Job ID = 6366320 SRX = SRX1132916 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T22:37:51 prefetch.2.10.7: 1) Downloading 'SRR2144365'... 2020-06-15T22:37:51 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T22:42:11 prefetch.2.10.7: HTTPS download succeed 2020-06-15T22:42:11 prefetch.2.10.7: 1) 'SRR2144365' was downloaded successfully Read 29558379 spots for SRR2144365/SRR2144365.sra Written 29558379 spots for SRR2144365/SRR2144365.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:17 29558379 reads; of these: 29558379 (100.00%) were unpaired; of these: 3458594 (11.70%) aligned 0 times 21937818 (74.22%) aligned exactly 1 time 4161967 (14.08%) aligned >1 times 88.30% overall alignment rate Time searching: 00:08:17 Overall time: 00:08:17 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 10167581 / 26099785 = 0.3896 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:58:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1132916/SRX1132916.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1132916/SRX1132916.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1132916/SRX1132916.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1132916/SRX1132916.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:58:39: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:58:39: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:58:44: 1000000 INFO @ Tue, 16 Jun 2020 07:58:50: 2000000 INFO @ Tue, 16 Jun 2020 07:58:55: 3000000 INFO @ Tue, 16 Jun 2020 07:59:01: 4000000 INFO @ Tue, 16 Jun 2020 07:59:06: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:59:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1132916/SRX1132916.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1132916/SRX1132916.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1132916/SRX1132916.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1132916/SRX1132916.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:59:09: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:59:09: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:59:12: 6000000 INFO @ Tue, 16 Jun 2020 07:59:15: 1000000 INFO @ Tue, 16 Jun 2020 07:59:18: 7000000 INFO @ Tue, 16 Jun 2020 07:59:21: 2000000 INFO @ Tue, 16 Jun 2020 07:59:24: 8000000 INFO @ Tue, 16 Jun 2020 07:59:27: 3000000 INFO @ Tue, 16 Jun 2020 07:59:30: 9000000 INFO @ Tue, 16 Jun 2020 07:59:32: 4000000 INFO @ Tue, 16 Jun 2020 07:59:35: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:59:38: 5000000 INFO @ Tue, 16 Jun 2020 07:59:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1132916/SRX1132916.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1132916/SRX1132916.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1132916/SRX1132916.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1132916/SRX1132916.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:59:39: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:59:39: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:59:41: 11000000 INFO @ Tue, 16 Jun 2020 07:59:44: 6000000 INFO @ Tue, 16 Jun 2020 07:59:45: 1000000 INFO @ Tue, 16 Jun 2020 07:59:47: 12000000 INFO @ Tue, 16 Jun 2020 07:59:50: 7000000 INFO @ Tue, 16 Jun 2020 07:59:51: 2000000 INFO @ Tue, 16 Jun 2020 07:59:53: 13000000 INFO @ Tue, 16 Jun 2020 07:59:56: 8000000 INFO @ Tue, 16 Jun 2020 07:59:57: 3000000 INFO @ Tue, 16 Jun 2020 08:00:00: 14000000 INFO @ Tue, 16 Jun 2020 08:00:02: 9000000 INFO @ Tue, 16 Jun 2020 08:00:04: 4000000 INFO @ Tue, 16 Jun 2020 08:00:06: 15000000 INFO @ Tue, 16 Jun 2020 08:00:09: 10000000 INFO @ Tue, 16 Jun 2020 08:00:10: 5000000 INFO @ Tue, 16 Jun 2020 08:00:12: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 08:00:12: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 08:00:12: #1 total tags in treatment: 15932204 INFO @ Tue, 16 Jun 2020 08:00:12: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:00:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:00:12: #1 tags after filtering in treatment: 15932204 INFO @ Tue, 16 Jun 2020 08:00:12: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:00:12: #1 finished! INFO @ Tue, 16 Jun 2020 08:00:12: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:00:12: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:00:13: #2 number of paired peaks: 22 WARNING @ Tue, 16 Jun 2020 08:00:13: Too few paired peaks (22) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:00:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX1132916/SRX1132916.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132916/SRX1132916.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132916/SRX1132916.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132916/SRX1132916.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:00:15: 11000000 INFO @ Tue, 16 Jun 2020 08:00:16: 6000000 INFO @ Tue, 16 Jun 2020 08:00:21: 12000000 INFO @ Tue, 16 Jun 2020 08:00:22: 7000000 INFO @ Tue, 16 Jun 2020 08:00:27: 13000000 INFO @ Tue, 16 Jun 2020 08:00:29: 8000000 INFO @ Tue, 16 Jun 2020 08:00:33: 14000000 INFO @ Tue, 16 Jun 2020 08:00:35: 9000000 INFO @ Tue, 16 Jun 2020 08:00:39: 15000000 INFO @ Tue, 16 Jun 2020 08:00:41: 10000000 INFO @ Tue, 16 Jun 2020 08:00:45: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 08:00:45: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 08:00:45: #1 total tags in treatment: 15932204 INFO @ Tue, 16 Jun 2020 08:00:45: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:00:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:00:45: #1 tags after filtering in treatment: 15932204 INFO @ Tue, 16 Jun 2020 08:00:45: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:00:45: #1 finished! INFO @ Tue, 16 Jun 2020 08:00:45: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:00:45: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:00:46: #2 number of paired peaks: 22 WARNING @ Tue, 16 Jun 2020 08:00:46: Too few paired peaks (22) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:00:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX1132916/SRX1132916.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 9 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132916/SRX1132916.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132916/SRX1132916.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132916/SRX1132916.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:00:47: 11000000 INFO @ Tue, 16 Jun 2020 08:00:53: 12000000 INFO @ Tue, 16 Jun 2020 08:00:59: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 08:01:05: 14000000 INFO @ Tue, 16 Jun 2020 08:01:11: 15000000 INFO @ Tue, 16 Jun 2020 08:01:17: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 08:01:17: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 08:01:17: #1 total tags in treatment: 15932204 INFO @ Tue, 16 Jun 2020 08:01:17: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:01:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:01:17: #1 tags after filtering in treatment: 15932204 INFO @ Tue, 16 Jun 2020 08:01:17: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:01:17: #1 finished! INFO @ Tue, 16 Jun 2020 08:01:17: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:01:17: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:01:18: #2 number of paired peaks: 22 WARNING @ Tue, 16 Jun 2020 08:01:18: Too few paired peaks (22) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:01:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX1132916/SRX1132916.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132916/SRX1132916.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132916/SRX1132916.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132916/SRX1132916.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。