Job ID = 6367162 SRX = SRX2965736 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:22:46 prefetch.2.10.7: 1) Downloading 'SRR5766321'... 2020-06-15T23:22:46 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:28:32 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:28:32 prefetch.2.10.7: 1) 'SRR5766321' was downloaded successfully 2020-06-15T23:28:32 prefetch.2.10.7: 'SRR5766321' has 0 unresolved dependencies Read 71862577 spots for SRR5766321/SRR5766321.sra Written 71862577 spots for SRR5766321/SRR5766321.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:24:43 71862577 reads; of these: 71862577 (100.00%) were unpaired; of these: 4849049 (6.75%) aligned 0 times 56182321 (78.18%) aligned exactly 1 time 10831207 (15.07%) aligned >1 times 93.25% overall alignment rate Time searching: 00:24:43 Overall time: 00:24:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 22904865 / 67013528 = 0.3418 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:11:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2965736/SRX2965736.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2965736/SRX2965736.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2965736/SRX2965736.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2965736/SRX2965736.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:11:35: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:11:35: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:11:42: 1000000 INFO @ Tue, 16 Jun 2020 09:11:49: 2000000 INFO @ Tue, 16 Jun 2020 09:11:56: 3000000 INFO @ Tue, 16 Jun 2020 09:12:02: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:12:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2965736/SRX2965736.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2965736/SRX2965736.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2965736/SRX2965736.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2965736/SRX2965736.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:12:05: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:12:05: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:12:10: 5000000 INFO @ Tue, 16 Jun 2020 09:12:14: 1000000 INFO @ Tue, 16 Jun 2020 09:12:17: 6000000 INFO @ Tue, 16 Jun 2020 09:12:22: 2000000 INFO @ Tue, 16 Jun 2020 09:12:25: 7000000 INFO @ Tue, 16 Jun 2020 09:12:30: 3000000 INFO @ Tue, 16 Jun 2020 09:12:33: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:12:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2965736/SRX2965736.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2965736/SRX2965736.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2965736/SRX2965736.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2965736/SRX2965736.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:12:35: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:12:35: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:12:37: 4000000 INFO @ Tue, 16 Jun 2020 09:12:40: 9000000 INFO @ Tue, 16 Jun 2020 09:12:43: 1000000 INFO @ Tue, 16 Jun 2020 09:12:45: 5000000 INFO @ Tue, 16 Jun 2020 09:12:48: 10000000 INFO @ Tue, 16 Jun 2020 09:12:51: 2000000 INFO @ Tue, 16 Jun 2020 09:12:53: 6000000 INFO @ Tue, 16 Jun 2020 09:12:56: 11000000 INFO @ Tue, 16 Jun 2020 09:12:59: 3000000 INFO @ Tue, 16 Jun 2020 09:13:01: 7000000 INFO @ Tue, 16 Jun 2020 09:13:04: 12000000 INFO @ Tue, 16 Jun 2020 09:13:07: 4000000 INFO @ Tue, 16 Jun 2020 09:13:09: 8000000 INFO @ Tue, 16 Jun 2020 09:13:12: 13000000 INFO @ Tue, 16 Jun 2020 09:13:15: 5000000 INFO @ Tue, 16 Jun 2020 09:13:17: 9000000 INFO @ Tue, 16 Jun 2020 09:13:20: 14000000 INFO @ Tue, 16 Jun 2020 09:13:22: 6000000 INFO @ Tue, 16 Jun 2020 09:13:25: 10000000 INFO @ Tue, 16 Jun 2020 09:13:28: 15000000 INFO @ Tue, 16 Jun 2020 09:13:30: 7000000 INFO @ Tue, 16 Jun 2020 09:13:33: 11000000 INFO @ Tue, 16 Jun 2020 09:13:36: 16000000 INFO @ Tue, 16 Jun 2020 09:13:38: 8000000 INFO @ Tue, 16 Jun 2020 09:13:41: 12000000 INFO @ Tue, 16 Jun 2020 09:13:44: 17000000 INFO @ Tue, 16 Jun 2020 09:13:46: 9000000 INFO @ Tue, 16 Jun 2020 09:13:48: 13000000 INFO @ Tue, 16 Jun 2020 09:13:53: 18000000 INFO @ Tue, 16 Jun 2020 09:13:54: 10000000 INFO @ Tue, 16 Jun 2020 09:13:57: 14000000 INFO @ Tue, 16 Jun 2020 09:14:02: 19000000 INFO @ Tue, 16 Jun 2020 09:14:02: 11000000 INFO @ Tue, 16 Jun 2020 09:14:05: 15000000 INFO @ Tue, 16 Jun 2020 09:14:10: 12000000 INFO @ Tue, 16 Jun 2020 09:14:10: 20000000 INFO @ Tue, 16 Jun 2020 09:14:13: 16000000 INFO @ Tue, 16 Jun 2020 09:14:18: 13000000 INFO @ Tue, 16 Jun 2020 09:14:20: 21000000 INFO @ Tue, 16 Jun 2020 09:14:20: 17000000 INFO @ Tue, 16 Jun 2020 09:14:26: 14000000 INFO @ Tue, 16 Jun 2020 09:14:28: 22000000 INFO @ Tue, 16 Jun 2020 09:14:29: 18000000 INFO @ Tue, 16 Jun 2020 09:14:34: 15000000 INFO @ Tue, 16 Jun 2020 09:14:37: 23000000 INFO @ Tue, 16 Jun 2020 09:14:37: 19000000 INFO @ Tue, 16 Jun 2020 09:14:42: 16000000 INFO @ Tue, 16 Jun 2020 09:14:45: 24000000 INFO @ Tue, 16 Jun 2020 09:14:46: 20000000 INFO @ Tue, 16 Jun 2020 09:14:50: 17000000 INFO @ Tue, 16 Jun 2020 09:14:54: 25000000 INFO @ Tue, 16 Jun 2020 09:14:54: 21000000 INFO @ Tue, 16 Jun 2020 09:14:58: 18000000 INFO @ Tue, 16 Jun 2020 09:15:02: 26000000 INFO @ Tue, 16 Jun 2020 09:15:02: 22000000 INFO @ Tue, 16 Jun 2020 09:15:07: 19000000 INFO @ Tue, 16 Jun 2020 09:15:11: 23000000 INFO @ Tue, 16 Jun 2020 09:15:11: 27000000 INFO @ Tue, 16 Jun 2020 09:15:15: 20000000 INFO @ Tue, 16 Jun 2020 09:15:19: 24000000 INFO @ Tue, 16 Jun 2020 09:15:20: 28000000 INFO @ Tue, 16 Jun 2020 09:15:24: 21000000 INFO @ Tue, 16 Jun 2020 09:15:27: 25000000 INFO @ Tue, 16 Jun 2020 09:15:28: 29000000 INFO @ Tue, 16 Jun 2020 09:15:32: 22000000 INFO @ Tue, 16 Jun 2020 09:15:36: 26000000 INFO @ Tue, 16 Jun 2020 09:15:37: 30000000 INFO @ Tue, 16 Jun 2020 09:15:41: 23000000 INFO @ Tue, 16 Jun 2020 09:15:45: 27000000 INFO @ Tue, 16 Jun 2020 09:15:45: 31000000 INFO @ Tue, 16 Jun 2020 09:15:49: 24000000 INFO @ Tue, 16 Jun 2020 09:15:53: 28000000 INFO @ Tue, 16 Jun 2020 09:15:54: 32000000 INFO @ Tue, 16 Jun 2020 09:15:58: 25000000 INFO @ Tue, 16 Jun 2020 09:16:02: 29000000 INFO @ Tue, 16 Jun 2020 09:16:03: 33000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:16:07: 26000000 INFO @ Tue, 16 Jun 2020 09:16:11: 30000000 INFO @ Tue, 16 Jun 2020 09:16:12: 34000000 INFO @ Tue, 16 Jun 2020 09:16:16: 27000000 INFO @ Tue, 16 Jun 2020 09:16:19: 31000000 INFO @ Tue, 16 Jun 2020 09:16:21: 35000000 INFO @ Tue, 16 Jun 2020 09:16:25: 28000000 INFO @ Tue, 16 Jun 2020 09:16:28: 32000000 INFO @ Tue, 16 Jun 2020 09:16:30: 36000000 INFO @ Tue, 16 Jun 2020 09:16:34: 29000000 INFO @ Tue, 16 Jun 2020 09:16:37: 33000000 INFO @ Tue, 16 Jun 2020 09:16:39: 37000000 INFO @ Tue, 16 Jun 2020 09:16:42: 30000000 INFO @ Tue, 16 Jun 2020 09:16:46: 34000000 INFO @ Tue, 16 Jun 2020 09:16:48: 38000000 INFO @ Tue, 16 Jun 2020 09:16:51: 31000000 INFO @ Tue, 16 Jun 2020 09:16:54: 35000000 INFO @ Tue, 16 Jun 2020 09:16:57: 39000000 INFO @ Tue, 16 Jun 2020 09:17:00: 32000000 INFO @ Tue, 16 Jun 2020 09:17:03: 36000000 INFO @ Tue, 16 Jun 2020 09:17:06: 40000000 INFO @ Tue, 16 Jun 2020 09:17:08: 33000000 INFO @ Tue, 16 Jun 2020 09:17:12: 37000000 INFO @ Tue, 16 Jun 2020 09:17:14: 41000000 INFO @ Tue, 16 Jun 2020 09:17:17: 34000000 INFO @ Tue, 16 Jun 2020 09:17:21: 38000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 09:17:23: 42000000 INFO @ Tue, 16 Jun 2020 09:17:25: 35000000 INFO @ Tue, 16 Jun 2020 09:17:30: 39000000 INFO @ Tue, 16 Jun 2020 09:17:32: 43000000 INFO @ Tue, 16 Jun 2020 09:17:34: 36000000 INFO @ Tue, 16 Jun 2020 09:17:39: 40000000 INFO @ Tue, 16 Jun 2020 09:17:41: 44000000 INFO @ Tue, 16 Jun 2020 09:17:42: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 09:17:42: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 09:17:42: #1 total tags in treatment: 44108663 INFO @ Tue, 16 Jun 2020 09:17:42: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:17:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:17:43: #1 tags after filtering in treatment: 44108663 INFO @ Tue, 16 Jun 2020 09:17:43: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:17:43: #1 finished! INFO @ Tue, 16 Jun 2020 09:17:43: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:17:43: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:17:43: 37000000 INFO @ Tue, 16 Jun 2020 09:17:45: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:17:45: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:17:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2965736/SRX2965736.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965736/SRX2965736.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965736/SRX2965736.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965736/SRX2965736.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:17:48: 41000000 INFO @ Tue, 16 Jun 2020 09:17:51: 38000000 INFO @ Tue, 16 Jun 2020 09:17:56: 42000000 INFO @ Tue, 16 Jun 2020 09:18:00: 39000000 INFO @ Tue, 16 Jun 2020 09:18:05: 43000000 INFO @ Tue, 16 Jun 2020 09:18:09: 40000000 INFO @ Tue, 16 Jun 2020 09:18:13: 44000000 INFO @ Tue, 16 Jun 2020 09:18:14: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 09:18:14: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 09:18:14: #1 total tags in treatment: 44108663 INFO @ Tue, 16 Jun 2020 09:18:14: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:18:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:18:15: #1 tags after filtering in treatment: 44108663 INFO @ Tue, 16 Jun 2020 09:18:15: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:18:15: #1 finished! INFO @ Tue, 16 Jun 2020 09:18:15: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:18:15: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:18:17: 41000000 INFO @ Tue, 16 Jun 2020 09:18:18: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:18:18: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:18:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2965736/SRX2965736.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965736/SRX2965736.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965736/SRX2965736.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965736/SRX2965736.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:18:25: 42000000 INFO @ Tue, 16 Jun 2020 09:18:33: 43000000 INFO @ Tue, 16 Jun 2020 09:18:40: 44000000 INFO @ Tue, 16 Jun 2020 09:18:41: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 09:18:41: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 09:18:41: #1 total tags in treatment: 44108663 INFO @ Tue, 16 Jun 2020 09:18:41: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:18:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:18:42: #1 tags after filtering in treatment: 44108663 INFO @ Tue, 16 Jun 2020 09:18:42: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:18:42: #1 finished! INFO @ Tue, 16 Jun 2020 09:18:42: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:18:42: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:18:45: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:18:45: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:18:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2965736/SRX2965736.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965736/SRX2965736.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965736/SRX2965736.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965736/SRX2965736.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling