Job ID = 6366861 SRX = SRX235169 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:05:38 prefetch.2.10.7: 1) Downloading 'SRR708617'... 2020-06-15T23:05:38 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:05:59 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:05:59 prefetch.2.10.7: 'SRR708617' is valid 2020-06-15T23:05:59 prefetch.2.10.7: 1) 'SRR708617' was downloaded successfully Read 4382589 spots for SRR708617/SRR708617.sra Written 4382589 spots for SRR708617/SRR708617.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:32 4382589 reads; of these: 4382589 (100.00%) were unpaired; of these: 1691226 (38.59%) aligned 0 times 2215494 (50.55%) aligned exactly 1 time 475869 (10.86%) aligned >1 times 61.41% overall alignment rate Time searching: 00:00:32 Overall time: 00:00:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 655469 / 2691363 = 0.2435 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:07:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX235169/SRX235169.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX235169/SRX235169.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX235169/SRX235169.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX235169/SRX235169.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:07:43: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:07:43: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:07:48: 1000000 INFO @ Tue, 16 Jun 2020 08:07:53: 2000000 INFO @ Tue, 16 Jun 2020 08:07:53: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 08:07:53: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 08:07:53: #1 total tags in treatment: 2035894 INFO @ Tue, 16 Jun 2020 08:07:53: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:07:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:07:53: #1 tags after filtering in treatment: 2035894 INFO @ Tue, 16 Jun 2020 08:07:53: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:07:53: #1 finished! INFO @ Tue, 16 Jun 2020 08:07:53: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:07:53: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:07:53: #2 number of paired peaks: 477 WARNING @ Tue, 16 Jun 2020 08:07:53: Fewer paired peaks (477) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 477 pairs to build model! INFO @ Tue, 16 Jun 2020 08:07:53: start model_add_line... INFO @ Tue, 16 Jun 2020 08:07:53: start X-correlation... INFO @ Tue, 16 Jun 2020 08:07:53: end of X-cor INFO @ Tue, 16 Jun 2020 08:07:53: #2 finished! INFO @ Tue, 16 Jun 2020 08:07:53: #2 predicted fragment length is 34 bps INFO @ Tue, 16 Jun 2020 08:07:53: #2 alternative fragment length(s) may be 4,34,88,476,553,569,571 bps INFO @ Tue, 16 Jun 2020 08:07:53: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX235169/SRX235169.05_model.r WARNING @ Tue, 16 Jun 2020 08:07:53: #2 Since the d (34) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 08:07:53: #2 You may need to consider one of the other alternative d(s): 4,34,88,476,553,569,571 WARNING @ Tue, 16 Jun 2020 08:07:53: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 08:07:53: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:07:53: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 08:07:58: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 08:08:00: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX235169/SRX235169.05_peaks.xls INFO @ Tue, 16 Jun 2020 08:08:00: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX235169/SRX235169.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:08:00: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX235169/SRX235169.05_summits.bed INFO @ Tue, 16 Jun 2020 08:08:00: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (312 records, 4 fields): 1 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:08:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX235169/SRX235169.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX235169/SRX235169.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX235169/SRX235169.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX235169/SRX235169.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:08:13: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:08:13: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:08:18: 1000000 INFO @ Tue, 16 Jun 2020 08:08:23: 2000000 INFO @ Tue, 16 Jun 2020 08:08:23: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 08:08:23: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 08:08:23: #1 total tags in treatment: 2035894 INFO @ Tue, 16 Jun 2020 08:08:23: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:08:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:08:23: #1 tags after filtering in treatment: 2035894 INFO @ Tue, 16 Jun 2020 08:08:23: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:08:23: #1 finished! INFO @ Tue, 16 Jun 2020 08:08:23: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:08:23: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:08:23: #2 number of paired peaks: 477 WARNING @ Tue, 16 Jun 2020 08:08:23: Fewer paired peaks (477) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 477 pairs to build model! INFO @ Tue, 16 Jun 2020 08:08:23: start model_add_line... INFO @ Tue, 16 Jun 2020 08:08:23: start X-correlation... INFO @ Tue, 16 Jun 2020 08:08:23: end of X-cor INFO @ Tue, 16 Jun 2020 08:08:23: #2 finished! INFO @ Tue, 16 Jun 2020 08:08:23: #2 predicted fragment length is 34 bps INFO @ Tue, 16 Jun 2020 08:08:23: #2 alternative fragment length(s) may be 4,34,88,476,553,569,571 bps INFO @ Tue, 16 Jun 2020 08:08:23: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX235169/SRX235169.10_model.r WARNING @ Tue, 16 Jun 2020 08:08:23: #2 Since the d (34) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 08:08:23: #2 You may need to consider one of the other alternative d(s): 4,34,88,476,553,569,571 WARNING @ Tue, 16 Jun 2020 08:08:23: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 08:08:23: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:08:23: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 08:08:28: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 08:08:30: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX235169/SRX235169.10_peaks.xls INFO @ Tue, 16 Jun 2020 08:08:30: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX235169/SRX235169.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:08:30: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX235169/SRX235169.10_summits.bed INFO @ Tue, 16 Jun 2020 08:08:30: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (146 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:08:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX235169/SRX235169.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX235169/SRX235169.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX235169/SRX235169.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX235169/SRX235169.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:08:43: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:08:43: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:08:48: 1000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 08:08:54: 2000000 INFO @ Tue, 16 Jun 2020 08:08:54: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 08:08:54: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 08:08:54: #1 total tags in treatment: 2035894 INFO @ Tue, 16 Jun 2020 08:08:54: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:08:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:08:54: #1 tags after filtering in treatment: 2035894 INFO @ Tue, 16 Jun 2020 08:08:54: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:08:54: #1 finished! INFO @ Tue, 16 Jun 2020 08:08:54: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:08:54: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:08:54: #2 number of paired peaks: 477 WARNING @ Tue, 16 Jun 2020 08:08:54: Fewer paired peaks (477) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 477 pairs to build model! INFO @ Tue, 16 Jun 2020 08:08:54: start model_add_line... INFO @ Tue, 16 Jun 2020 08:08:54: start X-correlation... INFO @ Tue, 16 Jun 2020 08:08:54: end of X-cor INFO @ Tue, 16 Jun 2020 08:08:54: #2 finished! INFO @ Tue, 16 Jun 2020 08:08:54: #2 predicted fragment length is 34 bps INFO @ Tue, 16 Jun 2020 08:08:54: #2 alternative fragment length(s) may be 4,34,88,476,553,569,571 bps INFO @ Tue, 16 Jun 2020 08:08:54: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX235169/SRX235169.20_model.r WARNING @ Tue, 16 Jun 2020 08:08:54: #2 Since the d (34) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 08:08:54: #2 You may need to consider one of the other alternative d(s): 4,34,88,476,553,569,571 WARNING @ Tue, 16 Jun 2020 08:08:54: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 08:08:54: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:08:54: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 08:08:59: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 08:09:01: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX235169/SRX235169.20_peaks.xls INFO @ Tue, 16 Jun 2020 08:09:01: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX235169/SRX235169.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:09:01: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX235169/SRX235169.20_summits.bed INFO @ Tue, 16 Jun 2020 08:09:01: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (24 records, 4 fields): 1 millis CompletedMACS2peakCalling