Job ID = 6366653 SRX = SRX216729 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T22:53:26 prefetch.2.10.7: 1) Downloading 'SRR648332'... 2020-06-15T22:53:26 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T22:54:04 prefetch.2.10.7: HTTPS download succeed 2020-06-15T22:54:05 prefetch.2.10.7: 'SRR648332' is valid 2020-06-15T22:54:05 prefetch.2.10.7: 1) 'SRR648332' was downloaded successfully Read 6691179 spots for SRR648332/SRR648332.sra Written 6691179 spots for SRR648332/SRR648332.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:32 6691179 reads; of these: 6691179 (100.00%) were unpaired; of these: 697329 (10.42%) aligned 0 times 5103815 (76.28%) aligned exactly 1 time 890035 (13.30%) aligned >1 times 89.58% overall alignment rate Time searching: 00:01:32 Overall time: 00:01:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 323254 / 5993850 = 0.0539 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:58:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX216729/SRX216729.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX216729/SRX216729.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX216729/SRX216729.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX216729/SRX216729.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:58:18: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:58:18: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:58:25: 1000000 INFO @ Tue, 16 Jun 2020 07:58:32: 2000000 INFO @ Tue, 16 Jun 2020 07:58:40: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:58:47: 4000000 INFO @ Tue, 16 Jun 2020 07:58:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX216729/SRX216729.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX216729/SRX216729.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX216729/SRX216729.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX216729/SRX216729.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:58:48: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:58:48: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:58:55: 1000000 INFO @ Tue, 16 Jun 2020 07:58:56: 5000000 INFO @ Tue, 16 Jun 2020 07:59:01: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 07:59:01: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 07:59:01: #1 total tags in treatment: 5670596 INFO @ Tue, 16 Jun 2020 07:59:01: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:59:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:59:01: #1 tags after filtering in treatment: 5670596 INFO @ Tue, 16 Jun 2020 07:59:01: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:59:01: #1 finished! INFO @ Tue, 16 Jun 2020 07:59:01: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:59:01: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:59:01: #2 number of paired peaks: 283 WARNING @ Tue, 16 Jun 2020 07:59:01: Fewer paired peaks (283) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 283 pairs to build model! INFO @ Tue, 16 Jun 2020 07:59:01: start model_add_line... INFO @ Tue, 16 Jun 2020 07:59:01: start X-correlation... INFO @ Tue, 16 Jun 2020 07:59:01: end of X-cor INFO @ Tue, 16 Jun 2020 07:59:01: #2 finished! INFO @ Tue, 16 Jun 2020 07:59:01: #2 predicted fragment length is 51 bps INFO @ Tue, 16 Jun 2020 07:59:01: #2 alternative fragment length(s) may be 4,51 bps INFO @ Tue, 16 Jun 2020 07:59:01: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX216729/SRX216729.05_model.r WARNING @ Tue, 16 Jun 2020 07:59:01: #2 Since the d (51) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 07:59:01: #2 You may need to consider one of the other alternative d(s): 4,51 WARNING @ Tue, 16 Jun 2020 07:59:01: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 07:59:01: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:59:01: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:59:03: 2000000 INFO @ Tue, 16 Jun 2020 07:59:10: 3000000 INFO @ Tue, 16 Jun 2020 07:59:14: #3 Call peaks for each chromosome... BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:59:17: 4000000 INFO @ Tue, 16 Jun 2020 07:59:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX216729/SRX216729.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX216729/SRX216729.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX216729/SRX216729.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX216729/SRX216729.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:59:18: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:59:18: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:59:20: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX216729/SRX216729.05_peaks.xls INFO @ Tue, 16 Jun 2020 07:59:20: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX216729/SRX216729.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:59:20: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX216729/SRX216729.05_summits.bed INFO @ Tue, 16 Jun 2020 07:59:20: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (416 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 07:59:24: 5000000 INFO @ Tue, 16 Jun 2020 07:59:25: 1000000 INFO @ Tue, 16 Jun 2020 07:59:29: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 07:59:29: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 07:59:29: #1 total tags in treatment: 5670596 INFO @ Tue, 16 Jun 2020 07:59:29: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:59:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:59:29: #1 tags after filtering in treatment: 5670596 INFO @ Tue, 16 Jun 2020 07:59:29: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:59:29: #1 finished! INFO @ Tue, 16 Jun 2020 07:59:29: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:59:29: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:59:30: #2 number of paired peaks: 283 WARNING @ Tue, 16 Jun 2020 07:59:30: Fewer paired peaks (283) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 283 pairs to build model! INFO @ Tue, 16 Jun 2020 07:59:30: start model_add_line... INFO @ Tue, 16 Jun 2020 07:59:30: start X-correlation... INFO @ Tue, 16 Jun 2020 07:59:30: end of X-cor INFO @ Tue, 16 Jun 2020 07:59:30: #2 finished! INFO @ Tue, 16 Jun 2020 07:59:30: #2 predicted fragment length is 51 bps INFO @ Tue, 16 Jun 2020 07:59:30: #2 alternative fragment length(s) may be 4,51 bps INFO @ Tue, 16 Jun 2020 07:59:30: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX216729/SRX216729.10_model.r WARNING @ Tue, 16 Jun 2020 07:59:30: #2 Since the d (51) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 07:59:30: #2 You may need to consider one of the other alternative d(s): 4,51 WARNING @ Tue, 16 Jun 2020 07:59:30: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 07:59:30: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:59:30: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:59:33: 2000000 INFO @ Tue, 16 Jun 2020 07:59:40: 3000000 INFO @ Tue, 16 Jun 2020 07:59:43: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:59:46: 4000000 INFO @ Tue, 16 Jun 2020 07:59:49: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX216729/SRX216729.10_peaks.xls INFO @ Tue, 16 Jun 2020 07:59:49: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX216729/SRX216729.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:59:49: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX216729/SRX216729.10_summits.bed INFO @ Tue, 16 Jun 2020 07:59:49: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (236 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 07:59:53: 5000000 INFO @ Tue, 16 Jun 2020 07:59:58: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 07:59:58: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 07:59:58: #1 total tags in treatment: 5670596 INFO @ Tue, 16 Jun 2020 07:59:58: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:59:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:59:58: #1 tags after filtering in treatment: 5670596 INFO @ Tue, 16 Jun 2020 07:59:58: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:59:58: #1 finished! INFO @ Tue, 16 Jun 2020 07:59:58: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:59:58: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:59:58: #2 number of paired peaks: 283 WARNING @ Tue, 16 Jun 2020 07:59:58: Fewer paired peaks (283) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 283 pairs to build model! INFO @ Tue, 16 Jun 2020 07:59:58: start model_add_line... INFO @ Tue, 16 Jun 2020 07:59:58: start X-correlation... INFO @ Tue, 16 Jun 2020 07:59:58: end of X-cor INFO @ Tue, 16 Jun 2020 07:59:58: #2 finished! INFO @ Tue, 16 Jun 2020 07:59:58: #2 predicted fragment length is 51 bps INFO @ Tue, 16 Jun 2020 07:59:58: #2 alternative fragment length(s) may be 4,51 bps INFO @ Tue, 16 Jun 2020 07:59:58: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX216729/SRX216729.20_model.r WARNING @ Tue, 16 Jun 2020 07:59:58: #2 Since the d (51) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 07:59:58: #2 You may need to consider one of the other alternative d(s): 4,51 WARNING @ Tue, 16 Jun 2020 07:59:58: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 07:59:58: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:59:58: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 08:00:11: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 08:00:17: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX216729/SRX216729.20_peaks.xls INFO @ Tue, 16 Jun 2020 08:00:17: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX216729/SRX216729.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:00:17: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX216729/SRX216729.20_summits.bed INFO @ Tue, 16 Jun 2020 08:00:17: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (79 records, 4 fields): 1 millis CompletedMACS2peakCalling