Job ID = 6367929 SRX = SRX4200538 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:46:17 prefetch.2.10.7: 1) Downloading 'SRR7298004'... 2020-06-15T23:46:18 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:51:24 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:51:24 prefetch.2.10.7: 1) 'SRR7298004' was downloaded successfully Read 30593783 spots for SRR7298004/SRR7298004.sra Written 30593783 spots for SRR7298004/SRR7298004.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:43 30593783 reads; of these: 30593783 (100.00%) were unpaired; of these: 733883 (2.40%) aligned 0 times 25781929 (84.27%) aligned exactly 1 time 4077971 (13.33%) aligned >1 times 97.60% overall alignment rate Time searching: 00:10:43 Overall time: 00:10:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 15764038 / 29859900 = 0.5279 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:11:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4200538/SRX4200538.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4200538/SRX4200538.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4200538/SRX4200538.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4200538/SRX4200538.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:11:30: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:11:30: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:11:40: 1000000 INFO @ Tue, 16 Jun 2020 09:11:48: 2000000 INFO @ Tue, 16 Jun 2020 09:11:57: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:12:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4200538/SRX4200538.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4200538/SRX4200538.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4200538/SRX4200538.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4200538/SRX4200538.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:12:00: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:12:00: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:12:06: 4000000 INFO @ Tue, 16 Jun 2020 09:12:09: 1000000 INFO @ Tue, 16 Jun 2020 09:12:16: 5000000 INFO @ Tue, 16 Jun 2020 09:12:18: 2000000 INFO @ Tue, 16 Jun 2020 09:12:26: 6000000 INFO @ Tue, 16 Jun 2020 09:12:27: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:12:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4200538/SRX4200538.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4200538/SRX4200538.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4200538/SRX4200538.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4200538/SRX4200538.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:12:30: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:12:30: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:12:36: 7000000 INFO @ Tue, 16 Jun 2020 09:12:37: 4000000 INFO @ Tue, 16 Jun 2020 09:12:39: 1000000 INFO @ Tue, 16 Jun 2020 09:12:46: 5000000 INFO @ Tue, 16 Jun 2020 09:12:46: 8000000 INFO @ Tue, 16 Jun 2020 09:12:49: 2000000 INFO @ Tue, 16 Jun 2020 09:12:55: 6000000 INFO @ Tue, 16 Jun 2020 09:12:56: 9000000 INFO @ Tue, 16 Jun 2020 09:12:58: 3000000 INFO @ Tue, 16 Jun 2020 09:13:04: 7000000 INFO @ Tue, 16 Jun 2020 09:13:05: 10000000 INFO @ Tue, 16 Jun 2020 09:13:07: 4000000 INFO @ Tue, 16 Jun 2020 09:13:13: 8000000 INFO @ Tue, 16 Jun 2020 09:13:15: 11000000 INFO @ Tue, 16 Jun 2020 09:13:15: 5000000 INFO @ Tue, 16 Jun 2020 09:13:22: 9000000 INFO @ Tue, 16 Jun 2020 09:13:24: 12000000 INFO @ Tue, 16 Jun 2020 09:13:24: 6000000 INFO @ Tue, 16 Jun 2020 09:13:31: 10000000 INFO @ Tue, 16 Jun 2020 09:13:33: 7000000 INFO @ Tue, 16 Jun 2020 09:13:33: 13000000 INFO @ Tue, 16 Jun 2020 09:13:39: 11000000 INFO @ Tue, 16 Jun 2020 09:13:42: 8000000 INFO @ Tue, 16 Jun 2020 09:13:43: 14000000 INFO @ Tue, 16 Jun 2020 09:13:44: #1 tag size is determined as 75 bps INFO @ Tue, 16 Jun 2020 09:13:44: #1 tag size = 75 INFO @ Tue, 16 Jun 2020 09:13:44: #1 total tags in treatment: 14095862 INFO @ Tue, 16 Jun 2020 09:13:44: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:13:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:13:44: #1 tags after filtering in treatment: 14095862 INFO @ Tue, 16 Jun 2020 09:13:44: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:13:44: #1 finished! INFO @ Tue, 16 Jun 2020 09:13:44: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:13:44: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:13:45: #2 number of paired peaks: 55 WARNING @ Tue, 16 Jun 2020 09:13:45: Too few paired peaks (55) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:13:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX4200538/SRX4200538.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4200538/SRX4200538.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4200538/SRX4200538.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4200538/SRX4200538.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:13:48: 12000000 INFO @ Tue, 16 Jun 2020 09:13:50: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:13:56: 13000000 INFO @ Tue, 16 Jun 2020 09:13:58: 10000000 INFO @ Tue, 16 Jun 2020 09:14:04: 14000000 INFO @ Tue, 16 Jun 2020 09:14:04: #1 tag size is determined as 75 bps INFO @ Tue, 16 Jun 2020 09:14:04: #1 tag size = 75 INFO @ Tue, 16 Jun 2020 09:14:04: #1 total tags in treatment: 14095862 INFO @ Tue, 16 Jun 2020 09:14:04: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:14:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:14:05: #1 tags after filtering in treatment: 14095862 INFO @ Tue, 16 Jun 2020 09:14:05: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:14:05: #1 finished! INFO @ Tue, 16 Jun 2020 09:14:05: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:14:05: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:14:05: 11000000 INFO @ Tue, 16 Jun 2020 09:14:06: #2 number of paired peaks: 55 WARNING @ Tue, 16 Jun 2020 09:14:06: Too few paired peaks (55) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:14:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX4200538/SRX4200538.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4200538/SRX4200538.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4200538/SRX4200538.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4200538/SRX4200538.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:14:12: 12000000 INFO @ Tue, 16 Jun 2020 09:14:19: 13000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 09:14:26: 14000000 INFO @ Tue, 16 Jun 2020 09:14:27: #1 tag size is determined as 75 bps INFO @ Tue, 16 Jun 2020 09:14:27: #1 tag size = 75 INFO @ Tue, 16 Jun 2020 09:14:27: #1 total tags in treatment: 14095862 INFO @ Tue, 16 Jun 2020 09:14:27: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:14:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:14:27: #1 tags after filtering in treatment: 14095862 INFO @ Tue, 16 Jun 2020 09:14:27: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:14:27: #1 finished! INFO @ Tue, 16 Jun 2020 09:14:27: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:14:27: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:14:28: #2 number of paired peaks: 55 WARNING @ Tue, 16 Jun 2020 09:14:28: Too few paired peaks (55) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:14:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX4200538/SRX4200538.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4200538/SRX4200538.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4200538/SRX4200538.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4200538/SRX4200538.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling