Job ID = 6367264 SRX = SRX323685 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:24:16 prefetch.2.10.7: 1) Downloading 'SRR935759'... 2020-06-15T23:24:16 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:25:30 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:25:30 prefetch.2.10.7: 1) 'SRR935759' was downloaded successfully Read 17531086 spots for SRR935759/SRR935759.sra Written 17531086 spots for SRR935759/SRR935759.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:07 17531086 reads; of these: 17531086 (100.00%) were unpaired; of these: 1542786 (8.80%) aligned 0 times 14049027 (80.14%) aligned exactly 1 time 1939273 (11.06%) aligned >1 times 91.20% overall alignment rate Time searching: 00:05:07 Overall time: 00:05:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4077697 / 15988300 = 0.2550 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:36:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX323685/SRX323685.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX323685/SRX323685.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX323685/SRX323685.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX323685/SRX323685.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:36:33: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:36:33: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:36:41: 1000000 INFO @ Tue, 16 Jun 2020 08:36:49: 2000000 INFO @ Tue, 16 Jun 2020 08:36:56: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:37:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX323685/SRX323685.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX323685/SRX323685.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX323685/SRX323685.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX323685/SRX323685.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:37:03: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:37:03: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:37:04: 4000000 INFO @ Tue, 16 Jun 2020 08:37:11: 1000000 INFO @ Tue, 16 Jun 2020 08:37:13: 5000000 INFO @ Tue, 16 Jun 2020 08:37:19: 2000000 INFO @ Tue, 16 Jun 2020 08:37:21: 6000000 INFO @ Tue, 16 Jun 2020 08:37:27: 3000000 INFO @ Tue, 16 Jun 2020 08:37:29: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:37:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX323685/SRX323685.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX323685/SRX323685.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX323685/SRX323685.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX323685/SRX323685.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:37:33: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:37:33: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:37:35: 4000000 INFO @ Tue, 16 Jun 2020 08:37:38: 8000000 INFO @ Tue, 16 Jun 2020 08:37:41: 1000000 INFO @ Tue, 16 Jun 2020 08:37:43: 5000000 INFO @ Tue, 16 Jun 2020 08:37:46: 9000000 INFO @ Tue, 16 Jun 2020 08:37:49: 2000000 INFO @ Tue, 16 Jun 2020 08:37:51: 6000000 INFO @ Tue, 16 Jun 2020 08:37:54: 10000000 INFO @ Tue, 16 Jun 2020 08:37:57: 3000000 INFO @ Tue, 16 Jun 2020 08:37:59: 7000000 INFO @ Tue, 16 Jun 2020 08:38:02: 11000000 INFO @ Tue, 16 Jun 2020 08:38:04: 4000000 INFO @ Tue, 16 Jun 2020 08:38:06: 8000000 INFO @ Tue, 16 Jun 2020 08:38:10: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 08:38:10: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 08:38:10: #1 total tags in treatment: 11910603 INFO @ Tue, 16 Jun 2020 08:38:10: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:38:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:38:10: #1 tags after filtering in treatment: 11910603 INFO @ Tue, 16 Jun 2020 08:38:10: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:38:10: #1 finished! INFO @ Tue, 16 Jun 2020 08:38:10: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:38:10: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:38:11: #2 number of paired peaks: 47 WARNING @ Tue, 16 Jun 2020 08:38:11: Too few paired peaks (47) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:38:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX323685/SRX323685.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX323685/SRX323685.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX323685/SRX323685.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX323685/SRX323685.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:38:12: 5000000 INFO @ Tue, 16 Jun 2020 08:38:14: 9000000 INFO @ Tue, 16 Jun 2020 08:38:19: 6000000 INFO @ Tue, 16 Jun 2020 08:38:21: 10000000 INFO @ Tue, 16 Jun 2020 08:38:27: 7000000 INFO @ Tue, 16 Jun 2020 08:38:29: 11000000 INFO @ Tue, 16 Jun 2020 08:38:34: 8000000 INFO @ Tue, 16 Jun 2020 08:38:36: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 08:38:36: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 08:38:36: #1 total tags in treatment: 11910603 INFO @ Tue, 16 Jun 2020 08:38:36: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:38:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:38:36: #1 tags after filtering in treatment: 11910603 INFO @ Tue, 16 Jun 2020 08:38:36: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:38:36: #1 finished! INFO @ Tue, 16 Jun 2020 08:38:36: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:38:36: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:38:37: #2 number of paired peaks: 47 WARNING @ Tue, 16 Jun 2020 08:38:37: Too few paired peaks (47) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:38:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX323685/SRX323685.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX323685/SRX323685.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX323685/SRX323685.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX323685/SRX323685.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:38:41: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 08:38:47: 10000000 INFO @ Tue, 16 Jun 2020 08:38:54: 11000000 INFO @ Tue, 16 Jun 2020 08:39:00: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 08:39:00: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 08:39:00: #1 total tags in treatment: 11910603 INFO @ Tue, 16 Jun 2020 08:39:00: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:39:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:39:00: #1 tags after filtering in treatment: 11910603 INFO @ Tue, 16 Jun 2020 08:39:00: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:39:00: #1 finished! INFO @ Tue, 16 Jun 2020 08:39:00: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:39:00: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:39:01: #2 number of paired peaks: 47 WARNING @ Tue, 16 Jun 2020 08:39:01: Too few paired peaks (47) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:39:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX323685/SRX323685.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX323685/SRX323685.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX323685/SRX323685.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX323685/SRX323685.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。