Job ID = 6368191 SRX = SRX494875 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:05:46 prefetch.2.10.7: 1) Downloading 'SRR1198407'... 2020-06-16T00:05:46 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:12:13 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:12:13 prefetch.2.10.7: 1) 'SRR1198407' was downloaded successfully Read 47950615 spots for SRR1198407/SRR1198407.sra Written 47950615 spots for SRR1198407/SRR1198407.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:08 47950615 reads; of these: 47950615 (100.00%) were unpaired; of these: 3138700 (6.55%) aligned 0 times 37485806 (78.18%) aligned exactly 1 time 7326109 (15.28%) aligned >1 times 93.45% overall alignment rate Time searching: 00:11:08 Overall time: 00:11:08 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8189415 / 44811915 = 0.1828 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:36:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX494875/SRX494875.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX494875/SRX494875.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX494875/SRX494875.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX494875/SRX494875.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:36:49: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:36:49: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:36:56: 1000000 INFO @ Tue, 16 Jun 2020 09:37:03: 2000000 INFO @ Tue, 16 Jun 2020 09:37:10: 3000000 INFO @ Tue, 16 Jun 2020 09:37:17: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:37:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX494875/SRX494875.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX494875/SRX494875.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX494875/SRX494875.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX494875/SRX494875.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:37:19: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:37:19: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:37:25: 5000000 INFO @ Tue, 16 Jun 2020 09:37:27: 1000000 INFO @ Tue, 16 Jun 2020 09:37:32: 6000000 INFO @ Tue, 16 Jun 2020 09:37:34: 2000000 INFO @ Tue, 16 Jun 2020 09:37:39: 7000000 INFO @ Tue, 16 Jun 2020 09:37:41: 3000000 INFO @ Tue, 16 Jun 2020 09:37:47: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:37:49: 4000000 INFO @ Tue, 16 Jun 2020 09:37:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX494875/SRX494875.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX494875/SRX494875.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX494875/SRX494875.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX494875/SRX494875.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:37:49: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:37:49: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:37:55: 9000000 INFO @ Tue, 16 Jun 2020 09:37:56: 5000000 INFO @ Tue, 16 Jun 2020 09:37:57: 1000000 INFO @ Tue, 16 Jun 2020 09:38:02: 10000000 INFO @ Tue, 16 Jun 2020 09:38:03: 6000000 INFO @ Tue, 16 Jun 2020 09:38:05: 2000000 INFO @ Tue, 16 Jun 2020 09:38:10: 11000000 INFO @ Tue, 16 Jun 2020 09:38:11: 7000000 INFO @ Tue, 16 Jun 2020 09:38:12: 3000000 INFO @ Tue, 16 Jun 2020 09:38:17: 12000000 INFO @ Tue, 16 Jun 2020 09:38:18: 8000000 INFO @ Tue, 16 Jun 2020 09:38:20: 4000000 INFO @ Tue, 16 Jun 2020 09:38:24: 13000000 INFO @ Tue, 16 Jun 2020 09:38:26: 9000000 INFO @ Tue, 16 Jun 2020 09:38:28: 5000000 INFO @ Tue, 16 Jun 2020 09:38:32: 14000000 INFO @ Tue, 16 Jun 2020 09:38:33: 10000000 INFO @ Tue, 16 Jun 2020 09:38:36: 6000000 INFO @ Tue, 16 Jun 2020 09:38:39: 15000000 INFO @ Tue, 16 Jun 2020 09:38:40: 11000000 INFO @ Tue, 16 Jun 2020 09:38:43: 7000000 INFO @ Tue, 16 Jun 2020 09:38:46: 16000000 INFO @ Tue, 16 Jun 2020 09:38:48: 12000000 INFO @ Tue, 16 Jun 2020 09:38:51: 8000000 INFO @ Tue, 16 Jun 2020 09:38:54: 17000000 INFO @ Tue, 16 Jun 2020 09:38:55: 13000000 INFO @ Tue, 16 Jun 2020 09:38:58: 9000000 INFO @ Tue, 16 Jun 2020 09:39:01: 18000000 INFO @ Tue, 16 Jun 2020 09:39:03: 14000000 INFO @ Tue, 16 Jun 2020 09:39:06: 10000000 INFO @ Tue, 16 Jun 2020 09:39:08: 19000000 INFO @ Tue, 16 Jun 2020 09:39:10: 15000000 INFO @ Tue, 16 Jun 2020 09:39:13: 11000000 INFO @ Tue, 16 Jun 2020 09:39:15: 20000000 INFO @ Tue, 16 Jun 2020 09:39:17: 16000000 INFO @ Tue, 16 Jun 2020 09:39:21: 12000000 INFO @ Tue, 16 Jun 2020 09:39:23: 21000000 INFO @ Tue, 16 Jun 2020 09:39:25: 17000000 INFO @ Tue, 16 Jun 2020 09:39:28: 13000000 INFO @ Tue, 16 Jun 2020 09:39:30: 22000000 INFO @ Tue, 16 Jun 2020 09:39:32: 18000000 INFO @ Tue, 16 Jun 2020 09:39:35: 14000000 INFO @ Tue, 16 Jun 2020 09:39:37: 23000000 INFO @ Tue, 16 Jun 2020 09:39:39: 19000000 INFO @ Tue, 16 Jun 2020 09:39:42: 15000000 INFO @ Tue, 16 Jun 2020 09:39:45: 24000000 INFO @ Tue, 16 Jun 2020 09:39:46: 20000000 INFO @ Tue, 16 Jun 2020 09:39:49: 16000000 INFO @ Tue, 16 Jun 2020 09:39:52: 25000000 INFO @ Tue, 16 Jun 2020 09:39:54: 21000000 INFO @ Tue, 16 Jun 2020 09:39:57: 17000000 INFO @ Tue, 16 Jun 2020 09:39:59: 26000000 INFO @ Tue, 16 Jun 2020 09:40:01: 22000000 INFO @ Tue, 16 Jun 2020 09:40:04: 18000000 INFO @ Tue, 16 Jun 2020 09:40:06: 27000000 INFO @ Tue, 16 Jun 2020 09:40:08: 23000000 INFO @ Tue, 16 Jun 2020 09:40:11: 19000000 INFO @ Tue, 16 Jun 2020 09:40:14: 28000000 INFO @ Tue, 16 Jun 2020 09:40:15: 24000000 INFO @ Tue, 16 Jun 2020 09:40:19: 20000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:40:21: 29000000 INFO @ Tue, 16 Jun 2020 09:40:22: 25000000 INFO @ Tue, 16 Jun 2020 09:40:26: 21000000 INFO @ Tue, 16 Jun 2020 09:40:29: 30000000 INFO @ Tue, 16 Jun 2020 09:40:30: 26000000 INFO @ Tue, 16 Jun 2020 09:40:34: 22000000 INFO @ Tue, 16 Jun 2020 09:40:37: 31000000 INFO @ Tue, 16 Jun 2020 09:40:37: 27000000 INFO @ Tue, 16 Jun 2020 09:40:42: 23000000 INFO @ Tue, 16 Jun 2020 09:40:44: 32000000 INFO @ Tue, 16 Jun 2020 09:40:45: 28000000 INFO @ Tue, 16 Jun 2020 09:40:50: 24000000 INFO @ Tue, 16 Jun 2020 09:40:52: 33000000 INFO @ Tue, 16 Jun 2020 09:40:52: 29000000 INFO @ Tue, 16 Jun 2020 09:40:58: 25000000 INFO @ Tue, 16 Jun 2020 09:40:59: 34000000 INFO @ Tue, 16 Jun 2020 09:41:00: 30000000 INFO @ Tue, 16 Jun 2020 09:41:06: 26000000 INFO @ Tue, 16 Jun 2020 09:41:07: 35000000 INFO @ Tue, 16 Jun 2020 09:41:08: 31000000 INFO @ Tue, 16 Jun 2020 09:41:14: 27000000 INFO @ Tue, 16 Jun 2020 09:41:15: 36000000 INFO @ Tue, 16 Jun 2020 09:41:16: 32000000 INFO @ Tue, 16 Jun 2020 09:41:20: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:41:20: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:41:20: #1 total tags in treatment: 36622500 INFO @ Tue, 16 Jun 2020 09:41:20: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:41:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:41:21: #1 tags after filtering in treatment: 36622500 INFO @ Tue, 16 Jun 2020 09:41:21: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:41:21: #1 finished! INFO @ Tue, 16 Jun 2020 09:41:21: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:41:21: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:41:22: 28000000 INFO @ Tue, 16 Jun 2020 09:41:23: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:41:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:41:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX494875/SRX494875.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494875/SRX494875.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494875/SRX494875.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494875/SRX494875.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:41:24: 33000000 INFO @ Tue, 16 Jun 2020 09:41:29: 29000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 09:41:32: 34000000 INFO @ Tue, 16 Jun 2020 09:41:37: 30000000 INFO @ Tue, 16 Jun 2020 09:41:40: 35000000 INFO @ Tue, 16 Jun 2020 09:41:45: 31000000 INFO @ Tue, 16 Jun 2020 09:41:48: 36000000 INFO @ Tue, 16 Jun 2020 09:41:53: 32000000 INFO @ Tue, 16 Jun 2020 09:41:53: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:41:53: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:41:53: #1 total tags in treatment: 36622500 INFO @ Tue, 16 Jun 2020 09:41:53: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:41:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:41:54: #1 tags after filtering in treatment: 36622500 INFO @ Tue, 16 Jun 2020 09:41:54: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:41:54: #1 finished! INFO @ Tue, 16 Jun 2020 09:41:54: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:41:54: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:41:56: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:41:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:41:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX494875/SRX494875.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494875/SRX494875.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494875/SRX494875.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494875/SRX494875.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:42:01: 33000000 INFO @ Tue, 16 Jun 2020 09:42:08: 34000000 INFO @ Tue, 16 Jun 2020 09:42:15: 35000000 INFO @ Tue, 16 Jun 2020 09:42:22: 36000000 INFO @ Tue, 16 Jun 2020 09:42:27: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:42:27: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:42:27: #1 total tags in treatment: 36622500 INFO @ Tue, 16 Jun 2020 09:42:27: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:42:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:42:28: #1 tags after filtering in treatment: 36622500 INFO @ Tue, 16 Jun 2020 09:42:28: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:42:28: #1 finished! INFO @ Tue, 16 Jun 2020 09:42:28: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:42:28: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:42:30: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:42:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:42:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX494875/SRX494875.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494875/SRX494875.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494875/SRX494875.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494875/SRX494875.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling