Job ID = 6368200 SRX = SRX494883 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:13:35 prefetch.2.10.7: 1) Downloading 'SRR1198415'... 2020-06-16T00:13:35 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:20:30 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:20:30 prefetch.2.10.7: 1) 'SRR1198415' was downloaded successfully Read 47950615 spots for SRR1198415/SRR1198415.sra Written 47950615 spots for SRR1198415/SRR1198415.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:43 47950615 reads; of these: 47950615 (100.00%) were unpaired; of these: 3138717 (6.55%) aligned 0 times 37486035 (78.18%) aligned exactly 1 time 7325863 (15.28%) aligned >1 times 93.45% overall alignment rate Time searching: 00:10:43 Overall time: 00:10:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8188734 / 44811898 = 0.1827 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:43:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX494883/SRX494883.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX494883/SRX494883.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX494883/SRX494883.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX494883/SRX494883.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:43:56: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:43:56: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:44:03: 1000000 INFO @ Tue, 16 Jun 2020 09:44:10: 2000000 INFO @ Tue, 16 Jun 2020 09:44:17: 3000000 INFO @ Tue, 16 Jun 2020 09:44:24: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:44:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX494883/SRX494883.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX494883/SRX494883.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX494883/SRX494883.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX494883/SRX494883.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:44:26: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:44:26: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:44:31: 5000000 INFO @ Tue, 16 Jun 2020 09:44:33: 1000000 INFO @ Tue, 16 Jun 2020 09:44:39: 6000000 INFO @ Tue, 16 Jun 2020 09:44:41: 2000000 INFO @ Tue, 16 Jun 2020 09:44:47: 7000000 INFO @ Tue, 16 Jun 2020 09:44:48: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:44:54: 8000000 INFO @ Tue, 16 Jun 2020 09:44:55: 4000000 INFO @ Tue, 16 Jun 2020 09:44:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX494883/SRX494883.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX494883/SRX494883.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX494883/SRX494883.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX494883/SRX494883.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:44:56: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:44:56: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:45:02: 9000000 INFO @ Tue, 16 Jun 2020 09:45:03: 5000000 INFO @ Tue, 16 Jun 2020 09:45:04: 1000000 INFO @ Tue, 16 Jun 2020 09:45:10: 10000000 INFO @ Tue, 16 Jun 2020 09:45:11: 6000000 INFO @ Tue, 16 Jun 2020 09:45:11: 2000000 INFO @ Tue, 16 Jun 2020 09:45:18: 11000000 INFO @ Tue, 16 Jun 2020 09:45:18: 7000000 INFO @ Tue, 16 Jun 2020 09:45:19: 3000000 INFO @ Tue, 16 Jun 2020 09:45:25: 12000000 INFO @ Tue, 16 Jun 2020 09:45:26: 4000000 INFO @ Tue, 16 Jun 2020 09:45:26: 8000000 INFO @ Tue, 16 Jun 2020 09:45:33: 13000000 INFO @ Tue, 16 Jun 2020 09:45:33: 5000000 INFO @ Tue, 16 Jun 2020 09:45:34: 9000000 INFO @ Tue, 16 Jun 2020 09:45:41: 14000000 INFO @ Tue, 16 Jun 2020 09:45:41: 6000000 INFO @ Tue, 16 Jun 2020 09:45:41: 10000000 INFO @ Tue, 16 Jun 2020 09:45:48: 15000000 INFO @ Tue, 16 Jun 2020 09:45:48: 7000000 INFO @ Tue, 16 Jun 2020 09:45:49: 11000000 INFO @ Tue, 16 Jun 2020 09:45:55: 16000000 INFO @ Tue, 16 Jun 2020 09:45:56: 8000000 INFO @ Tue, 16 Jun 2020 09:45:57: 12000000 INFO @ Tue, 16 Jun 2020 09:46:03: 17000000 INFO @ Tue, 16 Jun 2020 09:46:03: 9000000 INFO @ Tue, 16 Jun 2020 09:46:04: 13000000 INFO @ Tue, 16 Jun 2020 09:46:10: 18000000 INFO @ Tue, 16 Jun 2020 09:46:11: 10000000 INFO @ Tue, 16 Jun 2020 09:46:12: 14000000 INFO @ Tue, 16 Jun 2020 09:46:18: 19000000 INFO @ Tue, 16 Jun 2020 09:46:18: 11000000 INFO @ Tue, 16 Jun 2020 09:46:19: 15000000 INFO @ Tue, 16 Jun 2020 09:46:25: 20000000 INFO @ Tue, 16 Jun 2020 09:46:26: 16000000 INFO @ Tue, 16 Jun 2020 09:46:26: 12000000 INFO @ Tue, 16 Jun 2020 09:46:32: 21000000 INFO @ Tue, 16 Jun 2020 09:46:33: 17000000 INFO @ Tue, 16 Jun 2020 09:46:33: 13000000 INFO @ Tue, 16 Jun 2020 09:46:39: 22000000 INFO @ Tue, 16 Jun 2020 09:46:40: 18000000 INFO @ Tue, 16 Jun 2020 09:46:41: 14000000 INFO @ Tue, 16 Jun 2020 09:46:47: 23000000 INFO @ Tue, 16 Jun 2020 09:46:47: 19000000 INFO @ Tue, 16 Jun 2020 09:46:48: 15000000 INFO @ Tue, 16 Jun 2020 09:46:54: 20000000 INFO @ Tue, 16 Jun 2020 09:46:54: 24000000 INFO @ Tue, 16 Jun 2020 09:46:56: 16000000 INFO @ Tue, 16 Jun 2020 09:47:01: 21000000 INFO @ Tue, 16 Jun 2020 09:47:01: 25000000 INFO @ Tue, 16 Jun 2020 09:47:03: 17000000 INFO @ Tue, 16 Jun 2020 09:47:08: 22000000 INFO @ Tue, 16 Jun 2020 09:47:08: 26000000 INFO @ Tue, 16 Jun 2020 09:47:10: 18000000 INFO @ Tue, 16 Jun 2020 09:47:15: 23000000 INFO @ Tue, 16 Jun 2020 09:47:15: 27000000 INFO @ Tue, 16 Jun 2020 09:47:17: 19000000 INFO @ Tue, 16 Jun 2020 09:47:22: 24000000 INFO @ Tue, 16 Jun 2020 09:47:23: 28000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:47:24: 20000000 INFO @ Tue, 16 Jun 2020 09:47:29: 25000000 INFO @ Tue, 16 Jun 2020 09:47:30: 29000000 INFO @ Tue, 16 Jun 2020 09:47:31: 21000000 INFO @ Tue, 16 Jun 2020 09:47:36: 26000000 INFO @ Tue, 16 Jun 2020 09:47:37: 30000000 INFO @ Tue, 16 Jun 2020 09:47:39: 22000000 INFO @ Tue, 16 Jun 2020 09:47:44: 27000000 INFO @ Tue, 16 Jun 2020 09:47:44: 31000000 INFO @ Tue, 16 Jun 2020 09:47:46: 23000000 INFO @ Tue, 16 Jun 2020 09:47:51: 28000000 INFO @ Tue, 16 Jun 2020 09:47:52: 32000000 INFO @ Tue, 16 Jun 2020 09:47:53: 24000000 INFO @ Tue, 16 Jun 2020 09:47:58: 29000000 INFO @ Tue, 16 Jun 2020 09:47:59: 33000000 INFO @ Tue, 16 Jun 2020 09:48:00: 25000000 INFO @ Tue, 16 Jun 2020 09:48:05: 30000000 INFO @ Tue, 16 Jun 2020 09:48:06: 34000000 INFO @ Tue, 16 Jun 2020 09:48:07: 26000000 INFO @ Tue, 16 Jun 2020 09:48:12: 31000000 INFO @ Tue, 16 Jun 2020 09:48:13: 35000000 INFO @ Tue, 16 Jun 2020 09:48:14: 27000000 INFO @ Tue, 16 Jun 2020 09:48:19: 32000000 INFO @ Tue, 16 Jun 2020 09:48:20: 36000000 INFO @ Tue, 16 Jun 2020 09:48:21: 28000000 INFO @ Tue, 16 Jun 2020 09:48:25: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:48:25: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:48:25: #1 total tags in treatment: 36623164 INFO @ Tue, 16 Jun 2020 09:48:25: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:48:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:48:26: #1 tags after filtering in treatment: 36623164 INFO @ Tue, 16 Jun 2020 09:48:26: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:48:26: #1 finished! INFO @ Tue, 16 Jun 2020 09:48:26: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:48:26: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:48:26: 33000000 INFO @ Tue, 16 Jun 2020 09:48:28: 29000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 09:48:28: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:48:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:48:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX494883/SRX494883.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494883/SRX494883.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494883/SRX494883.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494883/SRX494883.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:48:34: 34000000 INFO @ Tue, 16 Jun 2020 09:48:35: 30000000 INFO @ Tue, 16 Jun 2020 09:48:41: 35000000 INFO @ Tue, 16 Jun 2020 09:48:42: 31000000 INFO @ Tue, 16 Jun 2020 09:48:48: 36000000 INFO @ Tue, 16 Jun 2020 09:48:49: 32000000 INFO @ Tue, 16 Jun 2020 09:48:53: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:48:53: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:48:53: #1 total tags in treatment: 36623164 INFO @ Tue, 16 Jun 2020 09:48:53: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:48:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:48:53: #1 tags after filtering in treatment: 36623164 INFO @ Tue, 16 Jun 2020 09:48:53: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:48:53: #1 finished! INFO @ Tue, 16 Jun 2020 09:48:53: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:48:53: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:48:55: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:48:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:48:55: Process for pairing-model is terminated! INFO @ Tue, 16 Jun 2020 09:48:56: 33000000 cut: /home/okishinya/chipatlas/results/ce11/SRX494883/SRX494883.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494883/SRX494883.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494883/SRX494883.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494883/SRX494883.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:49:03: 34000000 INFO @ Tue, 16 Jun 2020 09:49:09: 35000000 INFO @ Tue, 16 Jun 2020 09:49:16: 36000000 INFO @ Tue, 16 Jun 2020 09:49:20: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:49:20: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:49:20: #1 total tags in treatment: 36623164 INFO @ Tue, 16 Jun 2020 09:49:20: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:49:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:49:21: #1 tags after filtering in treatment: 36623164 INFO @ Tue, 16 Jun 2020 09:49:21: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:49:21: #1 finished! INFO @ Tue, 16 Jun 2020 09:49:21: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:49:21: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:49:23: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:49:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:49:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX494883/SRX494883.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494883/SRX494883.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494883/SRX494883.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494883/SRX494883.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling