Job ID = 6367372 SRX = SRX331131 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:35:59 prefetch.2.10.7: 1) Downloading 'SRR947362'... 2020-06-15T23:35:59 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:36:55 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:36:55 prefetch.2.10.7: 'SRR947362' is valid 2020-06-15T23:36:55 prefetch.2.10.7: 1) 'SRR947362' was downloaded successfully Read 7588024 spots for SRR947362/SRR947362.sra Written 7588024 spots for SRR947362/SRR947362.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:16 7588024 reads; of these: 7588024 (100.00%) were unpaired; of these: 81294 (1.07%) aligned 0 times 6336011 (83.50%) aligned exactly 1 time 1170719 (15.43%) aligned >1 times 98.93% overall alignment rate Time searching: 00:01:16 Overall time: 00:01:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 628810 / 7506730 = 0.0838 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:40:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX331131/SRX331131.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX331131/SRX331131.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX331131/SRX331131.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX331131/SRX331131.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:40:39: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:40:39: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:40:45: 1000000 INFO @ Tue, 16 Jun 2020 08:40:52: 2000000 INFO @ Tue, 16 Jun 2020 08:40:58: 3000000 INFO @ Tue, 16 Jun 2020 08:41:05: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:41:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX331131/SRX331131.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX331131/SRX331131.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX331131/SRX331131.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX331131/SRX331131.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:41:09: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:41:09: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:41:11: 5000000 INFO @ Tue, 16 Jun 2020 08:41:16: 1000000 INFO @ Tue, 16 Jun 2020 08:41:18: 6000000 INFO @ Tue, 16 Jun 2020 08:41:23: 2000000 INFO @ Tue, 16 Jun 2020 08:41:24: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 08:41:24: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 08:41:24: #1 total tags in treatment: 6877920 INFO @ Tue, 16 Jun 2020 08:41:24: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:41:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:41:24: #1 tags after filtering in treatment: 6877920 INFO @ Tue, 16 Jun 2020 08:41:24: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:41:24: #1 finished! INFO @ Tue, 16 Jun 2020 08:41:24: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:41:24: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:41:25: #2 number of paired peaks: 430 WARNING @ Tue, 16 Jun 2020 08:41:25: Fewer paired peaks (430) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 430 pairs to build model! INFO @ Tue, 16 Jun 2020 08:41:25: start model_add_line... INFO @ Tue, 16 Jun 2020 08:41:25: start X-correlation... INFO @ Tue, 16 Jun 2020 08:41:25: end of X-cor INFO @ Tue, 16 Jun 2020 08:41:25: #2 finished! INFO @ Tue, 16 Jun 2020 08:41:25: #2 predicted fragment length is 30 bps INFO @ Tue, 16 Jun 2020 08:41:25: #2 alternative fragment length(s) may be 3,30,544,590 bps INFO @ Tue, 16 Jun 2020 08:41:25: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX331131/SRX331131.05_model.r WARNING @ Tue, 16 Jun 2020 08:41:25: #2 Since the d (30) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 08:41:25: #2 You may need to consider one of the other alternative d(s): 3,30,544,590 WARNING @ Tue, 16 Jun 2020 08:41:25: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 08:41:25: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:41:25: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 08:41:29: 3000000 INFO @ Tue, 16 Jun 2020 08:41:36: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:41:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX331131/SRX331131.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX331131/SRX331131.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX331131/SRX331131.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX331131/SRX331131.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:41:39: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:41:39: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:41:39: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 08:41:43: 5000000 INFO @ Tue, 16 Jun 2020 08:41:46: 1000000 INFO @ Tue, 16 Jun 2020 08:41:46: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX331131/SRX331131.05_peaks.xls INFO @ Tue, 16 Jun 2020 08:41:46: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX331131/SRX331131.