Job ID = 12265550 SRX = SRX4082389 Genome = ce11 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:46:30 65190599 reads; of these: 65190599 (100.00%) were paired; of these: 5616166 (8.61%) aligned concordantly 0 times 53158288 (81.54%) aligned concordantly exactly 1 time 6416145 (9.84%) aligned concordantly >1 times ---- 5616166 pairs aligned concordantly 0 times; of these: 1189666 (21.18%) aligned discordantly 1 time ---- 4426500 pairs aligned 0 times concordantly or discordantly; of these: 8853000 mates make up the pairs; of these: 6294917 (71.10%) aligned 0 times 1954826 (22.08%) aligned exactly 1 time 603257 (6.81%) aligned >1 times 95.17% overall alignment rate Time searching: 00:46:31 Overall time: 00:46:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 52 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 28487965 / 58716979 = 0.4852 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:48:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4082389/SRX4082389.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4082389/SRX4082389.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4082389/SRX4082389.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4082389/SRX4082389.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:48:06: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:48:06: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:48:12: 1000000 INFO @ Sat, 03 Apr 2021 08:48:17: 2000000 INFO @ Sat, 03 Apr 2021 08:48:23: 3000000 INFO @ Sat, 03 Apr 2021 08:48:28: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:48:34: 5000000 INFO @ Sat, 03 Apr 2021 08:48:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4082389/SRX4082389.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4082389/SRX4082389.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4082389/SRX4082389.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4082389/SRX4082389.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:48:36: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:48:36: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:48:40: 6000000 INFO @ Sat, 03 Apr 2021 08:48:43: 1000000 INFO @ Sat, 03 Apr 2021 08:48:46: 7000000 INFO @ Sat, 03 Apr 2021 08:48:51: 2000000 INFO @ Sat, 03 Apr 2021 08:48:52: 8000000 INFO @ Sat, 03 Apr 2021 08:48:58: 9000000 INFO @ Sat, 03 Apr 2021 08:48:58: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:49:05: 10000000 INFO @ Sat, 03 Apr 2021 08:49:06: 4000000 INFO @ Sat, 03 Apr 2021 08:49:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4082389/SRX4082389.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4082389/SRX4082389.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4082389/SRX4082389.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4082389/SRX4082389.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:49:06: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:49:06: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:49:12: 11000000 INFO @ Sat, 03 Apr 2021 08:49:14: 5000000 INFO @ Sat, 03 Apr 2021 08:49:14: 1000000 INFO @ Sat, 03 Apr 2021 08:49:18: 12000000 INFO @ Sat, 03 Apr 2021 08:49:22: 6000000 INFO @ Sat, 03 Apr 2021 08:49:22: 2000000 INFO @ Sat, 03 Apr 2021 08:49:25: 13000000 INFO @ Sat, 03 Apr 2021 08:49:30: 7000000 INFO @ Sat, 03 Apr 2021 08:49:30: 3000000 INFO @ Sat, 03 Apr 2021 08:49:31: 14000000 INFO @ Sat, 03 Apr 2021 08:49:37: 15000000 INFO @ Sat, 03 Apr 2021 08:49:38: 8000000 INFO @ Sat, 03 Apr 2021 08:49:38: 4000000 INFO @ Sat, 03 Apr 2021 08:49:43: 16000000 INFO @ Sat, 03 Apr 2021 08:49:46: 9000000 INFO @ Sat, 03 Apr 2021 08:49:47: 5000000 INFO @ Sat, 03 Apr 2021 08:49:49: 17000000 INFO @ Sat, 03 Apr 2021 08:49:54: 10000000 INFO @ Sat, 03 Apr 2021 08:49:55: 6000000 INFO @ Sat, 03 Apr 2021 08:49:56: 18000000 INFO @ Sat, 03 Apr 2021 08:50:02: 19000000 INFO @ Sat, 03 Apr 2021 08:50:02: 11000000 INFO @ Sat, 03 Apr 2021 08:50:03: 7000000 INFO @ Sat, 03 Apr 2021 08:50:08: 20000000 INFO @ Sat, 03 Apr 2021 08:50:11: 8000000 INFO @ Sat, 03 Apr 2021 08:50:11: 12000000 INFO @ Sat, 03 Apr 2021 08:50:14: 21000000 INFO @ Sat, 03 Apr 2021 08:50:19: 9000000 INFO @ Sat, 03 Apr 2021 08:50:19: 13000000 INFO @ Sat, 03 Apr 2021 08:50:20: 22000000 INFO @ Sat, 03 Apr 2021 08:50:26: 23000000 INFO @ Sat, 03 Apr 2021 08:50:26: 14000000 INFO @ Sat, 03 Apr 2021 08:50:26: 10000000 INFO @ Sat, 03 Apr 2021 08:50:32: 24000000 INFO @ Sat, 03 Apr 2021 08:50:34: 15000000 INFO @ Sat, 03 Apr 2021 08:50:35: 11000000 INFO @ Sat, 03 Apr 2021 08:50:39: 25000000 INFO @ Sat, 03 Apr 2021 08:50:43: 16000000 INFO @ Sat, 03 Apr 2021 08:50:43: 12000000 INFO @ Sat, 03 Apr 2021 08:50:46: 26000000 INFO @ Sat, 03 Apr 2021 08:50:51: 17000000 INFO @ Sat, 03 Apr 2021 08:50:51: 13000000 INFO @ Sat, 03 Apr 2021 08:50:52: 27000000 INFO @ Sat, 03 Apr 2021 08:50:59: 28000000 INFO @ Sat, 03 Apr 2021 08:50:59: 18000000 INFO @ Sat, 03 Apr 2021 08:50:59: 14000000 INFO @ Sat, 03 Apr 2021 08:51:05: 29000000 INFO @ Sat, 03 Apr 2021 08:51:07: 19000000 INFO @ Sat, 03 Apr 2021 08:51:08: 15000000 INFO @ Sat, 03 Apr 2021 08:51:11: 30000000 INFO @ Sat, 03 Apr 2021 08:51:16: 20000000 INFO @ Sat, 03 Apr 2021 08:51:16: 16000000 INFO @ Sat, 03 Apr 2021 08:51:17: 31000000 INFO @ Sat, 03 Apr 2021 08:51:24: 32000000 INFO @ Sat, 03 Apr 2021 08:51:24: 21000000 INFO @ Sat, 03 Apr 2021 08:51:24: 17000000 INFO @ Sat, 03 Apr 2021 08:51:30: 33000000 INFO @ Sat, 03 Apr 2021 08:51:32: 22000000 INFO @ Sat, 03 Apr 2021 08:51:33: 18000000 INFO @ Sat, 03 Apr 2021 08:51:36: 34000000 INFO @ Sat, 03 Apr 2021 08:51:40: 23000000 INFO @ Sat, 03 Apr 2021 08:51:41: 19000000 INFO @ Sat, 03 Apr 2021 08:51:42: 35000000 INFO @ Sat, 03 Apr 2021 08:51:48: 36000000 INFO @ Sat, 03 Apr 2021 08:51:48: 24000000 INFO @ Sat, 03 Apr 2021 08:51:49: 20000000 INFO @ Sat, 03 Apr 2021 08:51:54: 37000000 INFO @ Sat, 03 Apr 2021 08:51:56: 25000000 INFO @ Sat, 03 Apr 2021 08:51:57: 21000000 INFO @ Sat, 03 Apr 2021 08:52:00: 38000000 INFO @ Sat, 03 Apr 2021 08:52:05: 26000000 INFO @ Sat, 03 Apr 2021 08:52:05: 22000000 INFO @ Sat, 03 Apr 2021 08:52:06: 39000000 INFO @ Sat, 03 Apr 2021 08:52:13: 27000000 INFO @ Sat, 03 Apr 2021 08:52:13: 40000000 INFO @ Sat, 03 Apr 2021 08:52:13: 23000000 INFO @ Sat, 03 Apr 2021 08:52:18: 41000000 INFO @ Sat, 03 Apr 2021 08:52:21: 28000000 INFO @ Sat, 03 Apr 2021 08:52:21: 24000000 INFO @ Sat, 03 Apr 2021 08:52:25: 42000000 INFO @ Sat, 03 Apr 2021 08:52:29: 29000000 INFO @ Sat, 03 Apr 2021 08:52:30: 25000000 INFO @ Sat, 03 Apr 2021 08:52:31: 43000000 INFO @ Sat, 03 Apr 2021 08:52:38: 30000000 INFO @ Sat, 03 Apr 2021 08:52:38: 44000000 INFO @ Sat, 03 Apr 2021 08:52:38: 26000000 INFO @ Sat, 03 Apr 2021 08:52:44: 45000000 INFO @ Sat, 03 Apr 2021 08:52:46: 31000000 INFO @ Sat, 03 Apr 2021 08:52:47: 27000000 INFO @ Sat, 03 Apr 2021 08:52:50: 46000000 INFO @ Sat, 03 Apr 2021 08:52:55: 32000000 INFO @ Sat, 03 Apr 2021 08:52:56: 28000000 INFO @ Sat, 03 Apr 2021 08:52:56: 47000000 INFO @ Sat, 03 Apr 2021 08:53:03: 48000000 INFO @ Sat, 03 Apr 2021 08:53:04: 33000000 INFO @ Sat, 03 Apr 2021 08:53:05: 29000000 INFO @ Sat, 03 Apr 2021 08:53:09: 49000000 INFO @ Sat, 03 Apr 2021 08:53:13: 34000000 INFO @ Sat, 03 Apr 2021 08:53:14: 30000000 INFO @ Sat, 03 Apr 2021 08:53:15: 50000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 08:53:21: 51000000 INFO @ Sat, 03 Apr 2021 08:53:22: 35000000 INFO @ Sat, 03 Apr 2021 08:53:23: 31000000 INFO @ Sat, 03 Apr 2021 08:53:28: 52000000 INFO @ Sat, 03 Apr 2021 08:53:32: 36000000 INFO @ Sat, 03 Apr 2021 08:53:32: 32000000 INFO @ Sat, 03 Apr 2021 08:53:35: 53000000 INFO @ Sat, 03 Apr 2021 08:53:41: 37000000 INFO @ Sat, 03 Apr 2021 08:53:41: 33000000 INFO @ Sat, 03 Apr 2021 08:53:43: 54000000 INFO @ Sat, 03 Apr 2021 08:53:50: 38000000 INFO @ Sat, 03 Apr 2021 08:53:51: 55000000 INFO @ Sat, 03 Apr 2021 08:53:51: 34000000 INFO @ Sat, 03 Apr 2021 08:53:59: 56000000 INFO @ Sat, 03 Apr 2021 08:53:59: 39000000 INFO @ Sat, 03 Apr 2021 08:54:00: 35000000 INFO @ Sat, 03 Apr 2021 08:54:06: 57000000 INFO @ Sat, 03 Apr 2021 08:54:08: 40000000 INFO @ Sat, 03 Apr 2021 08:54:09: 36000000 INFO @ Sat, 03 Apr 2021 08:54:14: 58000000 INFO @ Sat, 03 Apr 2021 08:54:18: 41000000 INFO @ Sat, 03 Apr 2021 08:54:18: 37000000 INFO @ Sat, 03 Apr 2021 08:54:21: 59000000 INFO @ Sat, 03 Apr 2021 08:54:26: 42000000 INFO @ Sat, 03 Apr 2021 08:54:27: 38000000 INFO @ Sat, 03 Apr 2021 08:54:28: 60000000 BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 08:54:35: 43000000 INFO @ Sat, 03 Apr 2021 08:54:36: 61000000 INFO @ Sat, 03 Apr 2021 08:54:36: 39000000 INFO @ Sat, 03 Apr 2021 08:54:43: 62000000 INFO @ Sat, 03 Apr 2021 08:54:45: 44000000 INFO @ Sat, 03 Apr 2021 08:54:45: 40000000 INFO @ Sat, 03 Apr 2021 08:54:49: 63000000 INFO @ Sat, 03 Apr 2021 08:54:54: 45000000 INFO @ Sat, 03 Apr 2021 08:54:54: 41000000 INFO @ Sat, 03 Apr 2021 08:54:55: 64000000 INFO @ Sat, 03 Apr 2021 08:55:01: 65000000 INFO @ Sat, 03 Apr 2021 08:55:02: 46000000 INFO @ Sat, 03 Apr 2021 08:55:03: 42000000 INFO @ Sat, 03 Apr 2021 08:55:07: 66000000 INFO @ Sat, 03 Apr 2021 08:55:11: 47000000 INFO @ Sat, 03 Apr 2021 08:55:12: 43000000 INFO @ Sat, 03 Apr 2021 08:55:13: 67000000 INFO @ Sat, 03 Apr 2021 08:55:14: #1 tag size is determined as 36 bps INFO @ Sat, 03 Apr 2021 08:55:14: #1 tag size = 36 INFO @ Sat, 03 Apr 2021 08:55:14: #1 total tags in treatment: 31229266 INFO @ Sat, 03 Apr 2021 08:55:14: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:55:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:55:14: #1 tags after filtering in treatment: 16448939 INFO @ Sat, 03 Apr 2021 08:55:14: #1 Redundant rate of treatment: 0.47 INFO @ Sat, 03 Apr 2021 08:55:14: #1 finished! INFO @ Sat, 03 Apr 2021 08:55:14: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:55:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:55:16: #2 number of paired peaks: 396 WARNING @ Sat, 03 Apr 2021 08:55:16: Fewer paired peaks (396) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 396 pairs to build model! INFO @ Sat, 03 Apr 2021 08:55:16: start model_add_line... INFO @ Sat, 03 Apr 2021 08:55:16: start X-correlation... INFO @ Sat, 03 Apr 2021 08:55:16: end of X-cor INFO @ Sat, 03 Apr 2021 08:55:16: #2 finished! INFO @ Sat, 03 Apr 2021 08:55:16: #2 predicted fragment length is 72 bps INFO @ Sat, 03 Apr 2021 08:55:16: #2 alternative fragment length(s) may be 72 bps INFO @ Sat, 03 Apr 2021 08:55:16: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX4082389/SRX4082389.05_model.r WARNING @ Sat, 03 Apr 2021 08:55:16: #2 Since the d (72) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 08:55:16: #2 You may need to consider one of the other alternative d(s): 72 WARNING @ Sat, 03 Apr 2021 08:55:16: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 08:55:16: #3 Call peaks... INFO @ Sat, 03 Apr 2021 08:55:16: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 08:55:20: 48000000 INFO @ Sat, 03 Apr 2021 08:55:21: 44000000 INFO @ Sat, 03 Apr 2021 08:55:29: 49000000 INFO @ Sat, 03 Apr 2021 08:55:30: 45000000 INFO @ Sat, 03 Apr 2021 08:55:37: 50000000 INFO @ Sat, 03 Apr 2021 08:55:38: 46000000 INFO @ Sat, 03 Apr 2021 08:55:45: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 08:55:46: 51000000 INFO @ Sat, 03 Apr 2021 08:55:46: 47000000 INFO @ Sat, 03 Apr 2021 08:55:54: 52000000 INFO @ Sat, 03 Apr 2021 08:55:54: 48000000 INFO @ Sat, 03 Apr 2021 08:56:02: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX4082389/SRX4082389.05_peaks.xls INFO @ Sat, 03 Apr 2021 08:56:02: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX4082389/SRX4082389.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 08:56:02: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX4082389/SRX4082389.05_summits.bed INFO @ Sat, 03 Apr 2021 08:56:02: Done! pass1 - making usageList (7 chroms): 3 millis pass2 - checking and writing primary data (13117 records, 4 fields): 16 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 08:56:02: 53000000 INFO @ Sat, 03 Apr 2021 08:56:03: 49000000 INFO @ Sat, 03 Apr 2021 08:56:11: 54000000 INFO @ Sat, 03 Apr 2021 08:56:11: 50000000 INFO @ Sat, 03 Apr 2021 08:56:19: 55000000 INFO @ Sat, 03 Apr 2021 08:56:19: 51000000 INFO @ Sat, 03 Apr 2021 08:56:27: 56000000 INFO @ Sat, 03 Apr 2021 08:56:28: 52000000 INFO @ Sat, 03 Apr 2021 08:56:35: 57000000 INFO @ Sat, 03 Apr 2021 08:56:36: 53000000 INFO @ Sat, 03 Apr 2021 08:56:43: 58000000 INFO @ Sat, 03 Apr 2021 08:56:44: 54000000 INFO @ Sat, 03 Apr 2021 08:56:52: 59000000 INFO @ Sat, 03 Apr 2021 08:56:52: 55000000 INFO @ Sat, 03 Apr 2021 08:57:00: 60000000 INFO @ Sat, 03 Apr 2021 08:57:01: 56000000 INFO @ Sat, 03 Apr 2021 08:57:08: 61000000 INFO @ Sat, 03 Apr 2021 08:57:09: 57000000 INFO @ Sat, 03 Apr 2021 08:57:16: 62000000 INFO @ Sat, 03 Apr 2021 08:57:17: 58000000 INFO @ Sat, 03 Apr 2021 08:57:24: 63000000 INFO @ Sat, 03 Apr 2021 08:57:25: 59000000 INFO @ Sat, 03 Apr 2021 08:57:33: 64000000 INFO @ Sat, 03 Apr 2021 08:57:33: 60000000 INFO @ Sat, 03 Apr 2021 08:57:41: 65000000 INFO @ Sat, 03 Apr 2021 08:57:41: 61000000 INFO @ Sat, 03 Apr 2021 08:57:49: 66000000 INFO @ Sat, 03 Apr 2021 08:57:49: 62000000 INFO @ Sat, 03 Apr 2021 08:57:56: 67000000 INFO @ Sat, 03 Apr 2021 08:57:57: 63000000 INFO @ Sat, 03 Apr 2021 08:57:58: #1 tag size is determined as 36 bps INFO @ Sat, 03 Apr 2021 08:57:58: #1 tag size = 36 INFO @ Sat, 03 Apr 2021 08:57:58: #1 total tags in treatment: 31229266 INFO @ Sat, 03 Apr 2021 08:57:58: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:57:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:57:58: #1 tags after filtering in treatment: 16448939 INFO @ Sat, 03 Apr 2021 08:57:58: #1 Redundant rate of treatment: 0.47 INFO @ Sat, 03 Apr 2021 08:57:58: #1 finished! INFO @ Sat, 03 Apr 2021 08:57:58: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:57:58: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:57:59: #2 number of paired peaks: 396 WARNING @ Sat, 03 Apr 2021 08:57:59: Fewer paired peaks (396) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 396 pairs to build model! INFO @ Sat, 03 Apr 2021 08:57:59: start model_add_line... INFO @ Sat, 03 Apr 2021 08:58:00: start X-correlation... INFO @ Sat, 03 Apr 2021 08:58:00: end of X-cor INFO @ Sat, 03 Apr 2021 08:58:00: #2 finished! INFO @ Sat, 03 Apr 2021 08:58:00: #2 predicted fragment length is 72 bps INFO @ Sat, 03 Apr 2021 08:58:00: #2 alternative fragment length(s) may be 72 bps INFO @ Sat, 03 Apr 2021 08:58:00: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX4082389/SRX4082389.10_model.r WARNING @ Sat, 03 Apr 2021 08:58:00: #2 Since the d (72) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 08:58:00: #2 You may need to consider one of the other alternative d(s): 72 WARNING @ Sat, 03 Apr 2021 08:58:00: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 08:58:00: #3 Call peaks... INFO @ Sat, 03 Apr 2021 08:58:00: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 08:58:04: 64000000 INFO @ Sat, 03 Apr 2021 08:58:12: 65000000 INFO @ Sat, 03 Apr 2021 08:58:19: 66000000 INFO @ Sat, 03 Apr 2021 08:58:26: 67000000 INFO @ Sat, 03 Apr 2021 08:58:27: #1 tag size is determined as 36 bps INFO @ Sat, 03 Apr 2021 08:58:27: #1 tag size = 36 INFO @ Sat, 03 Apr 2021 08:58:27: #1 total tags in treatment: 31229266 INFO @ Sat, 03 Apr 2021 08:58:27: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:58:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:58:28: #1 tags after filtering in treatment: 16448939 INFO @ Sat, 03 Apr 2021 08:58:28: #1 Redundant rate of treatment: 0.47 INFO @ Sat, 03 Apr 2021 08:58:28: #1 finished! INFO @ Sat, 03 Apr 2021 08:58:28: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:58:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:58:29: #2 number of paired peaks: 396 WARNING @ Sat, 03 Apr 2021 08:58:29: Fewer paired peaks (396) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 396 pairs to build model! INFO @ Sat, 03 Apr 2021 08:58:29: start model_add_line... INFO @ Sat, 03 Apr 2021 08:58:29: start X-correlation... INFO @ Sat, 03 Apr 2021 08:58:29: end of X-cor INFO @ Sat, 03 Apr 2021 08:58:29: #2 finished! INFO @ Sat, 03 Apr 2021 08:58:29: #2 predicted fragment length is 72 bps INFO @ Sat, 03 Apr 2021 08:58:29: #2 alternative fragment length(s) may be 72 bps INFO @ Sat, 03 Apr 2021 08:58:29: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX4082389/SRX4082389.20_model.r WARNING @ Sat, 03 Apr 2021 08:58:29: #2 Since the d (72) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 08:58:29: #2 You may need to consider one of the other alternative d(s): 72 WARNING @ Sat, 03 Apr 2021 08:58:29: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 08:58:29: #3 Call peaks... INFO @ Sat, 03 Apr 2021 08:58:29: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 08:58:30: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 08:58:47: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX4082389/SRX4082389.10_peaks.xls INFO @ Sat, 03 Apr 2021 08:58:47: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX4082389/SRX4082389.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 08:58:47: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX4082389/SRX4082389.10_summits.bed INFO @ Sat, 03 Apr 2021 08:58:47: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (8052 records, 4 fields): 11 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 08:58:59: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 08:59:16: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX4082389/SRX4082389.20_peaks.xls INFO @ Sat, 03 Apr 2021 08:59:16: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX4082389/SRX4082389.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 08:59:16: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX4082389/SRX4082389.20_summits.bed INFO @ Sat, 03 Apr 2021 08:59:16: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (3526 records, 4 fields): 6 millis CompletedMACS2peakCalling