Job ID = 6367592 SRX = SRX331349 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:30:05 prefetch.2.10.7: 1) Downloading 'SRR947586'... 2020-06-15T23:30:05 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:37:47 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:37:47 prefetch.2.10.7: 1) 'SRR947586' was downloaded successfully Read 51067685 spots for SRR947586/SRR947586.sra Written 51067685 spots for SRR947586/SRR947586.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:46 51067685 reads; of these: 51067685 (100.00%) were unpaired; of these: 4585341 (8.98%) aligned 0 times 37934770 (74.28%) aligned exactly 1 time 8547574 (16.74%) aligned >1 times 91.02% overall alignment rate Time searching: 00:10:46 Overall time: 00:10:46 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 9745843 / 46482344 = 0.2097 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:01:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX331349/SRX331349.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX331349/SRX331349.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX331349/SRX331349.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX331349/SRX331349.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:01:46: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:01:46: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:01:52: 1000000 INFO @ Tue, 16 Jun 2020 09:01:58: 2000000 INFO @ Tue, 16 Jun 2020 09:02:04: 3000000 INFO @ Tue, 16 Jun 2020 09:02:10: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:02:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX331349/SRX331349.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX331349/SRX331349.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX331349/SRX331349.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX331349/SRX331349.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:02:16: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:02:16: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:02:17: 5000000 INFO @ Tue, 16 Jun 2020 09:02:23: 1000000 INFO @ Tue, 16 Jun 2020 09:02:23: 6000000 INFO @ Tue, 16 Jun 2020 09:02:29: 2000000 INFO @ Tue, 16 Jun 2020 09:02:30: 7000000 INFO @ Tue, 16 Jun 2020 09:02:36: 3000000 INFO @ Tue, 16 Jun 2020 09:02:36: 8000000 INFO @ Tue, 16 Jun 2020 09:02:42: 4000000 INFO @ Tue, 16 Jun 2020 09:02:43: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:02:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX331349/SRX331349.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX331349/SRX331349.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX331349/SRX331349.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX331349/SRX331349.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:02:46: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:02:46: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:02:49: 5000000 INFO @ Tue, 16 Jun 2020 09:02:49: 10000000 INFO @ Tue, 16 Jun 2020 09:02:53: 1000000 INFO @ Tue, 16 Jun 2020 09:02:55: 6000000 INFO @ Tue, 16 Jun 2020 09:02:56: 11000000 INFO @ Tue, 16 Jun 2020 09:02:59: 2000000 INFO @ Tue, 16 Jun 2020 09:03:02: 7000000 INFO @ Tue, 16 Jun 2020 09:03:02: 12000000 INFO @ Tue, 16 Jun 2020 09:03:06: 3000000 INFO @ Tue, 16 Jun 2020 09:03:08: 8000000 INFO @ Tue, 16 Jun 2020 09:03:09: 13000000 INFO @ Tue, 16 Jun 2020 09:03:13: 4000000 INFO @ Tue, 16 Jun 2020 09:03:15: 9000000 INFO @ Tue, 16 Jun 2020 09:03:15: 14000000 INFO @ Tue, 16 Jun 2020 09:03:20: 5000000 INFO @ Tue, 16 Jun 2020 09:03:21: 10000000 INFO @ Tue, 16 Jun 2020 09:03:22: 15000000 INFO @ Tue, 16 Jun 2020 09:03:26: 6000000 INFO @ Tue, 16 Jun 2020 09:03:27: 11000000 INFO @ Tue, 16 Jun 2020 09:03:29: 16000000 INFO @ Tue, 16 Jun 2020 09:03:33: 7000000 INFO @ Tue, 16 Jun 2020 09:03:34: 12000000 INFO @ Tue, 16 Jun 2020 09:03:36: 17000000 INFO @ Tue, 16 Jun 2020 09:03:39: 8000000 INFO @ Tue, 16 Jun 2020 09:03:40: 13000000 INFO @ Tue, 16 Jun 2020 09:03:42: 18000000 INFO @ Tue, 16 Jun 2020 09:03:46: 9000000 INFO @ Tue, 16 Jun 2020 09:03:46: 14000000 INFO @ Tue, 16 Jun 2020 09:03:49: 19000000 INFO @ Tue, 16 Jun 2020 09:03:52: 10000000 INFO @ Tue, 16 Jun 2020 09:03:52: 15000000 INFO @ Tue, 16 Jun 2020 09:03:55: 20000000 INFO @ Tue, 16 Jun 2020 09:03:59: 11000000 INFO @ Tue, 16 Jun 2020 09:03:59: 16000000 INFO @ Tue, 16 Jun 2020 09:04:01: 21000000 INFO @ Tue, 16 Jun 2020 09:04:05: 12000000 INFO @ Tue, 16 Jun 2020 09:04:05: 17000000 INFO @ Tue, 16 Jun 2020 09:04:08: 22000000 INFO @ Tue, 16 Jun 2020 09:04:11: 18000000 INFO @ Tue, 16 Jun 2020 09:04:11: 13000000 INFO @ Tue, 16 Jun 2020 09:04:14: 23000000 INFO @ Tue, 16 Jun 2020 09:04:18: 19000000 INFO @ Tue, 16 Jun 2020 09:04:18: 14000000 INFO @ Tue, 16 Jun 2020 09:04:21: 24000000 INFO @ Tue, 16 Jun 2020 09:04:24: 20000000 INFO @ Tue, 16 Jun 2020 09:04:25: 15000000 INFO @ Tue, 16 Jun 2020 09:04:27: 25000000 INFO @ Tue, 16 Jun 2020 09:04:30: 21000000 INFO @ Tue, 16 Jun 2020 09:04:31: 16000000 INFO @ Tue, 16 Jun 2020 09:04:34: 26000000 INFO @ Tue, 16 Jun 2020 09:04:36: 22000000 INFO @ Tue, 16 Jun 2020 09:04:38: 17000000 INFO @ Tue, 16 Jun 2020 09:04:41: 27000000 INFO @ Tue, 16 Jun 2020 09:04:43: 23000000 INFO @ Tue, 16 Jun 2020 09:04:44: 18000000 INFO @ Tue, 16 Jun 2020 09:04:48: 28000000 INFO @ Tue, 16 Jun 2020 09:04:49: 24000000 INFO @ Tue, 16 Jun 2020 09:04:51: 19000000 INFO @ Tue, 16 Jun 2020 09:04:54: 29000000 INFO @ Tue, 16 Jun 2020 09:04:55: 25000000 INFO @ Tue, 16 Jun 2020 09:04:58: 20000000 INFO @ Tue, 16 Jun 2020 09:05:01: 30000000 INFO @ Tue, 16 Jun 2020 09:05:02: 26000000 INFO @ Tue, 16 Jun 2020 09:05:04: 21000000 INFO @ Tue, 16 Jun 2020 09:05:07: 31000000 INFO @ Tue, 16 Jun 2020 09:05:09: 27000000 INFO @ Tue, 16 Jun 2020 09:05:11: 22000000 INFO @ Tue, 16 Jun 2020 09:05:14: 32000000 INFO @ Tue, 16 Jun 2020 09:05:15: 28000000 INFO @ Tue, 16 Jun 2020 09:05:17: 23000000 INFO @ Tue, 16 Jun 2020 09:05:20: 33000000 INFO @ Tue, 16 Jun 2020 09:05:22: 29000000 INFO @ Tue, 16 Jun 2020 09:05:24: 24000000 INFO @ Tue, 16 Jun 2020 09:05:27: 34000000 INFO @ Tue, 16 Jun 2020 09:05:28: 30000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:05:30: 25000000 INFO @ Tue, 16 Jun 2020 09:05:33: 35000000 INFO @ Tue, 16 Jun 2020 09:05:34: 31000000 INFO @ Tue, 16 Jun 2020 09:05:37: 26000000 INFO @ Tue, 16 Jun 2020 09:05:40: 36000000 INFO @ Tue, 16 Jun 2020 09:05:41: 32000000 INFO @ Tue, 16 Jun 2020 09:05:43: 27000000 INFO @ Tue, 16 Jun 2020 09:05:45: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:05:45: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:05:45: #1 total tags in treatment: 36736501 INFO @ Tue, 16 Jun 2020 09:05:45: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:05:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:05:45: #1 tags after filtering in treatment: 36736501 INFO @ Tue, 16 Jun 2020 09:05:45: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:05:45: #1 finished! INFO @ Tue, 16 Jun 2020 09:05:45: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:05:45: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:05:47: 33000000 INFO @ Tue, 16 Jun 2020 09:05:48: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:05:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:05:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX331349/SRX331349.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX331349/SRX331349.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX331349/SRX331349.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX331349/SRX331349.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:05:50: 28000000 INFO @ Tue, 16 Jun 2020 09:05:53: 34000000 INFO @ Tue, 16 Jun 2020 09:05:56: 29000000 INFO @ Tue, 16 Jun 2020 09:06:00: 35000000 INFO @ Tue, 16 Jun 2020 09:06:03: 30000000 INFO @ Tue, 16 Jun 2020 09:06:06: 36000000 INFO @ Tue, 16 Jun 2020 09:06:09: 31000000 INFO @ Tue, 16 Jun 2020 09:06:11: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:06:11: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:06:11: #1 total tags in treatment: 36736501 INFO @ Tue, 16 Jun 2020 09:06:11: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:06:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:06:12: #1 tags after filtering in treatment: 36736501 INFO @ Tue, 16 Jun 2020 09:06:12: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:06:12: #1 finished! INFO @ Tue, 16 Jun 2020 09:06:12: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:06:12: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:06:14: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:06:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:06:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX331349/SRX331349.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX331349/SRX331349.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX331349/SRX331349.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX331349/SRX331349.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:06:15: 32000000 INFO @ Tue, 16 Jun 2020 09:06:21: 33000000 INFO @ Tue, 16 Jun 2020 09:06:27: 34000000 INFO @ Tue, 16 Jun 2020 09:06:33: 35000000 INFO @ Tue, 16 Jun 2020 09:06:39: 36000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 09:06:43: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:06:43: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:06:43: #1 total tags in treatment: 36736501 INFO @ Tue, 16 Jun 2020 09:06:43: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:06:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:06:44: #1 tags after filtering in treatment: 36736501 INFO @ Tue, 16 Jun 2020 09:06:44: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:06:44: #1 finished! INFO @ Tue, 16 Jun 2020 09:06:44: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:06:44: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:06:46: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:06:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:06:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX331349/SRX331349.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX331349/SRX331349.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX331349/SRX331349.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX331349/SRX331349.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling