Job ID = 12265582 SRX = SRX3029131 Genome = ce11 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:47:28 50527041 reads; of these: 50527041 (100.00%) were paired; of these: 19028402 (37.66%) aligned concordantly 0 times 27872713 (55.16%) aligned concordantly exactly 1 time 3625926 (7.18%) aligned concordantly >1 times ---- 19028402 pairs aligned concordantly 0 times; of these: 4778100 (25.11%) aligned discordantly 1 time ---- 14250302 pairs aligned 0 times concordantly or discordantly; of these: 28500604 mates make up the pairs; of these: 26264453 (92.15%) aligned 0 times 1046754 (3.67%) aligned exactly 1 time 1189397 (4.17%) aligned >1 times 74.01% overall alignment rate Time searching: 00:47:29 Overall time: 00:47:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 32 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 10110876 / 35908296 = 0.2816 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:57:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3029131/SRX3029131.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3029131/SRX3029131.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3029131/SRX3029131.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3029131/SRX3029131.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:57:23: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:57:23: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:57:31: 1000000 INFO @ Sat, 03 Apr 2021 08:57:39: 2000000 INFO @ Sat, 03 Apr 2021 08:57:47: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:57:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3029131/SRX3029131.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3029131/SRX3029131.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3029131/SRX3029131.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3029131/SRX3029131.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:57:53: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:57:53: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:57:56: 4000000 INFO @ Sat, 03 Apr 2021 08:58:02: 1000000 INFO @ Sat, 03 Apr 2021 08:58:05: 5000000 INFO @ Sat, 03 Apr 2021 08:58:10: 2000000 INFO @ Sat, 03 Apr 2021 08:58:14: 6000000 INFO @ Sat, 03 Apr 2021 08:58:18: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:58:23: 7000000 INFO @ Sat, 03 Apr 2021 08:58:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3029131/SRX3029131.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3029131/SRX3029131.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3029131/SRX3029131.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3029131/SRX3029131.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:58:23: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:58:23: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:58:27: 4000000 INFO @ Sat, 03 Apr 2021 08:58:32: 1000000 INFO @ Sat, 03 Apr 2021 08:58:32: 8000000 INFO @ Sat, 03 Apr 2021 08:58:35: 5000000 INFO @ Sat, 03 Apr 2021 08:58:41: 9000000 INFO @ Sat, 03 Apr 2021 08:58:41: 2000000 INFO @ Sat, 03 Apr 2021 08:58:44: 6000000 INFO @ Sat, 03 Apr 2021 08:58:50: 10000000 INFO @ Sat, 03 Apr 2021 08:58:50: 3000000 INFO @ Sat, 03 Apr 2021 08:58:52: 7000000 INFO @ Sat, 03 Apr 2021 08:58:59: 11000000 INFO @ Sat, 03 Apr 2021 08:58:59: 4000000 INFO @ Sat, 03 Apr 2021 08:59:00: 8000000 INFO @ Sat, 03 Apr 2021 08:59:08: 12000000 INFO @ Sat, 03 Apr 2021 08:59:09: 5000000 INFO @ Sat, 03 Apr 2021 08:59:09: 9000000 INFO @ Sat, 03 Apr 2021 08:59:17: 13000000 INFO @ Sat, 03 Apr 2021 08:59:17: 10000000 INFO @ Sat, 03 Apr 2021 08:59:18: 6000000 INFO @ Sat, 03 Apr 2021 08:59:25: 11000000 INFO @ Sat, 03 Apr 2021 08:59:26: 14000000 INFO @ Sat, 03 Apr 2021 08:59:27: 7000000 INFO @ Sat, 03 Apr 2021 08:59:34: 12000000 INFO @ Sat, 03 Apr 2021 08:59:35: 15000000 INFO @ Sat, 03 Apr 2021 08:59:37: 8000000 INFO @ Sat, 03 Apr 2021 08:59:42: 13000000 INFO @ Sat, 03 Apr 2021 08:59:44: 16000000 INFO @ Sat, 03 Apr 2021 08:59:46: 9000000 INFO @ Sat, 03 Apr 2021 08:59:51: 14000000 INFO @ Sat, 03 Apr 2021 08:59:53: 17000000 INFO @ Sat, 03 Apr 2021 08:59:55: 10000000 INFO @ Sat, 03 Apr 2021 08:59:59: 15000000 INFO @ Sat, 03 Apr 2021 09:00:03: 18000000 INFO @ Sat, 03 Apr 2021 09:00:04: 11000000 INFO @ Sat, 03 Apr 2021 09:00:07: 16000000 INFO @ Sat, 03 Apr 2021 09:00:12: 19000000 INFO @ Sat, 03 Apr 2021 09:00:14: 12000000 INFO @ Sat, 03 Apr 2021 09:00:16: 17000000 INFO @ Sat, 03 Apr 2021 09:00:21: 20000000 INFO @ Sat, 03 Apr 2021 09:00:23: 13000000 INFO @ Sat, 03 Apr 2021 09:00:24: 18000000 INFO @ Sat, 03 Apr 2021 09:00:30: 21000000 INFO @ Sat, 03 Apr 2021 09:00:32: 19000000 INFO @ Sat, 03 Apr 2021 09:00:33: 14000000 INFO @ Sat, 03 Apr 2021 09:00:39: 22000000 INFO @ Sat, 03 Apr 2021 09:00:41: 20000000 INFO @ Sat, 03 Apr 2021 09:00:42: 15000000 INFO @ Sat, 03 Apr 2021 09:00:48: 23000000 INFO @ Sat, 03 Apr 2021 09:00:49: 21000000 INFO @ Sat, 03 Apr 2021 09:00:51: 16000000 INFO @ Sat, 03 Apr 2021 09:00:57: 24000000 INFO @ Sat, 03 Apr 2021 09:00:57: 22000000 INFO @ Sat, 03 Apr 2021 09:01:00: 17000000 INFO @ Sat, 03 Apr 2021 09:01:06: 23000000 INFO @ Sat, 03 Apr 2021 09:01:06: 25000000 INFO @ Sat, 03 Apr 2021 09:01:10: 18000000 INFO @ Sat, 03 Apr 2021 09:01:14: 24000000 INFO @ Sat, 03 Apr 2021 09:01:15: 26000000 INFO @ Sat, 03 Apr 2021 09:01:19: 19000000 INFO @ Sat, 03 Apr 2021 09:01:22: 25000000 INFO @ Sat, 03 Apr 2021 09:01:24: 27000000 INFO @ Sat, 03 Apr 2021 09:01:28: 20000000 INFO @ Sat, 03 Apr 2021 09:01:31: 26000000 INFO @ Sat, 03 Apr 2021 09:01:33: 28000000 INFO @ Sat, 03 Apr 2021 09:01:38: 21000000 INFO @ Sat, 03 Apr 2021 09:01:39: 27000000 INFO @ Sat, 03 Apr 2021 09:01:42: 29000000 INFO @ Sat, 03 Apr 2021 09:01:47: 22000000 INFO @ Sat, 03 Apr 2021 09:01:48: 28000000 INFO @ Sat, 03 Apr 2021 09:01:50: 30000000 INFO @ Sat, 03 Apr 2021 09:01:56: 29000000 INFO @ Sat, 03 Apr 2021 09:01:56: 23000000 INFO @ Sat, 03 Apr 2021 09:01:59: 31000000 INFO @ Sat, 03 Apr 2021 09:02:04: 30000000 INFO @ Sat, 03 Apr 2021 09:02:06: 24000000 INFO @ Sat, 03 Apr 2021 09:02:08: 32000000 INFO @ Sat, 03 Apr 2021 09:02:13: 31000000 INFO @ Sat, 03 Apr 2021 09:02:15: 25000000 INFO @ Sat, 03 Apr 2021 09:02:17: 33000000 INFO @ Sat, 03 Apr 2021 09:02:21: 32000000 INFO @ Sat, 03 Apr 2021 09:02:25: 26000000 INFO @ Sat, 03 Apr 2021 09:02:26: 34000000 INFO @ Sat, 03 Apr 2021 09:02:29: 33000000 INFO @ Sat, 03 Apr 2021 09:02:34: 27000000 INFO @ Sat, 03 Apr 2021 09:02:35: 35000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 09:02:37: 34000000 INFO @ Sat, 03 Apr 2021 09:02:44: 28000000 INFO @ Sat, 03 Apr 2021 09:02:44: 36000000 INFO @ Sat, 03 Apr 2021 09:02:46: 35000000 INFO @ Sat, 03 Apr 2021 09:02:53: 37000000 INFO @ Sat, 03 Apr 2021 09:02:53: 29000000 INFO @ Sat, 03 Apr 2021 09:02:54: 36000000 INFO @ Sat, 03 Apr 2021 09:03:01: 38000000 INFO @ Sat, 03 Apr 2021 09:03:02: 37000000 INFO @ Sat, 03 Apr 2021 09:03:02: 30000000 INFO @ Sat, 03 Apr 2021 09:03:10: 39000000 INFO @ Sat, 03 Apr 2021 09:03:11: 38000000 INFO @ Sat, 03 Apr 2021 09:03:12: 31000000 INFO @ Sat, 03 Apr 2021 09:03:18: 40000000 INFO @ Sat, 03 Apr 2021 09:03:19: 39000000 INFO @ Sat, 03 Apr 2021 09:03:21: 32000000 INFO @ Sat, 03 Apr 2021 09:03:27: 40000000 INFO @ Sat, 03 Apr 2021 09:03:27: 41000000 INFO @ Sat, 03 Apr 2021 09:03:30: 33000000 INFO @ Sat, 03 Apr 2021 09:03:35: 41000000 INFO @ Sat, 03 Apr 2021 09:03:36: 42000000 INFO @ Sat, 03 Apr 2021 09:03:40: 34000000 INFO @ Sat, 03 Apr 2021 09:03:44: 42000000 INFO @ Sat, 03 Apr 2021 09:03:45: 43000000 INFO @ Sat, 03 Apr 2021 09:03:49: 35000000 INFO @ Sat, 03 Apr 2021 09:03:52: 43000000 BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 09:03:54: 44000000 INFO @ Sat, 03 Apr 2021 09:03:59: 36000000 INFO @ Sat, 03 Apr 2021 09:04:00: 44000000 INFO @ Sat, 03 Apr 2021 09:04:03: 45000000 INFO @ Sat, 03 Apr 2021 09:04:08: 37000000 INFO @ Sat, 03 Apr 2021 09:04:09: 45000000 INFO @ Sat, 03 Apr 2021 09:04:12: 46000000 INFO @ Sat, 03 Apr 2021 09:04:17: 38000000 INFO @ Sat, 03 Apr 2021 09:04:17: 46000000 INFO @ Sat, 03 Apr 2021 09:04:22: 47000000 INFO @ Sat, 03 Apr 2021 09:04:26: 47000000 INFO @ Sat, 03 Apr 2021 09:04:26: 39000000 INFO @ Sat, 03 Apr 2021 09:04:31: 48000000 INFO @ Sat, 03 Apr 2021 09:04:34: 48000000 INFO @ Sat, 03 Apr 2021 09:04:36: 40000000 INFO @ Sat, 03 Apr 2021 09:04:40: 49000000 INFO @ Sat, 03 Apr 2021 09:04:42: 49000000 INFO @ Sat, 03 Apr 2021 09:04:45: 41000000 INFO @ Sat, 03 Apr 2021 09:04:48: 50000000 INFO @ Sat, 03 Apr 2021 09:04:50: 50000000 INFO @ Sat, 03 Apr 2021 09:04:54: 42000000 INFO @ Sat, 03 Apr 2021 09:04:57: 51000000 INFO @ Sat, 03 Apr 2021 09:04:59: 51000000 INFO @ Sat, 03 Apr 2021 09:05:03: 43000000 INFO @ Sat, 03 Apr 2021 09:05:06: 52000000 INFO @ Sat, 03 Apr 2021 09:05:07: 52000000 INFO @ Sat, 03 Apr 2021 09:05:13: 44000000 INFO @ Sat, 03 Apr 2021 09:05:15: 53000000 INFO @ Sat, 03 Apr 2021 09:05:16: 53000000 INFO @ Sat, 03 Apr 2021 09:05:23: 45000000 INFO @ Sat, 03 Apr 2021 09:05:24: 54000000 INFO @ Sat, 03 Apr 2021 09:05:25: 54000000 INFO @ Sat, 03 Apr 2021 09:05:30: #1 tag size is determined as 76 bps INFO @ Sat, 03 Apr 2021 09:05:30: #1 tag size = 76 INFO @ Sat, 03 Apr 2021 09:05:30: #1 total tags in treatment: 22728562 INFO @ Sat, 03 Apr 2021 09:05:30: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 09:05:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 09:05:30: #1 tag size is determined as 76 bps INFO @ Sat, 03 Apr 2021 09:05:30: #1 tag size = 76 INFO @ Sat, 03 Apr 2021 09:05:30: #1 total tags in treatment: 22728562 INFO @ Sat, 03 Apr 2021 09:05:30: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 09:05:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 09:05:30: #1 tags after filtering in treatment: 16170348 INFO @ Sat, 03 Apr 2021 09:05:30: #1 Redundant rate of treatment: 0.29 INFO @ Sat, 03 Apr 2021 09:05:30: #1 finished! INFO @ Sat, 03 Apr 2021 09:05:30: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 09:05:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 09:05:31: #1 tags after filtering in treatment: 16170348 INFO @ Sat, 03 Apr 2021 09:05:31: #1 Redundant rate of treatment: 0.29 INFO @ Sat, 03 Apr 2021 09:05:31: #1 finished! INFO @ Sat, 03 Apr 2021 09:05:31: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 09:05:31: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 09:05:32: #2 number of paired peaks: 177 WARNING @ Sat, 03 Apr 2021 09:05:32: Fewer paired peaks (177) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 177 pairs to build model! INFO @ Sat, 03 Apr 2021 09:05:32: start model_add_line... INFO @ Sat, 03 Apr 2021 09:05:32: start X-correlation... INFO @ Sat, 03 Apr 2021 09:05:32: end of X-cor INFO @ Sat, 03 Apr 2021 09:05:32: #2 finished! INFO @ Sat, 03 Apr 2021 09:05:32: #2 predicted fragment length is 112 bps INFO @ Sat, 03 Apr 2021 09:05:32: #2 alternative fragment length(s) may be 4,112 bps INFO @ Sat, 03 Apr 2021 09:05:32: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX3029131/SRX3029131.05_model.r WARNING @ Sat, 03 Apr 2021 09:05:32: #2 Since the d (112) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 09:05:32: #2 You may need to consider one of the other alternative d(s): 4,112 WARNING @ Sat, 03 Apr 2021 09:05:32: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 09:05:32: #3 Call peaks... INFO @ Sat, 03 Apr 2021 09:05:32: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 09:05:32: #2 number of paired peaks: 177 WARNING @ Sat, 03 Apr 2021 09:05:32: Fewer paired peaks (177) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 177 pairs to build model! INFO @ Sat, 03 Apr 2021 09:05:32: start model_add_line... INFO @ Sat, 03 Apr 2021 09:05:32: start X-correlation... INFO @ Sat, 03 Apr 2021 09:05:32: 46000000 INFO @ Sat, 03 Apr 2021 09:05:32: end of X-cor INFO @ Sat, 03 Apr 2021 09:05:32: #2 finished! INFO @ Sat, 03 Apr 2021 09:05:32: #2 predicted fragment length is 112 bps INFO @ Sat, 03 Apr 2021 09:05:32: #2 alternative fragment length(s) may be 4,112 bps INFO @ Sat, 03 Apr 2021 09:05:32: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX3029131/SRX3029131.10_model.r WARNING @ Sat, 03 Apr 2021 09:05:32: #2 Since the d (112) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 09:05:32: #2 You may need to consider one of the other alternative d(s): 4,112 WARNING @ Sat, 03 Apr 2021 09:05:32: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 09:05:32: #3 Call peaks... INFO @ Sat, 03 Apr 2021 09:05:32: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 09:05:41: 47000000 INFO @ Sat, 03 Apr 2021 09:05:50: 48000000 INFO @ Sat, 03 Apr 2021 09:05:59: 49000000 INFO @ Sat, 03 Apr 2021 09:06:07: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 09:06:07: 50000000 INFO @ Sat, 03 Apr 2021 09:06:11: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 09:06:15: 51000000 INFO @ Sat, 03 Apr 2021 09:06:23: 52000000 INFO @ Sat, 03 Apr 2021 09:06:26: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX3029131/SRX3029131.05_peaks.xls INFO @ Sat, 03 Apr 2021 09:06:26: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX3029131/SRX3029131.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 09:06:26: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX3029131/SRX3029131.05_summits.bed INFO @ Sat, 03 Apr 2021 09:06:26: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (6578 records, 4 fields): 10 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 09:06:31: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX3029131/SRX3029131.10_peaks.xls INFO @ Sat, 03 Apr 2021 09:06:32: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX3029131/SRX3029131.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 09:06:32: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX3029131/SRX3029131.10_summits.bed INFO @ Sat, 03 Apr 2021 09:06:32: Done! INFO @ Sat, 03 Apr 2021 09:06:32: 53000000 pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (3514 records, 4 fields): 5 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 09:06:40: 54000000 INFO @ Sat, 03 Apr 2021 09:06:44: #1 tag size is determined as 76 bps INFO @ Sat, 03 Apr 2021 09:06:44: #1 tag size = 76 INFO @ Sat, 03 Apr 2021 09:06:44: #1 total tags in treatment: 22728562 INFO @ Sat, 03 Apr 2021 09:06:44: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 09:06:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 09:06:45: #1 tags after filtering in treatment: 16170348 INFO @ Sat, 03 Apr 2021 09:06:45: #1 Redundant rate of treatment: 0.29 INFO @ Sat, 03 Apr 2021 09:06:45: #1 finished! INFO @ Sat, 03 Apr 2021 09:06:45: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 09:06:45: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 09:06:46: #2 number of paired peaks: 177 WARNING @ Sat, 03 Apr 2021 09:06:46: Fewer paired peaks (177) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 177 pairs to build model! INFO @ Sat, 03 Apr 2021 09:06:46: start model_add_line... INFO @ Sat, 03 Apr 2021 09:06:46: start X-correlation... INFO @ Sat, 03 Apr 2021 09:06:46: end of X-cor INFO @ Sat, 03 Apr 2021 09:06:46: #2 finished! INFO @ Sat, 03 Apr 2021 09:06:46: #2 predicted fragment length is 112 bps INFO @ Sat, 03 Apr 2021 09:06:46: #2 alternative fragment length(s) may be 4,112 bps INFO @ Sat, 03 Apr 2021 09:06:46: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX3029131/SRX3029131.20_model.r WARNING @ Sat, 03 Apr 2021 09:06:46: #2 Since the d (112) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 09:06:46: #2 You may need to consider one of the other alternative d(s): 4,112 WARNING @ Sat, 03 Apr 2021 09:06:46: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 09:06:46: #3 Call peaks... INFO @ Sat, 03 Apr 2021 09:06:46: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 09:07:25: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 09:07:43: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX3029131/SRX3029131.20_peaks.xls INFO @ Sat, 03 Apr 2021 09:07:43: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX3029131/SRX3029131.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 09:07:43: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX3029131/SRX3029131.20_summits.bed INFO @ Sat, 03 Apr 2021 09:07:43: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (1392 records, 4 fields): 3 millis CompletedMACS2peakCalling