Job ID = 6367681 SRX = SRX3883436 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:37:59 prefetch.2.10.7: 1) Downloading 'SRR6938932'... 2020-06-15T23:37:59 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:48:33 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:48:33 prefetch.2.10.7: 1) 'SRR6938932' was downloaded successfully 2020-06-15T23:48:33 prefetch.2.10.7: 'SRR6938932' has 0 unresolved dependencies Read 91140648 spots for SRR6938932/SRR6938932.sra Written 91140648 spots for SRR6938932/SRR6938932.sra 2020-06-15T23:54:02 prefetch.2.10.7: 1) Downloading 'SRR6938933'... 2020-06-15T23:54:02 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:58:47 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:58:47 prefetch.2.10.7: 1) 'SRR6938933' was downloaded successfully 2020-06-15T23:58:47 prefetch.2.10.7: 'SRR6938933' has 0 unresolved dependencies Read 77772415 spots for SRR6938933/SRR6938933.sra Written 77772415 spots for SRR6938933/SRR6938933.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:25:30 168913063 reads; of these: 168913063 (100.00%) were unpaired; of these: 95036102 (56.26%) aligned 0 times 59813133 (35.41%) aligned exactly 1 time 14063828 (8.33%) aligned >1 times 43.74% overall alignment rate Time searching: 00:25:30 Overall time: 00:25:30 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 32479452 / 73876961 = 0.4396 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:44:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3883436/SRX3883436.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3883436/SRX3883436.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3883436/SRX3883436.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3883436/SRX3883436.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:44:52: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:44:52: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:44:57: 1000000 INFO @ Tue, 16 Jun 2020 09:45:02: 2000000 INFO @ Tue, 16 Jun 2020 09:45:07: 3000000 INFO @ Tue, 16 Jun 2020 09:45:11: 4000000 INFO @ Tue, 16 Jun 2020 09:45:16: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:45:21: 6000000 INFO @ Tue, 16 Jun 2020 09:45:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3883436/SRX3883436.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3883436/SRX3883436.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3883436/SRX3883436.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3883436/SRX3883436.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:45:21: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:45:21: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:45:26: 7000000 INFO @ Tue, 16 Jun 2020 09:45:27: 1000000 INFO @ Tue, 16 Jun 2020 09:45:31: 8000000 INFO @ Tue, 16 Jun 2020 09:45:33: 2000000 INFO @ Tue, 16 Jun 2020 09:45:36: 9000000 INFO @ Tue, 16 Jun 2020 09:45:39: 3000000 INFO @ Tue, 16 Jun 2020 09:45:41: 10000000 INFO @ Tue, 16 Jun 2020 09:45:44: 4000000 INFO @ Tue, 16 Jun 2020 09:45:46: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:45:50: 5000000 INFO @ Tue, 16 Jun 2020 09:45:51: 12000000 INFO @ Tue, 16 Jun 2020 09:45:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3883436/SRX3883436.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3883436/SRX3883436.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3883436/SRX3883436.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3883436/SRX3883436.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:45:51: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:45:51: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:45:56: 6000000 INFO @ Tue, 16 Jun 2020 09:45:56: 13000000 INFO @ Tue, 16 Jun 2020 09:45:57: 1000000 INFO @ Tue, 16 Jun 2020 09:46:01: 7000000 INFO @ Tue, 16 Jun 2020 09:46:01: 14000000 INFO @ Tue, 16 Jun 2020 09:46:02: 2000000 INFO @ Tue, 16 Jun 2020 09:46:06: 15000000 INFO @ Tue, 16 Jun 2020 09:46:07: 3000000 INFO @ Tue, 16 Jun 2020 09:46:07: 8000000 INFO @ Tue, 16 Jun 2020 09:46:11: 16000000 INFO @ Tue, 16 Jun 2020 09:46:12: 4000000 INFO @ Tue, 16 Jun 2020 09:46:13: 9000000 INFO @ Tue, 16 Jun 2020 09:46:16: 17000000 INFO @ Tue, 16 Jun 2020 09:46:17: 5000000 INFO @ Tue, 16 Jun 2020 09:46:18: 10000000 INFO @ Tue, 16 Jun 2020 09:46:21: 18000000 INFO @ Tue, 16 Jun 2020 09:46:22: 6000000 INFO @ Tue, 16 Jun 2020 09:46:24: 11000000 INFO @ Tue, 16 Jun 2020 09:46:27: 19000000 INFO @ Tue, 16 Jun 2020 09:46:27: 7000000 INFO @ Tue, 16 Jun 2020 09:46:30: 12000000 INFO @ Tue, 16 Jun 2020 09:46:32: 20000000 INFO @ Tue, 16 Jun 2020 09:46:32: 8000000 INFO @ Tue, 16 Jun 2020 09:46:35: 13000000 INFO @ Tue, 16 Jun 2020 09:46:37: 21000000 INFO @ Tue, 16 Jun 2020 09:46:37: 9000000 INFO @ Tue, 16 Jun 2020 09:46:41: 14000000 INFO @ Tue, 16 Jun 2020 09:46:42: 10000000 INFO @ Tue, 16 Jun 2020 09:46:42: 22000000 INFO @ Tue, 16 Jun 2020 09:46:46: 15000000 INFO @ Tue, 16 Jun 2020 09:46:47: 11000000 INFO @ Tue, 16 Jun 2020 09:46:47: 23000000 INFO @ Tue, 16 Jun 2020 09:46:52: 12000000 INFO @ Tue, 16 Jun 2020 09:46:52: 16000000 INFO @ Tue, 16 Jun 2020 09:46:53: 24000000 INFO @ Tue, 16 Jun 2020 09:46:57: 13000000 INFO @ Tue, 16 Jun 2020 09:46:58: 25000000 INFO @ Tue, 16 Jun 2020 09:46:58: 17000000 INFO @ Tue, 16 Jun 2020 09:47:02: 14000000 INFO @ Tue, 16 Jun 2020 09:47:03: 26000000 INFO @ Tue, 16 Jun 2020 09:47:04: 18000000 INFO @ Tue, 16 Jun 2020 09:47:06: 15000000 INFO @ Tue, 16 Jun 2020 09:47:08: 27000000 INFO @ Tue, 16 Jun 2020 09:47:09: 19000000 INFO @ Tue, 16 Jun 2020 09:47:11: 16000000 INFO @ Tue, 16 Jun 2020 09:47:13: 28000000 INFO @ Tue, 16 Jun 2020 09:47:15: 20000000 INFO @ Tue, 16 Jun 2020 09:47:16: 17000000 INFO @ Tue, 16 Jun 2020 09:47:18: 29000000 INFO @ Tue, 16 Jun 2020 09:47:21: 21000000 INFO @ Tue, 16 Jun 2020 09:47:21: 18000000 INFO @ Tue, 16 Jun 2020 09:47:23: 30000000 INFO @ Tue, 16 Jun 2020 09:47:26: 19000000 INFO @ Tue, 16 Jun 2020 09:47:27: 22000000 INFO @ Tue, 16 Jun 2020 09:47:28: 31000000 INFO @ Tue, 16 Jun 2020 09:47:31: 20000000 INFO @ Tue, 16 Jun 2020 09:47:32: 23000000 INFO @ Tue, 16 Jun 2020 09:47:33: 32000000 INFO @ Tue, 16 Jun 2020 09:47:36: 21000000 INFO @ Tue, 16 Jun 2020 09:47:38: 24000000 INFO @ Tue, 16 Jun 2020 09:47:39: 33000000 INFO @ Tue, 16 Jun 2020 09:47:41: 22000000 INFO @ Tue, 16 Jun 2020 09:47:43: 25000000 INFO @ Tue, 16 Jun 2020 09:47:44: 34000000 INFO @ Tue, 16 Jun 2020 09:47:46: 23000000 INFO @ Tue, 16 Jun 2020 09:47:49: 35000000 INFO @ Tue, 16 Jun 2020 09:47:49: 26000000 INFO @ Tue, 16 Jun 2020 09:47:51: 24000000 INFO @ Tue, 16 Jun 2020 09:47:54: 36000000 INFO @ Tue, 16 Jun 2020 09:47:54: 27000000 INFO @ Tue, 16 Jun 2020 09:47:56: 25000000 INFO @ Tue, 16 Jun 2020 09:47:59: 37000000 INFO @ Tue, 16 Jun 2020 09:48:00: 28000000 INFO @ Tue, 16 Jun 2020 09:48:01: 26000000 INFO @ Tue, 16 Jun 2020 09:48:04: 38000000 INFO @ Tue, 16 Jun 2020 09:48:06: 29000000 INFO @ Tue, 16 Jun 2020 09:48:06: 27000000 INFO @ Tue, 16 Jun 2020 09:48:09: 39000000 INFO @ Tue, 16 Jun 2020 09:48:11: 28000000 INFO @ Tue, 16 Jun 2020 09:48:12: 30000000 INFO @ Tue, 16 Jun 2020 09:48:14: 40000000 INFO @ Tue, 16 Jun 2020 09:48:17: 29000000 INFO @ Tue, 16 Jun 2020 09:48:17: 31000000 INFO @ Tue, 16 Jun 2020 09:48:19: 41000000 INFO @ Tue, 16 Jun 2020 09:48:21: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:48:21: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:48:21: #1 total tags in treatment: 41397509 INFO @ Tue, 16 Jun 2020 09:48:21: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:48:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:48:22: 30000000 INFO @ Tue, 16 Jun 2020 09:48:22: #1 tags after filtering in treatment: 41397509 INFO @ Tue, 16 Jun 2020 09:48:22: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:48:22: #1 finished! INFO @ Tue, 16 Jun 2020 09:48:22: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:48:22: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:48:23: 32000000 INFO @ Tue, 16 Jun 2020 09:48:25: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:48:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:48:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3883436/SRX3883436.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3883436/SRX3883436.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3883436/SRX3883436.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3883436/SRX3883436.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:48:27: 31000000 INFO @ Tue, 16 Jun 2020 09:48:29: 33000000 INFO @ Tue, 16 Jun 2020 09:48:32: 32000000 INFO @ Tue, 16 Jun 2020 09:48:34: 34000000 INFO @ Tue, 16 Jun 2020 09:48:37: 33000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:48:40: 35000000 INFO @ Tue, 16 Jun 2020 09:48:42: 34000000 INFO @ Tue, 16 Jun 2020 09:48:46: 36000000 INFO @ Tue, 16 Jun 2020 09:48:47: 35000000 INFO @ Tue, 16 Jun 2020 09:48:52: 37000000 INFO @ Tue, 16 Jun 2020 09:48:52: 36000000 INFO @ Tue, 16 Jun 2020 09:48:57: 37000000 INFO @ Tue, 16 Jun 2020 09:48:58: 38000000 INFO @ Tue, 16 Jun 2020 09:49:02: 38000000 INFO @ Tue, 16 Jun 2020 09:49:04: 39000000 INFO @ Tue, 16 Jun 2020 09:49:07: 39000000 INFO @ Tue, 16 Jun 2020 09:49:09: 40000000 INFO @ Tue, 16 Jun 2020 09:49:12: 40000000 INFO @ Tue, 16 Jun 2020 09:49:15: 41000000 INFO @ Tue, 16 Jun 2020 09:49:17: 41000000 INFO @ Tue, 16 Jun 2020 09:49:18: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:49:18: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:49:18: #1 total tags in treatment: 41397509 INFO @ Tue, 16 Jun 2020 09:49:18: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:49:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:49:18: #1 tags after filtering in treatment: 41397509 INFO @ Tue, 16 Jun 2020 09:49:18: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:49:18: #1 finished! INFO @ Tue, 16 Jun 2020 09:49:18: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:49:18: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:49:19: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:49:19: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:49:19: #1 total tags in treatment: 41397509 INFO @ Tue, 16 Jun 2020 09:49:19: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:49:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:49:20: #1 tags after filtering in treatment: 41397509 INFO @ Tue, 16 Jun 2020 09:49:20: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:49:20: #1 finished! INFO @ Tue, 16 Jun 2020 09:49:20: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:49:20: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:49:21: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:49:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:49:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3883436/SRX3883436.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3883436/SRX3883436.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3883436/SRX3883436.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3883436/SRX3883436.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:49:22: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:49:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:49:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3883436/SRX3883436.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3883436/SRX3883436.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3883436/SRX3883436.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3883436/SRX3883436.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。