Job ID = 6626612 SRX = SRX3008766 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:16 60530886 reads; of these: 60530886 (100.00%) were unpaired; of these: 571525 (0.94%) aligned 0 times 48958586 (80.88%) aligned exactly 1 time 11000775 (18.17%) aligned >1 times 99.06% overall alignment rate Time searching: 00:13:16 Overall time: 00:13:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 13922086 / 59959361 = 0.2322 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:17:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3008766/SRX3008766.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3008766/SRX3008766.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3008766/SRX3008766.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3008766/SRX3008766.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:17:27: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:17:27: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:17:33: 1000000 INFO @ Tue, 14 Jul 2020 08:17:39: 2000000 INFO @ Tue, 14 Jul 2020 08:17:44: 3000000 INFO @ Tue, 14 Jul 2020 08:17:50: 4000000 INFO @ Tue, 14 Jul 2020 08:17:55: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:17:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3008766/SRX3008766.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3008766/SRX3008766.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3008766/SRX3008766.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3008766/SRX3008766.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:17:57: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:17:57: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:18:01: 6000000 INFO @ Tue, 14 Jul 2020 08:18:05: 1000000 INFO @ Tue, 14 Jul 2020 08:18:08: 7000000 INFO @ Tue, 14 Jul 2020 08:18:12: 2000000 INFO @ Tue, 14 Jul 2020 08:18:14: 8000000 INFO @ Tue, 14 Jul 2020 08:18:19: 3000000 INFO @ Tue, 14 Jul 2020 08:18:20: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:18:26: 4000000 INFO @ Tue, 14 Jul 2020 08:18:27: 10000000 INFO @ Tue, 14 Jul 2020 08:18:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3008766/SRX3008766.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3008766/SRX3008766.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3008766/SRX3008766.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3008766/SRX3008766.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:18:27: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:18:27: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:18:33: 5000000 INFO @ Tue, 14 Jul 2020 08:18:34: 11000000 INFO @ Tue, 14 Jul 2020 08:18:35: 1000000 INFO @ Tue, 14 Jul 2020 08:18:40: 6000000 INFO @ Tue, 14 Jul 2020 08:18:40: 12000000 INFO @ Tue, 14 Jul 2020 08:18:42: 2000000 INFO @ Tue, 14 Jul 2020 08:18:47: 13000000 INFO @ Tue, 14 Jul 2020 08:18:47: 7000000 INFO @ Tue, 14 Jul 2020 08:18:49: 3000000 INFO @ Tue, 14 Jul 2020 08:18:54: 14000000 INFO @ Tue, 14 Jul 2020 08:18:54: 8000000 INFO @ Tue, 14 Jul 2020 08:18:56: 4000000 INFO @ Tue, 14 Jul 2020 08:19:00: 15000000 INFO @ Tue, 14 Jul 2020 08:19:01: 9000000 INFO @ Tue, 14 Jul 2020 08:19:03: 5000000 INFO @ Tue, 14 Jul 2020 08:19:07: 16000000 INFO @ Tue, 14 Jul 2020 08:19:09: 10000000 INFO @ Tue, 14 Jul 2020 08:19:10: 6000000 INFO @ Tue, 14 Jul 2020 08:19:14: 17000000 INFO @ Tue, 14 Jul 2020 08:19:16: 11000000 INFO @ Tue, 14 Jul 2020 08:19:17: 7000000 INFO @ Tue, 14 Jul 2020 08:19:20: 18000000 INFO @ Tue, 14 Jul 2020 08:19:23: 12000000 INFO @ Tue, 14 Jul 2020 08:19:25: 8000000 INFO @ Tue, 14 Jul 2020 08:19:27: 19000000 INFO @ Tue, 14 Jul 2020 08:19:31: 13000000 INFO @ Tue, 14 Jul 2020 08:19:32: 9000000 INFO @ Tue, 14 Jul 2020 08:19:34: 20000000 INFO @ Tue, 14 Jul 2020 08:19:38: 14000000 INFO @ Tue, 14 Jul 2020 08:19:39: 10000000 INFO @ Tue, 14 Jul 2020 08:19:41: 21000000 INFO @ Tue, 14 Jul 2020 08:19:45: 15000000 INFO @ Tue, 14 Jul 2020 08:19:46: 11000000 INFO @ Tue, 14 Jul 2020 08:19:47: 22000000 INFO @ Tue, 14 Jul 2020 08:19:53: 16000000 INFO @ Tue, 14 Jul 2020 08:19:54: 12000000 INFO @ Tue, 14 Jul 2020 08:19:54: 23000000 INFO @ Tue, 14 Jul 2020 08:20:00: 17000000 INFO @ Tue, 14 Jul 2020 08:20:01: 24000000 INFO @ Tue, 14 Jul 2020 08:20:01: 13000000 INFO @ Tue, 14 Jul 2020 08:20:07: 18000000 INFO @ Tue, 14 Jul 2020 08:20:08: 25000000 INFO @ Tue, 14 Jul 2020 08:20:08: 14000000 INFO @ Tue, 14 Jul 2020 08:20:14: 19000000 INFO @ Tue, 14 Jul 2020 08:20:15: 26000000 INFO @ Tue, 14 Jul 2020 08:20:16: 15000000 INFO @ Tue, 14 Jul 2020 08:20:21: 20000000 INFO @ Tue, 14 Jul 2020 08:20:21: 27000000 INFO @ Tue, 14 Jul 2020 08:20:23: 16000000 INFO @ Tue, 14 Jul 2020 08:20:28: 28000000 INFO @ Tue, 14 Jul 2020 08:20:29: 21000000 INFO @ Tue, 14 Jul 2020 08:20:30: 17000000 INFO @ Tue, 14 Jul 2020 08:20:35: 29000000 INFO @ Tue, 14 Jul 2020 08:20:36: 22000000 INFO @ Tue, 14 Jul 2020 08:20:37: 18000000 INFO @ Tue, 14 Jul 2020 08:20:41: 30000000 INFO @ Tue, 14 Jul 2020 08:20:43: 23000000 INFO @ Tue, 14 Jul 2020 08:20:44: 19000000 INFO @ Tue, 14 Jul 2020 08:20:48: 31000000 INFO @ Tue, 14 Jul 2020 08:20:51: 24000000 INFO @ Tue, 14 Jul 2020 08:20:52: 20000000 INFO @ Tue, 14 Jul 2020 08:20:54: 32000000 INFO @ Tue, 14 Jul 2020 08:20:58: 25000000 INFO @ Tue, 14 Jul 2020 08:20:59: 21000000 INFO @ Tue, 14 Jul 2020 08:21:01: 33000000 INFO @ Tue, 14 Jul 2020 08:21:05: 26000000 INFO @ Tue, 14 Jul 2020 08:21:07: 22000000 INFO @ Tue, 14 Jul 2020 08:21:08: 34000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 08:21:13: 27000000 INFO @ Tue, 14 Jul 2020 08:21:14: 23000000 INFO @ Tue, 14 Jul 2020 08:21:15: 35000000 INFO @ Tue, 14 Jul 2020 08:21:20: 28000000 INFO @ Tue, 14 Jul 2020 08:21:22: 24000000 INFO @ Tue, 14 Jul 2020 08:21:22: 36000000 INFO @ Tue, 14 Jul 2020 08:21:27: 29000000 INFO @ Tue, 14 Jul 2020 08:21:29: 37000000 INFO @ Tue, 14 Jul 2020 08:21:29: 25000000 INFO @ Tue, 14 Jul 2020 08:21:34: 30000000 INFO @ Tue, 14 Jul 2020 08:21:36: 38000000 INFO @ Tue, 14 Jul 2020 08:21:37: 26000000 INFO @ Tue, 14 Jul 2020 08:21:42: 31000000 INFO @ Tue, 14 Jul 2020 08:21:42: 39000000 INFO @ Tue, 14 Jul 2020 08:21:44: 27000000 INFO @ Tue, 14 Jul 2020 08:21:49: 32000000 INFO @ Tue, 14 Jul 2020 08:21:49: 40000000 INFO @ Tue, 14 Jul 2020 08:21:52: 28000000 INFO @ Tue, 14 Jul 2020 08:21:55: 41000000 INFO @ Tue, 14 Jul 2020 08:21:56: 33000000 INFO @ Tue, 14 Jul 2020 08:21:59: 29000000 INFO @ Tue, 14 Jul 2020 08:22:02: 42000000 INFO @ Tue, 14 Jul 2020 08:22:03: 34000000 INFO @ Tue, 14 Jul 2020 08:22:06: 30000000 INFO @ Tue, 14 Jul 2020 08:22:08: 43000000 INFO @ Tue, 14 Jul 2020 08:22:10: 35000000 INFO @ Tue, 14 Jul 2020 08:22:13: 31000000 INFO @ Tue, 14 Jul 2020 08:22:15: 44000000 INFO @ Tue, 14 Jul 2020 08:22:17: 36000000 INFO @ Tue, 14 Jul 2020 08:22:21: 32000000 INFO @ Tue, 14 Jul 2020 08:22:22: 45000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 08:22:24: 37000000 INFO @ Tue, 14 Jul 2020 08:22:28: 33000000 INFO @ Tue, 14 Jul 2020 08:22:28: 46000000 INFO @ Tue, 14 Jul 2020 08:22:29: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:22:29: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:22:29: #1 total tags in treatment: 46037275 INFO @ Tue, 14 Jul 2020 08:22:29: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:22:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:22:29: #1 tags after filtering in treatment: 46037275 INFO @ Tue, 14 Jul 2020 08:22:29: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:22:29: #1 finished! INFO @ Tue, 14 Jul 2020 08:22:29: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:22:29: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:22:31: 38000000 INFO @ Tue, 14 Jul 2020 08:22:32: #2 number of paired peaks: 0 WARNING @ Tue, 14 Jul 2020 08:22:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:22:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3008766/SRX3008766.