Job ID = 6366508 SRX = SRX1674088 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T22:56:14 prefetch.2.10.7: 1) Downloading 'SRR3320134'... 2020-06-15T22:56:14 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:06:06 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:06:06 prefetch.2.10.7: 1) 'SRR3320134' was downloaded successfully Read 98384435 spots for SRR3320134/SRR3320134.sra Written 98384435 spots for SRR3320134/SRR3320134.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:43:54 98384435 reads; of these: 98384435 (100.00%) were unpaired; of these: 11006891 (11.19%) aligned 0 times 73674831 (74.88%) aligned exactly 1 time 13702713 (13.93%) aligned >1 times 88.81% overall alignment rate Time searching: 00:43:54 Overall time: 00:43:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 36 files... [bam_rmdupse_core] 42225900 / 87377544 = 0.4833 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:21:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1674088/SRX1674088.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1674088/SRX1674088.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1674088/SRX1674088.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1674088/SRX1674088.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:21:34: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:21:34: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:21:43: 1000000 INFO @ Tue, 16 Jun 2020 09:21:52: 2000000 INFO @ Tue, 16 Jun 2020 09:22:00: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:22:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1674088/SRX1674088.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1674088/SRX1674088.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1674088/SRX1674088.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1674088/SRX1674088.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:22:03: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:22:03: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:22:10: 4000000 INFO @ Tue, 16 Jun 2020 09:22:13: 1000000 INFO @ Tue, 16 Jun 2020 09:22:19: 5000000 INFO @ Tue, 16 Jun 2020 09:22:22: 2000000 INFO @ Tue, 16 Jun 2020 09:22:29: 6000000 INFO @ Tue, 16 Jun 2020 09:22:31: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:22:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1674088/SRX1674088.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1674088/SRX1674088.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1674088/SRX1674088.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1674088/SRX1674088.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:22:34: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:22:34: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:22:39: 7000000 INFO @ Tue, 16 Jun 2020 09:22:40: 4000000 INFO @ Tue, 16 Jun 2020 09:22:43: 1000000 INFO @ Tue, 16 Jun 2020 09:22:49: 8000000 INFO @ Tue, 16 Jun 2020 09:22:50: 5000000 INFO @ Tue, 16 Jun 2020 09:22:52: 2000000 INFO @ Tue, 16 Jun 2020 09:22:58: 9000000 INFO @ Tue, 16 Jun 2020 09:22:59: 6000000 INFO @ Tue, 16 Jun 2020 09:23:02: 3000000 INFO @ Tue, 16 Jun 2020 09:23:08: 7000000 INFO @ Tue, 16 Jun 2020 09:23:09: 10000000 INFO @ Tue, 16 Jun 2020 09:23:11: 4000000 INFO @ Tue, 16 Jun 2020 09:23:18: 8000000 INFO @ Tue, 16 Jun 2020 09:23:19: 11000000 INFO @ Tue, 16 Jun 2020 09:23:20: 5000000 INFO @ Tue, 16 Jun 2020 09:23:27: 9000000 INFO @ Tue, 16 Jun 2020 09:23:29: 12000000 INFO @ Tue, 16 Jun 2020 09:23:30: 6000000 INFO @ Tue, 16 Jun 2020 09:23:36: 10000000 INFO @ Tue, 16 Jun 2020 09:23:39: 13000000 INFO @ Tue, 16 Jun 2020 09:23:39: 7000000 INFO @ Tue, 16 Jun 2020 09:23:46: 11000000 INFO @ Tue, 16 Jun 2020 09:23:48: 8000000 INFO @ Tue, 16 Jun 2020 09:23:49: 14000000 INFO @ Tue, 16 Jun 2020 09:23:55: 12000000 INFO @ Tue, 16 Jun 2020 09:23:58: 9000000 INFO @ Tue, 16 Jun 2020 09:23:59: 15000000 INFO @ Tue, 16 Jun 2020 09:24:04: 13000000 INFO @ Tue, 16 Jun 2020 09:24:07: 10000000 INFO @ Tue, 16 Jun 2020 09:24:09: 16000000 INFO @ Tue, 16 Jun 2020 09:24:14: 14000000 INFO @ Tue, 16 Jun 2020 09:24:16: 11000000 INFO @ Tue, 16 Jun 2020 09:24:19: 17000000 INFO @ Tue, 16 Jun 2020 09:24:23: 15000000 INFO @ Tue, 16 Jun 2020 09:24:25: 12000000 INFO @ Tue, 16 Jun 2020 09:24:29: 18000000 INFO @ Tue, 16 Jun 2020 09:24:32: 16000000 INFO @ Tue, 16 Jun 2020 09:24:35: 13000000 INFO @ Tue, 16 Jun 2020 09:24:40: 19000000 INFO @ Tue, 16 Jun 2020 09:24:42: 17000000 INFO @ Tue, 16 Jun 2020 09:24:44: 14000000 INFO @ Tue, 16 Jun 2020 09:24:50: 20000000 INFO @ Tue, 16 Jun 2020 09:24:51: 18000000 INFO @ Tue, 16 Jun 2020 09:24:53: 15000000 INFO @ Tue, 16 Jun 2020 09:25:00: 19000000 INFO @ Tue, 16 Jun 2020 09:25:01: 21000000 INFO @ Tue, 16 Jun 2020 09:25:03: 16000000 INFO @ Tue, 16 Jun 2020 09:25:09: 20000000 INFO @ Tue, 16 Jun 2020 09:25:11: 22000000 INFO @ Tue, 16 Jun 2020 09:25:12: 17000000 INFO @ Tue, 16 Jun 2020 09:25:19: 21000000 INFO @ Tue, 16 Jun 2020 09:25:21: 18000000 INFO @ Tue, 16 Jun 2020 09:25:21: 23000000 INFO @ Tue, 16 Jun 2020 09:25:28: 22000000 INFO @ Tue, 16 Jun 2020 09:25:30: 19000000 INFO @ Tue, 16 Jun 2020 09:25:32: 24000000 INFO @ Tue, 16 Jun 2020 09:25:37: 23000000 INFO @ Tue, 16 Jun 2020 09:25:40: 20000000 INFO @ Tue, 16 Jun 2020 09:25:42: 25000000 INFO @ Tue, 16 Jun 2020 09:25:46: 24000000 INFO @ Tue, 16 Jun 2020 09:25:49: 21000000 INFO @ Tue, 16 Jun 2020 09:25:52: 26000000 INFO @ Tue, 16 Jun 2020 09:25:56: 25000000 INFO @ Tue, 16 Jun 2020 09:25:58: 22000000 INFO @ Tue, 16 Jun 2020 09:26:02: 27000000 INFO @ Tue, 16 Jun 2020 09:26:05: 26000000 INFO @ Tue, 16 Jun 2020 09:26:07: 23000000 INFO @ Tue, 16 Jun 2020 09:26:12: 28000000 INFO @ Tue, 16 Jun 2020 09:26:14: 27000000 INFO @ Tue, 16 Jun 2020 09:26:17: 24000000 INFO @ Tue, 16 Jun 2020 09:26:22: 29000000 INFO @ Tue, 16 Jun 2020 09:26:24: 28000000 INFO @ Tue, 16 Jun 2020 09:26:26: 25000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:26:33: 30000000 INFO @ Tue, 16 Jun 2020 09:26:33: 29000000 INFO @ Tue, 16 Jun 2020 09:26:35: 26000000 INFO @ Tue, 16 Jun 2020 09:26:42: 30000000 INFO @ Tue, 16 Jun 2020 09:26:43: 31000000 INFO @ Tue, 16 Jun 2020 09:26:44: 27000000 INFO @ Tue, 16 Jun 2020 09:26:52: 31000000 INFO @ Tue, 16 Jun 2020 09:26:53: 32000000 INFO @ Tue, 16 Jun 2020 09:26:54: 28000000 INFO @ Tue, 16 Jun 2020 09:27:01: 32000000 INFO @ Tue, 16 Jun 2020 09:27:03: 29000000 INFO @ Tue, 16 Jun 2020 09:27:03: 33000000 INFO @ Tue, 16 Jun 2020 09:27:10: 33000000 INFO @ Tue, 16 Jun 2020 09:27:13: 30000000 INFO @ Tue, 16 Jun 2020 09:27:13: 34000000 INFO @ Tue, 16 Jun 2020 09:27:20: 34000000 INFO @ Tue, 16 Jun 2020 09:27:22: 31000000 INFO @ Tue, 16 Jun 2020 09:27:23: 35000000 INFO @ Tue, 16 Jun 2020 09:27:29: 35000000 INFO @ Tue, 16 Jun 2020 09:27:32: 32000000 INFO @ Tue, 16 Jun 2020 09:27:34: 36000000 INFO @ Tue, 16 Jun 2020 09:27:39: 36000000 INFO @ Tue, 16 Jun 2020 09:27:41: 33000000 INFO @ Tue, 16 Jun 2020 09:27:44: 37000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 09:27:48: 37000000 INFO @ Tue, 16 Jun 2020 09:27:50: 34000000 INFO @ Tue, 16 Jun 2020 09:27:54: 38000000 INFO @ Tue, 16 Jun 2020 09:27:57: 38000000 INFO @ Tue, 16 Jun 2020 09:28:00: 35000000 INFO @ Tue, 16 Jun 2020 09:28:04: 39000000 INFO @ Tue, 16 Jun 2020 09:28:07: 39000000 INFO @ Tue, 16 Jun 2020 09:28:09: 36000000 INFO @ Tue, 16 Jun 2020 09:28:14: 40000000 INFO @ Tue, 16 Jun 2020 09:28:16: 40000000 INFO @ Tue, 16 Jun 2020 09:28:19: 37000000 INFO @ Tue, 16 Jun 2020 09:28:25: 41000000 INFO @ Tue, 16 Jun 2020 09:28:26: 41000000 INFO @ Tue, 16 Jun 2020 09:28:28: 38000000 INFO @ Tue, 16 Jun 2020 09:28:35: 42000000 INFO @ Tue, 16 Jun 2020 09:28:36: 42000000 INFO @ Tue, 16 Jun 2020 09:28:38: 39000000 INFO @ Tue, 16 Jun 2020 09:28:46: 43000000 INFO @ Tue, 16 Jun 2020 09:28:47: 43000000 INFO @ Tue, 16 Jun 2020 09:28:47: 40000000 INFO @ Tue, 16 Jun 2020 09:28:56: 41000000 INFO @ Tue, 16 Jun 2020 09:28:58: 44000000 INFO @ Tue, 16 Jun 2020 09:28:58: 44000000 INFO @ Tue, 16 Jun 2020 09:29:06: 42000000 INFO @ Tue, 16 Jun 2020 09:29:08: 45000000 INFO @ Tue, 16 Jun 2020 09:29:09: 45000000 INFO @ Tue, 16 Jun 2020 09:29:09: #1 tag size is determined as 101 bps INFO @ Tue, 16 Jun 2020 09:29:09: #1 tag size = 101 INFO @ Tue, 16 Jun 2020 09:29:09: #1 total tags in treatment: 45151644 INFO @ Tue, 16 Jun 2020 09:29:09: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:29:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:29:10: #1 tags after filtering in treatment: 45151644 INFO @ Tue, 16 Jun 2020 09:29:10: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:29:10: #1 finished! INFO @ Tue, 16 Jun 2020 09:29:10: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:29:10: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:29:11: #1 tag size is determined as 101 bps INFO @ Tue, 16 Jun 2020 09:29:11: #1 tag size = 101 INFO @ Tue, 16 Jun 2020 09:29:11: #1 total tags in treatment: 45151644 INFO @ Tue, 16 Jun 2020 09:29:11: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:29:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:29:11: #1 tags after filtering in treatment: 45151644 INFO @ Tue, 16 Jun 2020 09:29:11: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:29:11: #1 finished! INFO @ Tue, 16 Jun 2020 09:29:11: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:29:11: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:29:13: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:29:13: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:29:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX1674088/SRX1674088.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674088/SRX1674088.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674088/SRX1674088.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674088/SRX1674088.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:29:14: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:29:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:29:14: Process for pairing-model is terminated! INFO @ Tue, 16 Jun 2020 09:29:14: 43000000 cut: /home/okishinya/chipatlas/results/ce11/SRX1674088/SRX1674088.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674088/SRX1674088.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674088/SRX1674088.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674088/SRX1674088.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:29:22: 44000000 INFO @ Tue, 16 Jun 2020 09:29:30: 45000000 INFO @ Tue, 16 Jun 2020 09:29:31: #1 tag size is determined as 101 bps INFO @ Tue, 16 Jun 2020 09:29:31: #1 tag size = 101 INFO @ Tue, 16 Jun 2020 09:29:31: #1 total tags in treatment: 45151644 INFO @ Tue, 16 Jun 2020 09:29:31: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:29:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:29:32: #1 tags after filtering in treatment: 45151644 INFO @ Tue, 16 Jun 2020 09:29:32: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:29:32: #1 finished! INFO @ Tue, 16 Jun 2020 09:29:32: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:29:32: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:29:35: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:29:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:29:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX1674088/SRX1674088.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674088/SRX1674088.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674088/SRX1674088.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674088/SRX1674088.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling