Job ID = 6367165 SRX = SRX2965739 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:13:46 prefetch.2.10.7: 1) Downloading 'SRR5766324'... 2020-06-15T23:13:46 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:18:12 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:18:12 prefetch.2.10.7: 1) 'SRR5766324' was downloaded successfully 2020-06-15T23:18:12 prefetch.2.10.7: 'SRR5766324' has 0 unresolved dependencies Read 54119838 spots for SRR5766324/SRR5766324.sra Written 54119838 spots for SRR5766324/SRR5766324.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:17:48 54119838 reads; of these: 54119838 (100.00%) were unpaired; of these: 1596435 (2.95%) aligned 0 times 44662870 (82.53%) aligned exactly 1 time 7860533 (14.52%) aligned >1 times 97.05% overall alignment rate Time searching: 00:17:48 Overall time: 00:17:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 13339298 / 52523403 = 0.2540 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:51:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2965739/SRX2965739.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2965739/SRX2965739.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2965739/SRX2965739.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2965739/SRX2965739.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:51:14: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:51:14: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:51:22: 1000000 INFO @ Tue, 16 Jun 2020 08:51:31: 2000000 INFO @ Tue, 16 Jun 2020 08:51:40: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:51:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2965739/SRX2965739.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2965739/SRX2965739.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2965739/SRX2965739.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2965739/SRX2965739.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:51:44: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:51:44: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:51:49: 4000000 INFO @ Tue, 16 Jun 2020 08:51:54: 1000000 INFO @ Tue, 16 Jun 2020 08:51:58: 5000000 INFO @ Tue, 16 Jun 2020 08:52:04: 2000000 INFO @ Tue, 16 Jun 2020 08:52:08: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:52:13: 3000000 INFO @ Tue, 16 Jun 2020 08:52:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2965739/SRX2965739.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2965739/SRX2965739.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2965739/SRX2965739.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2965739/SRX2965739.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:52:14: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:52:14: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:52:17: 7000000 INFO @ Tue, 16 Jun 2020 08:52:23: 1000000 INFO @ Tue, 16 Jun 2020 08:52:23: 4000000 INFO @ Tue, 16 Jun 2020 08:52:28: 8000000 INFO @ Tue, 16 Jun 2020 08:52:32: 2000000 INFO @ Tue, 16 Jun 2020 08:52:33: 5000000 INFO @ Tue, 16 Jun 2020 08:52:37: 9000000 INFO @ Tue, 16 Jun 2020 08:52:41: 3000000 INFO @ Tue, 16 Jun 2020 08:52:43: 6000000 INFO @ Tue, 16 Jun 2020 08:52:47: 10000000 INFO @ Tue, 16 Jun 2020 08:52:50: 4000000 INFO @ Tue, 16 Jun 2020 08:52:53: 7000000 INFO @ Tue, 16 Jun 2020 08:52:57: 11000000 INFO @ Tue, 16 Jun 2020 08:52:59: 5000000 INFO @ Tue, 16 Jun 2020 08:53:03: 8000000 INFO @ Tue, 16 Jun 2020 08:53:07: 12000000 INFO @ Tue, 16 Jun 2020 08:53:08: 6000000 INFO @ Tue, 16 Jun 2020 08:53:12: 9000000 INFO @ Tue, 16 Jun 2020 08:53:16: 13000000 INFO @ Tue, 16 Jun 2020 08:53:17: 7000000 INFO @ Tue, 16 Jun 2020 08:53:22: 10000000 INFO @ Tue, 16 Jun 2020 08:53:26: 8000000 INFO @ Tue, 16 Jun 2020 08:53:26: 14000000 INFO @ Tue, 16 Jun 2020 08:53:32: 11000000 INFO @ Tue, 16 Jun 2020 08:53:35: 9000000 INFO @ Tue, 16 Jun 2020 08:53:35: 15000000 INFO @ Tue, 16 Jun 2020 08:53:41: 12000000 INFO @ Tue, 16 Jun 2020 08:53:43: 10000000 INFO @ Tue, 16 Jun 2020 08:53:45: 16000000 INFO @ Tue, 16 Jun 2020 08:53:51: 13000000 INFO @ Tue, 16 Jun 2020 08:53:52: 11000000 INFO @ Tue, 16 Jun 2020 08:53:54: 17000000 INFO @ Tue, 16 Jun 2020 08:54:01: 14000000 INFO @ Tue, 16 Jun 2020 08:54:01: 12000000 INFO @ Tue, 16 Jun 2020 08:54:04: 18000000 INFO @ Tue, 16 Jun 2020 08:54:10: 13000000 INFO @ Tue, 16 Jun 2020 08:54:10: 15000000 INFO @ Tue, 16 Jun 2020 08:54:14: 19000000 INFO @ Tue, 16 Jun 2020 08:54:19: 14000000 INFO @ Tue, 16 Jun 2020 08:54:20: 16000000 INFO @ Tue, 16 Jun 2020 08:54:23: 20000000 INFO @ Tue, 16 Jun 2020 08:54:28: 15000000 INFO @ Tue, 16 Jun 2020 08:54:29: 17000000 INFO @ Tue, 16 Jun 2020 08:54:33: 21000000 INFO @ Tue, 16 Jun 2020 08:54:37: 16000000 INFO @ Tue, 16 Jun 2020 08:54:39: 18000000 INFO @ Tue, 16 Jun 2020 08:54:43: 22000000 INFO @ Tue, 16 Jun 2020 08:54:46: 17000000 INFO @ Tue, 16 Jun 2020 08:54:49: 19000000 INFO @ Tue, 16 Jun 2020 08:54:52: 23000000 INFO @ Tue, 16 Jun 2020 08:54:55: 18000000 INFO @ Tue, 16 Jun 2020 08:54:59: 20000000 INFO @ Tue, 16 Jun 2020 08:55:02: 24000000 INFO @ Tue, 16 Jun 2020 08:55:04: 19000000 INFO @ Tue, 16 Jun 2020 08:55:09: 21000000 INFO @ Tue, 16 Jun 2020 08:55:12: 25000000 INFO @ Tue, 16 Jun 2020 08:55:13: 20000000 INFO @ Tue, 16 Jun 2020 08:55:18: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 08:55:22: 26000000 INFO @ Tue, 16 Jun 2020 08:55:22: 21000000 INFO @ Tue, 16 Jun 2020 08:55:28: 23000000 INFO @ Tue, 16 Jun 2020 08:55:31: 22000000 INFO @ Tue, 16 Jun 2020 08:55:32: 27000000 INFO @ Tue, 16 Jun 2020 08:55:38: 24000000 INFO @ Tue, 16 Jun 2020 08:55:40: 23000000 INFO @ Tue, 16 Jun 2020 08:55:42: 28000000 INFO @ Tue, 16 Jun 2020 08:55:48: 25000000 INFO @ Tue, 16 Jun 2020 08:55:49: 24000000 INFO @ Tue, 16 Jun 2020 08:55:51: 29000000 INFO @ Tue, 16 Jun 2020 08:55:58: 26000000 INFO @ Tue, 16 Jun 2020 08:55:58: 25000000 INFO @ Tue, 16 Jun 2020 08:56:01: 30000000 INFO @ Tue, 16 Jun 2020 08:56:07: 27000000 INFO @ Tue, 16 Jun 2020 08:56:07: 26000000 INFO @ Tue, 16 Jun 2020 08:56:10: 31000000 INFO @ Tue, 16 Jun 2020 08:56:16: 27000000 INFO @ Tue, 16 Jun 2020 08:56:17: 28000000 INFO @ Tue, 16 Jun 2020 08:56:19: 32000000 INFO @ Tue, 16 Jun 2020 08:56:26: 28000000 INFO @ Tue, 16 Jun 2020 08:56:27: 29000000 INFO @ Tue, 16 Jun 2020 08:56:29: 33000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 08:56:35: 29000000 INFO @ Tue, 16 Jun 2020 08:56:36: 30000000 INFO @ Tue, 16 Jun 2020 08:56:39: 34000000 INFO @ Tue, 16 Jun 2020 08:56:44: 30000000 INFO @ Tue, 16 Jun 2020 08:56:46: 31000000 INFO @ Tue, 16 Jun 2020 08:56:49: 35000000 INFO @ Tue, 16 Jun 2020 08:56:53: 31000000 INFO @ Tue, 16 Jun 2020 08:56:55: 32000000 INFO @ Tue, 16 Jun 2020 08:56:58: 36000000 INFO @ Tue, 16 Jun 2020 08:57:02: 32000000 INFO @ Tue, 16 Jun 2020 08:57:05: 33000000 INFO @ Tue, 16 Jun 2020 08:57:08: 37000000 INFO @ Tue, 16 Jun 2020 08:57:11: 33000000 INFO @ Tue, 16 Jun 2020 08:57:15: 34000000 INFO @ Tue, 16 Jun 2020 08:57:18: 38000000 INFO @ Tue, 16 Jun 2020 08:57:20: 34000000 INFO @ Tue, 16 Jun 2020 08:57:25: 35000000 INFO @ Tue, 16 Jun 2020 08:57:28: 39000000 INFO @ Tue, 16 Jun 2020 08:57:29: 35000000 INFO @ Tue, 16 Jun 2020 08:57:30: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 08:57:30: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 08:57:30: #1 total tags in treatment: 39184105 INFO @ Tue, 16 Jun 2020 08:57:30: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:57:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:57:31: #1 tags after filtering in treatment: 39184105 INFO @ Tue, 16 Jun 2020 08:57:31: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:57:31: #1 finished! INFO @ Tue, 16 Jun 2020 08:57:31: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:57:31: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:57:33: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 08:57:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:57:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2965739/SRX2965739.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965739/SRX2965739.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965739/SRX2965739.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965739/SRX2965739.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:57:35: 36000000 INFO @ Tue, 16 Jun 2020 08:57:37: 36000000 INFO @ Tue, 16 Jun 2020 08:57:45: 37000000 INFO @ Tue, 16 Jun 2020 08:57:46: 37000000 INFO @ Tue, 16 Jun 2020 08:57:55: 38000000 INFO @ Tue, 16 Jun 2020 08:57:56: 38000000 INFO @ Tue, 16 Jun 2020 08:58:05: 39000000 INFO @ Tue, 16 Jun 2020 08:58:06: 39000000 INFO @ Tue, 16 Jun 2020 08:58:07: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 08:58:07: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 08:58:07: #1 total tags in treatment: 39184105 INFO @ Tue, 16 Jun 2020 08:58:07: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:58:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:58:07: #1 tags after filtering in treatment: 39184105 INFO @ Tue, 16 Jun 2020 08:58:07: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:58:07: #1 finished! INFO @ Tue, 16 Jun 2020 08:58:07: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:58:07: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:58:08: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 08:58:08: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 08:58:08: #1 total tags in treatment: 39184105 INFO @ Tue, 16 Jun 2020 08:58:08: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:58:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:58:08: #1 tags after filtering in treatment: 39184105 INFO @ Tue, 16 Jun 2020 08:58:08: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:58:08: #1 finished! INFO @ Tue, 16 Jun 2020 08:58:08: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:58:08: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:58:10: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 08:58:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:58:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2965739/SRX2965739.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965739/SRX2965739.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965739/SRX2965739.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965739/SRX2965739.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:58:11: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 08:58:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:58:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2965739/SRX2965739.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965739/SRX2965739.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965739/SRX2965739.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965739/SRX2965739.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling