Job ID = 6367935 SRX = SRX4200544 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:18:36 prefetch.2.10.7: 1) Downloading 'SRR7298010'... 2020-06-16T00:18:36 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:26:29 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:26:29 prefetch.2.10.7: 1) 'SRR7298010' was downloaded successfully Read 40251697 spots for SRR7298010/SRR7298010.sra Written 40251697 spots for SRR7298010/SRR7298010.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:08 40251697 reads; of these: 40251697 (100.00%) were unpaired; of these: 432353 (1.07%) aligned 0 times 33299655 (82.73%) aligned exactly 1 time 6519689 (16.20%) aligned >1 times 98.93% overall alignment rate Time searching: 00:14:08 Overall time: 00:14:08 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 14048470 / 39819344 = 0.3528 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:52:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4200544/SRX4200544.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4200544/SRX4200544.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4200544/SRX4200544.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4200544/SRX4200544.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:52:55: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:52:55: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:53:02: 1000000 INFO @ Tue, 16 Jun 2020 09:53:08: 2000000 INFO @ Tue, 16 Jun 2020 09:53:14: 3000000 INFO @ Tue, 16 Jun 2020 09:53:21: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:53:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4200544/SRX4200544.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4200544/SRX4200544.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4200544/SRX4200544.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4200544/SRX4200544.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:53:25: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:53:25: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:53:28: 5000000 INFO @ Tue, 16 Jun 2020 09:53:33: 1000000 INFO @ Tue, 16 Jun 2020 09:53:35: 6000000 INFO @ Tue, 16 Jun 2020 09:53:41: 2000000 INFO @ Tue, 16 Jun 2020 09:53:43: 7000000 INFO @ Tue, 16 Jun 2020 09:53:49: 3000000 INFO @ Tue, 16 Jun 2020 09:53:51: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:53:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4200544/SRX4200544.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4200544/SRX4200544.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4200544/SRX4200544.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4200544/SRX4200544.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:53:55: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:53:55: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:53:57: 4000000 INFO @ Tue, 16 Jun 2020 09:53:59: 9000000 INFO @ Tue, 16 Jun 2020 09:54:04: 1000000 INFO @ Tue, 16 Jun 2020 09:54:05: 5000000 INFO @ Tue, 16 Jun 2020 09:54:07: 10000000 INFO @ Tue, 16 Jun 2020 09:54:12: 2000000 INFO @ Tue, 16 Jun 2020 09:54:13: 6000000 INFO @ Tue, 16 Jun 2020 09:54:15: 11000000 INFO @ Tue, 16 Jun 2020 09:54:20: 3000000 INFO @ Tue, 16 Jun 2020 09:54:21: 7000000 INFO @ Tue, 16 Jun 2020 09:54:23: 12000000 INFO @ Tue, 16 Jun 2020 09:54:28: 8000000 INFO @ Tue, 16 Jun 2020 09:54:28: 4000000 INFO @ Tue, 16 Jun 2020 09:54:30: 13000000 INFO @ Tue, 16 Jun 2020 09:54:36: 9000000 INFO @ Tue, 16 Jun 2020 09:54:37: 5000000 INFO @ Tue, 16 Jun 2020 09:54:38: 14000000 INFO @ Tue, 16 Jun 2020 09:54:44: 10000000 INFO @ Tue, 16 Jun 2020 09:54:44: 6000000 INFO @ Tue, 16 Jun 2020 09:54:46: 15000000 INFO @ Tue, 16 Jun 2020 09:54:52: 11000000 INFO @ Tue, 16 Jun 2020 09:54:52: 7000000 INFO @ Tue, 16 Jun 2020 09:54:54: 16000000 INFO @ Tue, 16 Jun 2020 09:54:59: 12000000 INFO @ Tue, 16 Jun 2020 09:55:00: 8000000 INFO @ Tue, 16 Jun 2020 09:55:01: 17000000 INFO @ Tue, 16 Jun 2020 09:55:07: 13000000 INFO @ Tue, 16 Jun 2020 09:55:08: 9000000 INFO @ Tue, 16 Jun 2020 09:55:09: 18000000 INFO @ Tue, 16 Jun 2020 09:55:15: 14000000 INFO @ Tue, 16 Jun 2020 09:55:16: 10000000 INFO @ Tue, 16 Jun 2020 09:55:17: 19000000 INFO @ Tue, 16 Jun 2020 09:55:22: 15000000 INFO @ Tue, 16 Jun 2020 09:55:23: 11000000 INFO @ Tue, 16 Jun 2020 09:55:25: 20000000 INFO @ Tue, 16 Jun 2020 09:55:30: 16000000 INFO @ Tue, 16 Jun 2020 09:55:31: 12000000 INFO @ Tue, 16 Jun 2020 09:55:32: 21000000 INFO @ Tue, 16 Jun 2020 09:55:38: 17000000 INFO @ Tue, 16 Jun 2020 09:55:39: 13000000 INFO @ Tue, 16 Jun 2020 09:55:40: 22000000 INFO @ Tue, 16 Jun 2020 09:55:45: 18000000 INFO @ Tue, 16 Jun 2020 09:55:47: 14000000 INFO @ Tue, 16 Jun 2020 09:55:48: 23000000 INFO @ Tue, 16 Jun 2020 09:55:53: 19000000 INFO @ Tue, 16 Jun 2020 09:55:54: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:55:55: 24000000 INFO @ Tue, 16 Jun 2020 09:56:01: 20000000 INFO @ Tue, 16 Jun 2020 09:56:02: 16000000 INFO @ Tue, 16 Jun 2020 09:56:03: 25000000 INFO @ Tue, 16 Jun 2020 09:56:08: 21000000 INFO @ Tue, 16 Jun 2020 09:56:09: #1 tag size is determined as 75 bps INFO @ Tue, 16 Jun 2020 09:56:09: #1 tag size = 75 INFO @ Tue, 16 Jun 2020 09:56:09: #1 total tags in treatment: 25770874 INFO @ Tue, 16 Jun 2020 09:56:09: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:56:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:56:09: #1 tags after filtering in treatment: 25770874 INFO @ Tue, 16 Jun 2020 09:56:09: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:56:09: #1 finished! INFO @ Tue, 16 Jun 2020 09:56:09: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:56:09: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:56:10: 17000000 INFO @ Tue, 16 Jun 2020 09:56:11: #2 number of paired peaks: 5 WARNING @ Tue, 16 Jun 2020 09:56:11: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:56:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX4200544/SRX4200544.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4200544/SRX4200544.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4200544/SRX4200544.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4200544/SRX4200544.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:56:16: 22000000 INFO @ Tue, 16 Jun 2020 09:56:18: 18000000 INFO @ Tue, 16 Jun 2020 09:56:23: 23000000 INFO @ Tue, 16 Jun 2020 09:56:25: 19000000 INFO @ Tue, 16 Jun 2020 09:56:31: 24000000 INFO @ Tue, 16 Jun 2020 09:56:33: 20000000 INFO @ Tue, 16 Jun 2020 09:56:38: 25000000 INFO @ Tue, 16 Jun 2020 09:56:40: 21000000 INFO @ Tue, 16 Jun 2020 09:56:44: #1 tag size is determined as 75 bps INFO @ Tue, 16 Jun 2020 09:56:44: #1 tag size = 75 INFO @ Tue, 16 Jun 2020 09:56:44: #1 total tags in treatment: 25770874 INFO @ Tue, 16 Jun 2020 09:56:44: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:56:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:56:45: #1 tags after filtering in treatment: 25770874 INFO @ Tue, 16 Jun 2020 09:56:45: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:56:45: #1 finished! INFO @ Tue, 16 Jun 2020 09:56:45: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:56:45: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:56:46: #2 number of paired peaks: 5 WARNING @ Tue, 16 Jun 2020 09:56:46: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:56:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX4200544/SRX4200544.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4200544/SRX4200544.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4200544/SRX4200544.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4200544/SRX4200544.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:56:48: 22000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 09:56:55: 23000000 INFO @ Tue, 16 Jun 2020 09:57:01: 24000000 INFO @ Tue, 16 Jun 2020 09:57:08: 25000000 INFO @ Tue, 16 Jun 2020 09:57:13: #1 tag size is determined as 75 bps INFO @ Tue, 16 Jun 2020 09:57:13: #1 tag size = 75 INFO @ Tue, 16 Jun 2020 09:57:13: #1 total tags in treatment: 25770874 INFO @ Tue, 16 Jun 2020 09:57:13: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:57:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:57:14: #1 tags after filtering in treatment: 25770874 INFO @ Tue, 16 Jun 2020 09:57:14: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:57:14: #1 finished! INFO @ Tue, 16 Jun 2020 09:57:14: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:57:14: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:57:15: #2 number of paired peaks: 5 WARNING @ Tue, 16 Jun 2020 09:57:15: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:57:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX4200544/SRX4200544.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4200544/SRX4200544.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4200544/SRX4200544.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4200544/SRX4200544.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling