Job ID = 6367890 SRX = SRX4107873 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:38:14 prefetch.2.10.7: 1) Downloading 'SRR7191149'... 2020-06-15T23:38:14 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:39:53 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:39:53 prefetch.2.10.7: 'SRR7191149' is valid 2020-06-15T23:39:53 prefetch.2.10.7: 1) 'SRR7191149' was downloaded successfully 2020-06-15T23:41:13 prefetch.2.10.7: 'SRR7191149' has 16 unresolved dependencies 2020-06-15T23:41:13 prefetch.2.10.7: 2) Downloading 'ncbi-acc:BX284601.5?vdb-ctx=refseq'... 2020-06-15T23:41:13 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:41:29 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:41:29 prefetch.2.10.7: 2) 'ncbi-acc:BX284601.5?vdb-ctx=refseq' was downloaded successfully 2020-06-15T23:41:29 prefetch.2.10.7: 3) Downloading 'ncbi-acc:BX284602.5?vdb-ctx=refseq'... 2020-06-15T23:41:29 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:41:45 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:41:45 prefetch.2.10.7: 3) 'ncbi-acc:BX284602.5?vdb-ctx=refseq' was downloaded successfully 2020-06-15T23:41:45 prefetch.2.10.7: 4) Downloading 'ncbi-acc:BX284603.4?vdb-ctx=refseq'... 2020-06-15T23:41:45 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:42:03 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:42:03 prefetch.2.10.7: 4) 'ncbi-acc:BX284603.4?vdb-ctx=refseq' was downloaded successfully 2020-06-15T23:42:03 prefetch.2.10.7: 5) Downloading 'ncbi-acc:BX284604.4?vdb-ctx=refseq'... 2020-06-15T23:42:03 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:42:20 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:42:20 prefetch.2.10.7: 5) 'ncbi-acc:BX284604.4?vdb-ctx=refseq' was downloaded successfully 2020-06-15T23:42:20 prefetch.2.10.7: 6) Downloading 'ncbi-acc:BX284605.5?vdb-ctx=refseq'... 2020-06-15T23:42:20 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:42:36 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:42:36 prefetch.2.10.7: 6) 'ncbi-acc:BX284605.5?vdb-ctx=refseq' was downloaded successfully 2020-06-15T23:42:36 prefetch.2.10.7: 7) Downloading 'ncbi-acc:BX284606.5?vdb-ctx=refseq'... 2020-06-15T23:42:36 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:42:52 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:42:52 prefetch.2.10.7: 7) 'ncbi-acc:BX284606.5?vdb-ctx=refseq' was downloaded successfully 2020-06-15T23:42:52 prefetch.2.10.7: 8) Downloading 'ncbi-acc:NC_013486.1?vdb-ctx=refseq'... 2020-06-15T23:42:52 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:43:08 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:43:08 prefetch.2.10.7: 8) 'ncbi-acc:NC_013486.1?vdb-ctx=refseq' was downloaded successfully 2020-06-15T23:43:08 prefetch.2.10.7: 9) Downloading 'ncbi-acc:NC_013487.1?vdb-ctx=refseq'... 2020-06-15T23:43:08 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:43:24 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:43:24 prefetch.2.10.7: 9) 'ncbi-acc:NC_013487.1?vdb-ctx=refseq' was downloaded successfully 2020-06-15T23:43:24 prefetch.2.10.7: 10) Downloading 'ncbi-acc:NC_013488.1?vdb-ctx=refseq'... 2020-06-15T23:43:24 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:43:40 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:43:40 prefetch.2.10.7: 10) 'ncbi-acc:NC_013488.1?vdb-ctx=refseq' was downloaded successfully 2020-06-15T23:43:40 prefetch.2.10.7: 11) Downloading 'ncbi-acc:NC_013489.1?vdb-ctx=refseq'... 2020-06-15T23:43:40 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:43:57 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:43:57 prefetch.2.10.7: 11) 'ncbi-acc:NC_013489.1?vdb-ctx=refseq' was downloaded successfully 2020-06-15T23:43:57 prefetch.2.10.7: 12) Downloading 'ncbi-acc:NC_013490.1?vdb-ctx=refseq'... 2020-06-15T23:43:57 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:44:12 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:44:12 prefetch.2.10.7: 12) 'ncbi-acc:NC_013490.1?vdb-ctx=refseq' was downloaded successfully 2020-06-15T23:44:12 prefetch.2.10.7: 13) Downloading 'ncbi-acc:NC_013491.1?vdb-ctx=refseq'... 2020-06-15T23:44:12 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:44:28 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:44:28 prefetch.2.10.7: 13) 'ncbi-acc:NC_013491.1?vdb-ctx=refseq' was downloaded successfully 2020-06-15T23:44:28 prefetch.2.10.7: 14) Downloading 'ncbi-acc:NW_003101683.1?vdb-ctx=refseq'... 2020-06-15T23:44:28 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:44:41 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:44:41 prefetch.2.10.7: 14) 'ncbi-acc:NW_003101683.1?vdb-ctx=refseq' was downloaded successfully 2020-06-15T23:44:41 prefetch.2.10.7: 15) Downloading 'ncbi-acc:NW_003101684.1?vdb-ctx=refseq'... 2020-06-15T23:44:41 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:44:54 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:44:54 prefetch.2.10.7: 15) 'ncbi-acc:NW_003101684.1?vdb-ctx=refseq' was downloaded successfully 2020-06-15T23:44:54 prefetch.2.10.7: 16) Downloading 'ncbi-acc:NW_003101687.1?vdb-ctx=refseq'... 2020-06-15T23:44:54 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:45:08 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:45:08 prefetch.2.10.7: 16) 'ncbi-acc:NW_003101687.1?vdb-ctx=refseq' was downloaded successfully 2020-06-15T23:45:08 prefetch.2.10.7: 17) Downloading 'ncbi-acc:NW_003101688.1?vdb-ctx=refseq'... 2020-06-15T23:45:08 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:45:23 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:45:23 prefetch.2.10.7: 17) 'ncbi-acc:NW_003101688.1?vdb-ctx=refseq' was downloaded successfully Read 19084797 spots for SRR7191149/SRR7191149.sra Written 19084797 spots for SRR7191149/SRR7191149.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:59 19084797 reads; of these: 19084797 (100.00%) were unpaired; of these: 1324046 (6.94%) aligned 0 times 14837505 (77.75%) aligned exactly 1 time 2923246 (15.32%) aligned >1 times 93.06% overall alignment rate Time searching: 00:02:59 Overall time: 00:02:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2399708 / 17760751 = 0.1351 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:53:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4107873/SRX4107873.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4107873/SRX4107873.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4107873/SRX4107873.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4107873/SRX4107873.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:53:39: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:53:39: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:53:45: 1000000 INFO @ Tue, 16 Jun 2020 08:53:51: 2000000 INFO @ Tue, 16 Jun 2020 08:53:57: 3000000 INFO @ Tue, 16 Jun 2020 08:54:03: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:54:09: 5000000 INFO @ Tue, 16 Jun 2020 08:54:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4107873/SRX4107873.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4107873/SRX4107873.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4107873/SRX4107873.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4107873/SRX4107873.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:54:09: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:54:09: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:54:16: 1000000 INFO @ Tue, 16 Jun 2020 08:54:16: 6000000 INFO @ Tue, 16 Jun 2020 08:54:22: 2000000 INFO @ Tue, 16 Jun 2020 08:54:23: 7000000 INFO @ Tue, 16 Jun 2020 08:54:28: 3000000 INFO @ Tue, 16 Jun 2020 08:54:30: 8000000 INFO @ Tue, 16 Jun 2020 08:54:34: 4000000 INFO @ Tue, 16 Jun 2020 08:54:37: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:54:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4107873/SRX4107873.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4107873/SRX4107873.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4107873/SRX4107873.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4107873/SRX4107873.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:54:39: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:54:39: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:54:40: 5000000 INFO @ Tue, 16 Jun 2020 08:54:44: 10000000 INFO @ Tue, 16 Jun 2020 08:54:46: 1000000 INFO @ Tue, 16 Jun 2020 08:54:47: 6000000 INFO @ Tue, 16 Jun 2020 08:54:51: 11000000 INFO @ Tue, 16 Jun 2020 08:54:52: 2000000 INFO @ Tue, 16 Jun 2020 08:54:53: 7000000 INFO @ Tue, 16 Jun 2020 08:54:58: 3000000 INFO @ Tue, 16 Jun 2020 08:54:59: 12000000 INFO @ Tue, 16 Jun 2020 08:55:00: 8000000 INFO @ Tue, 16 Jun 2020 08:55:05: 4000000 INFO @ Tue, 16 Jun 2020 08:55:06: 13000000 INFO @ Tue, 16 Jun 2020 08:55:06: 9000000 INFO @ Tue, 16 Jun 2020 08:55:11: 5000000 INFO @ Tue, 16 Jun 2020 08:55:13: 10000000 INFO @ Tue, 16 Jun 2020 08:55:13: 14000000 INFO @ Tue, 16 Jun 2020 08:55:18: 6000000 INFO @ Tue, 16 Jun 2020 08:55:19: 11000000 INFO @ Tue, 16 Jun 2020 08:55:21: 15000000 INFO @ Tue, 16 Jun 2020 08:55:23: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 08:55:23: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 08:55:23: #1 total tags in treatment: 15361043 INFO @ Tue, 16 Jun 2020 08:55:23: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:55:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:55:24: #1 tags after filtering in treatment: 15361043 INFO @ Tue, 16 Jun 2020 08:55:24: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:55:24: #1 finished! INFO @ Tue, 16 Jun 2020 08:55:24: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:55:24: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:55:24: 7000000 INFO @ Tue, 16 Jun 2020 08:55:25: #2 number of paired peaks: 292 WARNING @ Tue, 16 Jun 2020 08:55:25: Fewer paired peaks (292) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 292 pairs to build model! INFO @ Tue, 16 Jun 2020 08:55:25: start model_add_line... INFO @ Tue, 16 Jun 2020 08:55:25: start X-correlation... INFO @ Tue, 16 Jun 2020 08:55:25: end of X-cor INFO @ Tue, 16 Jun 2020 08:55:25: #2 finished! INFO @ Tue, 16 Jun 2020 08:55:25: #2 predicted fragment length is 69 bps INFO @ Tue, 16 Jun 2020 08:55:25: #2 alternative fragment length(s) may be 4,69 bps INFO @ Tue, 16 Jun 2020 08:55:25: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX4107873/SRX4107873.05_model.r WARNING @ Tue, 16 Jun 2020 08:55:25: #2 Since the d (69) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 08:55:25: #2 You may need to consider one of the other alternative d(s): 4,69 WARNING @ Tue, 16 Jun 2020 08:55:25: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 08:55:25: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:55:25: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 08:55:25: 12000000 INFO @ Tue, 16 Jun 2020 08:55:30: 8000000 INFO @ Tue, 16 Jun 2020 08:55:32: 13000000 INFO @ Tue, 16 Jun 2020 08:55:37: 9000000 INFO @ Tue, 16 Jun 2020 08:55:38: 14000000 INFO @ Tue, 16 Jun 2020 08:55:43: 10000000 INFO @ Tue, 16 Jun 2020 08:55:45: 15000000 INFO @ Tue, 16 Jun 2020 08:55:47: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 08:55:47: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 08:55:47: #1 total tags in treatment: 15361043 INFO @ Tue, 16 Jun 2020 08:55:47: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:55:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:55:47: #1 tags after filtering in treatment: 15361043 INFO @ Tue, 16 Jun 2020 08:55:47: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:55:47: #1 finished! INFO @ Tue, 16 Jun 2020 08:55:47: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:55:47: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:55:48: #2 number of paired peaks: 292 WARNING @ Tue, 16 Jun 2020 08:55:48: Fewer paired peaks (292) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 292 pairs to build model! INFO @ Tue, 16 Jun 2020 08:55:48: start model_add_line... INFO @ Tue, 16 Jun 2020 08:55:48: start X-correlation... INFO @ Tue, 16 Jun 2020 08:55:48: end of X-cor INFO @ Tue, 16 Jun 2020 08:55:48: #2 finished! INFO @ Tue, 16 Jun 2020 08:55:48: #2 predicted fragment length is 69 bps INFO @ Tue, 16 Jun 2020 08:55:48: #2 alternative fragment length(s) may be 4,69 bps INFO @ Tue, 16 Jun 2020 08:55:48: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX4107873/SRX4107873.10_model.r WARNING @ Tue, 16 Jun 2020 08:55:48: #2 Since the d (69) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 08:55:48: #2 You may need to consider one of the other alternative d(s): 4,69 WARNING @ Tue, 16 Jun 2020 08:55:48: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 08:55:48: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:55:48: #3 Pre-compute pvalue-qvalue table... BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 08:55:49: 11000000 INFO @ Tue, 16 Jun 2020 08:55:54: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 08:55:55: 12000000 INFO @ Tue, 16 Jun 2020 08:56:01: 13000000 INFO @ Tue, 16 Jun 2020 08:56:07: 14000000 INFO @ Tue, 16 Jun 2020 08:56:10: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX4107873/SRX4107873.05_peaks.xls INFO @ Tue, 16 Jun 2020 08:56:10: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX4107873/SRX4107873.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:56:10: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX4107873/SRX4107873.05_summits.bed INFO @ Tue, 16 Jun 2020 08:56:10: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (6967 records, 4 fields): 8 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:56:13: 15000000 INFO @ Tue, 16 Jun 2020 08:56:15: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 08:56:15: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 08:56:15: #1 total tags in treatment: 15361043 INFO @ Tue, 16 Jun 2020 08:56:15: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:56:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:56:15: #1 tags after filtering in treatment: 15361043 INFO @ Tue, 16 Jun 2020 08:56:15: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:56:15: #1 finished! INFO @ Tue, 16 Jun 2020 08:56:15: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:56:15: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:56:16: #2 number of paired peaks: 292 WARNING @ Tue, 16 Jun 2020 08:56:16: Fewer paired peaks (292) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 292 pairs to build model! INFO @ Tue, 16 Jun 2020 08:56:16: start model_add_line... INFO @ Tue, 16 Jun 2020 08:56:16: start X-correlation... INFO @ Tue, 16 Jun 2020 08:56:16: end of X-cor INFO @ Tue, 16 Jun 2020 08:56:16: #2 finished! INFO @ Tue, 16 Jun 2020 08:56:16: #2 predicted fragment length is 69 bps INFO @ Tue, 16 Jun 2020 08:56:16: #2 alternative fragment length(s) may be 4,69 bps INFO @ Tue, 16 Jun 2020 08:56:16: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX4107873/SRX4107873.20_model.r WARNING @ Tue, 16 Jun 2020 08:56:16: #2 Since the d (69) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 08:56:16: #2 You may need to consider one of the other alternative d(s): 4,69 WARNING @ Tue, 16 Jun 2020 08:56:16: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 08:56:16: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:56:16: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 08:56:18: #3 Call peaks for each chromosome... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 08:56:33: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX4107873/SRX4107873.10_peaks.xls INFO @ Tue, 16 Jun 2020 08:56:33: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX4107873/SRX4107873.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:56:33: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX4107873/SRX4107873.10_summits.bed INFO @ Tue, 16 Jun 2020 08:56:33: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (3959 records, 4 fields): 8 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:56:45: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 08:56:58: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX4107873/SRX4107873.20_peaks.xls INFO @ Tue, 16 Jun 2020 08:56:58: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX4107873/SRX4107873.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:56:58: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX4107873/SRX4107873.20_summits.bed INFO @ Tue, 16 Jun 2020 08:56:58: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (1515 records, 4 fields): 2 millis CompletedMACS2peakCalling