Job ID = 6366507 SRX = SRX1674087 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T22:47:52 prefetch.2.10.7: 1) Downloading 'SRR3320133'... 2020-06-15T22:47:52 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T22:51:41 prefetch.2.10.7: HTTPS download succeed 2020-06-15T22:51:41 prefetch.2.10.7: 1) 'SRR3320133' was downloaded successfully Read 66061416 spots for SRR3320133/SRR3320133.sra Written 66061416 spots for SRR3320133/SRR3320133.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:49 66061416 reads; of these: 66061416 (100.00%) were unpaired; of these: 13423906 (20.32%) aligned 0 times 45564459 (68.97%) aligned exactly 1 time 7073051 (10.71%) aligned >1 times 79.68% overall alignment rate Time searching: 00:13:49 Overall time: 00:13:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 18032936 / 52637510 = 0.3426 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:19:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1674087/SRX1674087.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1674087/SRX1674087.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1674087/SRX1674087.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1674087/SRX1674087.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:19:12: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:19:12: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:19:19: 1000000 INFO @ Tue, 16 Jun 2020 08:19:25: 2000000 INFO @ Tue, 16 Jun 2020 08:19:31: 3000000 INFO @ Tue, 16 Jun 2020 08:19:38: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:19:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1674087/SRX1674087.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1674087/SRX1674087.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1674087/SRX1674087.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1674087/SRX1674087.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:19:42: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:19:42: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:19:44: 5000000 INFO @ Tue, 16 Jun 2020 08:19:49: 1000000 INFO @ Tue, 16 Jun 2020 08:19:52: 6000000 INFO @ Tue, 16 Jun 2020 08:19:57: 2000000 INFO @ Tue, 16 Jun 2020 08:19:59: 7000000 INFO @ Tue, 16 Jun 2020 08:20:04: 3000000 INFO @ Tue, 16 Jun 2020 08:20:06: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:20:11: 4000000 INFO @ Tue, 16 Jun 2020 08:20:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1674087/SRX1674087.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1674087/SRX1674087.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1674087/SRX1674087.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1674087/SRX1674087.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:20:12: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:20:12: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:20:13: 9000000 INFO @ Tue, 16 Jun 2020 08:20:18: 5000000 INFO @ Tue, 16 Jun 2020 08:20:20: 1000000 INFO @ Tue, 16 Jun 2020 08:20:20: 10000000 INFO @ Tue, 16 Jun 2020 08:20:26: 6000000 INFO @ Tue, 16 Jun 2020 08:20:27: 2000000 INFO @ Tue, 16 Jun 2020 08:20:28: 11000000 INFO @ Tue, 16 Jun 2020 08:20:33: 7000000 INFO @ Tue, 16 Jun 2020 08:20:34: 3000000 INFO @ Tue, 16 Jun 2020 08:20:35: 12000000 INFO @ Tue, 16 Jun 2020 08:20:40: 8000000 INFO @ Tue, 16 Jun 2020 08:20:42: 4000000 INFO @ Tue, 16 Jun 2020 08:20:42: 13000000 INFO @ Tue, 16 Jun 2020 08:20:48: 9000000 INFO @ Tue, 16 Jun 2020 08:20:49: 5000000 INFO @ Tue, 16 Jun 2020 08:20:50: 14000000 INFO @ Tue, 16 Jun 2020 08:20:55: 10000000 INFO @ Tue, 16 Jun 2020 08:20:56: 6000000 INFO @ Tue, 16 Jun 2020 08:20:57: 15000000 INFO @ Tue, 16 Jun 2020 08:21:02: 11000000 INFO @ Tue, 16 Jun 2020 08:21:04: 7000000 INFO @ Tue, 16 Jun 2020 08:21:04: 16000000 INFO @ Tue, 16 Jun 2020 08:21:10: 12000000 INFO @ Tue, 16 Jun 2020 08:21:11: 8000000 INFO @ Tue, 16 Jun 2020 08:21:11: 17000000 INFO @ Tue, 16 Jun 2020 08:21:17: 13000000 INFO @ Tue, 16 Jun 2020 08:21:18: 9000000 INFO @ Tue, 16 Jun 2020 08:21:19: 18000000 INFO @ Tue, 16 Jun 2020 08:21:24: 14000000 INFO @ Tue, 16 Jun 2020 08:21:25: 10000000 INFO @ Tue, 16 Jun 2020 08:21:26: 19000000 INFO @ Tue, 16 Jun 2020 08:21:31: 15000000 INFO @ Tue, 16 Jun 2020 08:21:32: 11000000 INFO @ Tue, 16 Jun 2020 08:21:33: 20000000 INFO @ Tue, 16 Jun 2020 08:21:39: 16000000 INFO @ Tue, 16 Jun 2020 08:21:40: 12000000 INFO @ Tue, 16 Jun 2020 08:21:41: 21000000 INFO @ Tue, 16 Jun 2020 08:21:46: 17000000 INFO @ Tue, 16 Jun 2020 08:21:47: 13000000 INFO @ Tue, 16 Jun 2020 08:21:48: 22000000 INFO @ Tue, 16 Jun 2020 08:21:53: 18000000 INFO @ Tue, 16 Jun 2020 08:21:54: 14000000 INFO @ Tue, 16 Jun 2020 08:21:55: 23000000 INFO @ Tue, 16 Jun 2020 08:22:01: 19000000 INFO @ Tue, 16 Jun 2020 08:22:01: 15000000 INFO @ Tue, 16 Jun 2020 08:22:02: 24000000 INFO @ Tue, 16 Jun 2020 08:22:08: 20000000 INFO @ Tue, 16 Jun 2020 08:22:08: 16000000 INFO @ Tue, 16 Jun 2020 08:22:09: 25000000 INFO @ Tue, 16 Jun 2020 08:22:15: 17000000 INFO @ Tue, 16 Jun 2020 08:22:15: 21000000 INFO @ Tue, 16 Jun 2020 08:22:16: 26000000 INFO @ Tue, 16 Jun 2020 08:22:22: 18000000 INFO @ Tue, 16 Jun 2020 08:22:23: 22000000 INFO @ Tue, 16 Jun 2020 08:22:23: 27000000 INFO @ Tue, 16 Jun 2020 08:22:30: 19000000 INFO @ Tue, 16 Jun 2020 08:22:30: 23000000 INFO @ Tue, 16 Jun 2020 08:22:30: 28000000 INFO @ Tue, 16 Jun 2020 08:22:37: 20000000 INFO @ Tue, 16 Jun 2020 08:22:38: 24000000 INFO @ Tue, 16 Jun 2020 08:22:38: 29000000 INFO @ Tue, 16 Jun 2020 08:22:44: 21000000 INFO @ Tue, 16 Jun 2020 08:22:45: 25000000 INFO @ Tue, 16 Jun 2020 08:22:46: 30000000 INFO @ Tue, 16 Jun 2020 08:22:52: 22000000 INFO @ Tue, 16 Jun 2020 08:22:53: 26000000 INFO @ Tue, 16 Jun 2020 08:22:53: 31000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 08:22:59: 23000000 INFO @ Tue, 16 Jun 2020 08:23:00: 27000000 INFO @ Tue, 16 Jun 2020 08:23:00: 32000000 INFO @ Tue, 16 Jun 2020 08:23:07: 24000000 INFO @ Tue, 16 Jun 2020 08:23:07: 28000000 INFO @ Tue, 16 Jun 2020 08:23:08: 33000000 INFO @ Tue, 16 Jun 2020 08:23:14: 25000000 INFO @ Tue, 16 Jun 2020 08:23:15: 29000000 INFO @ Tue, 16 Jun 2020 08:23:15: 34000000 INFO @ Tue, 16 Jun 2020 08:23:19: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 08:23:19: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 08:23:19: #1 total tags in treatment: 34604574 INFO @ Tue, 16 Jun 2020 08:23:19: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:23:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:23:20: #1 tags after filtering in treatment: 34604574 INFO @ Tue, 16 Jun 2020 08:23:20: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:23:20: #1 finished! INFO @ Tue, 16 Jun 2020 08:23:20: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:23:20: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:23:22: 26000000 INFO @ Tue, 16 Jun 2020 08:23:22: 30000000 INFO @ Tue, 16 Jun 2020 08:23:22: #2 number of paired peaks: 13 WARNING @ Tue, 16 Jun 2020 08:23:22: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:23:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX1674087/SRX1674087.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674087/SRX1674087.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674087/SRX1674087.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674087/SRX1674087.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:23:29: 27000000 INFO @ Tue, 16 Jun 2020 08:23:29: 31000000 INFO @ Tue, 16 Jun 2020 08:23:37: 28000000 INFO @ Tue, 16 Jun 2020 08:23:37: 32000000 INFO @ Tue, 16 Jun 2020 08:23:44: 33000000 INFO @ Tue, 16 Jun 2020 08:23:44: 29000000 INFO @ Tue, 16 Jun 2020 08:23:51: 34000000 INFO @ Tue, 16 Jun 2020 08:23:51: 30000000 INFO @ Tue, 16 Jun 2020 08:23:55: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 08:23:55: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 08:23:55: #1 total tags in treatment: 34604574 INFO @ Tue, 16 Jun 2020 08:23:55: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:23:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:23:56: #1 tags after filtering in treatment: 34604574 INFO @ Tue, 16 Jun 2020 08:23:56: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:23:56: #1 finished! INFO @ Tue, 16 Jun 2020 08:23:56: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:23:56: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:23:58: #2 number of paired peaks: 13 WARNING @ Tue, 16 Jun 2020 08:23:58: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:23:58: Process for pairing-model is terminated! INFO @ Tue, 16 Jun 2020 08:23:59: 31000000 BigWig に変換しました。 cut: /home/okishinya/chipatlas/results/ce11/SRX1674087/SRX1674087.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674087/SRX1674087.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674087/SRX1674087.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674087/SRX1674087.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:24:06: 32000000 INFO @ Tue, 16 Jun 2020 08:24:12: 33000000 INFO @ Tue, 16 Jun 2020 08:24:19: 34000000 INFO @ Tue, 16 Jun 2020 08:24:23: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 08:24:23: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 08:24:23: #1 total tags in treatment: 34604574 INFO @ Tue, 16 Jun 2020 08:24:23: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:24:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:24:24: #1 tags after filtering in treatment: 34604574 INFO @ Tue, 16 Jun 2020 08:24:24: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:24:24: #1 finished! INFO @ Tue, 16 Jun 2020 08:24:24: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:24:24: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:24:26: #2 number of paired peaks: 13 WARNING @ Tue, 16 Jun 2020 08:24:26: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:24:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX1674087/SRX1674087.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674087/SRX1674087.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674087/SRX1674087.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674087/SRX1674087.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling