Job ID = 6368181 SRX = SRX494865 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:01:30 prefetch.2.10.7: 1) Downloading 'SRR1198397'... 2020-06-16T00:01:30 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:06:24 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:06:24 prefetch.2.10.7: 1) 'SRR1198397' was downloaded successfully Read 47950615 spots for SRR1198397/SRR1198397.sra Written 47950615 spots for SRR1198397/SRR1198397.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:10:50 47950615 reads; of these: 47950615 (100.00%) were unpaired; of these: 3138700 (6.55%) aligned 0 times 37485861 (78.18%) aligned exactly 1 time 7326054 (15.28%) aligned >1 times 93.45% overall alignment rate Time searching: 00:10:51 Overall time: 00:10:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8189322 / 44811915 = 0.1827 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:30:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX494865/SRX494865.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX494865/SRX494865.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX494865/SRX494865.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX494865/SRX494865.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:30:32: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:30:32: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:30:37: 1000000 INFO @ Tue, 16 Jun 2020 09:30:43: 2000000 INFO @ Tue, 16 Jun 2020 09:30:49: 3000000 INFO @ Tue, 16 Jun 2020 09:30:54: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:31:00: 5000000 INFO @ Tue, 16 Jun 2020 09:31:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX494865/SRX494865.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX494865/SRX494865.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX494865/SRX494865.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX494865/SRX494865.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:31:02: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:31:02: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:31:06: 6000000 INFO @ Tue, 16 Jun 2020 09:31:08: 1000000 INFO @ Tue, 16 Jun 2020 09:31:13: 7000000 INFO @ Tue, 16 Jun 2020 09:31:15: 2000000 INFO @ Tue, 16 Jun 2020 09:31:20: 8000000 INFO @ Tue, 16 Jun 2020 09:31:22: 3000000 INFO @ Tue, 16 Jun 2020 09:31:26: 9000000 INFO @ Tue, 16 Jun 2020 09:31:29: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:31:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX494865/SRX494865.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX494865/SRX494865.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX494865/SRX494865.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX494865/SRX494865.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:31:32: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:31:32: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:31:33: 10000000 INFO @ Tue, 16 Jun 2020 09:31:36: 5000000 INFO @ Tue, 16 Jun 2020 09:31:39: 1000000 INFO @ Tue, 16 Jun 2020 09:31:40: 11000000 INFO @ Tue, 16 Jun 2020 09:31:42: 6000000 INFO @ Tue, 16 Jun 2020 09:31:45: 2000000 INFO @ Tue, 16 Jun 2020 09:31:46: 12000000 INFO @ Tue, 16 Jun 2020 09:31:49: 7000000 INFO @ Tue, 16 Jun 2020 09:31:52: 3000000 INFO @ Tue, 16 Jun 2020 09:31:53: 13000000 INFO @ Tue, 16 Jun 2020 09:31:56: 8000000 INFO @ Tue, 16 Jun 2020 09:31:59: 4000000 INFO @ Tue, 16 Jun 2020 09:31:59: 14000000 INFO @ Tue, 16 Jun 2020 09:32:03: 9000000 INFO @ Tue, 16 Jun 2020 09:32:05: 5000000 INFO @ Tue, 16 Jun 2020 09:32:06: 15000000 INFO @ Tue, 16 Jun 2020 09:32:10: 10000000 INFO @ Tue, 16 Jun 2020 09:32:12: 6000000 INFO @ Tue, 16 Jun 2020 09:32:13: 16000000 INFO @ Tue, 16 Jun 2020 09:32:16: 11000000 INFO @ Tue, 16 Jun 2020 09:32:19: 7000000 INFO @ Tue, 16 Jun 2020 09:32:19: 17000000 INFO @ Tue, 16 Jun 2020 09:32:23: 12000000 INFO @ Tue, 16 Jun 2020 09:32:25: 8000000 INFO @ Tue, 16 Jun 2020 09:32:26: 18000000 INFO @ Tue, 16 Jun 2020 09:32:30: 13000000 INFO @ Tue, 16 Jun 2020 09:32:32: 9000000 INFO @ Tue, 16 Jun 2020 09:32:32: 19000000 INFO @ Tue, 16 Jun 2020 09:32:37: 14000000 INFO @ Tue, 16 Jun 2020 09:32:38: 10000000 INFO @ Tue, 16 Jun 2020 09:32:39: 20000000 INFO @ Tue, 16 Jun 2020 09:32:43: 15000000 INFO @ Tue, 16 Jun 2020 09:32:45: 11000000 INFO @ Tue, 16 Jun 2020 09:32:46: 21000000 INFO @ Tue, 16 Jun 2020 09:32:50: 16000000 INFO @ Tue, 16 Jun 2020 09:32:51: 12000000 INFO @ Tue, 16 Jun 2020 09:32:52: 22000000 INFO @ Tue, 16 Jun 2020 09:32:57: 17000000 INFO @ Tue, 16 Jun 2020 09:32:58: 13000000 INFO @ Tue, 16 Jun 2020 09:32:59: 23000000 INFO @ Tue, 16 Jun 2020 09:33:04: 18000000 INFO @ Tue, 16 Jun 2020 09:33:05: 14000000 INFO @ Tue, 16 Jun 2020 09:33:05: 24000000 INFO @ Tue, 16 Jun 2020 09:33:10: 19000000 INFO @ Tue, 16 Jun 2020 09:33:11: 15000000 INFO @ Tue, 16 Jun 2020 09:33:12: 25000000 INFO @ Tue, 16 Jun 2020 09:33:17: 20000000 INFO @ Tue, 16 Jun 2020 09:33:18: 16000000 INFO @ Tue, 16 Jun 2020 09:33:18: 26000000 INFO @ Tue, 16 Jun 2020 09:33:24: 21000000 INFO @ Tue, 16 Jun 2020 09:33:25: 17000000 INFO @ Tue, 16 Jun 2020 09:33:25: 27000000 INFO @ Tue, 16 Jun 2020 09:33:30: 22000000 INFO @ Tue, 16 Jun 2020 09:33:31: 18000000 INFO @ Tue, 16 Jun 2020 09:33:31: 28000000 INFO @ Tue, 16 Jun 2020 09:33:37: 23000000 INFO @ Tue, 16 Jun 2020 09:33:38: 29000000 INFO @ Tue, 16 Jun 2020 09:33:38: 19000000 INFO @ Tue, 16 Jun 2020 09:33:44: 24000000 INFO @ Tue, 16 Jun 2020 09:33:44: 30000000 INFO @ Tue, 16 Jun 2020 09:33:44: 20000000 INFO @ Tue, 16 Jun 2020 09:33:51: 25000000 INFO @ Tue, 16 Jun 2020 09:33:51: 31000000 INFO @ Tue, 16 Jun 2020 09:33:51: 21000000 INFO @ Tue, 16 Jun 2020 09:33:57: 26000000 INFO @ Tue, 16 Jun 2020 09:33:57: 32000000 INFO @ Tue, 16 Jun 2020 09:33:57: 22000000 INFO @ Tue, 16 Jun 2020 09:34:04: 27000000 INFO @ Tue, 16 Jun 2020 09:34:04: 33000000 INFO @ Tue, 16 Jun 2020 09:34:04: 23000000 INFO @ Tue, 16 Jun 2020 09:34:10: 34000000 INFO @ Tue, 16 Jun 2020 09:34:10: 28000000 INFO @ Tue, 16 Jun 2020 09:34:11: 24000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:34:16: 35000000 INFO @ Tue, 16 Jun 2020 09:34:17: 29000000 INFO @ Tue, 16 Jun 2020 09:34:17: 25000000 INFO @ Tue, 16 Jun 2020 09:34:23: 36000000 INFO @ Tue, 16 Jun 2020 09:34:23: 30000000 INFO @ Tue, 16 Jun 2020 09:34:23: 26000000 INFO @ Tue, 16 Jun 2020 09:34:27: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:34:27: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:34:27: #1 total tags in treatment: 36622593 INFO @ Tue, 16 Jun 2020 09:34:27: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:34:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:34:28: #1 tags after filtering in treatment: 36622593 INFO @ Tue, 16 Jun 2020 09:34:28: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:34:28: #1 finished! INFO @ Tue, 16 Jun 2020 09:34:28: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:34:28: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:34:30: 31000000 INFO @ Tue, 16 Jun 2020 09:34:30: 27000000 INFO @ Tue, 16 Jun 2020 09:34:30: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:34:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:34:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX494865/SRX494865.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494865/SRX494865.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494865/SRX494865.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494865/SRX494865.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:34:36: 32000000 INFO @ Tue, 16 Jun 2020 09:34:36: 28000000 INFO @ Tue, 16 Jun 2020 09:34:42: 29000000 INFO @ Tue, 16 Jun 2020 09:34:42: 33000000 INFO @ Tue, 16 Jun 2020 09:34:49: 30000000 INFO @ Tue, 16 Jun 2020 09:34:49: 34000000 INFO @ Tue, 16 Jun 2020 09:34:55: 31000000 INFO @ Tue, 16 Jun 2020 09:34:55: 35000000 INFO @ Tue, 16 Jun 2020 09:35:01: 32000000 INFO @ Tue, 16 Jun 2020 09:35:02: 36000000 INFO @ Tue, 16 Jun 2020 09:35:06: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:35:06: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:35:06: #1 total tags in treatment: 36622593 INFO @ Tue, 16 Jun 2020 09:35:06: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:35:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:35:07: #1 tags after filtering in treatment: 36622593 INFO @ Tue, 16 Jun 2020 09:35:07: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:35:07: #1 finished! INFO @ Tue, 16 Jun 2020 09:35:07: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:35:07: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:35:07: 33000000 INFO @ Tue, 16 Jun 2020 09:35:09: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:35:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:35:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX494865/SRX494865.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494865/SRX494865.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494865/SRX494865.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494865/SRX494865.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:35:13: 34000000 INFO @ Tue, 16 Jun 2020 09:35:19: 35000000 INFO @ Tue, 16 Jun 2020 09:35:25: 36000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 09:35:29: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:35:29: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:35:29: #1 total tags in treatment: 36622593 INFO @ Tue, 16 Jun 2020 09:35:29: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:35:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:35:30: #1 tags after filtering in treatment: 36622593 INFO @ Tue, 16 Jun 2020 09:35:30: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:35:30: #1 finished! INFO @ Tue, 16 Jun 2020 09:35:30: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:35:30: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:35:32: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:35:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:35:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX494865/SRX494865.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494865/SRX494865.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494865/SRX494865.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494865/SRX494865.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling