Job ID = 6366513 SRX = SRX1674093 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T22:59:23 prefetch.2.10.7: 1) Downloading 'SRR3320139'... 2020-06-15T22:59:23 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:05:45 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:05:45 prefetch.2.10.7: 1) 'SRR3320139' was downloaded successfully Read 103005862 spots for SRR3320139/SRR3320139.sra Written 103005862 spots for SRR3320139/SRR3320139.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:22:35 103005862 reads; of these: 103005862 (100.00%) were unpaired; of these: 1293523 (1.26%) aligned 0 times 91196085 (88.53%) aligned exactly 1 time 10516254 (10.21%) aligned >1 times 98.74% overall alignment rate Time searching: 00:22:35 Overall time: 00:22:35 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 44 files... [bam_rmdupse_core] 59193076 / 101712339 = 0.5820 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:53:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1674093/SRX1674093.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1674093/SRX1674093.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1674093/SRX1674093.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1674093/SRX1674093.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:53:21: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:53:21: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:53:27: 1000000 INFO @ Tue, 16 Jun 2020 08:53:32: 2000000 INFO @ Tue, 16 Jun 2020 08:53:37: 3000000 INFO @ Tue, 16 Jun 2020 08:53:43: 4000000 INFO @ Tue, 16 Jun 2020 08:53:48: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:53:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1674093/SRX1674093.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1674093/SRX1674093.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1674093/SRX1674093.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1674093/SRX1674093.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:53:51: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:53:51: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:53:54: 6000000 INFO @ Tue, 16 Jun 2020 08:53:57: 1000000 INFO @ Tue, 16 Jun 2020 08:53:59: 7000000 INFO @ Tue, 16 Jun 2020 08:54:02: 2000000 INFO @ Tue, 16 Jun 2020 08:54:05: 8000000 INFO @ Tue, 16 Jun 2020 08:54:08: 3000000 INFO @ Tue, 16 Jun 2020 08:54:11: 9000000 INFO @ Tue, 16 Jun 2020 08:54:13: 4000000 INFO @ Tue, 16 Jun 2020 08:54:17: 10000000 INFO @ Tue, 16 Jun 2020 08:54:19: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:54:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1674093/SRX1674093.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1674093/SRX1674093.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1674093/SRX1674093.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1674093/SRX1674093.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:54:21: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:54:21: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:54:22: 11000000 INFO @ Tue, 16 Jun 2020 08:54:24: 6000000 INFO @ Tue, 16 Jun 2020 08:54:27: 1000000 INFO @ Tue, 16 Jun 2020 08:54:28: 12000000 INFO @ Tue, 16 Jun 2020 08:54:30: 7000000 INFO @ Tue, 16 Jun 2020 08:54:32: 2000000 INFO @ Tue, 16 Jun 2020 08:54:34: 13000000 INFO @ Tue, 16 Jun 2020 08:54:36: 8000000 INFO @ Tue, 16 Jun 2020 08:54:38: 3000000 INFO @ Tue, 16 Jun 2020 08:54:39: 14000000 INFO @ Tue, 16 Jun 2020 08:54:41: 9000000 INFO @ Tue, 16 Jun 2020 08:54:43: 4000000 INFO @ Tue, 16 Jun 2020 08:54:45: 15000000 INFO @ Tue, 16 Jun 2020 08:54:47: 10000000 INFO @ Tue, 16 Jun 2020 08:54:49: 5000000 INFO @ Tue, 16 Jun 2020 08:54:51: 16000000 INFO @ Tue, 16 Jun 2020 08:54:53: 11000000 INFO @ Tue, 16 Jun 2020 08:54:55: 6000000 INFO @ Tue, 16 Jun 2020 08:54:57: 17000000 INFO @ Tue, 16 Jun 2020 08:54:58: 12000000 INFO @ Tue, 16 Jun 2020 08:55:00: 7000000 INFO @ Tue, 16 Jun 2020 08:55:02: 18000000 INFO @ Tue, 16 Jun 2020 08:55:04: 13000000 INFO @ Tue, 16 Jun 2020 08:55:06: 8000000 INFO @ Tue, 16 Jun 2020 08:55:08: 19000000 INFO @ Tue, 16 Jun 2020 08:55:09: 14000000 INFO @ Tue, 16 Jun 2020 08:55:12: 9000000 INFO @ Tue, 16 Jun 2020 08:55:14: 20000000 INFO @ Tue, 16 Jun 2020 08:55:15: 15000000 INFO @ Tue, 16 Jun 2020 08:55:17: 10000000 INFO @ Tue, 16 Jun 2020 08:55:20: 21000000 INFO @ Tue, 16 Jun 2020 08:55:21: 16000000 INFO @ Tue, 16 Jun 2020 08:55:23: 11000000 INFO @ Tue, 16 Jun 2020 08:55:25: 22000000 INFO @ Tue, 16 Jun 2020 08:55:27: 17000000 INFO @ Tue, 16 Jun 2020 08:55:29: 12000000 INFO @ Tue, 16 Jun 2020 08:55:31: 23000000 INFO @ Tue, 16 Jun 2020 08:55:33: 18000000 INFO @ Tue, 16 Jun 2020 08:55:35: 13000000 INFO @ Tue, 16 Jun 2020 08:55:37: 24000000 INFO @ Tue, 16 Jun 2020 08:55:38: 19000000 INFO @ Tue, 16 Jun 2020 08:55:40: 14000000 INFO @ Tue, 16 Jun 2020 08:55:43: 25000000 INFO @ Tue, 16 Jun 2020 08:55:44: 20000000 INFO @ Tue, 16 Jun 2020 08:55:46: 15000000 INFO @ Tue, 16 Jun 2020 08:55:49: 26000000 INFO @ Tue, 16 Jun 2020 08:55:50: 21000000 INFO @ Tue, 16 Jun 2020 08:55:52: 16000000 INFO @ Tue, 16 Jun 2020 08:55:55: 27000000 INFO @ Tue, 16 Jun 2020 08:55:55: 22000000 INFO @ Tue, 16 Jun 2020 08:55:58: 17000000 INFO @ Tue, 16 Jun 2020 08:56:01: 28000000 INFO @ Tue, 16 Jun 2020 08:56:01: 23000000 INFO @ Tue, 16 Jun 2020 08:56:04: 18000000 INFO @ Tue, 16 Jun 2020 08:56:06: 29000000 INFO @ Tue, 16 Jun 2020 08:56:07: 24000000 INFO @ Tue, 16 Jun 2020 08:56:10: 19000000 INFO @ Tue, 16 Jun 2020 08:56:12: 30000000 INFO @ Tue, 16 Jun 2020 08:56:13: 25000000 INFO @ Tue, 16 Jun 2020 08:56:15: 20000000 INFO @ Tue, 16 Jun 2020 08:56:18: 31000000 INFO @ Tue, 16 Jun 2020 08:56:18: 26000000 INFO @ Tue, 16 Jun 2020 08:56:21: 21000000 INFO @ Tue, 16 Jun 2020 08:56:24: 32000000 INFO @ Tue, 16 Jun 2020 08:56:24: 27000000 INFO @ Tue, 16 Jun 2020 08:56:27: 22000000 INFO @ Tue, 16 Jun 2020 08:56:30: 28000000 INFO @ Tue, 16 Jun 2020 08:56:30: 33000000 INFO @ Tue, 16 Jun 2020 08:56:33: 23000000 INFO @ Tue, 16 Jun 2020 08:56:35: 29000000 INFO @ Tue, 16 Jun 2020 08:56:36: 34000000 INFO @ Tue, 16 Jun 2020 08:56:39: 24000000 INFO @ Tue, 16 Jun 2020 08:56:41: 30000000 INFO @ Tue, 16 Jun 2020 08:56:42: 35000000 INFO @ Tue, 16 Jun 2020 08:56:45: 25000000 INFO @ Tue, 16 Jun 2020 08:56:47: 31000000 INFO @ Tue, 16 Jun 2020 08:56:47: 36000000 INFO @ Tue, 16 Jun 2020 08:56:50: 26000000 INFO @ Tue, 16 Jun 2020 08:56:53: 32000000 INFO @ Tue, 16 Jun 2020 08:56:53: 37000000 INFO @ Tue, 16 Jun 2020 08:56:56: 27000000 INFO @ Tue, 16 Jun 2020 08:56:59: 33000000 INFO @ Tue, 16 Jun 2020 08:56:59: 38000000 INFO @ Tue, 16 Jun 2020 08:57:02: 28000000 INFO @ Tue, 16 Jun 2020 08:57:04: 34000000 INFO @ Tue, 16 Jun 2020 08:57:05: 39000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 08:57:08: 29000000 INFO @ Tue, 16 Jun 2020 08:57:10: 35000000 INFO @ Tue, 16 Jun 2020 08:57:11: 40000000 INFO @ Tue, 16 Jun 2020 08:57:13: 30000000 INFO @ Tue, 16 Jun 2020 08:57:16: 36000000 INFO @ Tue, 16 Jun 2020 08:57:17: 41000000 INFO @ Tue, 16 Jun 2020 08:57:19: 31000000 INFO @ Tue, 16 Jun 2020 08:57:21: 37000000 INFO @ Tue, 16 Jun 2020 08:57:22: 42000000 INFO @ Tue, 16 Jun 2020 08:57:25: 32000000 INFO @ Tue, 16 Jun 2020 08:57:26: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 08:57:26: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 08:57:26: #1 total tags in treatment: 42519263 INFO @ Tue, 16 Jun 2020 08:57:26: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:57:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:57:27: #1 tags after filtering in treatment: 42519263 INFO @ Tue, 16 Jun 2020 08:57:27: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:57:27: #1 finished! INFO @ Tue, 16 Jun 2020 08:57:27: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:57:27: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:57:27: 38000000 INFO @ Tue, 16 Jun 2020 08:57:29: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 08:57:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:57:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX1674093/SRX1674093.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674093/SRX1674093.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674093/SRX1674093.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674093/SRX1674093.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:57:30: 33000000 INFO @ Tue, 16 Jun 2020 08:57:33: 39000000 INFO @ Tue, 16 Jun 2020 08:57:36: 34000000 INFO @ Tue, 16 Jun 2020 08:57:38: 40000000 INFO @ Tue, 16 Jun 2020 08:57:42: 35000000 INFO @ Tue, 16 Jun 2020 08:57:44: 41000000 INFO @ Tue, 16 Jun 2020 08:57:47: 36000000 INFO @ Tue, 16 Jun 2020 08:57:50: 42000000 INFO @ Tue, 16 Jun 2020 08:57:53: 37000000 INFO @ Tue, 16 Jun 2020 08:57:53: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 08:57:53: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 08:57:53: #1 total tags in treatment: 42519263 INFO @ Tue, 16 Jun 2020 08:57:53: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:57:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:57:54: #1 tags after filtering in treatment: 42519263 INFO @ Tue, 16 Jun 2020 08:57:54: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:57:54: #1 finished! INFO @ Tue, 16 Jun 2020 08:57:54: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:57:54: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:57:56: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 08:57:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:57:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX1674093/SRX1674093.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674093/SRX1674093.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674093/SRX1674093.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674093/SRX1674093.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:57:58: 38000000 INFO @ Tue, 16 Jun 2020 08:58:04: 39000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 08:58:09: 40000000 INFO @ Tue, 16 Jun 2020 08:58:14: 41000000 INFO @ Tue, 16 Jun 2020 08:58:20: 42000000 INFO @ Tue, 16 Jun 2020 08:58:23: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 08:58:23: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 08:58:23: #1 total tags in treatment: 42519263 INFO @ Tue, 16 Jun 2020 08:58:23: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:58:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:58:23: #1 tags after filtering in treatment: 42519263 INFO @ Tue, 16 Jun 2020 08:58:23: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:58:23: #1 finished! INFO @ Tue, 16 Jun 2020 08:58:23: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:58:23: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:58:26: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 08:58:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:58:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX1674093/SRX1674093.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674093/SRX1674093.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674093/SRX1674093.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674093/SRX1674093.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling