Job ID = 6367209 SRX = SRX2985929 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:12:01 prefetch.2.10.7: 1) Downloading 'SRR5807079'... 2020-06-15T23:12:01 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:15:14 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:15:14 prefetch.2.10.7: 1) 'SRR5807079' was downloaded successfully 2020-06-15T23:15:14 prefetch.2.10.7: 'SRR5807079' has 0 unresolved dependencies Read 73178255 spots for SRR5807079/SRR5807079.sra Written 73178255 spots for SRR5807079/SRR5807079.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:56 73178255 reads; of these: 73178255 (100.00%) were unpaired; of these: 1291585 (1.76%) aligned 0 times 55284426 (75.55%) aligned exactly 1 time 16602244 (22.69%) aligned >1 times 98.24% overall alignment rate Time searching: 00:16:56 Overall time: 00:16:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 22832667 / 71886670 = 0.3176 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:46:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2985929/SRX2985929.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2985929/SRX2985929.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2985929/SRX2985929.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2985929/SRX2985929.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:46:14: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:46:14: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:46:19: 1000000 INFO @ Tue, 16 Jun 2020 08:46:25: 2000000 INFO @ Tue, 16 Jun 2020 08:46:30: 3000000 INFO @ Tue, 16 Jun 2020 08:46:36: 4000000 INFO @ Tue, 16 Jun 2020 08:46:41: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:46:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2985929/SRX2985929.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2985929/SRX2985929.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2985929/SRX2985929.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2985929/SRX2985929.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:46:44: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:46:44: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:46:47: 6000000 INFO @ Tue, 16 Jun 2020 08:46:51: 1000000 INFO @ Tue, 16 Jun 2020 08:46:53: 7000000 INFO @ Tue, 16 Jun 2020 08:46:58: 2000000 INFO @ Tue, 16 Jun 2020 08:47:00: 8000000 INFO @ Tue, 16 Jun 2020 08:47:05: 3000000 INFO @ Tue, 16 Jun 2020 08:47:06: 9000000 INFO @ Tue, 16 Jun 2020 08:47:11: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:47:12: 10000000 INFO @ Tue, 16 Jun 2020 08:47:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2985929/SRX2985929.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2985929/SRX2985929.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2985929/SRX2985929.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2985929/SRX2985929.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:47:14: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:47:14: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:47:18: 5000000 INFO @ Tue, 16 Jun 2020 08:47:19: 11000000 INFO @ Tue, 16 Jun 2020 08:47:21: 1000000 INFO @ Tue, 16 Jun 2020 08:47:25: 6000000 INFO @ Tue, 16 Jun 2020 08:47:26: 12000000 INFO @ Tue, 16 Jun 2020 08:47:27: 2000000 INFO @ Tue, 16 Jun 2020 08:47:32: 7000000 INFO @ Tue, 16 Jun 2020 08:47:32: 13000000 INFO @ Tue, 16 Jun 2020 08:47:34: 3000000 INFO @ Tue, 16 Jun 2020 08:47:39: 14000000 INFO @ Tue, 16 Jun 2020 08:47:39: 8000000 INFO @ Tue, 16 Jun 2020 08:47:40: 4000000 INFO @ Tue, 16 Jun 2020 08:47:45: 15000000 INFO @ Tue, 16 Jun 2020 08:47:46: 9000000 INFO @ Tue, 16 Jun 2020 08:47:46: 5000000 INFO @ Tue, 16 Jun 2020 08:47:51: 16000000 INFO @ Tue, 16 Jun 2020 08:47:53: 10000000 INFO @ Tue, 16 Jun 2020 08:47:53: 6000000 INFO @ Tue, 16 Jun 2020 08:47:57: 17000000 INFO @ Tue, 16 Jun 2020 08:47:59: 7000000 INFO @ Tue, 16 Jun 2020 08:48:00: 11000000 INFO @ Tue, 16 Jun 2020 08:48:04: 18000000 INFO @ Tue, 16 Jun 2020 08:48:06: 8000000 INFO @ Tue, 16 Jun 2020 08:48:07: 12000000 INFO @ Tue, 16 Jun 2020 08:48:10: 19000000 INFO @ Tue, 16 Jun 2020 08:48:12: 9000000 INFO @ Tue, 16 Jun 2020 08:48:14: 13000000 INFO @ Tue, 16 Jun 2020 08:48:16: 20000000 INFO @ Tue, 16 Jun 2020 08:48:19: 10000000 INFO @ Tue, 16 Jun 2020 08:48:20: 14000000 INFO @ Tue, 16 Jun 2020 08:48:23: 21000000 INFO @ Tue, 16 Jun 2020 08:48:25: 11000000 INFO @ Tue, 16 Jun 2020 08:48:27: 15000000 INFO @ Tue, 16 Jun 2020 08:48:29: 22000000 INFO @ Tue, 16 Jun 2020 08:48:32: 12000000 INFO @ Tue, 16 Jun 2020 08:48:35: 16000000 INFO @ Tue, 16 Jun 2020 08:48:36: 23000000 INFO @ Tue, 16 Jun 2020 08:48:38: 13000000 INFO @ Tue, 16 Jun 2020 08:48:42: 17000000 INFO @ Tue, 16 Jun 2020 08:48:42: 24000000 INFO @ Tue, 16 Jun 2020 08:48:44: 14000000 INFO @ Tue, 16 Jun 2020 08:48:48: 25000000 INFO @ Tue, 16 Jun 2020 08:48:49: 18000000 INFO @ Tue, 16 Jun 2020 08:48:51: 15000000 INFO @ Tue, 16 Jun 2020 08:48:55: 26000000 INFO @ Tue, 16 Jun 2020 08:48:56: 19000000 INFO @ Tue, 16 Jun 2020 08:48:57: 16000000 INFO @ Tue, 16 Jun 2020 08:49:01: 27000000 INFO @ Tue, 16 Jun 2020 08:49:03: 20000000 INFO @ Tue, 16 Jun 2020 08:49:03: 17000000 INFO @ Tue, 16 Jun 2020 08:49:08: 28000000 INFO @ Tue, 16 Jun 2020 08:49:09: 21000000 INFO @ Tue, 16 Jun 2020 08:49:10: 18000000 INFO @ Tue, 16 Jun 2020 08:49:14: 29000000 INFO @ Tue, 16 Jun 2020 08:49:16: 19000000 INFO @ Tue, 16 Jun 2020 08:49:17: 22000000 INFO @ Tue, 16 Jun 2020 08:49:20: 30000000 INFO @ Tue, 16 Jun 2020 08:49:23: 20000000 INFO @ Tue, 16 Jun 2020 08:49:24: 23000000 INFO @ Tue, 16 Jun 2020 08:49:27: 31000000 INFO @ Tue, 16 Jun 2020 08:49:29: 21000000 INFO @ Tue, 16 Jun 2020 08:49:31: 24000000 INFO @ Tue, 16 Jun 2020 08:49:33: 32000000 INFO @ Tue, 16 Jun 2020 08:49:35: 22000000 INFO @ Tue, 16 Jun 2020 08:49:38: 25000000 INFO @ Tue, 16 Jun 2020 08:49:39: 33000000 INFO @ Tue, 16 Jun 2020 08:49:42: 23000000 INFO @ Tue, 16 Jun 2020 08:49:44: 26000000 INFO @ Tue, 16 Jun 2020 08:49:46: 34000000 INFO @ Tue, 16 Jun 2020 08:49:48: 24000000 INFO @ Tue, 16 Jun 2020 08:49:51: 27000000 INFO @ Tue, 16 Jun 2020 08:49:52: 35000000 INFO @ Tue, 16 Jun 2020 08:49:55: 25000000 INFO @ Tue, 16 Jun 2020 08:49:58: 28000000 INFO @ Tue, 16 Jun 2020 08:49:59: 36000000 INFO @ Tue, 16 Jun 2020 08:50:01: 26000000 INFO @ Tue, 16 Jun 2020 08:50:05: 29000000 INFO @ Tue, 16 Jun 2020 08:50:05: 37000000 INFO @ Tue, 16 Jun 2020 08:50:07: 27000000 INFO @ Tue, 16 Jun 2020 08:50:11: 38000000 INFO @ Tue, 16 Jun 2020 08:50:12: 30000000 INFO @ Tue, 16 Jun 2020 08:50:14: 28000000 INFO @ Tue, 16 Jun 2020 08:50:18: 39000000 INFO @ Tue, 16 Jun 2020 08:50:19: 31000000 INFO @ Tue, 16 Jun 2020 08:50:20: 29000000 INFO @ Tue, 16 Jun 2020 08:50:24: 40000000 INFO @ Tue, 16 Jun 2020 08:50:26: 32000000 INFO @ Tue, 16 Jun 2020 08:50:27: 30000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 08:50:31: 41000000 INFO @ Tue, 16 Jun 2020 08:50:33: 33000000 INFO @ Tue, 16 Jun 2020 08:50:33: 31000000 INFO @ Tue, 16 Jun 2020 08:50:37: 42000000 INFO @ Tue, 16 Jun 2020 08:50:39: 32000000 INFO @ Tue, 16 Jun 2020 08:50:40: 34000000 INFO @ Tue, 16 Jun 2020 08:50:43: 43000000 INFO @ Tue, 16 Jun 2020 08:50:46: 33000000 INFO @ Tue, 16 Jun 2020 08:50:47: 35000000 INFO @ Tue, 16 Jun 2020 08:50:50: 44000000 INFO @ Tue, 16 Jun 2020 08:50:52: 34000000 INFO @ Tue, 16 Jun 2020 08:50:54: 36000000 INFO @ Tue, 16 Jun 2020 08:50:56: 45000000 INFO @ Tue, 16 Jun 2020 08:50:59: 35000000 INFO @ Tue, 16 Jun 2020 08:51:01: 37000000 INFO @ Tue, 16 Jun 2020 08:51:03: 46000000 INFO @ Tue, 16 Jun 2020 08:51:05: 36000000 INFO @ Tue, 16 Jun 2020 08:51:08: 38000000 INFO @ Tue, 16 Jun 2020 08:51:09: 47000000 INFO @ Tue, 16 Jun 2020 08:51:11: 37000000 INFO @ Tue, 16 Jun 2020 08:51:15: 39000000 INFO @ Tue, 16 Jun 2020 08:51:15: 48000000 INFO @ Tue, 16 Jun 2020 08:51:18: 38000000 INFO @ Tue, 16 Jun 2020 08:51:22: 49000000 INFO @ Tue, 16 Jun 2020 08:51:22: 40000000 INFO @ Tue, 16 Jun 2020 08:51:22: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 08:51:22: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 08:51:22: #1 total tags in treatment: 49054003 INFO @ Tue, 16 Jun 2020 08:51:22: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:51:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:51:23: #1 tags after filtering in treatment: 49054003 INFO @ Tue, 16 Jun 2020 08:51:23: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:51:23: #1 finished! INFO @ Tue, 16 Jun 2020 08:51:23: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:51:23: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:51:24: 39000000 INFO @ Tue, 16 Jun 2020 08:51:26: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 08:51:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:51:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2985929/SRX2985929.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2985929/SRX2985929.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2985929/SRX2985929.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2985929/SRX2985929.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:51:29: 41000000 INFO @ Tue, 16 Jun 2020 08:51:30: 40000000 INFO @ Tue, 16 Jun 2020 08:51:36: 42000000 INFO @ Tue, 16 Jun 2020 08:51:37: 41000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 08:51:43: 42000000 INFO @ Tue, 16 Jun 2020 08:51:43: 43000000 INFO @ Tue, 16 Jun 2020 08:51:49: 43000000 INFO @ Tue, 16 Jun 2020 08:51:50: 44000000 INFO @ Tue, 16 Jun 2020 08:51:55: 44000000 INFO @ Tue, 16 Jun 2020 08:51:57: 45000000 INFO @ Tue, 16 Jun 2020 08:52:02: 45000000 INFO @ Tue, 16 Jun 2020 08:52:04: 46000000 INFO @ Tue, 16 Jun 2020 08:52:08: 46000000 INFO @ Tue, 16 Jun 2020 08:52:11: 47000000 INFO @ Tue, 16 Jun 2020 08:52:14: 47000000 INFO @ Tue, 16 Jun 2020 08:52:18: 48000000 INFO @ Tue, 16 Jun 2020 08:52:20: 48000000 INFO @ Tue, 16 Jun 2020 08:52:24: 49000000 INFO @ Tue, 16 Jun 2020 08:52:25: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 08:52:25: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 08:52:25: #1 total tags in treatment: 49054003 INFO @ Tue, 16 Jun 2020 08:52:25: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:52:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:52:26: #1 tags after filtering in treatment: 49054003 INFO @ Tue, 16 Jun 2020 08:52:26: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:52:26: #1 finished! INFO @ Tue, 16 Jun 2020 08:52:26: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:52:26: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:52:26: 49000000 INFO @ Tue, 16 Jun 2020 08:52:27: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 08:52:27: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 08:52:27: #1 total tags in treatment: 49054003 INFO @ Tue, 16 Jun 2020 08:52:27: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:52:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:52:28: #1 tags after filtering in treatment: 49054003 INFO @ Tue, 16 Jun 2020 08:52:28: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:52:28: #1 finished! INFO @ Tue, 16 Jun 2020 08:52:28: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:52:28: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:52:29: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 08:52:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:52:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2985929/SRX2985929.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2985929/SRX2985929.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2985929/SRX2985929.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2985929/SRX2985929.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:52:30: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 08:52:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:52:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2985929/SRX2985929.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2985929/SRX2985929.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2985929/SRX2985929.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2985929/SRX2985929.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling