Job ID = 6366321 SRX = SRX1132917 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T22:31:29 prefetch.2.10.7: 1) Downloading 'SRR2144366'... 2020-06-15T22:31:29 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T22:34:37 prefetch.2.10.7: HTTPS download succeed 2020-06-15T22:34:37 prefetch.2.10.7: 1) 'SRR2144366' was downloaded successfully Read 27950500 spots for SRR2144366/SRR2144366.sra Written 27950500 spots for SRR2144366/SRR2144366.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:41 27950500 reads; of these: 27950500 (100.00%) were unpaired; of these: 1009866 (3.61%) aligned 0 times 23423929 (83.81%) aligned exactly 1 time 3516705 (12.58%) aligned >1 times 96.39% overall alignment rate Time searching: 00:08:41 Overall time: 00:08:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 8563203 / 26940634 = 0.3179 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:54:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1132917/SRX1132917.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1132917/SRX1132917.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1132917/SRX1132917.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1132917/SRX1132917.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:54:02: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:54:02: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:54:09: 1000000 INFO @ Tue, 16 Jun 2020 07:54:15: 2000000 INFO @ Tue, 16 Jun 2020 07:54:21: 3000000 INFO @ Tue, 16 Jun 2020 07:54:28: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:54:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1132917/SRX1132917.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1132917/SRX1132917.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1132917/SRX1132917.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1132917/SRX1132917.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:54:32: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:54:32: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:54:34: 5000000 INFO @ Tue, 16 Jun 2020 07:54:39: 1000000 INFO @ Tue, 16 Jun 2020 07:54:41: 6000000 INFO @ Tue, 16 Jun 2020 07:54:46: 2000000 INFO @ Tue, 16 Jun 2020 07:54:48: 7000000 INFO @ Tue, 16 Jun 2020 07:54:53: 3000000 INFO @ Tue, 16 Jun 2020 07:54:55: 8000000 INFO @ Tue, 16 Jun 2020 07:55:00: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:55:02: 9000000 INFO @ Tue, 16 Jun 2020 07:55:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1132917/SRX1132917.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1132917/SRX1132917.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1132917/SRX1132917.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1132917/SRX1132917.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:55:02: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:55:02: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:55:07: 5000000 INFO @ Tue, 16 Jun 2020 07:55:08: 10000000 INFO @ Tue, 16 Jun 2020 07:55:10: 1000000 INFO @ Tue, 16 Jun 2020 07:55:14: 6000000 INFO @ Tue, 16 Jun 2020 07:55:14: 11000000 INFO @ Tue, 16 Jun 2020 07:55:17: 2000000 INFO @ Tue, 16 Jun 2020 07:55:20: 12000000 INFO @ Tue, 16 Jun 2020 07:55:22: 7000000 INFO @ Tue, 16 Jun 2020 07:55:24: 3000000 INFO @ Tue, 16 Jun 2020 07:55:27: 13000000 INFO @ Tue, 16 Jun 2020 07:55:29: 8000000 INFO @ Tue, 16 Jun 2020 07:55:32: 4000000 INFO @ Tue, 16 Jun 2020 07:55:35: 14000000 INFO @ Tue, 16 Jun 2020 07:55:36: 9000000 INFO @ Tue, 16 Jun 2020 07:55:39: 5000000 INFO @ Tue, 16 Jun 2020 07:55:42: 15000000 INFO @ Tue, 16 Jun 2020 07:55:44: 10000000 INFO @ Tue, 16 Jun 2020 07:55:46: 6000000 INFO @ Tue, 16 Jun 2020 07:55:48: 16000000 INFO @ Tue, 16 Jun 2020 07:55:51: 11000000 INFO @ Tue, 16 Jun 2020 07:55:54: 7000000 INFO @ Tue, 16 Jun 2020 07:55:55: 17000000 INFO @ Tue, 16 Jun 2020 07:55:58: 12000000 INFO @ Tue, 16 Jun 2020 07:56:01: 18000000 INFO @ Tue, 16 Jun 2020 07:56:02: 8000000 INFO @ Tue, 16 Jun 2020 07:56:04: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 07:56:04: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 07:56:04: #1 total tags in treatment: 18377431 INFO @ Tue, 16 Jun 2020 07:56:04: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:56:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:56:04: #1 tags after filtering in treatment: 18377431 INFO @ Tue, 16 Jun 2020 07:56:04: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:56:04: #1 finished! INFO @ Tue, 16 Jun 2020 07:56:04: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:56:04: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:56:05: #2 number of paired peaks: 8 WARNING @ Tue, 16 Jun 2020 07:56:05: Too few paired peaks (8) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 07:56:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX1132917/SRX1132917.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132917/SRX1132917.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132917/SRX1132917.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132917/SRX1132917.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 07:56:06: 13000000 INFO @ Tue, 16 Jun 2020 07:56:09: 9000000 INFO @ Tue, 16 Jun 2020 07:56:13: 14000000 INFO @ Tue, 16 Jun 2020 07:56:16: 10000000 INFO @ Tue, 16 Jun 2020 07:56:20: 15000000 INFO @ Tue, 16 Jun 2020 07:56:23: 11000000 INFO @ Tue, 16 Jun 2020 07:56:27: 16000000 INFO @ Tue, 16 Jun 2020 07:56:30: 12000000 INFO @ Tue, 16 Jun 2020 07:56:33: 17000000 INFO @ Tue, 16 Jun 2020 07:56:37: 13000000 INFO @ Tue, 16 Jun 2020 07:56:40: 18000000 INFO @ Tue, 16 Jun 2020 07:56:43: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 07:56:43: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 07:56:43: #1 total tags in treatment: 18377431 INFO @ Tue, 16 Jun 2020 07:56:43: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:56:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:56:43: #1 tags after filtering in treatment: 18377431 INFO @ Tue, 16 Jun 2020 07:56:43: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:56:43: #1 finished! INFO @ Tue, 16 Jun 2020 07:56:43: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:56:43: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:56:44: 14000000 INFO @ Tue, 16 Jun 2020 07:56:44: #2 number of paired peaks: 8 WARNING @ Tue, 16 Jun 2020 07:56:44: Too few paired peaks (8) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 07:56:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX1132917/SRX1132917.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132917/SRX1132917.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132917/SRX1132917.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132917/SRX1132917.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 07:56:50: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 07:56:56: 16000000 INFO @ Tue, 16 Jun 2020 07:57:03: 17000000 INFO @ Tue, 16 Jun 2020 07:57:09: 18000000 INFO @ Tue, 16 Jun 2020 07:57:11: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 07:57:11: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 07:57:11: #1 total tags in treatment: 18377431 INFO @ Tue, 16 Jun 2020 07:57:11: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:57:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:57:12: #1 tags after filtering in treatment: 18377431 INFO @ Tue, 16 Jun 2020 07:57:12: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:57:12: #1 finished! INFO @ Tue, 16 Jun 2020 07:57:12: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:57:12: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:57:13: #2 number of paired peaks: 8 WARNING @ Tue, 16 Jun 2020 07:57:13: Too few paired peaks (8) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 07:57:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX1132917/SRX1132917.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132917/SRX1132917.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132917/SRX1132917.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132917/SRX1132917.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。