Job ID = 6366453 SRX = SRX146517 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T22:48:37 prefetch.2.10.7: 1) Downloading 'SRR494685'... 2020-06-15T22:48:37 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T22:49:07 prefetch.2.10.7: HTTPS download succeed 2020-06-15T22:49:08 prefetch.2.10.7: 'SRR494685' is valid 2020-06-15T22:49:08 prefetch.2.10.7: 1) 'SRR494685' was downloaded successfully Read 2598529 spots for SRR494685/SRR494685.sra Written 2598529 spots for SRR494685/SRR494685.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:17 2598529 reads; of these: 2598529 (100.00%) were unpaired; of these: 1142465 (43.97%) aligned 0 times 1233498 (47.47%) aligned exactly 1 time 222566 (8.57%) aligned >1 times 56.03% overall alignment rate Time searching: 00:00:18 Overall time: 00:00:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 97757 / 1456064 = 0.0671 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:50:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX146517/SRX146517.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX146517/SRX146517.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX146517/SRX146517.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX146517/SRX146517.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:50:21: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:50:21: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:50:26: 1000000 INFO @ Tue, 16 Jun 2020 07:50:28: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:50:28: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:50:28: #1 total tags in treatment: 1358307 INFO @ Tue, 16 Jun 2020 07:50:28: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:50:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:50:28: #1 tags after filtering in treatment: 1358307 INFO @ Tue, 16 Jun 2020 07:50:28: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:50:28: #1 finished! INFO @ Tue, 16 Jun 2020 07:50:28: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:50:28: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:50:28: #2 number of paired peaks: 1185 INFO @ Tue, 16 Jun 2020 07:50:28: start model_add_line... INFO @ Tue, 16 Jun 2020 07:50:28: start X-correlation... INFO @ Tue, 16 Jun 2020 07:50:28: end of X-cor INFO @ Tue, 16 Jun 2020 07:50:28: #2 finished! INFO @ Tue, 16 Jun 2020 07:50:28: #2 predicted fragment length is 119 bps INFO @ Tue, 16 Jun 2020 07:50:28: #2 alternative fragment length(s) may be 119 bps INFO @ Tue, 16 Jun 2020 07:50:28: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX146517/SRX146517.05_model.r INFO @ Tue, 16 Jun 2020 07:50:28: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:50:28: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:50:32: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:50:33: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX146517/SRX146517.05_peaks.xls INFO @ Tue, 16 Jun 2020 07:50:33: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX146517/SRX146517.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:50:33: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX146517/SRX146517.05_summits.bed INFO @ Tue, 16 Jun 2020 07:50:33: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (1561 records, 4 fields): 3 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:50:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX146517/SRX146517.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX146517/SRX146517.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX146517/SRX146517.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX146517/SRX146517.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:50:51: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:50:51: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:50:56: 1000000 INFO @ Tue, 16 Jun 2020 07:50:58: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:50:58: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:50:58: #1 total tags in treatment: 1358307 INFO @ Tue, 16 Jun 2020 07:50:58: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:50:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:50:58: #1 tags after filtering in treatment: 1358307 INFO @ Tue, 16 Jun 2020 07:50:58: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:50:58: #1 finished! INFO @ Tue, 16 Jun 2020 07:50:58: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:50:58: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:50:58: #2 number of paired peaks: 1185 INFO @ Tue, 16 Jun 2020 07:50:58: start model_add_line... INFO @ Tue, 16 Jun 2020 07:50:58: start X-correlation... INFO @ Tue, 16 Jun 2020 07:50:58: end of X-cor INFO @ Tue, 16 Jun 2020 07:50:58: #2 finished! INFO @ Tue, 16 Jun 2020 07:50:58: #2 predicted fragment length is 119 bps INFO @ Tue, 16 Jun 2020 07:50:58: #2 alternative fragment length(s) may be 119 bps INFO @ Tue, 16 Jun 2020 07:50:58: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX146517/SRX146517.10_model.r INFO @ Tue, 16 Jun 2020 07:50:58: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:50:58: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:51:02: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:51:03: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX146517/SRX146517.10_peaks.xls INFO @ Tue, 16 Jun 2020 07:51:03: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX146517/SRX146517.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:51:03: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX146517/SRX146517.10_summits.bed INFO @ Tue, 16 Jun 2020 07:51:03: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (940 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:51:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX146517/SRX146517.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX146517/SRX146517.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX146517/SRX146517.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX146517/SRX146517.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:51:21: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:51:21: #1 read treatment tags... BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 07:51:27: 1000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 07:51:29: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:51:29: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:51:29: #1 total tags in treatment: 1358307 INFO @ Tue, 16 Jun 2020 07:51:29: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:51:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:51:29: #1 tags after filtering in treatment: 1358307 INFO @ Tue, 16 Jun 2020 07:51:29: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:51:29: #1 finished! INFO @ Tue, 16 Jun 2020 07:51:29: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:51:29: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:51:29: #2 number of paired peaks: 1185 INFO @ Tue, 16 Jun 2020 07:51:29: start model_add_line... INFO @ Tue, 16 Jun 2020 07:51:29: start X-correlation... INFO @ Tue, 16 Jun 2020 07:51:29: end of X-cor INFO @ Tue, 16 Jun 2020 07:51:29: #2 finished! INFO @ Tue, 16 Jun 2020 07:51:29: #2 predicted fragment length is 119 bps INFO @ Tue, 16 Jun 2020 07:51:29: #2 alternative fragment length(s) may be 119 bps INFO @ Tue, 16 Jun 2020 07:51:29: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX146517/SRX146517.20_model.r INFO @ Tue, 16 Jun 2020 07:51:29: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:51:29: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:51:32: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:51:34: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX146517/SRX146517.20_peaks.xls INFO @ Tue, 16 Jun 2020 07:51:34: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX146517/SRX146517.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:51:34: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX146517/SRX146517.20_summits.bed INFO @ Tue, 16 Jun 2020 07:51:34: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (591 records, 4 fields): 2 millis CompletedMACS2peakCalling