Job ID = 6366319 SRX = SRX1132915 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T22:40:51 prefetch.2.10.7: 1) Downloading 'SRR2144364'... 2020-06-15T22:40:51 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T22:43:51 prefetch.2.10.7: HTTPS download succeed 2020-06-15T22:43:51 prefetch.2.10.7: 1) 'SRR2144364' was downloaded successfully Read 22878101 spots for SRR2144364/SRR2144364.sra Written 22878101 spots for SRR2144364/SRR2144364.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:16 22878101 reads; of these: 22878101 (100.00%) were unpaired; of these: 991192 (4.33%) aligned 0 times 18552178 (81.09%) aligned exactly 1 time 3334731 (14.58%) aligned >1 times 95.67% overall alignment rate Time searching: 00:06:16 Overall time: 00:06:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 7996737 / 21886909 = 0.3654 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:56:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1132915/SRX1132915.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1132915/SRX1132915.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1132915/SRX1132915.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1132915/SRX1132915.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:56:50: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:56:50: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:56:57: 1000000 INFO @ Tue, 16 Jun 2020 07:57:03: 2000000 INFO @ Tue, 16 Jun 2020 07:57:10: 3000000 INFO @ Tue, 16 Jun 2020 07:57:17: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:57:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1132915/SRX1132915.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1132915/SRX1132915.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1132915/SRX1132915.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1132915/SRX1132915.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:57:20: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:57:20: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:57:23: 5000000 INFO @ Tue, 16 Jun 2020 07:57:27: 1000000 INFO @ Tue, 16 Jun 2020 07:57:30: 6000000 INFO @ Tue, 16 Jun 2020 07:57:33: 2000000 INFO @ Tue, 16 Jun 2020 07:57:38: 7000000 INFO @ Tue, 16 Jun 2020 07:57:40: 3000000 INFO @ Tue, 16 Jun 2020 07:57:45: 8000000 INFO @ Tue, 16 Jun 2020 07:57:46: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:57:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1132915/SRX1132915.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1132915/SRX1132915.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1132915/SRX1132915.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1132915/SRX1132915.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:57:50: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:57:50: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:57:52: 9000000 INFO @ Tue, 16 Jun 2020 07:57:53: 5000000 INFO @ Tue, 16 Jun 2020 07:57:57: 1000000 INFO @ Tue, 16 Jun 2020 07:57:59: 10000000 INFO @ Tue, 16 Jun 2020 07:57:59: 6000000 INFO @ Tue, 16 Jun 2020 07:58:03: 2000000 INFO @ Tue, 16 Jun 2020 07:58:06: 7000000 INFO @ Tue, 16 Jun 2020 07:58:06: 11000000 INFO @ Tue, 16 Jun 2020 07:58:10: 3000000 INFO @ Tue, 16 Jun 2020 07:58:12: 8000000 INFO @ Tue, 16 Jun 2020 07:58:13: 12000000 INFO @ Tue, 16 Jun 2020 07:58:16: 4000000 INFO @ Tue, 16 Jun 2020 07:58:19: 9000000 INFO @ Tue, 16 Jun 2020 07:58:20: 13000000 INFO @ Tue, 16 Jun 2020 07:58:23: 5000000 INFO @ Tue, 16 Jun 2020 07:58:25: 10000000 INFO @ Tue, 16 Jun 2020 07:58:26: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 07:58:26: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 07:58:26: #1 total tags in treatment: 13890172 INFO @ Tue, 16 Jun 2020 07:58:26: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:58:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:58:26: #1 tags after filtering in treatment: 13890172 INFO @ Tue, 16 Jun 2020 07:58:26: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:58:26: #1 finished! INFO @ Tue, 16 Jun 2020 07:58:26: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:58:26: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:58:27: #2 number of paired peaks: 29 WARNING @ Tue, 16 Jun 2020 07:58:27: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 07:58:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX1132915/SRX1132915.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132915/SRX1132915.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132915/SRX1132915.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132915/SRX1132915.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 07:58:29: 6000000 INFO @ Tue, 16 Jun 2020 07:58:31: 11000000 INFO @ Tue, 16 Jun 2020 07:58:35: 7000000 INFO @ Tue, 16 Jun 2020 07:58:37: 12000000 INFO @ Tue, 16 Jun 2020 07:58:41: 8000000 INFO @ Tue, 16 Jun 2020 07:58:43: 13000000 INFO @ Tue, 16 Jun 2020 07:58:47: 9000000 INFO @ Tue, 16 Jun 2020 07:58:48: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 07:58:48: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 07:58:48: #1 total tags in treatment: 13890172 INFO @ Tue, 16 Jun 2020 07:58:48: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:58:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:58:49: #1 tags after filtering in treatment: 13890172 INFO @ Tue, 16 Jun 2020 07:58:49: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:58:49: #1 finished! INFO @ Tue, 16 Jun 2020 07:58:49: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:58:49: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:58:49: #2 number of paired peaks: 29 WARNING @ Tue, 16 Jun 2020 07:58:49: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 07:58:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX1132915/SRX1132915.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132915/SRX1132915.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132915/SRX1132915.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132915/SRX1132915.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 07:58:53: 10000000 INFO @ Tue, 16 Jun 2020 07:58:58: 11000000 INFO @ Tue, 16 Jun 2020 07:59:04: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 07:59:10: 13000000 INFO @ Tue, 16 Jun 2020 07:59:15: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 07:59:15: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 07:59:15: #1 total tags in treatment: 13890172 INFO @ Tue, 16 Jun 2020 07:59:15: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:59:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:59:16: #1 tags after filtering in treatment: 13890172 INFO @ Tue, 16 Jun 2020 07:59:16: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:59:16: #1 finished! INFO @ Tue, 16 Jun 2020 07:59:16: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:59:16: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:59:17: #2 number of paired peaks: 29 WARNING @ Tue, 16 Jun 2020 07:59:17: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 07:59:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX1132915/SRX1132915.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132915/SRX1132915.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132915/SRX1132915.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132915/SRX1132915.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。