Job ID = 6367030 SRX = SRX2737089 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:14:18 prefetch.2.10.7: 1) Downloading 'SRR5448127'... 2020-06-15T23:14:18 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:16:30 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:16:30 prefetch.2.10.7: 1) 'SRR5448127' was downloaded successfully 2020-06-15T23:16:30 prefetch.2.10.7: 'SRR5448127' has 0 unresolved dependencies Read 36121620 spots for SRR5448127/SRR5448127.sra Written 36121620 spots for SRR5448127/SRR5448127.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:02 36121620 reads; of these: 36121620 (100.00%) were unpaired; of these: 702373 (1.94%) aligned 0 times 29696484 (82.21%) aligned exactly 1 time 5722763 (15.84%) aligned >1 times 98.06% overall alignment rate Time searching: 00:08:02 Overall time: 00:08:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 5262015 / 35419247 = 0.1486 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:32:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2737089/SRX2737089.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2737089/SRX2737089.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2737089/SRX2737089.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2737089/SRX2737089.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:32:40: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:32:40: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:32:45: 1000000 INFO @ Tue, 16 Jun 2020 08:32:50: 2000000 INFO @ Tue, 16 Jun 2020 08:32:54: 3000000 INFO @ Tue, 16 Jun 2020 08:32:59: 4000000 INFO @ Tue, 16 Jun 2020 08:33:04: 5000000 INFO @ Tue, 16 Jun 2020 08:33:08: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:33:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2737089/SRX2737089.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2737089/SRX2737089.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2737089/SRX2737089.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2737089/SRX2737089.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:33:10: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:33:10: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:33:13: 7000000 INFO @ Tue, 16 Jun 2020 08:33:15: 1000000 INFO @ Tue, 16 Jun 2020 08:33:18: 8000000 INFO @ Tue, 16 Jun 2020 08:33:20: 2000000 INFO @ Tue, 16 Jun 2020 08:33:22: 9000000 INFO @ Tue, 16 Jun 2020 08:33:25: 3000000 INFO @ Tue, 16 Jun 2020 08:33:27: 10000000 INFO @ Tue, 16 Jun 2020 08:33:29: 4000000 INFO @ Tue, 16 Jun 2020 08:33:31: 11000000 INFO @ Tue, 16 Jun 2020 08:33:34: 5000000 INFO @ Tue, 16 Jun 2020 08:33:36: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:33:39: 6000000 INFO @ Tue, 16 Jun 2020 08:33:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2737089/SRX2737089.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2737089/SRX2737089.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2737089/SRX2737089.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2737089/SRX2737089.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:33:40: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:33:40: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:33:41: 13000000 INFO @ Tue, 16 Jun 2020 08:33:44: 7000000 INFO @ Tue, 16 Jun 2020 08:33:45: 14000000 INFO @ Tue, 16 Jun 2020 08:33:46: 1000000 INFO @ Tue, 16 Jun 2020 08:33:49: 8000000 INFO @ Tue, 16 Jun 2020 08:33:50: 15000000 INFO @ Tue, 16 Jun 2020 08:33:51: 2000000 INFO @ Tue, 16 Jun 2020 08:33:54: 9000000 INFO @ Tue, 16 Jun 2020 08:33:55: 16000000 INFO @ Tue, 16 Jun 2020 08:33:56: 3000000 INFO @ Tue, 16 Jun 2020 08:33:59: 10000000 INFO @ Tue, 16 Jun 2020 08:33:59: 17000000 INFO @ Tue, 16 Jun 2020 08:34:02: 4000000 INFO @ Tue, 16 Jun 2020 08:34:04: 11000000 INFO @ Tue, 16 Jun 2020 08:34:04: 18000000 INFO @ Tue, 16 Jun 2020 08:34:07: 5000000 INFO @ Tue, 16 Jun 2020 08:34:08: 12000000 INFO @ Tue, 16 Jun 2020 08:34:09: 19000000 INFO @ Tue, 16 Jun 2020 08:34:12: 6000000 INFO @ Tue, 16 Jun 2020 08:34:13: 20000000 INFO @ Tue, 16 Jun 2020 08:34:13: 13000000 INFO @ Tue, 16 Jun 2020 08:34:17: 7000000 INFO @ Tue, 16 Jun 2020 08:34:18: 21000000 INFO @ Tue, 16 Jun 2020 08:34:18: 14000000 INFO @ Tue, 16 Jun 2020 08:34:22: 22000000 INFO @ Tue, 16 Jun 2020 08:34:22: 8000000 INFO @ Tue, 16 Jun 2020 08:34:23: 15000000 INFO @ Tue, 16 Jun 2020 08:34:27: 23000000 INFO @ Tue, 16 Jun 2020 08:34:27: 16000000 INFO @ Tue, 16 Jun 2020 08:34:28: 9000000 INFO @ Tue, 16 Jun 2020 08:34:31: 24000000 INFO @ Tue, 16 Jun 2020 08:34:32: 17000000 INFO @ Tue, 16 Jun 2020 08:34:33: 10000000 INFO @ Tue, 16 Jun 2020 08:34:36: 25000000 INFO @ Tue, 16 Jun 2020 08:34:36: 18000000 INFO @ Tue, 16 Jun 2020 08:34:38: 11000000 INFO @ Tue, 16 Jun 2020 08:34:40: 26000000 INFO @ Tue, 16 Jun 2020 08:34:41: 19000000 INFO @ Tue, 16 Jun 2020 08:34:43: 12000000 INFO @ Tue, 16 Jun 2020 08:34:45: 27000000 INFO @ Tue, 16 Jun 2020 08:34:45: 20000000 INFO @ Tue, 16 Jun 2020 08:34:49: 13000000 INFO @ Tue, 16 Jun 2020 08:34:49: 28000000 INFO @ Tue, 16 Jun 2020 08:34:50: 21000000 INFO @ Tue, 16 Jun 2020 08:34:54: 29000000 INFO @ Tue, 16 Jun 2020 08:34:54: 14000000 INFO @ Tue, 16 Jun 2020 08:34:55: 22000000 INFO @ Tue, 16 Jun 2020 08:34:58: 30000000 INFO @ Tue, 16 Jun 2020 08:34:59: 23000000 INFO @ Tue, 16 Jun 2020 08:34:59: 15000000 INFO @ Tue, 16 Jun 2020 08:34:59: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 08:34:59: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 08:34:59: #1 total tags in treatment: 30157232 INFO @ Tue, 16 Jun 2020 08:34:59: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:34:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:35:00: #1 tags after filtering in treatment: 30157232 INFO @ Tue, 16 Jun 2020 08:35:00: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:35:00: #1 finished! INFO @ Tue, 16 Jun 2020 08:35:00: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:35:00: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:35:01: #2 number of paired peaks: 80 WARNING @ Tue, 16 Jun 2020 08:35:01: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:35:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2737089/SRX2737089.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2737089/SRX2737089.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2737089/SRX2737089.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2737089/SRX2737089.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:35:03: 24000000 INFO @ Tue, 16 Jun 2020 08:35:04: 16000000 INFO @ Tue, 16 Jun 2020 08:35:08: 25000000 INFO @ Tue, 16 Jun 2020 08:35:09: 17000000 INFO @ Tue, 16 Jun 2020 08:35:13: 26000000 INFO @ Tue, 16 Jun 2020 08:35:14: 18000000 INFO @ Tue, 16 Jun 2020 08:35:17: 27000000 INFO @ Tue, 16 Jun 2020 08:35:20: 19000000 INFO @ Tue, 16 Jun 2020 08:35:22: 28000000 INFO @ Tue, 16 Jun 2020 08:35:25: 20000000 INFO @ Tue, 16 Jun 2020 08:35:26: 29000000 INFO @ Tue, 16 Jun 2020 08:35:30: 21000000 INFO @ Tue, 16 Jun 2020 08:35:31: 30000000 INFO @ Tue, 16 Jun 2020 08:35:32: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 08:35:32: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 08:35:32: #1 total tags in treatment: 30157232 INFO @ Tue, 16 Jun 2020 08:35:32: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:35:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:35:32: #1 tags after filtering in treatment: 30157232 INFO @ Tue, 16 Jun 2020 08:35:32: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:35:32: #1 finished! INFO @ Tue, 16 Jun 2020 08:35:32: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:35:32: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:35:34: #2 number of paired peaks: 80 WARNING @ Tue, 16 Jun 2020 08:35:34: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:35:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2737089/SRX2737089.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2737089/SRX2737089.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2737089/SRX2737089.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2737089/SRX2737089.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:35:35: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 08:35:40: 23000000 INFO @ Tue, 16 Jun 2020 08:35:45: 24000000 INFO @ Tue, 16 Jun 2020 08:35:50: 25000000 INFO @ Tue, 16 Jun 2020 08:35:55: 26000000 INFO @ Tue, 16 Jun 2020 08:36:00: 27000000 INFO @ Tue, 16 Jun 2020 08:36:05: 28000000 INFO @ Tue, 16 Jun 2020 08:36:10: 29000000 INFO @ Tue, 16 Jun 2020 08:36:15: 30000000 INFO @ Tue, 16 Jun 2020 08:36:16: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 08:36:16: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 08:36:16: #1 total tags in treatment: 30157232 INFO @ Tue, 16 Jun 2020 08:36:16: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:36:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:36:17: #1 tags after filtering in treatment: 30157232 INFO @ Tue, 16 Jun 2020 08:36:17: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:36:17: #1 finished! INFO @ Tue, 16 Jun 2020 08:36:17: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:36:17: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:36:18: #2 number of paired peaks: 80 WARNING @ Tue, 16 Jun 2020 08:36:18: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:36:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2737089/SRX2737089.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2737089/SRX2737089.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2737089/SRX2737089.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2737089/SRX2737089.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。