Job ID = 6366801 SRX = SRX233696 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:11:16 prefetch.2.10.7: 1) Downloading 'SRR702399'... 2020-06-15T23:11:16 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:11:57 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:11:57 prefetch.2.10.7: 'SRR702399' is valid 2020-06-15T23:11:57 prefetch.2.10.7: 1) 'SRR702399' was downloaded successfully Read 6721950 spots for SRR702399/SRR702399.sra Written 6721950 spots for SRR702399/SRR702399.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:47 6721950 reads; of these: 6721950 (100.00%) were unpaired; of these: 2924016 (43.50%) aligned 0 times 3285712 (48.88%) aligned exactly 1 time 512222 (7.62%) aligned >1 times 56.50% overall alignment rate Time searching: 00:00:47 Overall time: 00:00:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 452291 / 3797934 = 0.1191 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:14:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX233696/SRX233696.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX233696/SRX233696.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX233696/SRX233696.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX233696/SRX233696.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:14:24: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:14:24: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:14:30: 1000000 INFO @ Tue, 16 Jun 2020 08:14:35: 2000000 INFO @ Tue, 16 Jun 2020 08:14:40: 3000000 INFO @ Tue, 16 Jun 2020 08:14:42: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 08:14:42: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 08:14:42: #1 total tags in treatment: 3345643 INFO @ Tue, 16 Jun 2020 08:14:42: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:14:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:14:42: #1 tags after filtering in treatment: 3345643 INFO @ Tue, 16 Jun 2020 08:14:42: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:14:42: #1 finished! INFO @ Tue, 16 Jun 2020 08:14:42: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:14:42: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:14:42: #2 number of paired peaks: 1059 INFO @ Tue, 16 Jun 2020 08:14:42: start model_add_line... INFO @ Tue, 16 Jun 2020 08:14:42: start X-correlation... INFO @ Tue, 16 Jun 2020 08:14:42: end of X-cor INFO @ Tue, 16 Jun 2020 08:14:42: #2 finished! INFO @ Tue, 16 Jun 2020 08:14:42: #2 predicted fragment length is 169 bps INFO @ Tue, 16 Jun 2020 08:14:42: #2 alternative fragment length(s) may be 169 bps INFO @ Tue, 16 Jun 2020 08:14:42: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX233696/SRX233696.05_model.r INFO @ Tue, 16 Jun 2020 08:14:42: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:14:42: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 08:14:51: #3 Call peaks for each chromosome... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:14:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX233696/SRX233696.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX233696/SRX233696.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX233696/SRX233696.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX233696/SRX233696.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:14:54: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:14:54: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:14:55: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX233696/SRX233696.05_peaks.xls INFO @ Tue, 16 Jun 2020 08:14:55: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX233696/SRX233696.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:14:55: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX233696/SRX233696.05_summits.bed INFO @ Tue, 16 Jun 2020 08:14:55: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (3097 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:15:00: 1000000 INFO @ Tue, 16 Jun 2020 08:15:05: 2000000 INFO @ Tue, 16 Jun 2020 08:15:10: 3000000 INFO @ Tue, 16 Jun 2020 08:15:12: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 08:15:12: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 08:15:12: #1 total tags in treatment: 3345643 INFO @ Tue, 16 Jun 2020 08:15:12: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:15:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:15:12: #1 tags after filtering in treatment: 3345643 INFO @ Tue, 16 Jun 2020 08:15:12: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:15:12: #1 finished! INFO @ Tue, 16 Jun 2020 08:15:12: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:15:12: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:15:13: #2 number of paired peaks: 1059 INFO @ Tue, 16 Jun 2020 08:15:13: start model_add_line... INFO @ Tue, 16 Jun 2020 08:15:13: start X-correlation... INFO @ Tue, 16 Jun 2020 08:15:13: end of X-cor INFO @ Tue, 16 Jun 2020 08:15:13: #2 finished! INFO @ Tue, 16 Jun 2020 08:15:13: #2 predicted fragment length is 169 bps INFO @ Tue, 16 Jun 2020 08:15:13: #2 alternative fragment length(s) may be 169 bps INFO @ Tue, 16 Jun 2020 08:15:13: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX233696/SRX233696.10_model.r INFO @ Tue, 16 Jun 2020 08:15:13: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:15:13: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 08:15:21: #3 Call peaks for each chromosome... BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:15:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX233696/SRX233696.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX233696/SRX233696.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX233696/SRX233696.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX233696/SRX233696.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:15:24: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:15:24: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:15:26: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX233696/SRX233696.10_peaks.xls INFO @ Tue, 16 Jun 2020 08:15:26: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX233696/SRX233696.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:15:26: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX233696/SRX233696.10_summits.bed INFO @ Tue, 16 Jun 2020 08:15:26: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (1585 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:15:30: 1000000 INFO @ Tue, 16 Jun 2020 08:15:35: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 08:15:40: 3000000 INFO @ Tue, 16 Jun 2020 08:15:42: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 08:15:42: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 08:15:42: #1 total tags in treatment: 3345643 INFO @ Tue, 16 Jun 2020 08:15:42: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:15:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:15:42: #1 tags after filtering in treatment: 3345643 INFO @ Tue, 16 Jun 2020 08:15:42: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:15:42: #1 finished! INFO @ Tue, 16 Jun 2020 08:15:42: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:15:42: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:15:42: #2 number of paired peaks: 1059 INFO @ Tue, 16 Jun 2020 08:15:42: start model_add_line... INFO @ Tue, 16 Jun 2020 08:15:42: start X-correlation... INFO @ Tue, 16 Jun 2020 08:15:42: end of X-cor INFO @ Tue, 16 Jun 2020 08:15:42: #2 finished! INFO @ Tue, 16 Jun 2020 08:15:42: #2 predicted fragment length is 169 bps INFO @ Tue, 16 Jun 2020 08:15:42: #2 alternative fragment length(s) may be 169 bps INFO @ Tue, 16 Jun 2020 08:15:42: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX233696/SRX233696.20_model.r INFO @ Tue, 16 Jun 2020 08:15:42: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:15:42: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 08:15:51: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 08:15:55: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX233696/SRX233696.20_peaks.xls INFO @ Tue, 16 Jun 2020 08:15:55: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX233696/SRX233696.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:15:55: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX233696/SRX233696.20_summits.bed INFO @ Tue, 16 Jun 2020 08:15:55: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (666 records, 4 fields): 1 millis CompletedMACS2peakCalling