Job ID = 6368178 SRX = SRX494862 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:05:01 prefetch.2.10.7: 1) Downloading 'SRR1198394'... 2020-06-16T00:05:01 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:14:00 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:14:00 prefetch.2.10.7: 1) 'SRR1198394' was downloaded successfully Read 47950615 spots for SRR1198394/SRR1198394.sra Written 47950615 spots for SRR1198394/SRR1198394.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:47 47950615 reads; of these: 47950615 (100.00%) were unpaired; of these: 3138727 (6.55%) aligned 0 times 37486137 (78.18%) aligned exactly 1 time 7325751 (15.28%) aligned >1 times 93.45% overall alignment rate Time searching: 00:10:47 Overall time: 00:10:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8189652 / 44811888 = 0.1828 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:37:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX494862/SRX494862.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX494862/SRX494862.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX494862/SRX494862.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX494862/SRX494862.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:37:36: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:37:36: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:37:43: 1000000 INFO @ Tue, 16 Jun 2020 09:37:49: 2000000 INFO @ Tue, 16 Jun 2020 09:37:56: 3000000 INFO @ Tue, 16 Jun 2020 09:38:02: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:38:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX494862/SRX494862.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX494862/SRX494862.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX494862/SRX494862.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX494862/SRX494862.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:38:05: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:38:05: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:38:09: 5000000 INFO @ Tue, 16 Jun 2020 09:38:12: 1000000 INFO @ Tue, 16 Jun 2020 09:38:16: 6000000 INFO @ Tue, 16 Jun 2020 09:38:18: 2000000 INFO @ Tue, 16 Jun 2020 09:38:23: 7000000 INFO @ Tue, 16 Jun 2020 09:38:24: 3000000 INFO @ Tue, 16 Jun 2020 09:38:30: 8000000 INFO @ Tue, 16 Jun 2020 09:38:30: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:38:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX494862/SRX494862.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX494862/SRX494862.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX494862/SRX494862.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX494862/SRX494862.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:38:35: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:38:35: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:38:36: 5000000 INFO @ Tue, 16 Jun 2020 09:38:37: 9000000 INFO @ Tue, 16 Jun 2020 09:38:42: 1000000 INFO @ Tue, 16 Jun 2020 09:38:42: 6000000 INFO @ Tue, 16 Jun 2020 09:38:43: 10000000 INFO @ Tue, 16 Jun 2020 09:38:48: 2000000 INFO @ Tue, 16 Jun 2020 09:38:48: 7000000 INFO @ Tue, 16 Jun 2020 09:38:50: 11000000 INFO @ Tue, 16 Jun 2020 09:38:54: 3000000 INFO @ Tue, 16 Jun 2020 09:38:54: 8000000 INFO @ Tue, 16 Jun 2020 09:38:57: 12000000 INFO @ Tue, 16 Jun 2020 09:39:00: 4000000 INFO @ Tue, 16 Jun 2020 09:39:00: 9000000 INFO @ Tue, 16 Jun 2020 09:39:04: 13000000 INFO @ Tue, 16 Jun 2020 09:39:07: 10000000 INFO @ Tue, 16 Jun 2020 09:39:07: 5000000 INFO @ Tue, 16 Jun 2020 09:39:11: 14000000 INFO @ Tue, 16 Jun 2020 09:39:13: 6000000 INFO @ Tue, 16 Jun 2020 09:39:13: 11000000 INFO @ Tue, 16 Jun 2020 09:39:18: 15000000 INFO @ Tue, 16 Jun 2020 09:39:19: 12000000 INFO @ Tue, 16 Jun 2020 09:39:19: 7000000 INFO @ Tue, 16 Jun 2020 09:39:25: 16000000 INFO @ Tue, 16 Jun 2020 09:39:26: 8000000 INFO @ Tue, 16 Jun 2020 09:39:26: 13000000 INFO @ Tue, 16 Jun 2020 09:39:32: 9000000 INFO @ Tue, 16 Jun 2020 09:39:32: 14000000 INFO @ Tue, 16 Jun 2020 09:39:32: 17000000 INFO @ Tue, 16 Jun 2020 09:39:38: 10000000 INFO @ Tue, 16 Jun 2020 09:39:38: 15000000 INFO @ Tue, 16 Jun 2020 09:39:39: 18000000 INFO @ Tue, 16 Jun 2020 09:39:45: 11000000 INFO @ Tue, 16 Jun 2020 09:39:45: 16000000 INFO @ Tue, 16 Jun 2020 09:39:46: 19000000 INFO @ Tue, 16 Jun 2020 09:39:51: 12000000 INFO @ Tue, 16 Jun 2020 09:39:51: 17000000 INFO @ Tue, 16 Jun 2020 09:39:53: 20000000 INFO @ Tue, 16 Jun 2020 09:39:57: 13000000 INFO @ Tue, 16 Jun 2020 09:39:57: 18000000 INFO @ Tue, 16 Jun 2020 09:40:00: 21000000 INFO @ Tue, 16 Jun 2020 09:40:04: 14000000 INFO @ Tue, 16 Jun 2020 09:40:04: 19000000 INFO @ Tue, 16 Jun 2020 09:40:07: 22000000 INFO @ Tue, 16 Jun 2020 09:40:10: 20000000 INFO @ Tue, 16 Jun 2020 09:40:10: 15000000 INFO @ Tue, 16 Jun 2020 09:40:15: 23000000 INFO @ Tue, 16 Jun 2020 09:40:17: 21000000 INFO @ Tue, 16 Jun 2020 09:40:17: 16000000 INFO @ Tue, 16 Jun 2020 09:40:22: 24000000 INFO @ Tue, 16 Jun 2020 09:40:23: 17000000 INFO @ Tue, 16 Jun 2020 09:40:23: 22000000 INFO @ Tue, 16 Jun 2020 09:40:29: 25000000 INFO @ Tue, 16 Jun 2020 09:40:29: 18000000 INFO @ Tue, 16 Jun 2020 09:40:29: 23000000 INFO @ Tue, 16 Jun 2020 09:40:36: 19000000 INFO @ Tue, 16 Jun 2020 09:40:36: 24000000 INFO @ Tue, 16 Jun 2020 09:40:36: 26000000 INFO @ Tue, 16 Jun 2020 09:40:42: 20000000 INFO @ Tue, 16 Jun 2020 09:40:43: 25000000 INFO @ Tue, 16 Jun 2020 09:40:43: 27000000 INFO @ Tue, 16 Jun 2020 09:40:49: 21000000 INFO @ Tue, 16 Jun 2020 09:40:49: 26000000 INFO @ Tue, 16 Jun 2020 09:40:51: 28000000 INFO @ Tue, 16 Jun 2020 09:40:56: 22000000 INFO @ Tue, 16 Jun 2020 09:40:56: 27000000 INFO @ Tue, 16 Jun 2020 09:40:58: 29000000 INFO @ Tue, 16 Jun 2020 09:41:03: 23000000 INFO @ Tue, 16 Jun 2020 09:41:03: 28000000 INFO @ Tue, 16 Jun 2020 09:41:04: 30000000 INFO @ Tue, 16 Jun 2020 09:41:09: 24000000 INFO @ Tue, 16 Jun 2020 09:41:10: 29000000 INFO @ Tue, 16 Jun 2020 09:41:11: 31000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:41:16: 25000000 INFO @ Tue, 16 Jun 2020 09:41:17: 30000000 INFO @ Tue, 16 Jun 2020 09:41:18: 32000000 INFO @ Tue, 16 Jun 2020 09:41:23: 26000000 INFO @ Tue, 16 Jun 2020 09:41:25: 33000000 INFO @ Tue, 16 Jun 2020 09:41:25: 31000000 INFO @ Tue, 16 Jun 2020 09:41:30: 27000000 INFO @ Tue, 16 Jun 2020 09:41:32: 34000000 INFO @ Tue, 16 Jun 2020 09:41:33: 32000000 INFO @ Tue, 16 Jun 2020 09:41:37: 28000000 INFO @ Tue, 16 Jun 2020 09:41:39: 35000000 INFO @ Tue, 16 Jun 2020 09:41:40: 33000000 INFO @ Tue, 16 Jun 2020 09:41:45: 29000000 INFO @ Tue, 16 Jun 2020 09:41:45: 36000000 INFO @ Tue, 16 Jun 2020 09:41:48: 34000000 INFO @ Tue, 16 Jun 2020 09:41:50: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:41:50: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:41:50: #1 total tags in treatment: 36622236 INFO @ Tue, 16 Jun 2020 09:41:50: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:41:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:41:50: #1 tags after filtering in treatment: 36622236 INFO @ Tue, 16 Jun 2020 09:41:50: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:41:50: #1 finished! INFO @ Tue, 16 Jun 2020 09:41:50: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:41:50: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:41:52: 30000000 INFO @ Tue, 16 Jun 2020 09:41:52: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:41:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:41:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX494862/SRX494862.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494862/SRX494862.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494862/SRX494862.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494862/SRX494862.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:41:55: 35000000 INFO @ Tue, 16 Jun 2020 09:42:00: 31000000 INFO @ Tue, 16 Jun 2020 09:42:02: 36000000 INFO @ Tue, 16 Jun 2020 09:42:07: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:42:07: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:42:07: #1 total tags in treatment: 36622236 INFO @ Tue, 16 Jun 2020 09:42:07: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:42:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:42:07: 32000000 INFO @ Tue, 16 Jun 2020 09:42:07: #1 tags after filtering in treatment: 36622236 INFO @ Tue, 16 Jun 2020 09:42:07: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:42:07: #1 finished! INFO @ Tue, 16 Jun 2020 09:42:07: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:42:07: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:42:10: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:42:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:42:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX494862/SRX494862.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494862/SRX494862.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494862/SRX494862.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494862/SRX494862.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:42:13: 33000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 09:42:20: 34000000 INFO @ Tue, 16 Jun 2020 09:42:26: 35000000 INFO @ Tue, 16 Jun 2020 09:42:33: 36000000 INFO @ Tue, 16 Jun 2020 09:42:37: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:42:37: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:42:37: #1 total tags in treatment: 36622236 INFO @ Tue, 16 Jun 2020 09:42:37: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:42:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:42:37: #1 tags after filtering in treatment: 36622236 INFO @ Tue, 16 Jun 2020 09:42:37: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:42:37: #1 finished! INFO @ Tue, 16 Jun 2020 09:42:37: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:42:37: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:42:40: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:42:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:42:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX494862/SRX494862.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494862/SRX494862.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494862/SRX494862.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX494862/SRX494862.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling