Job ID = 6367635 SRX = SRX3583347 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:37:59 prefetch.2.10.7: 1) Downloading 'SRR6494003'... 2020-06-15T23:37:59 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:45:10 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:45:10 prefetch.2.10.7: 1) 'SRR6494003' was downloaded successfully 2020-06-15T23:45:10 prefetch.2.10.7: 'SRR6494003' has 0 unresolved dependencies Read 38102325 spots for SRR6494003/SRR6494003.sra Written 38102325 spots for SRR6494003/SRR6494003.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:46 38102325 reads; of these: 38102325 (100.00%) were unpaired; of these: 1221339 (3.21%) aligned 0 times 30903817 (81.11%) aligned exactly 1 time 5977169 (15.69%) aligned >1 times 96.79% overall alignment rate Time searching: 00:13:46 Overall time: 00:13:46 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 7704000 / 36880986 = 0.2089 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:10:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3583347/SRX3583347.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3583347/SRX3583347.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3583347/SRX3583347.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3583347/SRX3583347.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:10:10: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:10:10: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:10:18: 1000000 INFO @ Tue, 16 Jun 2020 09:10:25: 2000000 INFO @ Tue, 16 Jun 2020 09:10:32: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:10:38: 4000000 INFO @ Tue, 16 Jun 2020 09:10:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3583347/SRX3583347.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3583347/SRX3583347.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3583347/SRX3583347.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3583347/SRX3583347.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:10:40: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:10:40: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:10:46: 5000000 INFO @ Tue, 16 Jun 2020 09:10:48: 1000000 INFO @ Tue, 16 Jun 2020 09:10:53: 6000000 INFO @ Tue, 16 Jun 2020 09:10:55: 2000000 INFO @ Tue, 16 Jun 2020 09:11:00: 7000000 INFO @ Tue, 16 Jun 2020 09:11:02: 3000000 INFO @ Tue, 16 Jun 2020 09:11:08: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:11:10: 4000000 INFO @ Tue, 16 Jun 2020 09:11:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3583347/SRX3583347.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3583347/SRX3583347.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3583347/SRX3583347.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3583347/SRX3583347.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:11:10: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:11:10: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:11:15: 9000000 INFO @ Tue, 16 Jun 2020 09:11:18: 5000000 INFO @ Tue, 16 Jun 2020 09:11:18: 1000000 INFO @ Tue, 16 Jun 2020 09:11:23: 10000000 INFO @ Tue, 16 Jun 2020 09:11:25: 6000000 INFO @ Tue, 16 Jun 2020 09:11:26: 2000000 INFO @ Tue, 16 Jun 2020 09:11:31: 11000000 INFO @ Tue, 16 Jun 2020 09:11:33: 7000000 INFO @ Tue, 16 Jun 2020 09:11:33: 3000000 INFO @ Tue, 16 Jun 2020 09:11:38: 12000000 INFO @ Tue, 16 Jun 2020 09:11:41: 8000000 INFO @ Tue, 16 Jun 2020 09:11:41: 4000000 INFO @ Tue, 16 Jun 2020 09:11:46: 13000000 INFO @ Tue, 16 Jun 2020 09:11:48: 9000000 INFO @ Tue, 16 Jun 2020 09:11:49: 5000000 INFO @ Tue, 16 Jun 2020 09:11:53: 14000000 INFO @ Tue, 16 Jun 2020 09:11:56: 10000000 INFO @ Tue, 16 Jun 2020 09:11:56: 6000000 INFO @ Tue, 16 Jun 2020 09:12:01: 15000000 INFO @ Tue, 16 Jun 2020 09:12:03: 11000000 INFO @ Tue, 16 Jun 2020 09:12:04: 7000000 INFO @ Tue, 16 Jun 2020 09:12:08: 16000000 INFO @ Tue, 16 Jun 2020 09:12:11: 12000000 INFO @ Tue, 16 Jun 2020 09:12:12: 8000000 INFO @ Tue, 16 Jun 2020 09:12:16: 17000000 INFO @ Tue, 16 Jun 2020 09:12:19: 13000000 INFO @ Tue, 16 Jun 2020 09:12:19: 9000000 INFO @ Tue, 16 Jun 2020 09:12:23: 18000000 INFO @ Tue, 16 Jun 2020 09:12:26: 14000000 INFO @ Tue, 16 Jun 2020 09:12:27: 10000000 INFO @ Tue, 16 Jun 2020 09:12:31: 19000000 INFO @ Tue, 16 Jun 2020 09:12:34: 15000000 INFO @ Tue, 16 Jun 2020 09:12:34: 11000000 INFO @ Tue, 16 Jun 2020 09:12:38: 20000000 INFO @ Tue, 16 Jun 2020 09:12:41: 16000000 INFO @ Tue, 16 Jun 2020 09:12:42: 12000000 INFO @ Tue, 16 Jun 2020 09:12:46: 21000000 INFO @ Tue, 16 Jun 2020 09:12:49: 17000000 INFO @ Tue, 16 Jun 2020 09:12:49: 13000000 INFO @ Tue, 16 Jun 2020 09:12:53: 22000000 INFO @ Tue, 16 Jun 2020 09:12:56: 18000000 INFO @ Tue, 16 Jun 2020 09:12:57: 14000000 INFO @ Tue, 16 Jun 2020 09:13:01: 23000000 INFO @ Tue, 16 Jun 2020 09:13:04: 19000000 INFO @ Tue, 16 Jun 2020 09:13:05: 15000000 INFO @ Tue, 16 Jun 2020 09:13:08: 24000000 INFO @ Tue, 16 Jun 2020 09:13:11: 20000000 INFO @ Tue, 16 Jun 2020 09:13:12: 16000000 INFO @ Tue, 16 Jun 2020 09:13:16: 25000000 INFO @ Tue, 16 Jun 2020 09:13:19: 21000000 INFO @ Tue, 16 Jun 2020 09:13:20: 17000000 INFO @ Tue, 16 Jun 2020 09:13:23: 26000000 INFO @ Tue, 16 Jun 2020 09:13:27: 22000000 INFO @ Tue, 16 Jun 2020 09:13:28: 18000000 INFO @ Tue, 16 Jun 2020 09:13:31: 27000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:13:34: 23000000 INFO @ Tue, 16 Jun 2020 09:13:36: 19000000 INFO @ Tue, 16 Jun 2020 09:13:38: 28000000 INFO @ Tue, 16 Jun 2020 09:13:42: 24000000 INFO @ Tue, 16 Jun 2020 09:13:43: 20000000 INFO @ Tue, 16 Jun 2020 09:13:46: 29000000 INFO @ Tue, 16 Jun 2020 09:13:47: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 09:13:47: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 09:13:47: #1 total tags in treatment: 29176986 INFO @ Tue, 16 Jun 2020 09:13:47: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:13:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:13:48: #1 tags after filtering in treatment: 29176986 INFO @ Tue, 16 Jun 2020 09:13:48: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:13:48: #1 finished! INFO @ Tue, 16 Jun 2020 09:13:48: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:13:48: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:13:49: 25000000 INFO @ Tue, 16 Jun 2020 09:13:50: #2 number of paired peaks: 73 WARNING @ Tue, 16 Jun 2020 09:13:50: Too few paired peaks (73) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:13:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3583347/SRX3583347.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3583347/SRX3583347.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3583347/SRX3583347.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3583347/SRX3583347.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:13:51: 21000000 INFO @ Tue, 16 Jun 2020 09:13:57: 26000000 INFO @ Tue, 16 Jun 2020 09:13:58: 22000000 INFO @ Tue, 16 Jun 2020 09:14:04: 27000000 INFO @ Tue, 16 Jun 2020 09:14:06: 23000000 INFO @ Tue, 16 Jun 2020 09:14:12: 28000000 INFO @ Tue, 16 Jun 2020 09:14:14: 24000000 INFO @ Tue, 16 Jun 2020 09:14:19: 29000000 INFO @ Tue, 16 Jun 2020 09:14:21: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 09:14:21: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 09:14:21: #1 total tags in treatment: 29176986 INFO @ Tue, 16 Jun 2020 09:14:21: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:14:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:14:21: #1 tags after filtering in treatment: 29176986 INFO @ Tue, 16 Jun 2020 09:14:21: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:14:21: #1 finished! INFO @ Tue, 16 Jun 2020 09:14:21: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:14:21: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:14:22: 25000000 INFO @ Tue, 16 Jun 2020 09:14:23: #2 number of paired peaks: 73 WARNING @ Tue, 16 Jun 2020 09:14:23: Too few paired peaks (73) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:14:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3583347/SRX3583347.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3583347/SRX3583347.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3583347/SRX3583347.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3583347/SRX3583347.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:14:28: 26000000 INFO @ Tue, 16 Jun 2020 09:14:36: 27000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 09:14:43: 28000000 INFO @ Tue, 16 Jun 2020 09:14:50: 29000000 INFO @ Tue, 16 Jun 2020 09:14:52: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 09:14:52: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 09:14:52: #1 total tags in treatment: 29176986 INFO @ Tue, 16 Jun 2020 09:14:52: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:14:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:14:52: #1 tags after filtering in treatment: 29176986 INFO @ Tue, 16 Jun 2020 09:14:52: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:14:52: #1 finished! INFO @ Tue, 16 Jun 2020 09:14:52: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:14:52: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:14:54: #2 number of paired peaks: 73 WARNING @ Tue, 16 Jun 2020 09:14:54: Too few paired peaks (73) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:14:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3583347/SRX3583347.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3583347/SRX3583347.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3583347/SRX3583347.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3583347/SRX3583347.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling