Job ID = 6626599 SRX = SRX3008771 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:12 44815642 reads; of these: 44815642 (100.00%) were unpaired; of these: 453100 (1.01%) aligned 0 times 37671090 (84.06%) aligned exactly 1 time 6691452 (14.93%) aligned >1 times 98.99% overall alignment rate Time searching: 00:09:12 Overall time: 00:09:12 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8400163 / 44362542 = 0.1894 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:05:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3008771/SRX3008771.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3008771/SRX3008771.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3008771/SRX3008771.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3008771/SRX3008771.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:05:42: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:05:42: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:05:48: 1000000 INFO @ Tue, 14 Jul 2020 08:05:55: 2000000 INFO @ Tue, 14 Jul 2020 08:06:02: 3000000 INFO @ Tue, 14 Jul 2020 08:06:08: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:06:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3008771/SRX3008771.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3008771/SRX3008771.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3008771/SRX3008771.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3008771/SRX3008771.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:06:12: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:06:12: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:06:15: 5000000 INFO @ Tue, 14 Jul 2020 08:06:17: 1000000 INFO @ Tue, 14 Jul 2020 08:06:21: 6000000 INFO @ Tue, 14 Jul 2020 08:06:22: 2000000 INFO @ Tue, 14 Jul 2020 08:06:27: 3000000 INFO @ Tue, 14 Jul 2020 08:06:28: 7000000 INFO @ Tue, 14 Jul 2020 08:06:32: 4000000 INFO @ Tue, 14 Jul 2020 08:06:35: 8000000 INFO @ Tue, 14 Jul 2020 08:06:37: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:06:41: 9000000 INFO @ Tue, 14 Jul 2020 08:06:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3008771/SRX3008771.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3008771/SRX3008771.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3008771/SRX3008771.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3008771/SRX3008771.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:06:41: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:06:41: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:06:42: 6000000 INFO @ Tue, 14 Jul 2020 08:06:47: 7000000 INFO @ Tue, 14 Jul 2020 08:06:47: 1000000 INFO @ Tue, 14 Jul 2020 08:06:48: 10000000 INFO @ Tue, 14 Jul 2020 08:06:52: 2000000 INFO @ Tue, 14 Jul 2020 08:06:52: 8000000 INFO @ Tue, 14 Jul 2020 08:06:55: 11000000 INFO @ Tue, 14 Jul 2020 08:06:58: 9000000 INFO @ Tue, 14 Jul 2020 08:06:58: 3000000 INFO @ Tue, 14 Jul 2020 08:07:01: 12000000 INFO @ Tue, 14 Jul 2020 08:07:03: 10000000 INFO @ Tue, 14 Jul 2020 08:07:03: 4000000 INFO @ Tue, 14 Jul 2020 08:07:08: 11000000 INFO @ Tue, 14 Jul 2020 08:07:08: 5000000 INFO @ Tue, 14 Jul 2020 08:07:08: 13000000 INFO @ Tue, 14 Jul 2020 08:07:13: 12000000 INFO @ Tue, 14 Jul 2020 08:07:13: 6000000 INFO @ Tue, 14 Jul 2020 08:07:15: 14000000 INFO @ Tue, 14 Jul 2020 08:07:19: 13000000 INFO @ Tue, 14 Jul 2020 08:07:19: 7000000 INFO @ Tue, 14 Jul 2020 08:07:22: 15000000 INFO @ Tue, 14 Jul 2020 08:07:24: 14000000 INFO @ Tue, 14 Jul 2020 08:07:24: 8000000 INFO @ Tue, 14 Jul 2020 08:07:29: 16000000 INFO @ Tue, 14 Jul 2020 08:07:29: 15000000 INFO @ Tue, 14 Jul 2020 08:07:29: 9000000 INFO @ Tue, 14 Jul 2020 08:07:34: 16000000 INFO @ Tue, 14 Jul 2020 08:07:34: 10000000 INFO @ Tue, 14 Jul 2020 08:07:35: 17000000 INFO @ Tue, 14 Jul 2020 08:07:39: 17000000 INFO @ Tue, 14 Jul 2020 08:07:39: 11000000 INFO @ Tue, 14 Jul 2020 08:07:41: 18000000 INFO @ Tue, 14 Jul 2020 08:07:44: 18000000 INFO @ Tue, 14 Jul 2020 08:07:44: 12000000 INFO @ Tue, 14 Jul 2020 08:07:47: 19000000 INFO @ Tue, 14 Jul 2020 08:07:49: 19000000 INFO @ Tue, 14 Jul 2020 08:07:49: 13000000 INFO @ Tue, 14 Jul 2020 08:07:54: 20000000 INFO @ Tue, 14 Jul 2020 08:07:55: 14000000 INFO @ Tue, 14 Jul 2020 08:07:55: 20000000 INFO @ Tue, 14 Jul 2020 08:08:00: 15000000 INFO @ Tue, 14 Jul 2020 08:08:00: 21000000 INFO @ Tue, 14 Jul 2020 08:08:00: 21000000 INFO @ Tue, 14 Jul 2020 08:08:05: 16000000 INFO @ Tue, 14 Jul 2020 08:08:06: 22000000 INFO @ Tue, 14 Jul 2020 08:08:06: 22000000 INFO @ Tue, 14 Jul 2020 08:08:10: 17000000 INFO @ Tue, 14 Jul 2020 08:08:11: 23000000 INFO @ Tue, 14 Jul 2020 08:08:13: 23000000 INFO @ Tue, 14 Jul 2020 08:08:15: 18000000 INFO @ Tue, 14 Jul 2020 08:08:16: 24000000 INFO @ Tue, 14 Jul 2020 08:08:19: 24000000 INFO @ Tue, 14 Jul 2020 08:08:20: 19000000 INFO @ Tue, 14 Jul 2020 08:08:21: 25000000 INFO @ Tue, 14 Jul 2020 08:08:25: 20000000 INFO @ Tue, 14 Jul 2020 08:08:26: 25000000 INFO @ Tue, 14 Jul 2020 08:08:26: 26000000 INFO @ Tue, 14 Jul 2020 08:08:30: 21000000 INFO @ Tue, 14 Jul 2020 08:08:31: 27000000 INFO @ Tue, 14 Jul 2020 08:08:32: 26000000 INFO @ Tue, 14 Jul 2020 08:08:35: 22000000 INFO @ Tue, 14 Jul 2020 08:08:36: 28000000 INFO @ Tue, 14 Jul 2020 08:08:39: 27000000 INFO @ Tue, 14 Jul 2020 08:08:40: 23000000 INFO @ Tue, 14 Jul 2020 08:08:41: 29000000 INFO @ Tue, 14 Jul 2020 08:08:45: 28000000 INFO @ Tue, 14 Jul 2020 08:08:45: 24000000 INFO @ Tue, 14 Jul 2020 08:08:46: 30000000 INFO @ Tue, 14 Jul 2020 08:08:51: 25000000 INFO @ Tue, 14 Jul 2020 08:08:51: 29000000 INFO @ Tue, 14 Jul 2020 08:08:52: 31000000 INFO @ Tue, 14 Jul 2020 08:08:56: 26000000 INFO @ Tue, 14 Jul 2020 08:08:57: 32000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 08:08:58: 30000000 INFO @ Tue, 14 Jul 2020 08:09:01: 27000000 INFO @ Tue, 14 Jul 2020 08:09:02: 33000000 INFO @ Tue, 14 Jul 2020 08:09:04: 31000000 INFO @ Tue, 14 Jul 2020 08:09:06: 28000000 INFO @ Tue, 14 Jul 2020 08:09:07: 34000000 INFO @ Tue, 14 Jul 2020 08:09:10: 32000000 INFO @ Tue, 14 Jul 2020 08:09:12: 29000000 INFO @ Tue, 14 Jul 2020 08:09:12: 35000000 INFO @ Tue, 14 Jul 2020 08:09:15: 33000000 INFO @ Tue, 14 Jul 2020 08:09:17: 30000000 INFO @ Tue, 14 Jul 2020 08:09:18: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:09:18: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:09:18: #1 total tags in treatment: 35962379 INFO @ Tue, 14 Jul 2020 08:09:18: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:09:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:09:18: #1 tags after filtering in treatment: 35962379 INFO @ Tue, 14 Jul 2020 08:09:18: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:09:18: #1 finished! INFO @ Tue, 14 Jul 2020 08:09:18: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:09:18: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:09:20: 34000000 INFO @ Tue, 14 Jul 2020 08:09:20: #2 number of paired peaks: 0 WARNING @ Tue, 14 Jul 2020 08:09:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:09:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3008771/SRX3008771.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 14 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008771/SRX3008771.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008771/SRX3008771.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008771/SRX3008771.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:09:22: 31000000 INFO @ Tue, 14 Jul 2020 08:09:25: 35000000 INFO @ Tue, 14 Jul 2020 08:09:27: 32000000 INFO @ Tue, 14 Jul 2020 08:09:30: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:09:30: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:09:30: #1 total tags in treatment: 35962379 INFO @ Tue, 14 Jul 2020 08:09:30: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:09:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:09:31: #1 tags after filtering in treatment: 35962379 INFO @ Tue, 14 Jul 2020 08:09:31: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:09:31: #1 finished! INFO @ Tue, 14 Jul 2020 08:09:31: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:09:31: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:09:32: 33000000 INFO @ Tue, 14 Jul 2020 08:09:33: #2 number of paired peaks: 0 WARNING @ Tue, 14 Jul 2020 08:09:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:09:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3008771/SRX3008771.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008771/SRX3008771.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008771/SRX3008771.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008771/SRX3008771.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:09:37: 34000000 INFO @ Tue, 14 Jul 2020 08:09:42: 35000000 INFO @ Tue, 14 Jul 2020 08:09:47: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:09:47: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:09:47: #1 total tags in treatment: 35962379 INFO @ Tue, 14 Jul 2020 08:09:47: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:09:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:09:48: #1 tags after filtering in treatment: 35962379 INFO @ Tue, 14 Jul 2020 08:09:48: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:09:48: #1 finished! INFO @ Tue, 14 Jul 2020 08:09:48: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:09:48: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:09:50: #2 number of paired peaks: 0 WARNING @ Tue, 14 Jul 2020 08:09:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:09:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3008771/SRX3008771.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008771/SRX3008771.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008771/SRX3008771.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008771/SRX3008771.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。