Job ID = 6507732 SRX = SRX1674103 Genome = ce11 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-06-26T13:00:56 prefetch.2.10.7: 1) Downloading 'SRR3320149'... 2020-06-26T13:00:56 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T13:15:52 prefetch.2.10.7: HTTPS download succeed 2020-06-26T13:15:52 prefetch.2.10.7: 1) 'SRR3320149' was downloaded successfully 2020-06-26T13:15:52 prefetch.2.10.7: 'SRR3320149' has 0 unresolved dependencies Read 39983162 spots for SRR3320149/SRR3320149.sra Written 39983162 spots for SRR3320149/SRR3320149.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:10:15 39983162 reads; of these: 39983162 (100.00%) were paired; of these: 847908 (2.12%) aligned concordantly 0 times 33613157 (84.07%) aligned concordantly exactly 1 time 5522097 (13.81%) aligned concordantly >1 times ---- 847908 pairs aligned concordantly 0 times; of these: 64180 (7.57%) aligned discordantly 1 time ---- 783728 pairs aligned 0 times concordantly or discordantly; of these: 1567456 mates make up the pairs; of these: 967346 (61.71%) aligned 0 times 438994 (28.01%) aligned exactly 1 time 161116 (10.28%) aligned >1 times 98.79% overall alignment rate Time searching: 01:10:15 Overall time: 01:10:15 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 36 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 6240122 / 39153713 = 0.1594 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:03:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1674103/SRX1674103.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1674103/SRX1674103.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1674103/SRX1674103.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1674103/SRX1674103.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:03:45: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:03:45: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:03:52: 1000000 INFO @ Sat, 27 Jun 2020 00:03:58: 2000000 INFO @ Sat, 27 Jun 2020 00:04:04: 3000000 INFO @ Sat, 27 Jun 2020 00:04:11: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:04:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1674103/SRX1674103.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1674103/SRX1674103.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1674103/SRX1674103.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1674103/SRX1674103.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:04:15: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:04:15: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:04:17: 5000000 INFO @ Sat, 27 Jun 2020 00:04:22: 1000000 INFO @ Sat, 27 Jun 2020 00:04:24: 6000000 INFO @ Sat, 27 Jun 2020 00:04:28: 2000000 INFO @ Sat, 27 Jun 2020 00:04:31: 7000000 INFO @ Sat, 27 Jun 2020 00:04:35: 3000000 INFO @ Sat, 27 Jun 2020 00:04:38: 8000000 INFO @ Sat, 27 Jun 2020 00:04:42: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 27 Jun 2020 00:04:45: 9000000 INFO @ Sat, 27 Jun 2020 00:04:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1674103/SRX1674103.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1674103/SRX1674103.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1674103/SRX1674103.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1674103/SRX1674103.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 27 Jun 2020 00:04:45: #1 read tag files... INFO @ Sat, 27 Jun 2020 00:04:45: #1 read treatment tags... INFO @ Sat, 27 Jun 2020 00:04:49: 5000000 INFO @ Sat, 27 Jun 2020 00:04:52: 10000000 INFO @ Sat, 27 Jun 2020 00:04:54: 1000000 INFO @ Sat, 27 Jun 2020 00:04:56: 6000000 INFO @ Sat, 27 Jun 2020 00:04:59: 11000000 INFO @ Sat, 27 Jun 2020 00:05:02: 2000000 INFO @ Sat, 27 Jun 2020 00:05:03: 7000000 INFO @ Sat, 27 Jun 2020 00:05:06: 12000000 INFO @ Sat, 27 Jun 2020 00:05:10: 3000000 INFO @ Sat, 27 Jun 2020 00:05:10: 8000000 INFO @ Sat, 27 Jun 2020 00:05:13: 13000000 INFO @ Sat, 27 Jun 2020 00:05:18: 9000000 INFO @ Sat, 27 Jun 2020 00:05:19: 4000000 INFO @ Sat, 27 Jun 2020 00:05:21: 14000000 INFO @ Sat, 27 Jun 2020 00:05:25: 10000000 INFO @ Sat, 27 Jun 2020 00:05:27: 5000000 INFO @ Sat, 27 Jun 2020 00:05:28: 15000000 INFO @ Sat, 27 Jun 2020 00:05:32: 11000000 INFO @ Sat, 27 Jun 2020 00:05:35: 16000000 INFO @ Sat, 27 Jun 2020 00:05:36: 6000000 INFO @ Sat, 27 Jun 2020 00:05:39: 12000000 INFO @ Sat, 27 Jun 2020 00:05:42: 17000000 INFO @ Sat, 27 Jun 2020 00:05:44: 7000000 INFO @ Sat, 27 Jun 2020 00:05:46: 13000000 INFO @ Sat, 27 Jun 2020 00:05:49: 18000000 INFO @ Sat, 27 Jun 2020 00:05:53: 8000000 INFO @ Sat, 27 Jun 2020 00:05:53: 14000000 INFO @ Sat, 27 Jun 2020 00:05:56: 19000000 INFO @ Sat, 27 Jun 2020 00:06:00: 15000000 INFO @ Sat, 27 Jun 2020 00:06:01: 9000000 INFO @ Sat, 27 Jun 2020 00:06:03: 20000000 INFO @ Sat, 27 Jun 2020 00:06:08: 16000000 INFO @ Sat, 27 Jun 2020 00:06:09: 10000000 INFO @ Sat, 27 Jun 2020 00:06:11: 21000000 INFO @ Sat, 27 Jun 2020 00:06:15: 17000000 INFO @ Sat, 27 Jun 2020 00:06:17: 11000000 INFO @ Sat, 27 Jun 2020 00:06:18: 22000000 INFO @ Sat, 27 Jun 2020 00:06:22: 18000000 INFO @ Sat, 27 Jun 2020 00:06:25: 23000000 INFO @ Sat, 27 Jun 2020 00:06:26: 12000000 INFO @ Sat, 27 Jun 2020 00:06:29: 19000000 INFO @ Sat, 27 Jun 2020 00:06:32: 24000000 INFO @ Sat, 27 Jun 2020 00:06:34: 13000000 INFO @ Sat, 27 Jun 2020 00:06:36: 20000000 INFO @ Sat, 27 Jun 2020 00:06:39: 25000000 INFO @ Sat, 27 Jun 2020 00:06:42: 14000000 INFO @ Sat, 27 Jun 2020 00:06:43: 21000000 INFO @ Sat, 27 Jun 2020 00:06:46: 26000000 INFO @ Sat, 27 Jun 2020 00:06:50: 22000000 INFO @ Sat, 27 Jun 2020 00:06:50: 15000000 INFO @ Sat, 27 Jun 2020 00:06:52: 27000000 INFO @ Sat, 27 Jun 2020 00:06:57: 23000000 INFO @ Sat, 27 Jun 2020 00:06:58: 16000000 INFO @ Sat, 27 Jun 2020 00:06:59: 28000000 INFO @ Sat, 27 Jun 2020 00:07:04: 24000000 INFO @ Sat, 27 Jun 2020 00:07:05: 29000000 INFO @ Sat, 27 Jun 2020 00:07:06: 17000000 INFO @ Sat, 27 Jun 2020 00:07:11: 25000000 INFO @ Sat, 27 Jun 2020 00:07:12: 30000000 INFO @ Sat, 27 Jun 2020 00:07:14: 18000000 INFO @ Sat, 27 Jun 2020 00:07:17: 26000000 INFO @ Sat, 27 Jun 2020 00:07:19: 31000000 INFO @ Sat, 27 Jun 2020 00:07:22: 19000000 INFO @ Sat, 27 Jun 2020 00:07:24: 27000000 INFO @ Sat, 27 Jun 2020 00:07:26: 32000000 INFO @ Sat, 27 Jun 2020 00:07:30: 20000000 INFO @ Sat, 27 Jun 2020 00:07:30: 28000000 INFO @ Sat, 27 Jun 2020 00:07:33: 33000000 INFO @ Sat, 27 Jun 2020 00:07:37: 29000000 INFO @ Sat, 27 Jun 2020 00:07:37: 21000000 INFO @ Sat, 27 Jun 2020 00:07:39: 34000000 INFO @ Sat, 27 Jun 2020 00:07:43: 30000000 INFO @ Sat, 27 Jun 2020 00:07:45: 22000000 INFO @ Sat, 27 Jun 2020 00:07:46: 35000000 INFO @ Sat, 27 Jun 2020 00:07:50: 31000000 INFO @ Sat, 27 Jun 2020 00:07:52: 23000000 INFO @ Sat, 27 Jun 2020 00:07:53: 36000000 INFO @ Sat, 27 Jun 2020 00:07:56: 32000000 INFO @ Sat, 27 Jun 2020 00:07:59: 37000000 INFO @ Sat, 27 Jun 2020 00:08:00: 24000000 INFO @ Sat, 27 Jun 2020 00:08:03: 33000000 INFO @ Sat, 27 Jun 2020 00:08:06: 38000000 INFO @ Sat, 27 Jun 2020 00:08:07: 25000000 INFO @ Sat, 27 Jun 2020 00:08:09: 34000000 INFO @ Sat, 27 Jun 2020 00:08:12: 39000000 INFO @ Sat, 27 Jun 2020 00:08:14: 26000000 INFO @ Sat, 27 Jun 2020 00:08:16: 35000000 INFO @ Sat, 27 Jun 2020 00:08:19: 40000000 INFO @ Sat, 27 Jun 2020 00:08:21: 27000000 INFO @ Sat, 27 Jun 2020 00:08:22: 36000000 INFO @ Sat, 27 Jun 2020 00:08:25: 41000000 INFO @ Sat, 27 Jun 2020 00:08:28: 28000000 INFO @ Sat, 27 Jun 2020 00:08:29: 37000000 INFO @ Sat, 27 Jun 2020 00:08:31: 42000000 INFO @ Sat, 27 Jun 2020 00:08:35: 38000000 INFO @ Sat, 27 Jun 2020 00:08:35: 29000000 INFO @ Sat, 27 Jun 2020 00:08:37: 43000000 INFO @ Sat, 27 Jun 2020 00:08:41: 39000000 INFO @ Sat, 27 Jun 2020 00:08:42: 30000000 INFO @ Sat, 27 Jun 2020 00:08:44: 44000000 INFO @ Sat, 27 Jun 2020 00:08:48: 40000000 INFO @ Sat, 27 Jun 2020 00:08:50: 31000000 INFO @ Sat, 27 Jun 2020 00:08:50: 45000000 INFO @ Sat, 27 Jun 2020 00:08:54: 41000000 INFO @ Sat, 27 Jun 2020 00:08:56: 46000000 INFO @ Sat, 27 Jun 2020 00:08:57: 32000000 INFO @ Sat, 27 Jun 2020 00:09:00: 42000000 INFO @ Sat, 27 Jun 2020 00:09:02: 47000000 INFO @ Sat, 27 Jun 2020 00:09:04: 33000000 INFO @ Sat, 27 Jun 2020 00:09:07: 43000000 INFO @ Sat, 27 Jun 2020 00:09:09: 48000000 INFO @ Sat, 27 Jun 2020 00:09:12: 34000000 INFO @ Sat, 27 Jun 2020 00:09:13: 44000000 INFO @ Sat, 27 Jun 2020 00:09:15: 49000000 INFO @ Sat, 27 Jun 2020 00:09:19: 45000000 INFO @ Sat, 27 Jun 2020 00:09:19: 35000000 INFO @ Sat, 27 Jun 2020 00:09:21: 50000000 INFO @ Sat, 27 Jun 2020 00:09:25: 46000000 INFO @ Sat, 27 Jun 2020 00:09:27: 36000000 INFO @ Sat, 27 Jun 2020 00:09:28: 51000000 INFO @ Sat, 27 Jun 2020 00:09:31: 47000000 INFO @ Sat, 27 Jun 2020 00:09:34: 52000000 INFO @ Sat, 27 Jun 2020 00:09:34: 37000000 INFO @ Sat, 27 Jun 2020 00:09:38: 48000000 INFO @ Sat, 27 Jun 2020 00:09:41: 53000000 INFO @ Sat, 27 Jun 2020 00:09:41: 38000000 INFO @ Sat, 27 Jun 2020 00:09:44: 49000000 INFO @ Sat, 27 Jun 2020 00:09:47: 54000000 INFO @ Sat, 27 Jun 2020 00:09:49: 39000000 INFO @ Sat, 27 Jun 2020 00:09:50: 50000000 INFO @ Sat, 27 Jun 2020 00:09:54: 55000000 INFO @ Sat, 27 Jun 2020 00:09:56: 40000000 INFO @ Sat, 27 Jun 2020 00:09:57: 51000000 INFO @ Sat, 27 Jun 2020 00:10:01: 56000000 INFO @ Sat, 27 Jun 2020 00:10:03: 41000000 INFO @ Sat, 27 Jun 2020 00:10:04: 52000000 INFO @ Sat, 27 Jun 2020 00:10:08: 57000000 INFO @ Sat, 27 Jun 2020 00:10:10: 42000000 INFO @ Sat, 27 Jun 2020 00:10:11: 53000000 INFO @ Sat, 27 Jun 2020 00:10:14: 58000000 INFO @ Sat, 27 Jun 2020 00:10:18: 43000000 INFO @ Sat, 27 Jun 2020 00:10:18: 54000000 INFO @ Sat, 27 Jun 2020 00:10:21: 59000000 INFO @ Sat, 27 Jun 2020 00:10:25: 55000000 INFO @ Sat, 27 Jun 2020 00:10:25: 44000000 INFO @ Sat, 27 Jun 2020 00:10:27: 60000000 INFO @ Sat, 27 Jun 2020 00:10:31: 56000000 INFO @ Sat, 27 Jun 2020 00:10:33: 45000000 INFO @ Sat, 27 Jun 2020 00:10:34: 61000000 INFO @ Sat, 27 Jun 2020 00:10:38: 57000000 INFO @ Sat, 27 Jun 2020 00:10:40: 62000000 INFO @ Sat, 27 Jun 2020 00:10:40: 46000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 27 Jun 2020 00:10:44: 58000000 INFO @ Sat, 27 Jun 2020 00:10:46: 63000000 INFO @ Sat, 27 Jun 2020 00:10:47: 47000000 INFO @ Sat, 27 Jun 2020 00:10:51: 59000000 INFO @ Sat, 27 Jun 2020 00:10:53: 64000000 INFO @ Sat, 27 Jun 2020 00:10:55: 48000000 INFO @ Sat, 27 Jun 2020 00:10:57: 60000000 INFO @ Sat, 27 Jun 2020 00:10:59: 65000000 INFO @ Sat, 27 Jun 2020 00:11:02: 49000000 INFO @ Sat, 27 Jun 2020 00:11:04: 61000000 INFO @ Sat, 27 Jun 2020 00:11:06: 66000000 INFO @ Sat, 27 Jun 2020 00:11:09: #1 tag size is determined as 101 bps INFO @ Sat, 27 Jun 2020 00:11:09: #1 tag size = 101 INFO @ Sat, 27 Jun 2020 00:11:09: #1 total tags in treatment: 32900306 INFO @ Sat, 27 Jun 2020 00:11:09: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:11:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:11:10: 50000000 INFO @ Sat, 27 Jun 2020 00:11:10: #1 tags after filtering in treatment: 28804865 INFO @ Sat, 27 Jun 2020 00:11:10: #1 Redundant rate of treatment: 0.12 INFO @ Sat, 27 Jun 2020 00:11:10: #1 finished! INFO @ Sat, 27 Jun 2020 00:11:10: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:11:10: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:11:10: 62000000 INFO @ Sat, 27 Jun 2020 00:11:12: #2 number of paired peaks: 123 WARNING @ Sat, 27 Jun 2020 00:11:12: Fewer paired peaks (123) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 123 pairs to build model! INFO @ Sat, 27 Jun 2020 00:11:12: start model_add_line... INFO @ Sat, 27 Jun 2020 00:11:12: start X-correlation... INFO @ Sat, 27 Jun 2020 00:11:12: end of X-cor INFO @ Sat, 27 Jun 2020 00:11:12: #2 finished! INFO @ Sat, 27 Jun 2020 00:11:12: #2 predicted fragment length is 106 bps INFO @ Sat, 27 Jun 2020 00:11:12: #2 alternative fragment length(s) may be 2,83,106,133,172,183,202 bps INFO @ Sat, 27 Jun 2020 00:11:12: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX1674103/SRX1674103.05_model.r WARNING @ Sat, 27 Jun 2020 00:11:12: #2 Since the d (106) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 27 Jun 2020 00:11:12: #2 You may need to consider one of the other alternative d(s): 2,83,106,133,172,183,202 WARNING @ Sat, 27 Jun 2020 00:11:12: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 27 Jun 2020 00:11:12: #3 Call peaks... INFO @ Sat, 27 Jun 2020 00:11:12: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 27 Jun 2020 00:11:17: 63000000 INFO @ Sat, 27 Jun 2020 00:11:18: 51000000 INFO @ Sat, 27 Jun 2020 00:11:23: 64000000 INFO @ Sat, 27 Jun 2020 00:11:26: 52000000 INFO @ Sat, 27 Jun 2020 00:11:29: 65000000 INFO @ Sat, 27 Jun 2020 00:11:34: 53000000 INFO @ Sat, 27 Jun 2020 00:11:35: 66000000 INFO @ Sat, 27 Jun 2020 00:11:39: #1 tag size is determined as 101 bps INFO @ Sat, 27 Jun 2020 00:11:39: #1 tag size = 101 INFO @ Sat, 27 Jun 2020 00:11:39: #1 total tags in treatment: 32900306 INFO @ Sat, 27 Jun 2020 00:11:39: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:11:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:11:39: #1 tags after filtering in treatment: 28804865 INFO @ Sat, 27 Jun 2020 00:11:39: #1 Redundant rate of treatment: 0.12 INFO @ Sat, 27 Jun 2020 00:11:39: #1 finished! INFO @ Sat, 27 Jun 2020 00:11:39: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:11:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:11:41: #2 number of paired peaks: 123 WARNING @ Sat, 27 Jun 2020 00:11:41: Fewer paired peaks (123) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 123 pairs to build model! INFO @ Sat, 27 Jun 2020 00:11:41: start model_add_line... INFO @ Sat, 27 Jun 2020 00:11:42: start X-correlation... INFO @ Sat, 27 Jun 2020 00:11:42: end of X-cor INFO @ Sat, 27 Jun 2020 00:11:42: #2 finished! INFO @ Sat, 27 Jun 2020 00:11:42: #2 predicted fragment length is 106 bps INFO @ Sat, 27 Jun 2020 00:11:42: #2 alternative fragment length(s) may be 2,83,106,133,172,183,202 bps INFO @ Sat, 27 Jun 2020 00:11:42: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX1674103/SRX1674103.10_model.r WARNING @ Sat, 27 Jun 2020 00:11:42: #2 Since the d (106) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 27 Jun 2020 00:11:42: #2 You may need to consider one of the other alternative d(s): 2,83,106,133,172,183,202 WARNING @ Sat, 27 Jun 2020 00:11:42: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 27 Jun 2020 00:11:42: #3 Call peaks... INFO @ Sat, 27 Jun 2020 00:11:42: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 27 Jun 2020 00:11:42: 54000000 INFO @ Sat, 27 Jun 2020 00:11:49: 55000000 INFO @ Sat, 27 Jun 2020 00:11:57: 56000000 INFO @ Sat, 27 Jun 2020 00:12:02: #3 Call peaks for each chromosome... INFO @ Sat, 27 Jun 2020 00:12:05: 57000000 INFO @ Sat, 27 Jun 2020 00:12:13: 58000000 BigWig に変換しました。 INFO @ Sat, 27 Jun 2020 00:12:20: 59000000 INFO @ Sat, 27 Jun 2020 00:12:25: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX1674103/SRX1674103.05_peaks.xls INFO @ Sat, 27 Jun 2020 00:12:25: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX1674103/SRX1674103.05_peaks.narrowPeak INFO @ Sat, 27 Jun 2020 00:12:25: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX1674103/SRX1674103.05_summits.bed INFO @ Sat, 27 Jun 2020 00:12:25: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (596 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Sat, 27 Jun 2020 00:12:27: 60000000 INFO @ Sat, 27 Jun 2020 00:12:31: #3 Call peaks for each chromosome... INFO @ Sat, 27 Jun 2020 00:12:34: 61000000 INFO @ Sat, 27 Jun 2020 00:12:41: 62000000 INFO @ Sat, 27 Jun 2020 00:12:48: 63000000 INFO @ Sat, 27 Jun 2020 00:12:54: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX1674103/SRX1674103.10_peaks.xls INFO @ Sat, 27 Jun 2020 00:12:54: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX1674103/SRX1674103.10_peaks.narrowPeak INFO @ Sat, 27 Jun 2020 00:12:54: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX1674103/SRX1674103.10_summits.bed INFO @ Sat, 27 Jun 2020 00:12:54: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (423 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Sat, 27 Jun 2020 00:12:55: 64000000 INFO @ Sat, 27 Jun 2020 00:13:02: 65000000 INFO @ Sat, 27 Jun 2020 00:13:09: 66000000 INFO @ Sat, 27 Jun 2020 00:13:13: #1 tag size is determined as 101 bps INFO @ Sat, 27 Jun 2020 00:13:13: #1 tag size = 101 INFO @ Sat, 27 Jun 2020 00:13:13: #1 total tags in treatment: 32900306 INFO @ Sat, 27 Jun 2020 00:13:13: #1 user defined the maximum tags... INFO @ Sat, 27 Jun 2020 00:13:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 27 Jun 2020 00:13:14: #1 tags after filtering in treatment: 28804865 INFO @ Sat, 27 Jun 2020 00:13:14: #1 Redundant rate of treatment: 0.12 INFO @ Sat, 27 Jun 2020 00:13:14: #1 finished! INFO @ Sat, 27 Jun 2020 00:13:14: #2 Build Peak Model... INFO @ Sat, 27 Jun 2020 00:13:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 27 Jun 2020 00:13:16: #2 number of paired peaks: 123 WARNING @ Sat, 27 Jun 2020 00:13:16: Fewer paired peaks (123) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 123 pairs to build model! INFO @ Sat, 27 Jun 2020 00:13:16: start model_add_line... INFO @ Sat, 27 Jun 2020 00:13:16: start X-correlation... INFO @ Sat, 27 Jun 2020 00:13:16: end of X-cor INFO @ Sat, 27 Jun 2020 00:13:16: #2 finished! INFO @ Sat, 27 Jun 2020 00:13:16: #2 predicted fragment length is 106 bps INFO @ Sat, 27 Jun 2020 00:13:16: #2 alternative fragment length(s) may be 2,83,106,133,172,183,202 bps INFO @ Sat, 27 Jun 2020 00:13:16: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX1674103/SRX1674103.20_model.r WARNING @ Sat, 27 Jun 2020 00:13:16: #2 Since the d (106) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 27 Jun 2020 00:13:16: #2 You may need to consider one of the other alternative d(s): 2,83,106,133,172,183,202 WARNING @ Sat, 27 Jun 2020 00:13:16: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 27 Jun 2020 00:13:16: #3 Call peaks... INFO @ Sat, 27 Jun 2020 00:13:16: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 27 Jun 2020 00:14:06: #3 Call peaks for each chromosome... INFO @ Sat, 27 Jun 2020 00:14:29: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX1674103/SRX1674103.20_peaks.xls INFO @ Sat, 27 Jun 2020 00:14:29: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX1674103/SRX1674103.20_peaks.narrowPeak INFO @ Sat, 27 Jun 2020 00:14:29: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX1674103/SRX1674103.20_summits.bed INFO @ Sat, 27 Jun 2020 00:14:29: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (244 records, 4 fields): 2 millis CompletedMACS2peakCalling