Job ID = 6366322 SRX = SRX1132918 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T22:35:51 prefetch.2.10.7: 1) Downloading 'SRR2144367'... 2020-06-15T22:35:51 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T22:38:07 prefetch.2.10.7: HTTPS download succeed 2020-06-15T22:38:07 prefetch.2.10.7: 1) 'SRR2144367' was downloaded successfully Read 25944757 spots for SRR2144367/SRR2144367.sra Written 25944757 spots for SRR2144367/SRR2144367.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:48 25944757 reads; of these: 25944757 (100.00%) were unpaired; of these: 1883157 (7.26%) aligned 0 times 20023307 (77.18%) aligned exactly 1 time 4038293 (15.56%) aligned >1 times 92.74% overall alignment rate Time searching: 00:07:48 Overall time: 00:07:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 10226780 / 24061600 = 0.4250 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:53:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1132918/SRX1132918.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1132918/SRX1132918.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1132918/SRX1132918.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1132918/SRX1132918.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:53:40: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:53:40: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:53:46: 1000000 INFO @ Tue, 16 Jun 2020 07:53:52: 2000000 INFO @ Tue, 16 Jun 2020 07:53:58: 3000000 INFO @ Tue, 16 Jun 2020 07:54:04: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:54:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1132918/SRX1132918.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1132918/SRX1132918.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1132918/SRX1132918.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1132918/SRX1132918.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:54:10: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:54:10: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:54:10: 5000000 INFO @ Tue, 16 Jun 2020 07:54:16: 1000000 INFO @ Tue, 16 Jun 2020 07:54:16: 6000000 INFO @ Tue, 16 Jun 2020 07:54:22: 2000000 INFO @ Tue, 16 Jun 2020 07:54:22: 7000000 INFO @ Tue, 16 Jun 2020 07:54:28: 3000000 INFO @ Tue, 16 Jun 2020 07:54:28: 8000000 INFO @ Tue, 16 Jun 2020 07:54:34: 4000000 INFO @ Tue, 16 Jun 2020 07:54:34: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:54:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1132918/SRX1132918.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1132918/SRX1132918.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1132918/SRX1132918.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1132918/SRX1132918.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:54:40: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:54:40: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:54:40: 5000000 INFO @ Tue, 16 Jun 2020 07:54:40: 10000000 INFO @ Tue, 16 Jun 2020 07:54:45: 1000000 INFO @ Tue, 16 Jun 2020 07:54:46: 6000000 INFO @ Tue, 16 Jun 2020 07:54:47: 11000000 INFO @ Tue, 16 Jun 2020 07:54:51: 2000000 INFO @ Tue, 16 Jun 2020 07:54:53: 7000000 INFO @ Tue, 16 Jun 2020 07:54:53: 12000000 INFO @ Tue, 16 Jun 2020 07:54:57: 3000000 INFO @ Tue, 16 Jun 2020 07:54:59: 13000000 INFO @ Tue, 16 Jun 2020 07:54:59: 8000000 INFO @ Tue, 16 Jun 2020 07:55:02: 4000000 INFO @ Tue, 16 Jun 2020 07:55:04: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 07:55:04: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 07:55:04: #1 total tags in treatment: 13834820 INFO @ Tue, 16 Jun 2020 07:55:04: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:55:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:55:04: #1 tags after filtering in treatment: 13834820 INFO @ Tue, 16 Jun 2020 07:55:04: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:55:04: #1 finished! INFO @ Tue, 16 Jun 2020 07:55:04: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:55:04: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:55:05: 9000000 INFO @ Tue, 16 Jun 2020 07:55:05: #2 number of paired peaks: 38 WARNING @ Tue, 16 Jun 2020 07:55:05: Too few paired peaks (38) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 07:55:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX1132918/SRX1132918.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132918/SRX1132918.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132918/SRX1132918.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132918/SRX1132918.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 07:55:08: 5000000 INFO @ Tue, 16 Jun 2020 07:55:11: 10000000 INFO @ Tue, 16 Jun 2020 07:55:14: 6000000 INFO @ Tue, 16 Jun 2020 07:55:17: 11000000 INFO @ Tue, 16 Jun 2020 07:55:19: 7000000 INFO @ Tue, 16 Jun 2020 07:55:24: 12000000 INFO @ Tue, 16 Jun 2020 07:55:25: 8000000 INFO @ Tue, 16 Jun 2020 07:55:30: 13000000 INFO @ Tue, 16 Jun 2020 07:55:30: 9000000 INFO @ Tue, 16 Jun 2020 07:55:35: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 07:55:35: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 07:55:35: #1 total tags in treatment: 13834820 INFO @ Tue, 16 Jun 2020 07:55:35: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:55:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:55:35: #1 tags after filtering in treatment: 13834820 INFO @ Tue, 16 Jun 2020 07:55:35: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:55:35: #1 finished! INFO @ Tue, 16 Jun 2020 07:55:35: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:55:35: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:55:36: 10000000 INFO @ Tue, 16 Jun 2020 07:55:36: #2 number of paired peaks: 38 WARNING @ Tue, 16 Jun 2020 07:55:36: Too few paired peaks (38) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 07:55:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX1132918/SRX1132918.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132918/SRX1132918.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132918/SRX1132918.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132918/SRX1132918.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 07:55:41: 11000000 INFO @ Tue, 16 Jun 2020 07:55:47: 12000000 INFO @ Tue, 16 Jun 2020 07:55:52: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 07:55:57: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 07:55:57: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 07:55:57: #1 total tags in treatment: 13834820 INFO @ Tue, 16 Jun 2020 07:55:57: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:55:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:55:57: #1 tags after filtering in treatment: 13834820 INFO @ Tue, 16 Jun 2020 07:55:57: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:55:57: #1 finished! INFO @ Tue, 16 Jun 2020 07:55:57: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:55:57: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:55:58: #2 number of paired peaks: 38 WARNING @ Tue, 16 Jun 2020 07:55:58: Too few paired peaks (38) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 07:55:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX1132918/SRX1132918.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132918/SRX1132918.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132918/SRX1132918.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132918/SRX1132918.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。