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:41:46: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX331131/SRX331131.05_summits.bed INFO @ Tue, 16 Jun 2020 08:41:46: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (505 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:41:50: 6000000 INFO @ Tue, 16 Jun 2020 08:41:53: 2000000 INFO @ Tue, 16 Jun 2020 08:41:56: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 08:41:56: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 08:41:56: #1 total tags in treatment: 6877920 INFO @ Tue, 16 Jun 2020 08:41:56: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:41:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:41:56: #1 tags after filtering in treatment: 6877920 INFO @ Tue, 16 Jun 2020 08:41:56: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:41:56: #1 finished! INFO @ Tue, 16 Jun 2020 08:41:56: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:41:56: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:41:56: #2 number of paired peaks: 430 WARNING @ Tue, 16 Jun 2020 08:41:56: Fewer paired peaks (430) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 430 pairs to build model! INFO @ Tue, 16 Jun 2020 08:41:56: start model_add_line... INFO @ Tue, 16 Jun 2020 08:41:57: start X-correlation... INFO @ Tue, 16 Jun 2020 08:41:57: end of X-cor INFO @ Tue, 16 Jun 2020 08:41:57: #2 finished! INFO @ Tue, 16 Jun 2020 08:41:57: #2 predicted fragment length is 30 bps INFO @ Tue, 16 Jun 2020 08:41:57: #2 alternative fragment length(s) may be 3,30,544,590 bps INFO @ Tue, 16 Jun 2020 08:41:57: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX331131/SRX331131.10_model.r WARNING @ Tue, 16 Jun 2020 08:41:57: #2 Since the d (30) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 08:41:57: #2 You may need to consider one of the other alternative d(s): 3,30,544,590 WARNING @ Tue, 16 Jun 2020 08:41:57: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 08:41:57: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:41:57: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 08:42:00: 3000000 INFO @ Tue, 16 Jun 2020 08:42:07: 4000000 INFO @ Tue, 16 Jun 2020 08:42:11: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 08:42:13: 5000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 08:42:18: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX331131/SRX331131.10_peaks.xls INFO @ Tue, 16 Jun 2020 08:42:18: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX331131/SRX331131.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:42:18: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX331131/SRX331131.10_summits.bed INFO @ Tue, 16 Jun 2020 08:42:18: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (259 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:42:20: 6000000 INFO @ Tue, 16 Jun 2020 08:42:26: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 08:42:26: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 08:42:26: #1 total tags in treatment: 6877920 INFO @ Tue, 16 Jun 2020 08:42:26: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:42:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:42:26: #1 tags after filtering in treatment: 6877920 INFO @ Tue, 16 Jun 2020 08:42:26: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:42:26: #1 finished! INFO @ Tue, 16 Jun 2020 08:42:26: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:42:26: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:42:26: #2 number of paired peaks: 430 WARNING @ Tue, 16 Jun 2020 08:42:26: Fewer paired peaks (430) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 430 pairs to build model! INFO @ Tue, 16 Jun 2020 08:42:26: start model_add_line... INFO @ Tue, 16 Jun 2020 08:42:26: start X-correlation... INFO @ Tue, 16 Jun 2020 08:42:26: end of X-cor INFO @ Tue, 16 Jun 2020 08:42:26: #2 finished! INFO @ Tue, 16 Jun 2020 08:42:26: #2 predicted fragment length is 30 bps INFO @ Tue, 16 Jun 2020 08:42:26: #2 alternative fragment length(s) may be 3,30,544,590 bps INFO @ Tue, 16 Jun 2020 08:42:26: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX331131/SRX331131.20_model.r WARNING @ Tue, 16 Jun 2020 08:42:26: #2 Since the d (30) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 08:42:26: #2 You may need to consider one of the other alternative d(s): 3,30,544,590 WARNING @ Tue, 16 Jun 2020 08:42:26: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 08:42:26: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:42:26: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 08:42:41: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 08:42:48: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX331131/SRX331131.20_peaks.xls INFO @ Tue, 16 Jun 2020 08:42:48: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX331131/SRX331131.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:42:48: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX331131/SRX331131.20_summits.bed INFO @ Tue, 16 Jun 2020 08:42:48: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (65 records, 4 fields): 2 millis CompletedMACS2peakCalling