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008766/SRX3008766.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008766/SRX3008766.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008766/SRX3008766.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:22:35: 34000000 INFO @ Tue, 14 Jul 2020 08:22:38: 39000000 INFO @ Tue, 14 Jul 2020 08:22:42: 35000000 INFO @ Tue, 14 Jul 2020 08:22:45: 40000000 INFO @ Tue, 14 Jul 2020 08:22:48: 36000000 INFO @ Tue, 14 Jul 2020 08:22:52: 41000000 INFO @ Tue, 14 Jul 2020 08:22:55: 37000000 INFO @ Tue, 14 Jul 2020 08:22:59: 42000000 INFO @ Tue, 14 Jul 2020 08:23:02: 38000000 INFO @ Tue, 14 Jul 2020 08:23:06: 43000000 INFO @ Tue, 14 Jul 2020 08:23:09: 39000000 INFO @ Tue, 14 Jul 2020 08:23:13: 44000000 INFO @ Tue, 14 Jul 2020 08:23:16: 40000000 INFO @ Tue, 14 Jul 2020 08:23:19: 45000000 INFO @ Tue, 14 Jul 2020 08:23:23: 41000000 INFO @ Tue, 14 Jul 2020 08:23:26: 46000000 INFO @ Tue, 14 Jul 2020 08:23:27: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:23:27: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:23:27: #1 total tags in treatment: 46037275 INFO @ Tue, 14 Jul 2020 08:23:27: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:23:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:23:28: #1 tags after filtering in treatment: 46037275 INFO @ Tue, 14 Jul 2020 08:23:28: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:23:28: #1 finished! INFO @ Tue, 14 Jul 2020 08:23:28: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:23:28: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:23:30: 42000000 INFO @ Tue, 14 Jul 2020 08:23:30: #2 number of paired peaks: 0 WARNING @ Tue, 14 Jul 2020 08:23:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:23:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3008766/SRX3008766.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008766/SRX3008766.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008766/SRX3008766.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008766/SRX3008766.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:23:36: 43000000 INFO @ Tue, 14 Jul 2020 08:23:43: 44000000 INFO @ Tue, 14 Jul 2020 08:23:50: 45000000 INFO @ Tue, 14 Jul 2020 08:23:56: 46000000 INFO @ Tue, 14 Jul 2020 08:23:57: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:23:57: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:23:57: #1 total tags in treatment: 46037275 INFO @ Tue, 14 Jul 2020 08:23:57: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:23:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:23:57: #1 tags after filtering in treatment: 46037275 INFO @ Tue, 14 Jul 2020 08:23:57: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:23:57: #1 finished! INFO @ Tue, 14 Jul 2020 08:23:57: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:23:57: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:24:00: #2 number of paired peaks: 0 WARNING @ Tue, 14 Jul 2020 08:24:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:24:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3008766/SRX3008766.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008766/SRX3008766.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008766/SRX3008766.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008766/SRX3008766.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling