Job ID = 6366677 SRX = SRX2202789 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T22:57:53 prefetch.2.10.7: 1) Downloading 'SRR4319295'... 2020-06-15T22:57:53 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:00:55 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:00:55 prefetch.2.10.7: 1) 'SRR4319295' was downloaded successfully 2020-06-15T23:01:27 prefetch.2.10.7: 'SRR4319295' has 6 unresolved dependencies 2020-06-15T23:01:27 prefetch.2.10.7: 2) Downloading 'ncbi-acc:BX284601.4?vdb-ctx=refseq'... 2020-06-15T23:01:27 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:01:46 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:01:46 prefetch.2.10.7: 2) 'ncbi-acc:BX284601.4?vdb-ctx=refseq' was downloaded successfully 2020-06-15T23:01:46 prefetch.2.10.7: 3) Downloading 'ncbi-acc:BX284602.4?vdb-ctx=refseq'... 2020-06-15T23:01:46 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:02:01 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:02:01 prefetch.2.10.7: 3) 'ncbi-acc:BX284602.4?vdb-ctx=refseq' was downloaded successfully 2020-06-15T23:02:01 prefetch.2.10.7: 4) Downloading 'ncbi-acc:BX284603.3?vdb-ctx=refseq'... 2020-06-15T23:02:01 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:02:16 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:02:16 prefetch.2.10.7: 4) 'ncbi-acc:BX284603.3?vdb-ctx=refseq' was downloaded successfully 2020-06-15T23:02:16 prefetch.2.10.7: 5) Downloading 'ncbi-acc:BX284604.3?vdb-ctx=refseq'... 2020-06-15T23:02:16 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:02:33 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:02:33 prefetch.2.10.7: 5) 'ncbi-acc:BX284604.3?vdb-ctx=refseq' was downloaded successfully 2020-06-15T23:02:33 prefetch.2.10.7: 6) Downloading 'ncbi-acc:BX284605.4?vdb-ctx=refseq'... 2020-06-15T23:02:33 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:02:48 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:02:48 prefetch.2.10.7: 6) 'ncbi-acc:BX284605.4?vdb-ctx=refseq' was downloaded successfully 2020-06-15T23:02:48 prefetch.2.10.7: 7) Downloading 'ncbi-acc:BX284606.4?vdb-ctx=refseq'... 2020-06-15T23:02:48 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:03:03 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:03:03 prefetch.2.10.7: 7) 'ncbi-acc:BX284606.4?vdb-ctx=refseq' was downloaded successfully Read 80110862 spots for SRR4319295/SRR4319295.sra Written 80110862 spots for SRR4319295/SRR4319295.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:40 80110862 reads; of these: 80110862 (100.00%) were unpaired; of these: 2163532 (2.70%) aligned 0 times 64906684 (81.02%) aligned exactly 1 time 13040646 (16.28%) aligned >1 times 97.30% overall alignment rate Time searching: 00:11:40 Overall time: 00:11:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 32406380 / 77947330 = 0.4157 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:33:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2202789/SRX2202789.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2202789/SRX2202789.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2202789/SRX2202789.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2202789/SRX2202789.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:33:08: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:33:08: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:33:14: 1000000 INFO @ Tue, 16 Jun 2020 08:33:20: 2000000 INFO @ Tue, 16 Jun 2020 08:33:26: 3000000 INFO @ Tue, 16 Jun 2020 08:33:32: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:33:38: 5000000 INFO @ Tue, 16 Jun 2020 08:33:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2202789/SRX2202789.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2202789/SRX2202789.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2202789/SRX2202789.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2202789/SRX2202789.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:33:38: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:33:38: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:33:44: 6000000 INFO @ Tue, 16 Jun 2020 08:33:45: 1000000 INFO @ Tue, 16 Jun 2020 08:33:51: 7000000 INFO @ Tue, 16 Jun 2020 08:33:52: 2000000 INFO @ Tue, 16 Jun 2020 08:33:58: 8000000 INFO @ Tue, 16 Jun 2020 08:33:59: 3000000 INFO @ Tue, 16 Jun 2020 08:34:05: 9000000 INFO @ Tue, 16 Jun 2020 08:34:06: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:34:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2202789/SRX2202789.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2202789/SRX2202789.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2202789/SRX2202789.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2202789/SRX2202789.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:34:08: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:34:08: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:34:11: 10000000 INFO @ Tue, 16 Jun 2020 08:34:13: 5000000 INFO @ Tue, 16 Jun 2020 08:34:15: 1000000 INFO @ Tue, 16 Jun 2020 08:34:18: 11000000 INFO @ Tue, 16 Jun 2020 08:34:20: 6000000 INFO @ Tue, 16 Jun 2020 08:34:22: 2000000 INFO @ Tue, 16 Jun 2020 08:34:25: 12000000 INFO @ Tue, 16 Jun 2020 08:34:27: 7000000 INFO @ Tue, 16 Jun 2020 08:34:29: 3000000 INFO @ Tue, 16 Jun 2020 08:34:32: 13000000 INFO @ Tue, 16 Jun 2020 08:34:34: 8000000 INFO @ Tue, 16 Jun 2020 08:34:36: 4000000 INFO @ Tue, 16 Jun 2020 08:34:39: 14000000 INFO @ Tue, 16 Jun 2020 08:34:41: 9000000 INFO @ Tue, 16 Jun 2020 08:34:42: 5000000 INFO @ Tue, 16 Jun 2020 08:34:46: 15000000 INFO @ Tue, 16 Jun 2020 08:34:47: 10000000 INFO @ Tue, 16 Jun 2020 08:34:49: 6000000 INFO @ Tue, 16 Jun 2020 08:34:52: 16000000 INFO @ Tue, 16 Jun 2020 08:34:54: 11000000 INFO @ Tue, 16 Jun 2020 08:34:56: 7000000 INFO @ Tue, 16 Jun 2020 08:34:59: 17000000 INFO @ Tue, 16 Jun 2020 08:35:01: 12000000 INFO @ Tue, 16 Jun 2020 08:35:03: 8000000 INFO @ Tue, 16 Jun 2020 08:35:06: 18000000 INFO @ Tue, 16 Jun 2020 08:35:08: 13000000 INFO @ Tue, 16 Jun 2020 08:35:10: 9000000 INFO @ Tue, 16 Jun 2020 08:35:13: 19000000 INFO @ Tue, 16 Jun 2020 08:35:15: 14000000 INFO @ Tue, 16 Jun 2020 08:35:17: 10000000 INFO @ Tue, 16 Jun 2020 08:35:20: 20000000 INFO @ Tue, 16 Jun 2020 08:35:22: 15000000 INFO @ Tue, 16 Jun 2020 08:35:24: 11000000 INFO @ Tue, 16 Jun 2020 08:35:26: 21000000 INFO @ Tue, 16 Jun 2020 08:35:29: 16000000 INFO @ Tue, 16 Jun 2020 08:35:30: 12000000 INFO @ Tue, 16 Jun 2020 08:35:33: 22000000 INFO @ Tue, 16 Jun 2020 08:35:36: 17000000 INFO @ Tue, 16 Jun 2020 08:35:37: 13000000 INFO @ Tue, 16 Jun 2020 08:35:40: 23000000 INFO @ Tue, 16 Jun 2020 08:35:42: 18000000 INFO @ Tue, 16 Jun 2020 08:35:44: 14000000 INFO @ Tue, 16 Jun 2020 08:35:46: 24000000 INFO @ Tue, 16 Jun 2020 08:35:49: 19000000 INFO @ Tue, 16 Jun 2020 08:35:51: 15000000 INFO @ Tue, 16 Jun 2020 08:35:53: 25000000 INFO @ Tue, 16 Jun 2020 08:35:56: 20000000 INFO @ Tue, 16 Jun 2020 08:35:58: 16000000 INFO @ Tue, 16 Jun 2020 08:36:00: 26000000 INFO @ Tue, 16 Jun 2020 08:36:03: 21000000 INFO @ Tue, 16 Jun 2020 08:36:05: 17000000 INFO @ Tue, 16 Jun 2020 08:36:06: 27000000 INFO @ Tue, 16 Jun 2020 08:36:10: 22000000 INFO @ Tue, 16 Jun 2020 08:36:11: 18000000 INFO @ Tue, 16 Jun 2020 08:36:13: 28000000 INFO @ Tue, 16 Jun 2020 08:36:16: 23000000 INFO @ Tue, 16 Jun 2020 08:36:18: 19000000 INFO @ Tue, 16 Jun 2020 08:36:19: 29000000 INFO @ Tue, 16 Jun 2020 08:36:23: 24000000 INFO @ Tue, 16 Jun 2020 08:36:24: 20000000 INFO @ Tue, 16 Jun 2020 08:36:26: 30000000 INFO @ Tue, 16 Jun 2020 08:36:29: 25000000 INFO @ Tue, 16 Jun 2020 08:36:31: 21000000 INFO @ Tue, 16 Jun 2020 08:36:32: 31000000 INFO @ Tue, 16 Jun 2020 08:36:36: 26000000 INFO @ Tue, 16 Jun 2020 08:36:38: 22000000 INFO @ Tue, 16 Jun 2020 08:36:39: 32000000 INFO @ Tue, 16 Jun 2020 08:36:43: 27000000 INFO @ Tue, 16 Jun 2020 08:36:44: 23000000 INFO @ Tue, 16 Jun 2020 08:36:45: 33000000 INFO @ Tue, 16 Jun 2020 08:36:49: 28000000 INFO @ Tue, 16 Jun 2020 08:36:51: 24000000 INFO @ Tue, 16 Jun 2020 08:36:52: 34000000 INFO @ Tue, 16 Jun 2020 08:36:56: 29000000 INFO @ Tue, 16 Jun 2020 08:36:57: 25000000 INFO @ Tue, 16 Jun 2020 08:36:58: 35000000 INFO @ Tue, 16 Jun 2020 08:37:03: 30000000 INFO @ Tue, 16 Jun 2020 08:37:04: 26000000 INFO @ Tue, 16 Jun 2020 08:37:05: 36000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 08:37:10: 31000000 INFO @ Tue, 16 Jun 2020 08:37:11: 27000000 INFO @ Tue, 16 Jun 2020 08:37:11: 37000000 INFO @ Tue, 16 Jun 2020 08:37:16: 32000000 INFO @ Tue, 16 Jun 2020 08:37:17: 28000000 INFO @ Tue, 16 Jun 2020 08:37:18: 38000000 INFO @ Tue, 16 Jun 2020 08:37:23: 33000000 INFO @ Tue, 16 Jun 2020 08:37:24: 29000000 INFO @ Tue, 16 Jun 2020 08:37:25: 39000000 INFO @ Tue, 16 Jun 2020 08:37:29: 34000000 INFO @ Tue, 16 Jun 2020 08:37:30: 30000000 INFO @ Tue, 16 Jun 2020 08:37:31: 40000000 INFO @ Tue, 16 Jun 2020 08:37:36: 35000000 INFO @ Tue, 16 Jun 2020 08:37:37: 31000000 INFO @ Tue, 16 Jun 2020 08:37:38: 41000000 INFO @ Tue, 16 Jun 2020 08:37:43: 36000000 INFO @ Tue, 16 Jun 2020 08:37:44: 32000000 INFO @ Tue, 16 Jun 2020 08:37:45: 42000000 INFO @ Tue, 16 Jun 2020 08:37:50: 37000000 INFO @ Tue, 16 Jun 2020 08:37:50: 33000000 INFO @ Tue, 16 Jun 2020 08:37:52: 43000000 INFO @ Tue, 16 Jun 2020 08:37:56: 38000000 INFO @ Tue, 16 Jun 2020 08:37:57: 34000000 INFO @ Tue, 16 Jun 2020 08:37:58: 44000000 INFO @ Tue, 16 Jun 2020 08:38:03: 39000000 INFO @ Tue, 16 Jun 2020 08:38:04: 35000000 INFO @ Tue, 16 Jun 2020 08:38:05: 45000000 INFO @ Tue, 16 Jun 2020 08:38:09: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 08:38:09: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 08:38:09: #1 total tags in treatment: 45540950 INFO @ Tue, 16 Jun 2020 08:38:09: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:38:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:38:10: #1 tags after filtering in treatment: 45540950 INFO @ Tue, 16 Jun 2020 08:38:10: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:38:10: #1 finished! INFO @ Tue, 16 Jun 2020 08:38:10: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:38:10: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:38:10: 40000000 INFO @ Tue, 16 Jun 2020 08:38:10: 36000000 INFO @ Tue, 16 Jun 2020 08:38:13: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 08:38:13: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:38:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2202789/SRX2202789.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2202789/SRX2202789.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2202789/SRX2202789.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2202789/SRX2202789.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:38:17: 41000000 INFO @ Tue, 16 Jun 2020 08:38:17: 37000000 INFO @ Tue, 16 Jun 2020 08:38:24: 42000000 INFO @ Tue, 16 Jun 2020 08:38:24: 38000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 08:38:30: 39000000 INFO @ Tue, 16 Jun 2020 08:38:30: 43000000 INFO @ Tue, 16 Jun 2020 08:38:37: 40000000 INFO @ Tue, 16 Jun 2020 08:38:38: 44000000 INFO @ Tue, 16 Jun 2020 08:38:44: 41000000 INFO @ Tue, 16 Jun 2020 08:38:45: 45000000 INFO @ Tue, 16 Jun 2020 08:38:49: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 08:38:49: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 08:38:49: #1 total tags in treatment: 45540950 INFO @ Tue, 16 Jun 2020 08:38:49: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:38:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:38:50: #1 tags after filtering in treatment: 45540950 INFO @ Tue, 16 Jun 2020 08:38:50: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:38:50: #1 finished! INFO @ Tue, 16 Jun 2020 08:38:50: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:38:50: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:38:50: 42000000 INFO @ Tue, 16 Jun 2020 08:38:53: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 08:38:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:38:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2202789/SRX2202789.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2202789/SRX2202789.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2202789/SRX2202789.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2202789/SRX2202789.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:38:56: 43000000 INFO @ Tue, 16 Jun 2020 08:39:02: 44000000 INFO @ Tue, 16 Jun 2020 08:39:08: 45000000 INFO @ Tue, 16 Jun 2020 08:39:12: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 08:39:12: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 08:39:12: #1 total tags in treatment: 45540950 INFO @ Tue, 16 Jun 2020 08:39:12: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:39:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:39:12: #1 tags after filtering in treatment: 45540950 INFO @ Tue, 16 Jun 2020 08:39:12: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:39:12: #1 finished! INFO @ Tue, 16 Jun 2020 08:39:12: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:39:12: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:39:15: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 08:39:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:39:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2202789/SRX2202789.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2202789/SRX2202789.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2202789/SRX2202789.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2202789/SRX2202789.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling