Job ID = 6366768 SRX = SRX2249334 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T22:59:53 prefetch.2.10.7: 1) Downloading 'SRR4427773'... 2020-06-15T22:59:53 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:12:04 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:12:04 prefetch.2.10.7: 1) 'SRR4427773' was downloaded successfully 2020-06-15T23:12:37 prefetch.2.10.7: 'SRR4427773' has 6 unresolved dependencies 2020-06-15T23:12:37 prefetch.2.10.7: 2) Downloading 'ncbi-acc:BX284601.4?vdb-ctx=refseq'... 2020-06-15T23:12:37 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:12:54 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:12:54 prefetch.2.10.7: 2) 'ncbi-acc:BX284601.4?vdb-ctx=refseq' was downloaded successfully 2020-06-15T23:12:54 prefetch.2.10.7: 3) Downloading 'ncbi-acc:BX284602.4?vdb-ctx=refseq'... 2020-06-15T23:12:54 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:13:10 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:13:10 prefetch.2.10.7: 3) 'ncbi-acc:BX284602.4?vdb-ctx=refseq' was downloaded successfully 2020-06-15T23:13:10 prefetch.2.10.7: 4) Downloading 'ncbi-acc:BX284603.3?vdb-ctx=refseq'... 2020-06-15T23:13:10 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:13:25 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:13:25 prefetch.2.10.7: 4) 'ncbi-acc:BX284603.3?vdb-ctx=refseq' was downloaded successfully 2020-06-15T23:13:25 prefetch.2.10.7: 5) Downloading 'ncbi-acc:BX284604.3?vdb-ctx=refseq'... 2020-06-15T23:13:25 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:13:41 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:13:41 prefetch.2.10.7: 5) 'ncbi-acc:BX284604.3?vdb-ctx=refseq' was downloaded successfully 2020-06-15T23:13:41 prefetch.2.10.7: 6) Downloading 'ncbi-acc:BX284605.4?vdb-ctx=refseq'... 2020-06-15T23:13:41 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:13:57 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:13:57 prefetch.2.10.7: 6) 'ncbi-acc:BX284605.4?vdb-ctx=refseq' was downloaded successfully 2020-06-15T23:13:57 prefetch.2.10.7: 7) Downloading 'ncbi-acc:BX284606.4?vdb-ctx=refseq'... 2020-06-15T23:13:57 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:14:13 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:14:13 prefetch.2.10.7: 7) 'ncbi-acc:BX284606.4?vdb-ctx=refseq' was downloaded successfully Read 162820502 spots for SRR4427773/SRR4427773.sra Written 162820502 spots for SRR4427773/SRR4427773.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:54:33 162820502 reads; of these: 162820502 (100.00%) were unpaired; of these: 21852536 (13.42%) aligned 0 times 113588787 (69.76%) aligned exactly 1 time 27379179 (16.82%) aligned >1 times 86.58% overall alignment rate Time searching: 00:54:33 Overall time: 00:54:33 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 60 files... [bam_rmdupse_core] 62169437 / 140967966 = 0.4410 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:56:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2249334/SRX2249334.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2249334/SRX2249334.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2249334/SRX2249334.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2249334/SRX2249334.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:56:41: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:56:41: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:56:48: 1000000 INFO @ Tue, 16 Jun 2020 09:56:54: 2000000 INFO @ Tue, 16 Jun 2020 09:57:01: 3000000 INFO @ Tue, 16 Jun 2020 09:57:07: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:57:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2249334/SRX2249334.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2249334/SRX2249334.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2249334/SRX2249334.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2249334/SRX2249334.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:57:11: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:57:11: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:57:14: 5000000 INFO @ Tue, 16 Jun 2020 09:57:18: 1000000 INFO @ Tue, 16 Jun 2020 09:57:21: 6000000 INFO @ Tue, 16 Jun 2020 09:57:25: 2000000 INFO @ Tue, 16 Jun 2020 09:57:28: 7000000 INFO @ Tue, 16 Jun 2020 09:57:33: 3000000 INFO @ Tue, 16 Jun 2020 09:57:35: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:57:40: 4000000 INFO @ Tue, 16 Jun 2020 09:57:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2249334/SRX2249334.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2249334/SRX2249334.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2249334/SRX2249334.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2249334/SRX2249334.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:57:41: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:57:41: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:57:42: 9000000 INFO @ Tue, 16 Jun 2020 09:57:48: 5000000 INFO @ Tue, 16 Jun 2020 09:57:49: 1000000 INFO @ Tue, 16 Jun 2020 09:57:50: 10000000 INFO @ Tue, 16 Jun 2020 09:57:55: 6000000 INFO @ Tue, 16 Jun 2020 09:57:57: 11000000 INFO @ Tue, 16 Jun 2020 09:57:58: 2000000 INFO @ Tue, 16 Jun 2020 09:58:03: 7000000 INFO @ Tue, 16 Jun 2020 09:58:05: 12000000 INFO @ Tue, 16 Jun 2020 09:58:06: 3000000 INFO @ Tue, 16 Jun 2020 09:58:10: 8000000 INFO @ Tue, 16 Jun 2020 09:58:12: 13000000 INFO @ Tue, 16 Jun 2020 09:58:14: 4000000 INFO @ Tue, 16 Jun 2020 09:58:18: 9000000 INFO @ Tue, 16 Jun 2020 09:58:20: 14000000 INFO @ Tue, 16 Jun 2020 09:58:22: 5000000 INFO @ Tue, 16 Jun 2020 09:58:25: 10000000 INFO @ Tue, 16 Jun 2020 09:58:27: 15000000 INFO @ Tue, 16 Jun 2020 09:58:30: 6000000 INFO @ Tue, 16 Jun 2020 09:58:33: 11000000 INFO @ Tue, 16 Jun 2020 09:58:35: 16000000 INFO @ Tue, 16 Jun 2020 09:58:39: 7000000 INFO @ Tue, 16 Jun 2020 09:58:40: 12000000 INFO @ Tue, 16 Jun 2020 09:58:42: 17000000 INFO @ Tue, 16 Jun 2020 09:58:47: 8000000 INFO @ Tue, 16 Jun 2020 09:58:48: 13000000 INFO @ Tue, 16 Jun 2020 09:58:50: 18000000 INFO @ Tue, 16 Jun 2020 09:58:55: 9000000 INFO @ Tue, 16 Jun 2020 09:58:55: 14000000 INFO @ Tue, 16 Jun 2020 09:58:58: 19000000 INFO @ Tue, 16 Jun 2020 09:59:03: 10000000 INFO @ Tue, 16 Jun 2020 09:59:03: 15000000 INFO @ Tue, 16 Jun 2020 09:59:05: 20000000 INFO @ Tue, 16 Jun 2020 09:59:11: 11000000 INFO @ Tue, 16 Jun 2020 09:59:11: 16000000 INFO @ Tue, 16 Jun 2020 09:59:13: 21000000 INFO @ Tue, 16 Jun 2020 09:59:18: 17000000 INFO @ Tue, 16 Jun 2020 09:59:19: 12000000 INFO @ Tue, 16 Jun 2020 09:59:20: 22000000 INFO @ Tue, 16 Jun 2020 09:59:26: 18000000 INFO @ Tue, 16 Jun 2020 09:59:27: 13000000 INFO @ Tue, 16 Jun 2020 09:59:28: 23000000 INFO @ Tue, 16 Jun 2020 09:59:33: 19000000 INFO @ Tue, 16 Jun 2020 09:59:35: 14000000 INFO @ Tue, 16 Jun 2020 09:59:36: 24000000 INFO @ Tue, 16 Jun 2020 09:59:41: 20000000 INFO @ Tue, 16 Jun 2020 09:59:43: 25000000 INFO @ Tue, 16 Jun 2020 09:59:44: 15000000 INFO @ Tue, 16 Jun 2020 09:59:49: 21000000 INFO @ Tue, 16 Jun 2020 09:59:51: 26000000 INFO @ Tue, 16 Jun 2020 09:59:52: 16000000 INFO @ Tue, 16 Jun 2020 09:59:56: 22000000 INFO @ Tue, 16 Jun 2020 09:59:58: 27000000 INFO @ Tue, 16 Jun 2020 10:00:00: 17000000 INFO @ Tue, 16 Jun 2020 10:00:04: 23000000 INFO @ Tue, 16 Jun 2020 10:00:06: 28000000 INFO @ Tue, 16 Jun 2020 10:00:08: 18000000 INFO @ Tue, 16 Jun 2020 10:00:12: 24000000 INFO @ Tue, 16 Jun 2020 10:00:14: 29000000 INFO @ Tue, 16 Jun 2020 10:00:16: 19000000 INFO @ Tue, 16 Jun 2020 10:00:19: 25000000 INFO @ Tue, 16 Jun 2020 10:00:21: 30000000 INFO @ Tue, 16 Jun 2020 10:00:24: 20000000 INFO @ Tue, 16 Jun 2020 10:00:27: 26000000 INFO @ Tue, 16 Jun 2020 10:00:29: 31000000 INFO @ Tue, 16 Jun 2020 10:00:32: 21000000 INFO @ Tue, 16 Jun 2020 10:00:35: 27000000 INFO @ Tue, 16 Jun 2020 10:00:37: 32000000 INFO @ Tue, 16 Jun 2020 10:00:41: 22000000 INFO @ Tue, 16 Jun 2020 10:00:43: 28000000 INFO @ Tue, 16 Jun 2020 10:00:44: 33000000 INFO @ Tue, 16 Jun 2020 10:00:49: 23000000 INFO @ Tue, 16 Jun 2020 10:00:50: 29000000 INFO @ Tue, 16 Jun 2020 10:00:52: 34000000 INFO @ Tue, 16 Jun 2020 10:00:58: 24000000 INFO @ Tue, 16 Jun 2020 10:00:58: 30000000 INFO @ Tue, 16 Jun 2020 10:01:00: 35000000 INFO @ Tue, 16 Jun 2020 10:01:06: 31000000 INFO @ Tue, 16 Jun 2020 10:01:06: 25000000 INFO @ Tue, 16 Jun 2020 10:01:08: 36000000 INFO @ Tue, 16 Jun 2020 10:01:13: 32000000 INFO @ Tue, 16 Jun 2020 10:01:15: 26000000 INFO @ Tue, 16 Jun 2020 10:01:15: 37000000 INFO @ Tue, 16 Jun 2020 10:01:21: 33000000 INFO @ Tue, 16 Jun 2020 10:01:23: 38000000 INFO @ Tue, 16 Jun 2020 10:01:24: 27000000 INFO @ Tue, 16 Jun 2020 10:01:29: 34000000 INFO @ Tue, 16 Jun 2020 10:01:31: 39000000 INFO @ Tue, 16 Jun 2020 10:01:32: 28000000 INFO @ Tue, 16 Jun 2020 10:01:36: 35000000 INFO @ Tue, 16 Jun 2020 10:01:38: 40000000 INFO @ Tue, 16 Jun 2020 10:01:41: 29000000 INFO @ Tue, 16 Jun 2020 10:01:44: 36000000 INFO @ Tue, 16 Jun 2020 10:01:46: 41000000 INFO @ Tue, 16 Jun 2020 10:01:49: 30000000 INFO @ Tue, 16 Jun 2020 10:01:52: 37000000 INFO @ Tue, 16 Jun 2020 10:01:54: 42000000 INFO @ Tue, 16 Jun 2020 10:01:58: 31000000 INFO @ Tue, 16 Jun 2020 10:01:59: 38000000 INFO @ Tue, 16 Jun 2020 10:02:01: 43000000 INFO @ Tue, 16 Jun 2020 10:02:07: 32000000 INFO @ Tue, 16 Jun 2020 10:02:07: 39000000 INFO @ Tue, 16 Jun 2020 10:02:09: 44000000 INFO @ Tue, 16 Jun 2020 10:02:15: 40000000 INFO @ Tue, 16 Jun 2020 10:02:15: 33000000 INFO @ Tue, 16 Jun 2020 10:02:17: 45000000 INFO @ Tue, 16 Jun 2020 10:02:22: 41000000 INFO @ Tue, 16 Jun 2020 10:02:24: 34000000 INFO @ Tue, 16 Jun 2020 10:02:24: 46000000 INFO @ Tue, 16 Jun 2020 10:02:30: 42000000 INFO @ Tue, 16 Jun 2020 10:02:32: 47000000 INFO @ Tue, 16 Jun 2020 10:02:32: 35000000 INFO @ Tue, 16 Jun 2020 10:02:38: 43000000 INFO @ Tue, 16 Jun 2020 10:02:40: 48000000 INFO @ Tue, 16 Jun 2020 10:02:41: 36000000 INFO @ Tue, 16 Jun 2020 10:02:45: 44000000 INFO @ Tue, 16 Jun 2020 10:02:47: 49000000 INFO @ Tue, 16 Jun 2020 10:02:50: 37000000 INFO @ Tue, 16 Jun 2020 10:02:53: 45000000 INFO @ Tue, 16 Jun 2020 10:02:55: 50000000 INFO @ Tue, 16 Jun 2020 10:02:58: 38000000 INFO @ Tue, 16 Jun 2020 10:03:00: 46000000 INFO @ Tue, 16 Jun 2020 10:03:02: 51000000 INFO @ Tue, 16 Jun 2020 10:03:07: 39000000 INFO @ Tue, 16 Jun 2020 10:03:08: 47000000 INFO @ Tue, 16 Jun 2020 10:03:10: 52000000 INFO @ Tue, 16 Jun 2020 10:03:15: 40000000 INFO @ Tue, 16 Jun 2020 10:03:15: 48000000 INFO @ Tue, 16 Jun 2020 10:03:17: 53000000 INFO @ Tue, 16 Jun 2020 10:03:23: 49000000 INFO @ Tue, 16 Jun 2020 10:03:24: 41000000 INFO @ Tue, 16 Jun 2020 10:03:25: 54000000 INFO @ Tue, 16 Jun 2020 10:03:30: 50000000 INFO @ Tue, 16 Jun 2020 10:03:32: 42000000 INFO @ Tue, 16 Jun 2020 10:03:32: 55000000 INFO @ Tue, 16 Jun 2020 10:03:38: 51000000 INFO @ Tue, 16 Jun 2020 10:03:40: 56000000 INFO @ Tue, 16 Jun 2020 10:03:40: 43000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 10:03:45: 52000000 INFO @ Tue, 16 Jun 2020 10:03:47: 57000000 INFO @ Tue, 16 Jun 2020 10:03:48: 44000000 INFO @ Tue, 16 Jun 2020 10:03:53: 53000000 INFO @ Tue, 16 Jun 2020 10:03:54: 58000000 INFO @ Tue, 16 Jun 2020 10:03:57: 45000000 INFO @ Tue, 16 Jun 2020 10:04:00: 54000000 INFO @ Tue, 16 Jun 2020 10:04:02: 59000000 INFO @ Tue, 16 Jun 2020 10:04:05: 46000000 INFO @ Tue, 16 Jun 2020 10:04:08: 55000000 INFO @ Tue, 16 Jun 2020 10:04:09: 60000000 INFO @ Tue, 16 Jun 2020 10:04:13: 47000000 INFO @ Tue, 16 Jun 2020 10:04:15: 56000000 INFO @ Tue, 16 Jun 2020 10:04:17: 61000000 INFO @ Tue, 16 Jun 2020 10:04:21: 48000000 INFO @ Tue, 16 Jun 2020 10:04:23: 57000000 INFO @ Tue, 16 Jun 2020 10:04:24: 62000000 INFO @ Tue, 16 Jun 2020 10:04:29: 49000000 INFO @ Tue, 16 Jun 2020 10:04:30: 58000000 INFO @ Tue, 16 Jun 2020 10:04:32: 63000000 INFO @ Tue, 16 Jun 2020 10:04:37: 50000000 INFO @ Tue, 16 Jun 2020 10:04:38: 59000000 INFO @ Tue, 16 Jun 2020 10:04:39: 64000000 INFO @ Tue, 16 Jun 2020 10:04:45: 60000000 INFO @ Tue, 16 Jun 2020 10:04:45: 51000000 INFO @ Tue, 16 Jun 2020 10:04:47: 65000000 INFO @ Tue, 16 Jun 2020 10:04:53: 61000000 INFO @ Tue, 16 Jun 2020 10:04:53: 52000000 INFO @ Tue, 16 Jun 2020 10:04:55: 66000000 INFO @ Tue, 16 Jun 2020 10:05:00: 62000000 INFO @ Tue, 16 Jun 2020 10:05:01: 53000000 INFO @ Tue, 16 Jun 2020 10:05:02: 67000000 INFO @ Tue, 16 Jun 2020 10:05:08: 63000000 INFO @ Tue, 16 Jun 2020 10:05:09: 54000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 10:05:10: 68000000 INFO @ Tue, 16 Jun 2020 10:05:16: 64000000 INFO @ Tue, 16 Jun 2020 10:05:17: 55000000 INFO @ Tue, 16 Jun 2020 10:05:18: 69000000 INFO @ Tue, 16 Jun 2020 10:05:24: 65000000 INFO @ Tue, 16 Jun 2020 10:05:25: 56000000 INFO @ Tue, 16 Jun 2020 10:05:25: 70000000 INFO @ Tue, 16 Jun 2020 10:05:31: 66000000 INFO @ Tue, 16 Jun 2020 10:05:33: 57000000 INFO @ Tue, 16 Jun 2020 10:05:33: 71000000 INFO @ Tue, 16 Jun 2020 10:05:39: 67000000 INFO @ Tue, 16 Jun 2020 10:05:40: 72000000 INFO @ Tue, 16 Jun 2020 10:05:41: 58000000 INFO @ Tue, 16 Jun 2020 10:05:47: 68000000 INFO @ Tue, 16 Jun 2020 10:05:48: 73000000 INFO @ Tue, 16 Jun 2020 10:05:49: 59000000 INFO @ Tue, 16 Jun 2020 10:05:54: 69000000 INFO @ Tue, 16 Jun 2020 10:05:56: 74000000 INFO @ Tue, 16 Jun 2020 10:05:57: 60000000 INFO @ Tue, 16 Jun 2020 10:06:02: 70000000 INFO @ Tue, 16 Jun 2020 10:06:03: 75000000 INFO @ Tue, 16 Jun 2020 10:06:05: 61000000 INFO @ Tue, 16 Jun 2020 10:06:10: 71000000 INFO @ Tue, 16 Jun 2020 10:06:11: 76000000 INFO @ Tue, 16 Jun 2020 10:06:13: 62000000 INFO @ Tue, 16 Jun 2020 10:06:17: 72000000 INFO @ Tue, 16 Jun 2020 10:06:19: 77000000 INFO @ Tue, 16 Jun 2020 10:06:21: 63000000 INFO @ Tue, 16 Jun 2020 10:06:25: 73000000 INFO @ Tue, 16 Jun 2020 10:06:26: 78000000 INFO @ Tue, 16 Jun 2020 10:06:29: 64000000 INFO @ Tue, 16 Jun 2020 10:06:32: 74000000 INFO @ Tue, 16 Jun 2020 10:06:33: #1 tag size is determined as 101 bps INFO @ Tue, 16 Jun 2020 10:06:33: #1 tag size = 101 INFO @ Tue, 16 Jun 2020 10:06:33: #1 total tags in treatment: 78798529 INFO @ Tue, 16 Jun 2020 10:06:33: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:06:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:06:34: #1 tags after filtering in treatment: 78798529 INFO @ Tue, 16 Jun 2020 10:06:34: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:06:34: #1 finished! INFO @ Tue, 16 Jun 2020 10:06:34: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:06:34: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:06:37: 65000000 INFO @ Tue, 16 Jun 2020 10:06:39: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 10:06:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:06:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2249334/SRX2249334.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2249334/SRX2249334.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2249334/SRX2249334.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2249334/SRX2249334.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:06:40: 75000000 INFO @ Tue, 16 Jun 2020 10:06:46: 66000000 INFO @ Tue, 16 Jun 2020 10:06:48: 76000000 INFO @ Tue, 16 Jun 2020 10:06:54: 67000000 INFO @ Tue, 16 Jun 2020 10:06:56: 77000000 INFO @ Tue, 16 Jun 2020 10:07:02: 68000000 INFO @ Tue, 16 Jun 2020 10:07:04: 78000000 INFO @ Tue, 16 Jun 2020 10:07:10: 69000000 INFO @ Tue, 16 Jun 2020 10:07:11: #1 tag size is determined as 101 bps INFO @ Tue, 16 Jun 2020 10:07:11: #1 tag size = 101 INFO @ Tue, 16 Jun 2020 10:07:11: #1 total tags in treatment: 78798529 INFO @ Tue, 16 Jun 2020 10:07:11: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:07:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:07:12: #1 tags after filtering in treatment: 78798529 INFO @ Tue, 16 Jun 2020 10:07:12: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:07:12: #1 finished! INFO @ Tue, 16 Jun 2020 10:07:12: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:07:12: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:07:17: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 10:07:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:07:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2249334/SRX2249334.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2249334/SRX2249334.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2249334/SRX2249334.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2249334/SRX2249334.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:07:18: 70000000 INFO @ Tue, 16 Jun 2020 10:07:26: 71000000 INFO @ Tue, 16 Jun 2020 10:07:34: 72000000 INFO @ Tue, 16 Jun 2020 10:07:41: 73000000 INFO @ Tue, 16 Jun 2020 10:07:49: 74000000 INFO @ Tue, 16 Jun 2020 10:07:56: 75000000 INFO @ Tue, 16 Jun 2020 10:08:04: 76000000 INFO @ Tue, 16 Jun 2020 10:08:11: 77000000 INFO @ Tue, 16 Jun 2020 10:08:19: 78000000 INFO @ Tue, 16 Jun 2020 10:08:26: #1 tag size is determined as 101 bps INFO @ Tue, 16 Jun 2020 10:08:26: #1 tag size = 101 INFO @ Tue, 16 Jun 2020 10:08:26: #1 total tags in treatment: 78798529 INFO @ Tue, 16 Jun 2020 10:08:26: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:08:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:08:27: #1 tags after filtering in treatment: 78798529 INFO @ Tue, 16 Jun 2020 10:08:27: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:08:27: #1 finished! INFO @ Tue, 16 Jun 2020 10:08:27: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:08:27: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:08:32: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 10:08:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:08:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2249334/SRX2249334.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2249334/SRX2249334.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2249334/SRX2249334.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2249334/SRX2249334.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling