Job ID = 6367742 SRX = SRX4082356 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:36:59 prefetch.2.10.7: 1) Downloading 'SRR7164174'... 2020-06-15T23:36:59 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:40:05 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:40:06 prefetch.2.10.7: 'SRR7164174' is valid 2020-06-15T23:40:06 prefetch.2.10.7: 1) 'SRR7164174' was downloaded successfully 2020-06-15T23:40:06 prefetch.2.10.7: 'SRR7164174' has 0 unresolved dependencies Read 19907659 spots for SRR7164174/SRR7164174.sra Written 19907659 spots for SRR7164174/SRR7164174.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:17 19907659 reads; of these: 19907659 (100.00%) were unpaired; of these: 344917 (1.73%) aligned 0 times 16882203 (84.80%) aligned exactly 1 time 2680539 (13.46%) aligned >1 times 98.27% overall alignment rate Time searching: 00:04:17 Overall time: 00:04:17 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3318552 / 19562742 = 0.1696 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:50:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4082356/SRX4082356.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4082356/SRX4082356.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4082356/SRX4082356.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4082356/SRX4082356.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:50:01: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:50:01: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:50:06: 1000000 INFO @ Tue, 16 Jun 2020 08:50:11: 2000000 INFO @ Tue, 16 Jun 2020 08:50:16: 3000000 INFO @ Tue, 16 Jun 2020 08:50:21: 4000000 INFO @ Tue, 16 Jun 2020 08:50:27: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:50:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4082356/SRX4082356.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4082356/SRX4082356.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4082356/SRX4082356.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4082356/SRX4082356.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:50:31: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:50:31: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:50:32: 6000000 INFO @ Tue, 16 Jun 2020 08:50:37: 1000000 INFO @ Tue, 16 Jun 2020 08:50:38: 7000000 INFO @ Tue, 16 Jun 2020 08:50:43: 2000000 INFO @ Tue, 16 Jun 2020 08:50:44: 8000000 INFO @ Tue, 16 Jun 2020 08:50:49: 3000000 INFO @ Tue, 16 Jun 2020 08:50:50: 9000000 INFO @ Tue, 16 Jun 2020 08:50:55: 4000000 INFO @ Tue, 16 Jun 2020 08:50:56: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:51:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4082356/SRX4082356.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4082356/SRX4082356.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4082356/SRX4082356.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4082356/SRX4082356.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:51:01: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:51:01: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:51:01: 5000000 INFO @ Tue, 16 Jun 2020 08:51:01: 11000000 INFO @ Tue, 16 Jun 2020 08:51:07: 6000000 INFO @ Tue, 16 Jun 2020 08:51:07: 1000000 INFO @ Tue, 16 Jun 2020 08:51:07: 12000000 INFO @ Tue, 16 Jun 2020 08:51:13: 7000000 INFO @ Tue, 16 Jun 2020 08:51:13: 13000000 INFO @ Tue, 16 Jun 2020 08:51:14: 2000000 INFO @ Tue, 16 Jun 2020 08:51:19: 14000000 INFO @ Tue, 16 Jun 2020 08:51:19: 8000000 INFO @ Tue, 16 Jun 2020 08:51:20: 3000000 INFO @ Tue, 16 Jun 2020 08:51:25: 15000000 INFO @ Tue, 16 Jun 2020 08:51:25: 9000000 INFO @ Tue, 16 Jun 2020 08:51:27: 4000000 INFO @ Tue, 16 Jun 2020 08:51:31: 16000000 INFO @ Tue, 16 Jun 2020 08:51:32: 10000000 INFO @ Tue, 16 Jun 2020 08:51:32: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 08:51:32: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 08:51:32: #1 total tags in treatment: 16244190 INFO @ Tue, 16 Jun 2020 08:51:32: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:51:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:51:33: #1 tags after filtering in treatment: 16244190 INFO @ Tue, 16 Jun 2020 08:51:33: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:51:33: #1 finished! INFO @ Tue, 16 Jun 2020 08:51:33: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:51:33: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:51:34: 5000000 INFO @ Tue, 16 Jun 2020 08:51:34: #2 number of paired peaks: 90 WARNING @ Tue, 16 Jun 2020 08:51:34: Too few paired peaks (90) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:51:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX4082356/SRX4082356.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4082356/SRX4082356.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4082356/SRX4082356.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4082356/SRX4082356.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:51:38: 11000000 INFO @ Tue, 16 Jun 2020 08:51:40: 6000000 INFO @ Tue, 16 Jun 2020 08:51:44: 12000000 INFO @ Tue, 16 Jun 2020 08:51:47: 7000000 INFO @ Tue, 16 Jun 2020 08:51:50: 13000000 INFO @ Tue, 16 Jun 2020 08:51:54: 8000000 INFO @ Tue, 16 Jun 2020 08:51:56: 14000000 INFO @ Tue, 16 Jun 2020 08:52:00: 9000000 INFO @ Tue, 16 Jun 2020 08:52:02: 15000000 INFO @ Tue, 16 Jun 2020 08:52:07: 10000000 INFO @ Tue, 16 Jun 2020 08:52:08: 16000000 INFO @ Tue, 16 Jun 2020 08:52:09: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 08:52:09: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 08:52:09: #1 total tags in treatment: 16244190 INFO @ Tue, 16 Jun 2020 08:52:09: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:52:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:52:10: #1 tags after filtering in treatment: 16244190 INFO @ Tue, 16 Jun 2020 08:52:10: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:52:10: #1 finished! INFO @ Tue, 16 Jun 2020 08:52:10: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:52:10: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:52:11: #2 number of paired peaks: 90 WARNING @ Tue, 16 Jun 2020 08:52:11: Too few paired peaks (90) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:52:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX4082356/SRX4082356.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4082356/SRX4082356.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4082356/SRX4082356.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4082356/SRX4082356.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:52:14: 11000000 INFO @ Tue, 16 Jun 2020 08:52:20: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 08:52:26: 13000000 INFO @ Tue, 16 Jun 2020 08:52:32: 14000000 INFO @ Tue, 16 Jun 2020 08:52:38: 15000000 INFO @ Tue, 16 Jun 2020 08:52:44: 16000000 INFO @ Tue, 16 Jun 2020 08:52:46: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 08:52:46: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 08:52:46: #1 total tags in treatment: 16244190 INFO @ Tue, 16 Jun 2020 08:52:46: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:52:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:52:46: #1 tags after filtering in treatment: 16244190 INFO @ Tue, 16 Jun 2020 08:52:46: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:52:46: #1 finished! INFO @ Tue, 16 Jun 2020 08:52:46: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:52:46: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:52:47: #2 number of paired peaks: 90 WARNING @ Tue, 16 Jun 2020 08:52:47: Too few paired peaks (90) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:52:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX4082356/SRX4082356.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4082356/SRX4082356.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4082356/SRX4082356.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX4082356/SRX4082356.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。