Job ID = 6366467 SRX = SRX147620 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T22:50:11 prefetch.2.10.7: 1) Downloading 'SRR496297'... 2020-06-15T22:50:11 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T22:50:36 prefetch.2.10.7: HTTPS download succeed 2020-06-15T22:50:36 prefetch.2.10.7: 'SRR496297' is valid 2020-06-15T22:50:36 prefetch.2.10.7: 1) 'SRR496297' was downloaded successfully Read 3568932 spots for SRR496297/SRR496297.sra Written 3568932 spots for SRR496297/SRR496297.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:30 3568932 reads; of these: 3568932 (100.00%) were unpaired; of these: 1017676 (28.51%) aligned 0 times 2157234 (60.44%) aligned exactly 1 time 394022 (11.04%) aligned >1 times 71.49% overall alignment rate Time searching: 00:00:30 Overall time: 00:00:30 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 116062 / 2551256 = 0.0455 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:52:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX147620/SRX147620.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX147620/SRX147620.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX147620/SRX147620.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX147620/SRX147620.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:52:20: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:52:20: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:52:25: 1000000 INFO @ Tue, 16 Jun 2020 07:52:30: 2000000 INFO @ Tue, 16 Jun 2020 07:52:32: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:52:32: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:52:32: #1 total tags in treatment: 2435194 INFO @ Tue, 16 Jun 2020 07:52:32: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:52:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:52:32: #1 tags after filtering in treatment: 2435194 INFO @ Tue, 16 Jun 2020 07:52:32: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:52:32: #1 finished! INFO @ Tue, 16 Jun 2020 07:52:32: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:52:32: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:52:32: #2 number of paired peaks: 429 WARNING @ Tue, 16 Jun 2020 07:52:32: Fewer paired peaks (429) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 429 pairs to build model! INFO @ Tue, 16 Jun 2020 07:52:32: start model_add_line... INFO @ Tue, 16 Jun 2020 07:52:32: start X-correlation... INFO @ Tue, 16 Jun 2020 07:52:33: end of X-cor INFO @ Tue, 16 Jun 2020 07:52:33: #2 finished! INFO @ Tue, 16 Jun 2020 07:52:33: #2 predicted fragment length is 33 bps INFO @ Tue, 16 Jun 2020 07:52:33: #2 alternative fragment length(s) may be 4,33,491,556,562 bps INFO @ Tue, 16 Jun 2020 07:52:33: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX147620/SRX147620.05_model.r WARNING @ Tue, 16 Jun 2020 07:52:33: #2 Since the d (33) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 07:52:33: #2 You may need to consider one of the other alternative d(s): 4,33,491,556,562 WARNING @ Tue, 16 Jun 2020 07:52:33: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 07:52:33: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:52:33: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:52:38: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:52:41: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX147620/SRX147620.05_peaks.xls INFO @ Tue, 16 Jun 2020 07:52:41: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX147620/SRX147620.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:52:41: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX147620/SRX147620.05_summits.bed INFO @ Tue, 16 Jun 2020 07:52:41: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (265 records, 4 fields): 1 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:52:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX147620/SRX147620.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX147620/SRX147620.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX147620/SRX147620.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX147620/SRX147620.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:52:50: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:52:50: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:52:55: 1000000 INFO @ Tue, 16 Jun 2020 07:53:00: 2000000 INFO @ Tue, 16 Jun 2020 07:53:02: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:53:02: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:53:02: #1 total tags in treatment: 2435194 INFO @ Tue, 16 Jun 2020 07:53:02: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:53:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:53:02: #1 tags after filtering in treatment: 2435194 INFO @ Tue, 16 Jun 2020 07:53:02: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:53:02: #1 finished! INFO @ Tue, 16 Jun 2020 07:53:02: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:53:02: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:53:02: #2 number of paired peaks: 429 WARNING @ Tue, 16 Jun 2020 07:53:02: Fewer paired peaks (429) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 429 pairs to build model! INFO @ Tue, 16 Jun 2020 07:53:02: start model_add_line... INFO @ Tue, 16 Jun 2020 07:53:02: start X-correlation... INFO @ Tue, 16 Jun 2020 07:53:02: end of X-cor INFO @ Tue, 16 Jun 2020 07:53:02: #2 finished! INFO @ Tue, 16 Jun 2020 07:53:02: #2 predicted fragment length is 33 bps INFO @ Tue, 16 Jun 2020 07:53:02: #2 alternative fragment length(s) may be 4,33,491,556,562 bps INFO @ Tue, 16 Jun 2020 07:53:02: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX147620/SRX147620.10_model.r WARNING @ Tue, 16 Jun 2020 07:53:02: #2 Since the d (33) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 07:53:02: #2 You may need to consider one of the other alternative d(s): 4,33,491,556,562 WARNING @ Tue, 16 Jun 2020 07:53:02: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 07:53:02: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:53:02: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:53:07: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:53:10: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX147620/SRX147620.10_peaks.xls INFO @ Tue, 16 Jun 2020 07:53:10: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX147620/SRX147620.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:53:10: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX147620/SRX147620.10_summits.bed INFO @ Tue, 16 Jun 2020 07:53:10: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (93 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:53:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX147620/SRX147620.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX147620/SRX147620.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX147620/SRX147620.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX147620/SRX147620.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:53:20: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:53:20: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:53:26: 1000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 07:53:33: 2000000 INFO @ Tue, 16 Jun 2020 07:53:36: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:53:36: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:53:36: #1 total tags in treatment: 2435194 INFO @ Tue, 16 Jun 2020 07:53:36: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:53:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:53:36: #1 tags after filtering in treatment: 2435194 INFO @ Tue, 16 Jun 2020 07:53:36: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:53:36: #1 finished! INFO @ Tue, 16 Jun 2020 07:53:36: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:53:36: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:53:36: #2 number of paired peaks: 429 WARNING @ Tue, 16 Jun 2020 07:53:36: Fewer paired peaks (429) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 429 pairs to build model! INFO @ Tue, 16 Jun 2020 07:53:36: start model_add_line... INFO @ Tue, 16 Jun 2020 07:53:36: start X-correlation... INFO @ Tue, 16 Jun 2020 07:53:36: end of X-cor INFO @ Tue, 16 Jun 2020 07:53:36: #2 finished! INFO @ Tue, 16 Jun 2020 07:53:36: #2 predicted fragment length is 33 bps INFO @ Tue, 16 Jun 2020 07:53:36: #2 alternative fragment length(s) may be 4,33,491,556,562 bps INFO @ Tue, 16 Jun 2020 07:53:36: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX147620/SRX147620.20_model.r WARNING @ Tue, 16 Jun 2020 07:53:36: #2 Since the d (33) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 07:53:36: #2 You may need to consider one of the other alternative d(s): 4,33,491,556,562 WARNING @ Tue, 16 Jun 2020 07:53:36: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 07:53:36: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:53:36: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 07:53:42: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:53:44: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX147620/SRX147620.20_peaks.xls INFO @ Tue, 16 Jun 2020 07:53:44: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX147620/SRX147620.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:53:44: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX147620/SRX147620.20_summits.bed INFO @ Tue, 16 Jun 2020 07:53:44: Done! pass1 - making usageList (5 chroms): 1 millis pass2 - checking and writing primary data (16 records, 4 fields): 1 millis CompletedMACS2peakCalling