Job ID = 6368185 SRX = SRX494869 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:10:21 prefetch.2.10.7: 1) Downloading 'SRR1198401'... 2020-06-16T00:10:21 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:16:18 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:16:18 prefetch.2.10.7: 1) 'SRR1198401' was downloaded successfully Read 47950615 spots for SRR1198401/SRR1198401.sra Written 47950615 spots for SRR1198401/SRR1198401.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:09 47950615 reads; of these: 47950615 (100.00%) were unpaired; of these: 3138659 (6.55%) aligned 0 times 37486012 (78.18%) aligned exactly 1 time 7325944 (15.28%) aligned >1 times 93.45% overall alignment rate Time searching: 00:10:09 Overall time: 00:10:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8188828 / 44811956 = 0.1827 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:41:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX494869/SRX494869.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX494869/SRX494869.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX494869/SRX494869.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX494869/SRX494869.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:41:59: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:41:59: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:42:07: 1000000 INFO @ Tue, 16 Jun 2020 09:42:13: 2000000 INFO @ Tue, 16 Jun 2020 09:42:20: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:42:27: 4000000 INFO @ Tue, 16 Jun 2020 09:42:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX494869/SRX494869.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX494869/SRX494869.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX494869/SRX494869.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX494869/SRX494869.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:42:29: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:42:29: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:42:34: 5000000 INFO @ Tue, 16 Jun 2020 09:42:37: 1000000 INFO @ Tue, 16 Jun 2020 09:42:41: 6000000 INFO @ Tue, 16 Jun 2020 09:42:45: 2000000 INFO @ Tue, 16 Jun 2020 09:42:48: 7000000 INFO @ Tue, 16 Jun 2020 09:42:54: 3000000 INFO @ Tue, 16 Jun 2020 09:42:55: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:42:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX494869/SRX494869.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX494869/SRX494869.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX494869/SRX494869.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX494869/SRX494869.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:42:59: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:42:59: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:43:01: 4000000 INFO @ Tue, 16 Jun 2020 09:43:02: 9000000 INFO @ Tue, 16 Jun 2020 09:43:08: 1000000 INFO @ Tue, 16 Jun 2020 09:43:09: 10000000 INFO @ Tue, 16 Jun 2020 09:43:09: 5000000 INFO @ Tue, 16 Jun 2020 09:43:16: 2000000 INFO @ Tue, 16 Jun 2020 09:43:16: 11000000 INFO @ Tue, 16 Jun 2020 09:43:17: 6000000 INFO @ Tue, 16 Jun 2020 09:43:23: 3000000 INFO @ Tue, 16 Jun 2020 09:43:24: 12000000 INFO @ Tue, 16 Jun 2020 09:43:24: 7000000 INFO @ Tue, 16 Jun 2020 09:43:31: 4000000 INFO @ Tue, 16 Jun 2020 09:43:31: 13000000 INFO @ Tue, 16 Jun 2020 09:43:32: 8000000 INFO @ Tue, 16 Jun 2020 09:43:38: 5000000 INFO @ Tue, 16 Jun 2020 09:43:39: 14000000 INFO @ Tue, 16 Jun 2020 09:43:40: 9000000 INFO @ Tue, 16 Jun 2020 09:43:45: 6000000 INFO @ Tue, 16 Jun 2020 09:43:46: 15000000 INFO @ Tue, 16 Jun 2020 09:43:47: 10000000 INFO @ Tue, 16 Jun 2020 09:43:52: 7000000 INFO @ Tue, 16 Jun 2020 09:43:53: 16000000 INFO @ Tue, 16 Jun 2020 09:43:55: 11000000 INFO @ Tue, 16 Jun 2020 09:44:00: 8000000 INFO @ Tue, 16 Jun 2020 09:44:00: 17000000 INFO @ Tue, 16 Jun 2020 09:44:03: 12000000 INFO @ Tue, 16 Jun 2020 09:44:07: 9000000 INFO @ Tue, 16 Jun 2020 09:44:08: 18000000 INFO @ Tue, 16 Jun 2020 09:44:10: 13000000 INFO @ Tue, 16 Jun 2020 09:44:14: 10000000 INFO @ Tue, 16 Jun 2020 09:44:15: 19000000 INFO @ Tue, 16 Jun 2020 09:44:18: 14000000 INFO @ Tue, 16 Jun 2020 09:44:21: 11000000 INFO @ Tue, 16 Jun 2020 09:44:22: 20000000 INFO @ Tue, 16 Jun 2020 09:44:26: 15000000 INFO @ Tue, 16 Jun 2020 09:44:28: 12000000 INFO @ Tue, 16 Jun 2020 09:44:29: 21000000 INFO @ Tue, 16 Jun 2020 09:44:34: 16000000 INFO @ Tue, 16 Jun 2020 09:44:35: 13000000 INFO @ Tue, 16 Jun 2020 09:44:36: 22000000 INFO @ Tue, 16 Jun 2020 09:44:42: 17000000 INFO @ Tue, 16 Jun 2020 09:44:42: 14000000 INFO @ Tue, 16 Jun 2020 09:44:43: 23000000 INFO @ Tue, 16 Jun 2020 09:44:49: 15000000 INFO @ Tue, 16 Jun 2020 09:44:50: 18000000 INFO @ Tue, 16 Jun 2020 09:44:50: 24000000 INFO @ Tue, 16 Jun 2020 09:44:57: 16000000 INFO @ Tue, 16 Jun 2020 09:44:57: 19000000 INFO @ Tue, 16 Jun 2020 09:44:57: 25000000 INFO @ Tue, 16 Jun 2020 09:45:04: 17000000 INFO @ Tue, 16 Jun 2020 09:45:05: 26000000 INFO @ Tue, 16 Jun 2020 09:45:05: 20000000 INFO @ Tue, 16 Jun 2020 09:45:11: 18000000 INFO @ Tue, 16 Jun 2020 09:45:12: 27000000 INFO @ Tue, 16 Jun 2020 09:45:13: 21000000 INFO @ Tue, 16 Jun 2020 09:45:18: 19000000 INFO @ Tue, 16 Jun 2020 09:45:19: 28000000 INFO @ Tue, 16 Jun 2020 09:45:20: 22000000 INFO @ Tue, 16 Jun 2020 09:45:25: 20000000 INFO @ Tue, 16 Jun 2020 09:45:26: 29000000 INFO @ Tue, 16 Jun 2020 09:45:28: 23000000 INFO @ Tue, 16 Jun 2020 09:45:32: 21000000 INFO @ Tue, 16 Jun 2020 09:45:33: 30000000 INFO @ Tue, 16 Jun 2020 09:45:36: 24000000 INFO @ Tue, 16 Jun 2020 09:45:40: 22000000 INFO @ Tue, 16 Jun 2020 09:45:40: 31000000 INFO @ Tue, 16 Jun 2020 09:45:43: 25000000 INFO @ Tue, 16 Jun 2020 09:45:47: 23000000 INFO @ Tue, 16 Jun 2020 09:45:47: 32000000 INFO @ Tue, 16 Jun 2020 09:45:51: 26000000 INFO @ Tue, 16 Jun 2020 09:45:54: 24000000 INFO @ Tue, 16 Jun 2020 09:45:54: 33000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:45:59: 27000000 INFO @ Tue, 16 Jun 2020 09:46:01: 25000000 INFO @ Tue, 16 Jun 2020 09:46:02: 34000000 INFO @ Tue, 16 Jun 2020 09:46:06: 28000000 INFO @ Tue, 16 Jun 2020 09:46:08: 26000000 INFO @ Tue, 16 Jun 2020 09:46:09: 35000000 INFO @ Tue, 16 Jun 2020 09:46:14: 29000000 INFO @ Tue, 16 Jun 2020 09:46:15: 27000000 INFO @ Tue, 16 Jun 2020 09:46:16: 36000000 INFO @ Tue, 16 Jun 2020 09:46:20: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:46:20: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:46:20: #1 total tags in treatment: 36623128 INFO @ Tue, 16 Jun 2020 09:46:20: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:46:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:46:21: #1 tags after filtering in treatment: 36623128 INFO @ Tue, 16 Jun 2020 09:46:21: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:46:21: #1 finished! INFO @ Tue, 16 Jun 2020 09:46:21: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:46:21: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:46:22: 30000000 INFO @ Tue, 16 Jun 2020 09:46:22: 28000000 INFO @ Tue, 16 Jun 2020 09:46:23: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:46:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:46:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX494869/SRX494869.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494869/SRX494869.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494869/SRX494869.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494869/SRX494869.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:46:29: 29000000 INFO @ Tue, 16 Jun 2020 09:46:29: 31000000 INFO @ Tue, 16 Jun 2020 09:46:35: 30000000 INFO @ Tue, 16 Jun 2020 09:46:37: 32000000 INFO @ Tue, 16 Jun 2020 09:46:42: 31000000 INFO @ Tue, 16 Jun 2020 09:46:45: 33000000 INFO @ Tue, 16 Jun 2020 09:46:49: 32000000 INFO @ Tue, 16 Jun 2020 09:46:53: 34000000 INFO @ Tue, 16 Jun 2020 09:46:56: 33000000 INFO @ Tue, 16 Jun 2020 09:47:00: 35000000 INFO @ Tue, 16 Jun 2020 09:47:02: 34000000 INFO @ Tue, 16 Jun 2020 09:47:09: 36000000 INFO @ Tue, 16 Jun 2020 09:47:09: 35000000 INFO @ Tue, 16 Jun 2020 09:47:13: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:47:13: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:47:13: #1 total tags in treatment: 36623128 INFO @ Tue, 16 Jun 2020 09:47:13: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:47:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:47:14: #1 tags after filtering in treatment: 36623128 INFO @ Tue, 16 Jun 2020 09:47:14: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:47:14: #1 finished! INFO @ Tue, 16 Jun 2020 09:47:14: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:47:14: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:47:16: 36000000 INFO @ Tue, 16 Jun 2020 09:47:16: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:47:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:47:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX494869/SRX494869.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494869/SRX494869.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494869/SRX494869.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494869/SRX494869.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:47:20: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:47:20: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:47:20: #1 total tags in treatment: 36623128 INFO @ Tue, 16 Jun 2020 09:47:20: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:47:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 09:47:21: #1 tags after filtering in treatment: 36623128 INFO @ Tue, 16 Jun 2020 09:47:21: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:47:21: #1 finished! INFO @ Tue, 16 Jun 2020 09:47:21: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:47:21: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:47:23: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:47:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:47:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX494869/SRX494869.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494869/SRX494869.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494869/SRX494869.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494869/SRX494869.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling