Job ID = 6366417 SRX = SRX146424 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T22:43:22 prefetch.2.10.7: 1) Downloading 'SRR494585'... 2020-06-15T22:43:22 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T22:43:53 prefetch.2.10.7: HTTPS download succeed 2020-06-15T22:43:53 prefetch.2.10.7: 'SRR494585' is valid 2020-06-15T22:43:53 prefetch.2.10.7: 1) 'SRR494585' was downloaded successfully Read 4285296 spots for SRR494585/SRR494585.sra Written 4285296 spots for SRR494585/SRR494585.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:26 4285296 reads; of these: 4285296 (100.00%) were unpaired; of these: 2836173 (66.18%) aligned 0 times 1193637 (27.85%) aligned exactly 1 time 255486 (5.96%) aligned >1 times 33.82% overall alignment rate Time searching: 00:00:26 Overall time: 00:00:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 386860 / 1449123 = 0.2670 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:45:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX146424/SRX146424.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX146424/SRX146424.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX146424/SRX146424.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX146424/SRX146424.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:45:21: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:45:21: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:45:28: 1000000 INFO @ Tue, 16 Jun 2020 07:45:28: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:45:28: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:45:28: #1 total tags in treatment: 1062263 INFO @ Tue, 16 Jun 2020 07:45:28: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:45:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:45:28: #1 tags after filtering in treatment: 1062263 INFO @ Tue, 16 Jun 2020 07:45:28: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:45:28: #1 finished! INFO @ Tue, 16 Jun 2020 07:45:28: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:45:28: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:45:29: #2 number of paired peaks: 1080 INFO @ Tue, 16 Jun 2020 07:45:29: start model_add_line... INFO @ Tue, 16 Jun 2020 07:45:29: start X-correlation... INFO @ Tue, 16 Jun 2020 07:45:29: end of X-cor INFO @ Tue, 16 Jun 2020 07:45:29: #2 finished! INFO @ Tue, 16 Jun 2020 07:45:29: #2 predicted fragment length is 147 bps INFO @ Tue, 16 Jun 2020 07:45:29: #2 alternative fragment length(s) may be 147 bps INFO @ Tue, 16 Jun 2020 07:45:29: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX146424/SRX146424.05_model.r INFO @ Tue, 16 Jun 2020 07:45:29: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:45:29: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:45:31: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:45:33: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX146424/SRX146424.05_peaks.xls INFO @ Tue, 16 Jun 2020 07:45:33: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX146424/SRX146424.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:45:33: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX146424/SRX146424.05_summits.bed INFO @ Tue, 16 Jun 2020 07:45:33: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (1086 records, 4 fields): 3 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:45:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX146424/SRX146424.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX146424/SRX146424.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX146424/SRX146424.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX146424/SRX146424.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:45:52: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:45:52: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:45:58: 1000000 INFO @ Tue, 16 Jun 2020 07:45:58: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:45:58: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:45:58: #1 total tags in treatment: 1062263 INFO @ Tue, 16 Jun 2020 07:45:58: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:45:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:45:58: #1 tags after filtering in treatment: 1062263 INFO @ Tue, 16 Jun 2020 07:45:58: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:45:58: #1 finished! INFO @ Tue, 16 Jun 2020 07:45:58: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:45:58: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:45:58: #2 number of paired peaks: 1080 INFO @ Tue, 16 Jun 2020 07:45:58: start model_add_line... INFO @ Tue, 16 Jun 2020 07:45:58: start X-correlation... INFO @ Tue, 16 Jun 2020 07:45:58: end of X-cor INFO @ Tue, 16 Jun 2020 07:45:58: #2 finished! INFO @ Tue, 16 Jun 2020 07:45:58: #2 predicted fragment length is 147 bps INFO @ Tue, 16 Jun 2020 07:45:58: #2 alternative fragment length(s) may be 147 bps INFO @ Tue, 16 Jun 2020 07:45:58: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX146424/SRX146424.10_model.r INFO @ Tue, 16 Jun 2020 07:45:58: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:45:58: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:46:01: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:46:02: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX146424/SRX146424.10_peaks.xls INFO @ Tue, 16 Jun 2020 07:46:02: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX146424/SRX146424.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:46:02: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX146424/SRX146424.10_summits.bed INFO @ Tue, 16 Jun 2020 07:46:02: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (565 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:46:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX146424/SRX146424.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX146424/SRX146424.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX146424/SRX146424.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX146424/SRX146424.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:46:21: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:46:21: #1 read treatment tags... BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 07:46:27: 1000000 INFO @ Tue, 16 Jun 2020 07:46:27: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:46:27: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:46:27: #1 total tags in treatment: 1062263 INFO @ Tue, 16 Jun 2020 07:46:27: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:46:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:46:27: #1 tags after filtering in treatment: 1062263 INFO @ Tue, 16 Jun 2020 07:46:27: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:46:27: #1 finished! INFO @ Tue, 16 Jun 2020 07:46:27: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:46:27: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:46:28: #2 number of paired peaks: 1080 INFO @ Tue, 16 Jun 2020 07:46:28: start model_add_line... INFO @ Tue, 16 Jun 2020 07:46:28: start X-correlation... INFO @ Tue, 16 Jun 2020 07:46:28: end of X-cor INFO @ Tue, 16 Jun 2020 07:46:28: #2 finished! INFO @ Tue, 16 Jun 2020 07:46:28: #2 predicted fragment length is 147 bps INFO @ Tue, 16 Jun 2020 07:46:28: #2 alternative fragment length(s) may be 147 bps INFO @ Tue, 16 Jun 2020 07:46:28: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX146424/SRX146424.20_model.r INFO @ Tue, 16 Jun 2020 07:46:28: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:46:28: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:46:30: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:46:32: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX146424/SRX146424.20_peaks.xls INFO @ Tue, 16 Jun 2020 07:46:32: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX146424/SRX146424.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:46:32: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX146424/SRX146424.20_summits.bed INFO @ Tue, 16 Jun 2020 07:46:32: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (227 records, 4 fields): 2 millis CompletedMACS2peakCalling