Job ID = 6368195 SRX = SRX494879 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:07:24 prefetch.2.10.7: 1) Downloading 'SRR1198411'... 2020-06-16T00:07:24 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:14:08 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:14:08 prefetch.2.10.7: 1) 'SRR1198411' was downloaded successfully Read 47950615 spots for SRR1198411/SRR1198411.sra Written 47950615 spots for SRR1198411/SRR1198411.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:46 47950615 reads; of these: 47950615 (100.00%) were unpaired; of these: 3138674 (6.55%) aligned 0 times 37485989 (78.18%) aligned exactly 1 time 7325952 (15.28%) aligned >1 times 93.45% overall alignment rate Time searching: 00:10:46 Overall time: 00:10:46 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8189000 / 44811941 = 0.1827 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:37:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX494879/SRX494879.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX494879/SRX494879.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX494879/SRX494879.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX494879/SRX494879.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:37:42: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:37:42: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:37:47: 1000000 INFO @ Tue, 16 Jun 2020 09:37:53: 2000000 INFO @ Tue, 16 Jun 2020 09:37:58: 3000000 INFO @ Tue, 16 Jun 2020 09:38:04: 4000000 INFO @ Tue, 16 Jun 2020 09:38:09: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:38:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX494879/SRX494879.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX494879/SRX494879.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX494879/SRX494879.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX494879/SRX494879.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:38:12: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:38:12: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:38:15: 6000000 INFO @ Tue, 16 Jun 2020 09:38:18: 1000000 INFO @ Tue, 16 Jun 2020 09:38:21: 7000000 INFO @ Tue, 16 Jun 2020 09:38:24: 2000000 INFO @ Tue, 16 Jun 2020 09:38:27: 8000000 INFO @ Tue, 16 Jun 2020 09:38:30: 3000000 INFO @ Tue, 16 Jun 2020 09:38:33: 9000000 INFO @ Tue, 16 Jun 2020 09:38:36: 4000000 INFO @ Tue, 16 Jun 2020 09:38:38: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:38:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX494879/SRX494879.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX494879/SRX494879.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX494879/SRX494879.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX494879/SRX494879.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:38:42: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:38:42: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:38:42: 5000000 INFO @ Tue, 16 Jun 2020 09:38:44: 11000000 INFO @ Tue, 16 Jun 2020 09:38:48: 1000000 INFO @ Tue, 16 Jun 2020 09:38:48: 6000000 INFO @ Tue, 16 Jun 2020 09:38:50: 12000000 INFO @ Tue, 16 Jun 2020 09:38:54: 2000000 INFO @ Tue, 16 Jun 2020 09:38:54: 7000000 INFO @ Tue, 16 Jun 2020 09:38:56: 13000000 INFO @ Tue, 16 Jun 2020 09:39:00: 3000000 INFO @ Tue, 16 Jun 2020 09:39:00: 8000000 INFO @ Tue, 16 Jun 2020 09:39:02: 14000000 INFO @ Tue, 16 Jun 2020 09:39:06: 4000000 INFO @ Tue, 16 Jun 2020 09:39:06: 9000000 INFO @ Tue, 16 Jun 2020 09:39:08: 15000000 INFO @ Tue, 16 Jun 2020 09:39:11: 5000000 INFO @ Tue, 16 Jun 2020 09:39:12: 10000000 INFO @ Tue, 16 Jun 2020 09:39:14: 16000000 INFO @ Tue, 16 Jun 2020 09:39:17: 6000000 INFO @ Tue, 16 Jun 2020 09:39:18: 11000000 INFO @ Tue, 16 Jun 2020 09:39:20: 17000000 INFO @ Tue, 16 Jun 2020 09:39:23: 7000000 INFO @ Tue, 16 Jun 2020 09:39:24: 12000000 INFO @ Tue, 16 Jun 2020 09:39:26: 18000000 INFO @ Tue, 16 Jun 2020 09:39:29: 8000000 INFO @ Tue, 16 Jun 2020 09:39:30: 13000000 INFO @ Tue, 16 Jun 2020 09:39:32: 19000000 INFO @ Tue, 16 Jun 2020 09:39:35: 9000000 INFO @ Tue, 16 Jun 2020 09:39:36: 14000000 INFO @ Tue, 16 Jun 2020 09:39:38: 20000000 INFO @ Tue, 16 Jun 2020 09:39:41: 10000000 INFO @ Tue, 16 Jun 2020 09:39:42: 15000000 INFO @ Tue, 16 Jun 2020 09:39:44: 21000000 INFO @ Tue, 16 Jun 2020 09:39:47: 11000000 INFO @ Tue, 16 Jun 2020 09:39:48: 16000000 INFO @ Tue, 16 Jun 2020 09:39:50: 22000000 INFO @ Tue, 16 Jun 2020 09:39:53: 12000000 INFO @ Tue, 16 Jun 2020 09:39:54: 17000000 INFO @ Tue, 16 Jun 2020 09:39:56: 23000000 INFO @ Tue, 16 Jun 2020 09:39:59: 13000000 INFO @ Tue, 16 Jun 2020 09:40:00: 18000000 INFO @ Tue, 16 Jun 2020 09:40:02: 24000000 INFO @ Tue, 16 Jun 2020 09:40:05: 14000000 INFO @ Tue, 16 Jun 2020 09:40:06: 19000000 INFO @ Tue, 16 Jun 2020 09:40:08: 25000000 INFO @ Tue, 16 Jun 2020 09:40:11: 15000000 INFO @ Tue, 16 Jun 2020 09:40:12: 20000000 INFO @ Tue, 16 Jun 2020 09:40:14: 26000000 INFO @ Tue, 16 Jun 2020 09:40:17: 16000000 INFO @ Tue, 16 Jun 2020 09:40:18: 21000000 INFO @ Tue, 16 Jun 2020 09:40:20: 27000000 INFO @ Tue, 16 Jun 2020 09:40:23: 17000000 INFO @ Tue, 16 Jun 2020 09:40:24: 22000000 INFO @ Tue, 16 Jun 2020 09:40:26: 28000000 INFO @ Tue, 16 Jun 2020 09:40:29: 18000000 INFO @ Tue, 16 Jun 2020 09:40:30: 23000000 INFO @ Tue, 16 Jun 2020 09:40:32: 29000000 INFO @ Tue, 16 Jun 2020 09:40:35: 19000000 INFO @ Tue, 16 Jun 2020 09:40:36: 24000000 INFO @ Tue, 16 Jun 2020 09:40:38: 30000000 INFO @ Tue, 16 Jun 2020 09:40:41: 20000000 INFO @ Tue, 16 Jun 2020 09:40:42: 25000000 INFO @ Tue, 16 Jun 2020 09:40:44: 31000000 INFO @ Tue, 16 Jun 2020 09:40:47: 21000000 INFO @ Tue, 16 Jun 2020 09:40:48: 26000000 INFO @ Tue, 16 Jun 2020 09:40:50: 32000000 INFO @ Tue, 16 Jun 2020 09:40:54: 22000000 INFO @ Tue, 16 Jun 2020 09:40:54: 27000000 INFO @ Tue, 16 Jun 2020 09:40:56: 33000000 INFO @ Tue, 16 Jun 2020 09:41:00: 23000000 INFO @ Tue, 16 Jun 2020 09:41:01: 28000000 INFO @ Tue, 16 Jun 2020 09:41:03: 34000000 INFO @ Tue, 16 Jun 2020 09:41:07: 24000000 INFO @ Tue, 16 Jun 2020 09:41:07: 29000000 INFO @ Tue, 16 Jun 2020 09:41:09: 35000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:41:13: 25000000 INFO @ Tue, 16 Jun 2020 09:41:13: 30000000 INFO @ Tue, 16 Jun 2020 09:41:15: 36000000 INFO @ Tue, 16 Jun 2020 09:41:19: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:41:19: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:41:19: #1 total tags in treatment: 36622941 INFO @ Tue, 16 Jun 2020 09:41:19: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:41:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:41:20: 26000000 INFO @ Tue, 16 Jun 2020 09:41:20: 31000000 INFO @ Tue, 16 Jun 2020 09:41:20: #1 tags after filtering in treatment: 36622941 INFO @ Tue, 16 Jun 2020 09:41:20: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:41:20: #1 finished! INFO @ Tue, 16 Jun 2020 09:41:20: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:41:20: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:41:22: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:41:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:41:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX494879/SRX494879.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494879/SRX494879.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494879/SRX494879.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494879/SRX494879.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:41:26: 32000000 INFO @ Tue, 16 Jun 2020 09:41:26: 27000000 INFO @ Tue, 16 Jun 2020 09:41:32: 33000000 INFO @ Tue, 16 Jun 2020 09:41:33: 28000000 INFO @ Tue, 16 Jun 2020 09:41:39: 34000000 INFO @ Tue, 16 Jun 2020 09:41:39: 29000000 INFO @ Tue, 16 Jun 2020 09:41:45: 35000000 INFO @ Tue, 16 Jun 2020 09:41:45: 30000000 INFO @ Tue, 16 Jun 2020 09:41:52: 36000000 INFO @ Tue, 16 Jun 2020 09:41:52: 31000000 INFO @ Tue, 16 Jun 2020 09:41:56: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:41:56: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:41:56: #1 total tags in treatment: 36622941 INFO @ Tue, 16 Jun 2020 09:41:56: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:41:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:41:57: #1 tags after filtering in treatment: 36622941 INFO @ Tue, 16 Jun 2020 09:41:57: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:41:57: #1 finished! INFO @ Tue, 16 Jun 2020 09:41:57: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:41:57: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:41:59: 32000000 INFO @ Tue, 16 Jun 2020 09:41:59: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:41:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:41:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX494879/SRX494879.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494879/SRX494879.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494879/SRX494879.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494879/SRX494879.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:42:05: 33000000 INFO @ Tue, 16 Jun 2020 09:42:11: 34000000 INFO @ Tue, 16 Jun 2020 09:42:17: 35000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 09:42:23: 36000000 INFO @ Tue, 16 Jun 2020 09:42:27: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:42:27: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:42:27: #1 total tags in treatment: 36622941 INFO @ Tue, 16 Jun 2020 09:42:27: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:42:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:42:28: #1 tags after filtering in treatment: 36622941 INFO @ Tue, 16 Jun 2020 09:42:28: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:42:28: #1 finished! INFO @ Tue, 16 Jun 2020 09:42:28: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:42:28: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:42:31: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:42:31: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:42:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX494879/SRX494879.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494879/SRX494879.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494879/SRX494879.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494879/SRX494879.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling