Job ID = 6366465 SRX = SRX147618 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T22:50:11 prefetch.2.10.7: 1) Downloading 'SRR496295'... 2020-06-15T22:50:11 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T22:50:43 prefetch.2.10.7: HTTPS download succeed 2020-06-15T22:50:43 prefetch.2.10.7: 'SRR496295' is valid 2020-06-15T22:50:43 prefetch.2.10.7: 1) 'SRR496295' was downloaded successfully Read 5324899 spots for SRR496295/SRR496295.sra Written 5324899 spots for SRR496295/SRR496295.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:35 5324899 reads; of these: 5324899 (100.00%) were unpaired; of these: 2538567 (47.67%) aligned 0 times 2331199 (43.78%) aligned exactly 1 time 455133 (8.55%) aligned >1 times 52.33% overall alignment rate Time searching: 00:00:35 Overall time: 00:00:35 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 273997 / 2786332 = 0.0983 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:52:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX147618/SRX147618.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX147618/SRX147618.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX147618/SRX147618.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX147618/SRX147618.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:52:39: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:52:39: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:52:45: 1000000 INFO @ Tue, 16 Jun 2020 07:52:50: 2000000 INFO @ Tue, 16 Jun 2020 07:52:53: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:52:53: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:52:53: #1 total tags in treatment: 2512335 INFO @ Tue, 16 Jun 2020 07:52:53: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:52:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:52:53: #1 tags after filtering in treatment: 2512335 INFO @ Tue, 16 Jun 2020 07:52:53: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:52:53: #1 finished! INFO @ Tue, 16 Jun 2020 07:52:53: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:52:53: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:52:53: #2 number of paired peaks: 521 WARNING @ Tue, 16 Jun 2020 07:52:53: Fewer paired peaks (521) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 521 pairs to build model! INFO @ Tue, 16 Jun 2020 07:52:53: start model_add_line... INFO @ Tue, 16 Jun 2020 07:52:53: start X-correlation... INFO @ Tue, 16 Jun 2020 07:52:53: end of X-cor INFO @ Tue, 16 Jun 2020 07:52:53: #2 finished! INFO @ Tue, 16 Jun 2020 07:52:53: #2 predicted fragment length is 74 bps INFO @ Tue, 16 Jun 2020 07:52:53: #2 alternative fragment length(s) may be 4,53,74,539,587 bps INFO @ Tue, 16 Jun 2020 07:52:53: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX147618/SRX147618.05_model.r INFO @ Tue, 16 Jun 2020 07:52:53: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:52:53: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:52:59: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:53:02: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX147618/SRX147618.05_peaks.xls INFO @ Tue, 16 Jun 2020 07:53:02: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX147618/SRX147618.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:53:02: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX147618/SRX147618.05_summits.bed INFO @ Tue, 16 Jun 2020 07:53:02: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (407 records, 4 fields): 2 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:53:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX147618/SRX147618.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX147618/SRX147618.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX147618/SRX147618.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX147618/SRX147618.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:53:09: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:53:09: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:53:15: 1000000 INFO @ Tue, 16 Jun 2020 07:53:20: 2000000 INFO @ Tue, 16 Jun 2020 07:53:23: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:53:23: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:53:23: #1 total tags in treatment: 2512335 INFO @ Tue, 16 Jun 2020 07:53:23: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:53:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:53:23: #1 tags after filtering in treatment: 2512335 INFO @ Tue, 16 Jun 2020 07:53:23: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:53:23: #1 finished! INFO @ Tue, 16 Jun 2020 07:53:23: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:53:23: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:53:23: #2 number of paired peaks: 521 WARNING @ Tue, 16 Jun 2020 07:53:23: Fewer paired peaks (521) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 521 pairs to build model! INFO @ Tue, 16 Jun 2020 07:53:23: start model_add_line... INFO @ Tue, 16 Jun 2020 07:53:23: start X-correlation... INFO @ Tue, 16 Jun 2020 07:53:23: end of X-cor INFO @ Tue, 16 Jun 2020 07:53:23: #2 finished! INFO @ Tue, 16 Jun 2020 07:53:23: #2 predicted fragment length is 74 bps INFO @ Tue, 16 Jun 2020 07:53:23: #2 alternative fragment length(s) may be 4,53,74,539,587 bps INFO @ Tue, 16 Jun 2020 07:53:23: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX147618/SRX147618.10_model.r INFO @ Tue, 16 Jun 2020 07:53:23: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:53:23: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:53:29: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:53:32: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX147618/SRX147618.10_peaks.xls INFO @ Tue, 16 Jun 2020 07:53:32: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX147618/SRX147618.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:53:32: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX147618/SRX147618.10_summits.bed INFO @ Tue, 16 Jun 2020 07:53:32: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (190 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:53:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX147618/SRX147618.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX147618/SRX147618.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX147618/SRX147618.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX147618/SRX147618.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:53:39: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:53:39: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:53:45: 1000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 07:53:51: 2000000 INFO @ Tue, 16 Jun 2020 07:53:53: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:53:53: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:53:53: #1 total tags in treatment: 2512335 INFO @ Tue, 16 Jun 2020 07:53:53: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:53:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:53:53: #1 tags after filtering in treatment: 2512335 INFO @ Tue, 16 Jun 2020 07:53:53: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:53:53: #1 finished! INFO @ Tue, 16 Jun 2020 07:53:53: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:53:53: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:53:53: #2 number of paired peaks: 521 WARNING @ Tue, 16 Jun 2020 07:53:53: Fewer paired peaks (521) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 521 pairs to build model! INFO @ Tue, 16 Jun 2020 07:53:53: start model_add_line... INFO @ Tue, 16 Jun 2020 07:53:53: start X-correlation... INFO @ Tue, 16 Jun 2020 07:53:53: end of X-cor INFO @ Tue, 16 Jun 2020 07:53:53: #2 finished! INFO @ Tue, 16 Jun 2020 07:53:53: #2 predicted fragment length is 74 bps INFO @ Tue, 16 Jun 2020 07:53:53: #2 alternative fragment length(s) may be 4,53,74,539,587 bps INFO @ Tue, 16 Jun 2020 07:53:53: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX147618/SRX147618.20_model.r INFO @ Tue, 16 Jun 2020 07:53:53: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:53:53: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 07:53:59: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:54:02: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX147618/SRX147618.20_peaks.xls INFO @ Tue, 16 Jun 2020 07:54:02: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX147618/SRX147618.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:54:02: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX147618/SRX147618.20_summits.bed INFO @ Tue, 16 Jun 2020 07:54:02: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (60 records, 4 fields): 1 millis CompletedMACS2peakCalling