Job ID = 6367196 SRX = SRX2978700 Genome = ce11 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-06-15T23:21:46 prefetch.2.10.7: 1) Downloading 'SRR5799207'... 2020-06-15T23:21:46 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:24:52 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:24:52 prefetch.2.10.7: 1) 'SRR5799207' was downloaded successfully 2020-06-15T23:24:52 prefetch.2.10.7: 'SRR5799207' has 0 unresolved dependencies Read 35676356 spots for SRR5799207/SRR5799207.sra Written 35676356 spots for SRR5799207/SRR5799207.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:31:14 35676356 reads; of these: 35676356 (100.00%) were paired; of these: 3816624 (10.70%) aligned concordantly 0 times 27422997 (76.87%) aligned concordantly exactly 1 time 4436735 (12.44%) aligned concordantly >1 times ---- 3816624 pairs aligned concordantly 0 times; of these: 989712 (25.93%) aligned discordantly 1 time ---- 2826912 pairs aligned 0 times concordantly or discordantly; of these: 5653824 mates make up the pairs; of these: 3667604 (64.87%) aligned 0 times 1435195 (25.38%) aligned exactly 1 time 551025 (9.75%) aligned >1 times 94.86% overall alignment rate Time searching: 00:31:14 Overall time: 00:31:14 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 28 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 5541114 / 32778753 = 0.1690 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:16:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2978700/SRX2978700.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2978700/SRX2978700.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2978700/SRX2978700.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2978700/SRX2978700.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:16:14: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:16:14: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:16:20: 1000000 INFO @ Tue, 16 Jun 2020 09:16:25: 2000000 INFO @ Tue, 16 Jun 2020 09:16:31: 3000000 INFO @ Tue, 16 Jun 2020 09:16:37: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:16:42: 5000000 INFO @ Tue, 16 Jun 2020 09:16:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2978700/SRX2978700.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2978700/SRX2978700.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2978700/SRX2978700.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2978700/SRX2978700.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:16:44: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:16:44: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:16:48: 6000000 INFO @ Tue, 16 Jun 2020 09:16:50: 1000000 INFO @ Tue, 16 Jun 2020 09:16:53: 7000000 INFO @ Tue, 16 Jun 2020 09:16:55: 2000000 INFO @ Tue, 16 Jun 2020 09:16:59: 8000000 INFO @ Tue, 16 Jun 2020 09:17:00: 3000000 INFO @ Tue, 16 Jun 2020 09:17:05: 9000000 INFO @ Tue, 16 Jun 2020 09:17:06: 4000000 INFO @ Tue, 16 Jun 2020 09:17:10: 10000000 INFO @ Tue, 16 Jun 2020 09:17:11: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:17:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2978700/SRX2978700.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2978700/SRX2978700.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2978700/SRX2978700.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2978700/SRX2978700.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:17:14: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:17:14: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:17:16: 11000000 INFO @ Tue, 16 Jun 2020 09:17:16: 6000000 INFO @ Tue, 16 Jun 2020 09:17:20: 1000000 INFO @ Tue, 16 Jun 2020 09:17:21: 7000000 INFO @ Tue, 16 Jun 2020 09:17:21: 12000000 INFO @ Tue, 16 Jun 2020 09:17:25: 2000000 INFO @ Tue, 16 Jun 2020 09:17:27: 8000000 INFO @ Tue, 16 Jun 2020 09:17:27: 13000000 INFO @ Tue, 16 Jun 2020 09:17:30: 3000000 INFO @ Tue, 16 Jun 2020 09:17:32: 9000000 INFO @ Tue, 16 Jun 2020 09:17:32: 14000000 INFO @ Tue, 16 Jun 2020 09:17:36: 4000000 INFO @ Tue, 16 Jun 2020 09:17:37: 10000000 INFO @ Tue, 16 Jun 2020 09:17:38: 15000000 INFO @ Tue, 16 Jun 2020 09:17:41: 5000000 INFO @ Tue, 16 Jun 2020 09:17:42: 11000000 INFO @ Tue, 16 Jun 2020 09:17:44: 16000000 INFO @ Tue, 16 Jun 2020 09:17:47: 6000000 INFO @ Tue, 16 Jun 2020 09:17:47: 12000000 INFO @ Tue, 16 Jun 2020 09:17:49: 17000000 INFO @ Tue, 16 Jun 2020 09:17:52: 7000000 INFO @ Tue, 16 Jun 2020 09:17:52: 13000000 INFO @ Tue, 16 Jun 2020 09:17:55: 18000000 INFO @ Tue, 16 Jun 2020 09:17:57: 8000000 INFO @ Tue, 16 Jun 2020 09:17:58: 14000000 INFO @ Tue, 16 Jun 2020 09:18:00: 19000000 INFO @ Tue, 16 Jun 2020 09:18:02: 9000000 INFO @ Tue, 16 Jun 2020 09:18:03: 15000000 INFO @ Tue, 16 Jun 2020 09:18:06: 20000000 INFO @ Tue, 16 Jun 2020 09:18:08: 10000000 INFO @ Tue, 16 Jun 2020 09:18:08: 16000000 INFO @ Tue, 16 Jun 2020 09:18:11: 21000000 INFO @ Tue, 16 Jun 2020 09:18:13: 11000000 INFO @ Tue, 16 Jun 2020 09:18:13: 17000000 INFO @ Tue, 16 Jun 2020 09:18:17: 22000000 INFO @ Tue, 16 Jun 2020 09:18:18: 12000000 INFO @ Tue, 16 Jun 2020 09:18:18: 18000000 INFO @ Tue, 16 Jun 2020 09:18:23: 23000000 INFO @ Tue, 16 Jun 2020 09:18:23: 13000000 INFO @ Tue, 16 Jun 2020 09:18:23: 19000000 INFO @ Tue, 16 Jun 2020 09:18:28: 24000000 INFO @ Tue, 16 Jun 2020 09:18:29: 20000000 INFO @ Tue, 16 Jun 2020 09:18:29: 14000000 INFO @ Tue, 16 Jun 2020 09:18:34: 21000000 INFO @ Tue, 16 Jun 2020 09:18:34: 15000000 INFO @ Tue, 16 Jun 2020 09:18:34: 25000000 INFO @ Tue, 16 Jun 2020 09:18:39: 22000000 INFO @ Tue, 16 Jun 2020 09:18:39: 16000000 INFO @ Tue, 16 Jun 2020 09:18:40: 26000000 INFO @ Tue, 16 Jun 2020 09:18:44: 23000000 INFO @ Tue, 16 Jun 2020 09:18:45: 17000000 INFO @ Tue, 16 Jun 2020 09:18:45: 27000000 INFO @ Tue, 16 Jun 2020 09:18:50: 24000000 INFO @ Tue, 16 Jun 2020 09:18:50: 18000000 INFO @ Tue, 16 Jun 2020 09:18:51: 28000000 INFO @ Tue, 16 Jun 2020 09:18:55: 25000000 INFO @ Tue, 16 Jun 2020 09:18:56: 19000000 INFO @ Tue, 16 Jun 2020 09:18:57: 29000000 INFO @ Tue, 16 Jun 2020 09:19:00: 26000000 INFO @ Tue, 16 Jun 2020 09:19:01: 20000000 INFO @ Tue, 16 Jun 2020 09:19:03: 30000000 INFO @ Tue, 16 Jun 2020 09:19:05: 27000000 INFO @ Tue, 16 Jun 2020 09:19:07: 21000000 INFO @ Tue, 16 Jun 2020 09:19:09: 31000000 INFO @ Tue, 16 Jun 2020 09:19:11: 28000000 INFO @ Tue, 16 Jun 2020 09:19:12: 22000000 INFO @ Tue, 16 Jun 2020 09:19:15: 32000000 INFO @ Tue, 16 Jun 2020 09:19:16: 29000000 INFO @ Tue, 16 Jun 2020 09:19:18: 23000000 INFO @ Tue, 16 Jun 2020 09:19:21: 33000000 INFO @ Tue, 16 Jun 2020 09:19:21: 30000000 INFO @ Tue, 16 Jun 2020 09:19:23: 24000000 INFO @ Tue, 16 Jun 2020 09:19:27: 31000000 INFO @ Tue, 16 Jun 2020 09:19:27: 34000000 INFO @ Tue, 16 Jun 2020 09:19:29: 25000000 INFO @ Tue, 16 Jun 2020 09:19:32: 32000000 INFO @ Tue, 16 Jun 2020 09:19:33: 35000000 INFO @ Tue, 16 Jun 2020 09:19:35: 26000000 INFO @ Tue, 16 Jun 2020 09:19:37: 33000000 INFO @ Tue, 16 Jun 2020 09:19:39: 36000000 INFO @ Tue, 16 Jun 2020 09:19:40: 27000000 INFO @ Tue, 16 Jun 2020 09:19:43: 34000000 INFO @ Tue, 16 Jun 2020 09:19:45: 37000000 INFO @ Tue, 16 Jun 2020 09:19:45: 28000000 INFO @ Tue, 16 Jun 2020 09:19:48: 35000000 INFO @ Tue, 16 Jun 2020 09:19:51: 29000000 INFO @ Tue, 16 Jun 2020 09:19:51: 38000000 INFO @ Tue, 16 Jun 2020 09:19:53: 36000000 INFO @ Tue, 16 Jun 2020 09:19:56: 30000000 INFO @ Tue, 16 Jun 2020 09:19:57: 39000000 INFO @ Tue, 16 Jun 2020 09:19:59: 37000000 INFO @ Tue, 16 Jun 2020 09:20:02: 31000000 INFO @ Tue, 16 Jun 2020 09:20:03: 40000000 INFO @ Tue, 16 Jun 2020 09:20:04: 38000000 INFO @ Tue, 16 Jun 2020 09:20:08: 32000000 INFO @ Tue, 16 Jun 2020 09:20:09: 41000000 INFO @ Tue, 16 Jun 2020 09:20:09: 39000000 INFO @ Tue, 16 Jun 2020 09:20:13: 33000000 INFO @ Tue, 16 Jun 2020 09:20:15: 42000000 INFO @ Tue, 16 Jun 2020 09:20:15: 40000000 INFO @ Tue, 16 Jun 2020 09:20:18: 34000000 INFO @ Tue, 16 Jun 2020 09:20:20: 41000000 INFO @ Tue, 16 Jun 2020 09:20:20: 43000000 INFO @ Tue, 16 Jun 2020 09:20:24: 35000000 INFO @ Tue, 16 Jun 2020 09:20:25: 42000000 INFO @ Tue, 16 Jun 2020 09:20:26: 44000000 INFO @ Tue, 16 Jun 2020 09:20:29: 36000000 INFO @ Tue, 16 Jun 2020 09:20:30: 43000000 INFO @ Tue, 16 Jun 2020 09:20:32: 45000000 INFO @ Tue, 16 Jun 2020 09:20:35: 37000000 INFO @ Tue, 16 Jun 2020 09:20:36: 44000000 INFO @ Tue, 16 Jun 2020 09:20:38: 46000000 INFO @ Tue, 16 Jun 2020 09:20:40: 38000000 INFO @ Tue, 16 Jun 2020 09:20:41: 45000000 INFO @ Tue, 16 Jun 2020 09:20:43: 47000000 INFO @ Tue, 16 Jun 2020 09:20:46: 39000000 INFO @ Tue, 16 Jun 2020 09:20:46: 46000000 INFO @ Tue, 16 Jun 2020 09:20:49: 48000000 INFO @ Tue, 16 Jun 2020 09:20:51: 40000000 INFO @ Tue, 16 Jun 2020 09:20:51: 47000000 INFO @ Tue, 16 Jun 2020 09:20:54: 49000000 INFO @ Tue, 16 Jun 2020 09:20:56: 41000000 INFO @ Tue, 16 Jun 2020 09:20:56: 48000000 INFO @ Tue, 16 Jun 2020 09:21:00: 50000000 INFO @ Tue, 16 Jun 2020 09:21:01: 49000000 INFO @ Tue, 16 Jun 2020 09:21:02: 42000000 INFO @ Tue, 16 Jun 2020 09:21:06: 51000000 INFO @ Tue, 16 Jun 2020 09:21:07: 50000000 INFO @ Tue, 16 Jun 2020 09:21:07: 43000000 INFO @ Tue, 16 Jun 2020 09:21:11: 52000000 INFO @ Tue, 16 Jun 2020 09:21:12: 51000000 INFO @ Tue, 16 Jun 2020 09:21:12: 44000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:21:17: 53000000 INFO @ Tue, 16 Jun 2020 09:21:17: 52000000 INFO @ Tue, 16 Jun 2020 09:21:18: 45000000 INFO @ Tue, 16 Jun 2020 09:21:22: 53000000 INFO @ Tue, 16 Jun 2020 09:21:22: 54000000 INFO @ Tue, 16 Jun 2020 09:21:23: 46000000 INFO @ Tue, 16 Jun 2020 09:21:27: 54000000 INFO @ Tue, 16 Jun 2020 09:21:28: 55000000 INFO @ Tue, 16 Jun 2020 09:21:28: 47000000 INFO @ Tue, 16 Jun 2020 09:21:32: 55000000 INFO @ Tue, 16 Jun 2020 09:21:33: 56000000 INFO @ Tue, 16 Jun 2020 09:21:34: 48000000 INFO @ Tue, 16 Jun 2020 09:21:36: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:21:37: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:21:37: #1 total tags in treatment: 26407001 INFO @ Tue, 16 Jun 2020 09:21:37: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:21:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:21:37: #1 tags after filtering in treatment: 23434561 INFO @ Tue, 16 Jun 2020 09:21:37: #1 Redundant rate of treatment: 0.11 INFO @ Tue, 16 Jun 2020 09:21:37: #1 finished! INFO @ Tue, 16 Jun 2020 09:21:37: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:21:37: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:21:37: 56000000 INFO @ Tue, 16 Jun 2020 09:21:38: #2 number of paired peaks: 210 WARNING @ Tue, 16 Jun 2020 09:21:38: Fewer paired peaks (210) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 210 pairs to build model! INFO @ Tue, 16 Jun 2020 09:21:38: start model_add_line... INFO @ Tue, 16 Jun 2020 09:21:39: start X-correlation... INFO @ Tue, 16 Jun 2020 09:21:39: end of X-cor INFO @ Tue, 16 Jun 2020 09:21:39: #2 finished! INFO @ Tue, 16 Jun 2020 09:21:39: #2 predicted fragment length is 90 bps INFO @ Tue, 16 Jun 2020 09:21:39: #2 alternative fragment length(s) may be 2,54,78,83,90 bps INFO @ Tue, 16 Jun 2020 09:21:39: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX2978700/SRX2978700.05_model.r WARNING @ Tue, 16 Jun 2020 09:21:39: #2 Since the d (90) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 09:21:39: #2 You may need to consider one of the other alternative d(s): 2,54,78,83,90 WARNING @ Tue, 16 Jun 2020 09:21:39: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 09:21:39: #3 Call peaks... INFO @ Tue, 16 Jun 2020 09:21:39: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 09:21:39: 49000000 INFO @ Tue, 16 Jun 2020 09:21:40: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:21:40: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:21:40: #1 total tags in treatment: 26407001 INFO @ Tue, 16 Jun 2020 09:21:40: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:21:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:21:41: #1 tags after filtering in treatment: 23434561 INFO @ Tue, 16 Jun 2020 09:21:41: #1 Redundant rate of treatment: 0.11 INFO @ Tue, 16 Jun 2020 09:21:41: #1 finished! INFO @ Tue, 16 Jun 2020 09:21:41: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:21:41: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:21:42: #2 number of paired peaks: 210 WARNING @ Tue, 16 Jun 2020 09:21:42: Fewer paired peaks (210) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 210 pairs to build model! INFO @ Tue, 16 Jun 2020 09:21:42: start model_add_line... INFO @ Tue, 16 Jun 2020 09:21:43: start X-correlation... INFO @ Tue, 16 Jun 2020 09:21:43: end of X-cor INFO @ Tue, 16 Jun 2020 09:21:43: #2 finished! INFO @ Tue, 16 Jun 2020 09:21:43: #2 predicted fragment length is 90 bps INFO @ Tue, 16 Jun 2020 09:21:43: #2 alternative fragment length(s) may be 2,54,78,83,90 bps INFO @ Tue, 16 Jun 2020 09:21:43: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX2978700/SRX2978700.10_model.r WARNING @ Tue, 16 Jun 2020 09:21:43: #2 Since the d (90) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 09:21:43: #2 You may need to consider one of the other alternative d(s): 2,54,78,83,90 WARNING @ Tue, 16 Jun 2020 09:21:43: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 09:21:43: #3 Call peaks... INFO @ Tue, 16 Jun 2020 09:21:43: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 09:21:44: 50000000 INFO @ Tue, 16 Jun 2020 09:21:49: 51000000 INFO @ Tue, 16 Jun 2020 09:21:54: 52000000 INFO @ Tue, 16 Jun 2020 09:21:58: 53000000 INFO @ Tue, 16 Jun 2020 09:22:03: 54000000 INFO @ Tue, 16 Jun 2020 09:22:08: 55000000 INFO @ Tue, 16 Jun 2020 09:22:13: 56000000 INFO @ Tue, 16 Jun 2020 09:22:16: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:22:16: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:22:16: #1 total tags in treatment: 26407001 INFO @ Tue, 16 Jun 2020 09:22:16: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:22:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:22:16: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 09:22:16: #1 tags after filtering in treatment: 23434561 INFO @ Tue, 16 Jun 2020 09:22:16: #1 Redundant rate of treatment: 0.11 INFO @ Tue, 16 Jun 2020 09:22:16: #1 finished! INFO @ Tue, 16 Jun 2020 09:22:16: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:22:16: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:22:18: #2 number of paired peaks: 210 WARNING @ Tue, 16 Jun 2020 09:22:18: Fewer paired peaks (210) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 210 pairs to build model! INFO @ Tue, 16 Jun 2020 09:22:18: start model_add_line... INFO @ Tue, 16 Jun 2020 09:22:18: start X-correlation... INFO @ Tue, 16 Jun 2020 09:22:18: end of X-cor INFO @ Tue, 16 Jun 2020 09:22:18: #2 finished! INFO @ Tue, 16 Jun 2020 09:22:18: #2 predicted fragment length is 90 bps INFO @ Tue, 16 Jun 2020 09:22:18: #2 alternative fragment length(s) may be 2,54,78,83,90 bps INFO @ Tue, 16 Jun 2020 09:22:18: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX2978700/SRX2978700.20_model.r WARNING @ Tue, 16 Jun 2020 09:22:18: #2 Since the d (90) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 09:22:18: #2 You may need to consider one of the other alternative d(s): 2,54,78,83,90 WARNING @ Tue, 16 Jun 2020 09:22:18: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 09:22:18: #3 Call peaks... INFO @ Tue, 16 Jun 2020 09:22:18: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 09:22:22: #3 Call peaks for each chromosome... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 09:22:35: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX2978700/SRX2978700.05_peaks.xls INFO @ Tue, 16 Jun 2020 09:22:35: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX2978700/SRX2978700.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 09:22:35: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX2978700/SRX2978700.05_summits.bed INFO @ Tue, 16 Jun 2020 09:22:35: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (555 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:22:40: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX2978700/SRX2978700.10_peaks.xls INFO @ Tue, 16 Jun 2020 09:22:40: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX2978700/SRX2978700.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 09:22:40: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX2978700/SRX2978700.10_summits.bed INFO @ Tue, 16 Jun 2020 09:22:40: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (381 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:22:57: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 09:23:17: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX2978700/SRX2978700.20_peaks.xls INFO @ Tue, 16 Jun 2020 09:23:17: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX2978700/SRX2978700.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 09:23:17: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX2978700/SRX2978700.20_summits.bed INFO @ Tue, 16 Jun 2020 09:23:17: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (219 records, 4 fields): 2 millis CompletedMACS2peakCalling