Job ID = 6367169 SRX = SRX2965743 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:40:14 prefetch.2.10.7: 1) Downloading 'SRR5766328'... 2020-06-15T23:40:14 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:51:11 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:51:11 prefetch.2.10.7: 1) 'SRR5766328' was downloaded successfully Read 45310834 spots for SRR5766328/SRR5766328.sra Written 45310834 spots for SRR5766328/SRR5766328.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:18:12 45310834 reads; of these: 45310834 (100.00%) were unpaired; of these: 9131095 (20.15%) aligned 0 times 31241501 (68.95%) aligned exactly 1 time 4938238 (10.90%) aligned >1 times 79.85% overall alignment rate Time searching: 00:18:12 Overall time: 00:18:12 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 6696646 / 36179739 = 0.1851 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:23:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2965743/SRX2965743.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2965743/SRX2965743.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2965743/SRX2965743.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2965743/SRX2965743.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:23:21: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:23:21: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:23:29: 1000000 INFO @ Tue, 16 Jun 2020 09:23:37: 2000000 INFO @ Tue, 16 Jun 2020 09:23:46: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:23:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2965743/SRX2965743.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2965743/SRX2965743.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2965743/SRX2965743.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2965743/SRX2965743.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:23:51: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:23:51: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:23:54: 4000000 INFO @ Tue, 16 Jun 2020 09:23:59: 1000000 INFO @ Tue, 16 Jun 2020 09:24:03: 5000000 INFO @ Tue, 16 Jun 2020 09:24:07: 2000000 INFO @ Tue, 16 Jun 2020 09:24:12: 6000000 INFO @ Tue, 16 Jun 2020 09:24:15: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:24:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2965743/SRX2965743.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2965743/SRX2965743.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2965743/SRX2965743.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2965743/SRX2965743.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:24:21: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:24:21: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:24:21: 7000000 INFO @ Tue, 16 Jun 2020 09:24:24: 4000000 INFO @ Tue, 16 Jun 2020 09:24:30: 1000000 INFO @ Tue, 16 Jun 2020 09:24:30: 8000000 INFO @ Tue, 16 Jun 2020 09:24:32: 5000000 INFO @ Tue, 16 Jun 2020 09:24:39: 2000000 INFO @ Tue, 16 Jun 2020 09:24:39: 9000000 INFO @ Tue, 16 Jun 2020 09:24:40: 6000000 INFO @ Tue, 16 Jun 2020 09:24:48: 3000000 INFO @ Tue, 16 Jun 2020 09:24:48: 10000000 INFO @ Tue, 16 Jun 2020 09:24:49: 7000000 INFO @ Tue, 16 Jun 2020 09:24:57: 8000000 INFO @ Tue, 16 Jun 2020 09:24:57: 4000000 INFO @ Tue, 16 Jun 2020 09:24:57: 11000000 INFO @ Tue, 16 Jun 2020 09:25:05: 9000000 INFO @ Tue, 16 Jun 2020 09:25:06: 5000000 INFO @ Tue, 16 Jun 2020 09:25:06: 12000000 INFO @ Tue, 16 Jun 2020 09:25:13: 10000000 INFO @ Tue, 16 Jun 2020 09:25:15: 13000000 INFO @ Tue, 16 Jun 2020 09:25:16: 6000000 INFO @ Tue, 16 Jun 2020 09:25:21: 11000000 INFO @ Tue, 16 Jun 2020 09:25:24: 14000000 INFO @ Tue, 16 Jun 2020 09:25:25: 7000000 INFO @ Tue, 16 Jun 2020 09:25:30: 12000000 INFO @ Tue, 16 Jun 2020 09:25:33: 8000000 INFO @ Tue, 16 Jun 2020 09:25:33: 15000000 INFO @ Tue, 16 Jun 2020 09:25:38: 13000000 INFO @ Tue, 16 Jun 2020 09:25:42: 9000000 INFO @ Tue, 16 Jun 2020 09:25:42: 16000000 INFO @ Tue, 16 Jun 2020 09:25:46: 14000000 INFO @ Tue, 16 Jun 2020 09:25:51: 10000000 INFO @ Tue, 16 Jun 2020 09:25:51: 17000000 INFO @ Tue, 16 Jun 2020 09:25:54: 15000000 INFO @ Tue, 16 Jun 2020 09:26:00: 18000000 INFO @ Tue, 16 Jun 2020 09:26:00: 11000000 INFO @ Tue, 16 Jun 2020 09:26:03: 16000000 INFO @ Tue, 16 Jun 2020 09:26:09: 19000000 INFO @ Tue, 16 Jun 2020 09:26:10: 12000000 INFO @ Tue, 16 Jun 2020 09:26:11: 17000000 INFO @ Tue, 16 Jun 2020 09:26:19: 20000000 INFO @ Tue, 16 Jun 2020 09:26:19: 13000000 INFO @ Tue, 16 Jun 2020 09:26:19: 18000000 INFO @ Tue, 16 Jun 2020 09:26:27: 19000000 INFO @ Tue, 16 Jun 2020 09:26:28: 21000000 INFO @ Tue, 16 Jun 2020 09:26:28: 14000000 INFO @ Tue, 16 Jun 2020 09:26:36: 20000000 INFO @ Tue, 16 Jun 2020 09:26:37: 15000000 INFO @ Tue, 16 Jun 2020 09:26:37: 22000000 INFO @ Tue, 16 Jun 2020 09:26:44: 21000000 INFO @ Tue, 16 Jun 2020 09:26:46: 23000000 INFO @ Tue, 16 Jun 2020 09:26:46: 16000000 INFO @ Tue, 16 Jun 2020 09:26:52: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:26:55: 24000000 INFO @ Tue, 16 Jun 2020 09:26:55: 17000000 INFO @ Tue, 16 Jun 2020 09:27:00: 23000000 INFO @ Tue, 16 Jun 2020 09:27:04: 25000000 INFO @ Tue, 16 Jun 2020 09:27:04: 18000000 INFO @ Tue, 16 Jun 2020 09:27:09: 24000000 INFO @ Tue, 16 Jun 2020 09:27:13: 26000000 INFO @ Tue, 16 Jun 2020 09:27:13: 19000000 INFO @ Tue, 16 Jun 2020 09:27:17: 25000000 INFO @ Tue, 16 Jun 2020 09:27:22: 27000000 INFO @ Tue, 16 Jun 2020 09:27:22: 20000000 INFO @ Tue, 16 Jun 2020 09:27:25: 26000000 INFO @ Tue, 16 Jun 2020 09:27:30: 28000000 INFO @ Tue, 16 Jun 2020 09:27:32: 21000000 INFO @ Tue, 16 Jun 2020 09:27:33: 27000000 INFO @ Tue, 16 Jun 2020 09:27:39: 29000000 INFO @ Tue, 16 Jun 2020 09:27:41: 22000000 INFO @ Tue, 16 Jun 2020 09:27:41: 28000000 INFO @ Tue, 16 Jun 2020 09:27:44: #1 tag size is determined as 101 bps INFO @ Tue, 16 Jun 2020 09:27:44: #1 tag size = 101 INFO @ Tue, 16 Jun 2020 09:27:44: #1 total tags in treatment: 29483093 INFO @ Tue, 16 Jun 2020 09:27:44: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:27:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:27:44: #1 tags after filtering in treatment: 29483093 INFO @ Tue, 16 Jun 2020 09:27:44: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:27:44: #1 finished! INFO @ Tue, 16 Jun 2020 09:27:44: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:27:44: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:27:46: #2 number of paired peaks: 49 WARNING @ Tue, 16 Jun 2020 09:27:46: Too few paired peaks (49) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:27:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2965743/SRX2965743.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965743/SRX2965743.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965743/SRX2965743.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965743/SRX2965743.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 09:27:49: 29000000 INFO @ Tue, 16 Jun 2020 09:27:50: 23000000 INFO @ Tue, 16 Jun 2020 09:27:53: #1 tag size is determined as 101 bps INFO @ Tue, 16 Jun 2020 09:27:53: #1 tag size = 101 INFO @ Tue, 16 Jun 2020 09:27:53: #1 total tags in treatment: 29483093 INFO @ Tue, 16 Jun 2020 09:27:53: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:27:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:27:54: #1 tags after filtering in treatment: 29483093 INFO @ Tue, 16 Jun 2020 09:27:54: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:27:54: #1 finished! INFO @ Tue, 16 Jun 2020 09:27:54: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:27:54: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:27:56: #2 number of paired peaks: 49 WARNING @ Tue, 16 Jun 2020 09:27:56: Too few paired peaks (49) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:27:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2965743/SRX2965743.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965743/SRX2965743.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965743/SRX2965743.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965743/SRX2965743.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:27:58: 24000000 INFO @ Tue, 16 Jun 2020 09:28:06: 25000000 INFO @ Tue, 16 Jun 2020 09:28:14: 26000000 INFO @ Tue, 16 Jun 2020 09:28:22: 27000000 INFO @ Tue, 16 Jun 2020 09:28:30: 28000000 INFO @ Tue, 16 Jun 2020 09:28:39: 29000000 INFO @ Tue, 16 Jun 2020 09:28:43: #1 tag size is determined as 101 bps INFO @ Tue, 16 Jun 2020 09:28:43: #1 tag size = 101 INFO @ Tue, 16 Jun 2020 09:28:43: #1 total tags in treatment: 29483093 INFO @ Tue, 16 Jun 2020 09:28:43: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:28:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:28:43: #1 tags after filtering in treatment: 29483093 INFO @ Tue, 16 Jun 2020 09:28:43: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:28:43: #1 finished! INFO @ Tue, 16 Jun 2020 09:28:43: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:28:43: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:28:45: #2 number of paired peaks: 49 WARNING @ Tue, 16 Jun 2020 09:28:45: Too few paired peaks (49) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:28:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2965743/SRX2965743.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965743/SRX2965743.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965743/SRX2965743.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965743/SRX2965743.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling