Job ID = 6367928 SRX = SRX4200537 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:44:44 prefetch.2.10.7: 1) Downloading 'SRR7298003'... 2020-06-15T23:44:44 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:51:54 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:51:54 prefetch.2.10.7: 1) 'SRR7298003' was downloaded successfully Read 33370283 spots for SRR7298003/SRR7298003.sra Written 33370283 spots for SRR7298003/SRR7298003.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:05 33370283 reads; of these: 33370283 (100.00%) were unpaired; of these: 1319476 (3.95%) aligned 0 times 27824288 (83.38%) aligned exactly 1 time 4226519 (12.67%) aligned >1 times 96.05% overall alignment rate Time searching: 00:11:05 Overall time: 00:11:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 15699484 / 32050807 = 0.4898 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:13:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4200537/SRX4200537.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4200537/SRX4200537.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4200537/SRX4200537.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4200537/SRX4200537.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:13:00: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:13:00: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:13:07: 1000000 INFO @ Tue, 16 Jun 2020 09:13:13: 2000000 INFO @ Tue, 16 Jun 2020 09:13:20: 3000000 INFO @ Tue, 16 Jun 2020 09:13:27: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:13:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4200537/SRX4200537.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4200537/SRX4200537.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4200537/SRX4200537.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4200537/SRX4200537.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:13:29: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:13:29: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:13:34: 5000000 INFO @ Tue, 16 Jun 2020 09:13:37: 1000000 INFO @ Tue, 16 Jun 2020 09:13:41: 6000000 INFO @ Tue, 16 Jun 2020 09:13:44: 2000000 INFO @ Tue, 16 Jun 2020 09:13:48: 7000000 INFO @ Tue, 16 Jun 2020 09:13:52: 3000000 INFO @ Tue, 16 Jun 2020 09:13:55: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:13:59: 4000000 INFO @ Tue, 16 Jun 2020 09:14:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4200537/SRX4200537.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4200537/SRX4200537.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4200537/SRX4200537.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4200537/SRX4200537.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:14:00: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:14:00: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:14:03: 9000000 INFO @ Tue, 16 Jun 2020 09:14:07: 5000000 INFO @ Tue, 16 Jun 2020 09:14:07: 1000000 INFO @ Tue, 16 Jun 2020 09:14:10: 10000000 INFO @ Tue, 16 Jun 2020 09:14:14: 6000000 INFO @ Tue, 16 Jun 2020 09:14:15: 2000000 INFO @ Tue, 16 Jun 2020 09:14:17: 11000000 INFO @ Tue, 16 Jun 2020 09:14:21: 7000000 INFO @ Tue, 16 Jun 2020 09:14:22: 3000000 INFO @ Tue, 16 Jun 2020 09:14:25: 12000000 INFO @ Tue, 16 Jun 2020 09:14:29: 8000000 INFO @ Tue, 16 Jun 2020 09:14:30: 4000000 INFO @ Tue, 16 Jun 2020 09:14:32: 13000000 INFO @ Tue, 16 Jun 2020 09:14:36: 9000000 INFO @ Tue, 16 Jun 2020 09:14:37: 5000000 INFO @ Tue, 16 Jun 2020 09:14:40: 14000000 INFO @ Tue, 16 Jun 2020 09:14:44: 10000000 INFO @ Tue, 16 Jun 2020 09:14:44: 6000000 INFO @ Tue, 16 Jun 2020 09:14:47: 15000000 INFO @ Tue, 16 Jun 2020 09:14:51: 11000000 INFO @ Tue, 16 Jun 2020 09:14:52: 7000000 INFO @ Tue, 16 Jun 2020 09:14:55: 16000000 INFO @ Tue, 16 Jun 2020 09:14:57: #1 tag size is determined as 75 bps INFO @ Tue, 16 Jun 2020 09:14:57: #1 tag size = 75 INFO @ Tue, 16 Jun 2020 09:14:57: #1 total tags in treatment: 16351323 INFO @ Tue, 16 Jun 2020 09:14:57: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:14:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:14:57: #1 tags after filtering in treatment: 16351323 INFO @ Tue, 16 Jun 2020 09:14:57: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:14:57: #1 finished! INFO @ Tue, 16 Jun 2020 09:14:57: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:14:57: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:14:58: #2 number of paired peaks: 22 WARNING @ Tue, 16 Jun 2020 09:14:58: Too few paired peaks (22) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:14:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX4200537/SRX4200537.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4200537/SRX4200537.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4200537/SRX4200537.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4200537/SRX4200537.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:14:59: 12000000 INFO @ Tue, 16 Jun 2020 09:14:59: 8000000 INFO @ Tue, 16 Jun 2020 09:15:06: 13000000 INFO @ Tue, 16 Jun 2020 09:15:07: 9000000 INFO @ Tue, 16 Jun 2020 09:15:13: 14000000 INFO @ Tue, 16 Jun 2020 09:15:14: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:15:21: 15000000 INFO @ Tue, 16 Jun 2020 09:15:22: 11000000 INFO @ Tue, 16 Jun 2020 09:15:28: 16000000 INFO @ Tue, 16 Jun 2020 09:15:29: 12000000 INFO @ Tue, 16 Jun 2020 09:15:31: #1 tag size is determined as 75 bps INFO @ Tue, 16 Jun 2020 09:15:31: #1 tag size = 75 INFO @ Tue, 16 Jun 2020 09:15:31: #1 total tags in treatment: 16351323 INFO @ Tue, 16 Jun 2020 09:15:31: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:15:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:15:31: #1 tags after filtering in treatment: 16351323 INFO @ Tue, 16 Jun 2020 09:15:31: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:15:31: #1 finished! INFO @ Tue, 16 Jun 2020 09:15:31: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:15:31: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:15:32: #2 number of paired peaks: 22 WARNING @ Tue, 16 Jun 2020 09:15:32: Too few paired peaks (22) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:15:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX4200537/SRX4200537.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4200537/SRX4200537.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4200537/SRX4200537.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4200537/SRX4200537.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:15:36: 13000000 INFO @ Tue, 16 Jun 2020 09:15:43: 14000000 INFO @ Tue, 16 Jun 2020 09:15:50: 15000000 INFO @ Tue, 16 Jun 2020 09:15:57: 16000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 09:16:00: #1 tag size is determined as 75 bps INFO @ Tue, 16 Jun 2020 09:16:00: #1 tag size = 75 INFO @ Tue, 16 Jun 2020 09:16:00: #1 total tags in treatment: 16351323 INFO @ Tue, 16 Jun 2020 09:16:00: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:16:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:16:00: #1 tags after filtering in treatment: 16351323 INFO @ Tue, 16 Jun 2020 09:16:00: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:16:00: #1 finished! INFO @ Tue, 16 Jun 2020 09:16:00: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:16:00: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:16:01: #2 number of paired peaks: 22 WARNING @ Tue, 16 Jun 2020 09:16:01: Too few paired peaks (22) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:16:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX4200537/SRX4200537.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4200537/SRX4200537.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4200537/SRX4200537.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4200537/SRX4200537.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling