020 09:46:30: 24000000 INFO @ Tue, 16 Jun 2020 09:46:33: 31000000 INFO @ Tue, 16 Jun 2020 09:46:36: 29000000 INFO @ Tue, 16 Jun 2020 09:46:37: 25000000 INFO @ Tue, 16 Jun 2020 09:46:40: 32000000 INFO @ Tue, 16 Jun 2020 09:46:43: 30000000 INFO @ Tue, 16 Jun 2020 09:46:44: 26000000 INFO @ Tue, 16 Jun 2020 09:46:47: 33000000 INFO @ Tue, 16 Jun 2020 09:46:50: 31000000 INFO @ Tue, 16 Jun 2020 09:46:51: 27000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:46:54: 34000000 INFO @ Tue, 16 Jun 2020 09:46:57: 32000000 INFO @ Tue, 16 Jun 2020 09:46:57: 28000000 INFO @ Tue, 16 Jun 2020 09:47:02: 35000000 INFO @ Tue, 16 Jun 2020 09:47:03: 33000000 INFO @ Tue, 16 Jun 2020 09:47:04: 29000000 INFO @ Tue, 16 Jun 2020 09:47:09: 36000000 INFO @ Tue, 16 Jun 2020 09:47:10: 34000000 INFO @ Tue, 16 Jun 2020 09:47:11: 30000000 INFO @ Tue, 16 Jun 2020 09:47:13: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:47:13: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:47:13: #1 total tags in treatment: 36623086 INFO @ Tue, 16 Jun 2020 09:47:13: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:47:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:47:14: #1 tags after filtering in treatment: 36623086 INFO @ Tue, 16 Jun 2020 09:47:14: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:47:14: #1 finished! INFO @ Tue, 16 Jun 2020 09:47:14: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:47:14: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:47:16: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:47:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:47:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX494890/SRX494890.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494890/SRX494890.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494890/SRX494890.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494890/SRX494890.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:47:17: 35000000 INFO @ Tue, 16 Jun 2020 09:47:17: 31000000 INFO @ Tue, 16 Jun 2020 09:47:23: 32000000 INFO @ Tue, 16 Jun 2020 09:47:23: 36000000 INFO @ Tue, 16 Jun 2020 09:47:27: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:47:27: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:47:27: #1 total tags in treatment: 36623086 INFO @ Tue, 16 Jun 2020 09:47:27: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:47:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:47:28: #1 tags after filtering in treatment: 36623086 INFO @ Tue, 16 Jun 2020 09:47:28: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:47:28: #1 finished! INFO @ Tue, 16 Jun 2020 09:47:28: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:47:28: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:47:29: 33000000 INFO @ Tue, 16 Jun 2020 09:47:30: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:47:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:47:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX494890/SRX494890.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494890/SRX494890.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494890/SRX494890.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494890/SRX494890.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:47:35: 34000000 INFO @ Tue, 16 Jun 2020 09:47:40: 35000000 INFO @ Tue, 16 Jun 2020 09:47:46: 36000000 INFO @ Tue, 16 Jun 2020 09:47:50: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:47:50: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:47:50: #1 total tags in treatment: 36623086 INFO @ Tue, 16 Jun 2020 09:47:50: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:47:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:47:50: #1 tags after filtering in treatment: 36623086 INFO @ Tue, 16 Jun 2020 09:47:50: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:47:50: #1 finished! INFO @ Tue, 16 Jun 2020 09:47:50: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:47:50: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:47:52: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:47:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:47:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX494890/SRX494890.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494890/SRX494890.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494890/SRX494890.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494890/SRX494890.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。