Job ID = 6366318 SRX = SRX1132914 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T22:39:51 prefetch.2.10.7: 1) Downloading 'SRR2144363'... 2020-06-15T22:39:51 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T22:42:54 prefetch.2.10.7: HTTPS download succeed 2020-06-15T22:42:54 prefetch.2.10.7: 1) 'SRR2144363' was downloaded successfully Read 24638339 spots for SRR2144363/SRR2144363.sra Written 24638339 spots for SRR2144363/SRR2144363.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:49 24638339 reads; of these: 24638339 (100.00%) were unpaired; of these: 1280523 (5.20%) aligned 0 times 20396506 (82.78%) aligned exactly 1 time 2961310 (12.02%) aligned >1 times 94.80% overall alignment rate Time searching: 00:06:49 Overall time: 00:06:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 7460743 / 23357816 = 0.3194 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:56:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1132914/SRX1132914.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1132914/SRX1132914.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1132914/SRX1132914.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1132914/SRX1132914.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:56:47: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:56:47: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:56:53: 1000000 INFO @ Tue, 16 Jun 2020 07:57:00: 2000000 INFO @ Tue, 16 Jun 2020 07:57:06: 3000000 INFO @ Tue, 16 Jun 2020 07:57:12: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:57:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1132914/SRX1132914.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1132914/SRX1132914.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1132914/SRX1132914.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1132914/SRX1132914.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:57:17: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:57:17: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:57:19: 5000000 INFO @ Tue, 16 Jun 2020 07:57:24: 1000000 INFO @ Tue, 16 Jun 2020 07:57:25: 6000000 INFO @ Tue, 16 Jun 2020 07:57:31: 2000000 INFO @ Tue, 16 Jun 2020 07:57:32: 7000000 INFO @ Tue, 16 Jun 2020 07:57:37: 3000000 INFO @ Tue, 16 Jun 2020 07:57:38: 8000000 INFO @ Tue, 16 Jun 2020 07:57:44: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:57:45: 9000000 INFO @ Tue, 16 Jun 2020 07:57:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1132914/SRX1132914.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1132914/SRX1132914.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1132914/SRX1132914.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1132914/SRX1132914.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:57:47: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:57:47: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:57:51: 5000000 INFO @ Tue, 16 Jun 2020 07:57:52: 10000000 INFO @ Tue, 16 Jun 2020 07:57:54: 1000000 INFO @ Tue, 16 Jun 2020 07:57:58: 6000000 INFO @ Tue, 16 Jun 2020 07:57:59: 11000000 INFO @ Tue, 16 Jun 2020 07:58:01: 2000000 INFO @ Tue, 16 Jun 2020 07:58:04: 7000000 INFO @ Tue, 16 Jun 2020 07:58:06: 12000000 INFO @ Tue, 16 Jun 2020 07:58:08: 3000000 INFO @ Tue, 16 Jun 2020 07:58:11: 8000000 INFO @ Tue, 16 Jun 2020 07:58:13: 13000000 INFO @ Tue, 16 Jun 2020 07:58:15: 4000000 INFO @ Tue, 16 Jun 2020 07:58:19: 9000000 INFO @ Tue, 16 Jun 2020 07:58:20: 14000000 INFO @ Tue, 16 Jun 2020 07:58:22: 5000000 INFO @ Tue, 16 Jun 2020 07:58:26: 10000000 INFO @ Tue, 16 Jun 2020 07:58:27: 15000000 INFO @ Tue, 16 Jun 2020 07:58:29: 6000000 INFO @ Tue, 16 Jun 2020 07:58:32: 11000000 INFO @ Tue, 16 Jun 2020 07:58:33: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 07:58:33: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 07:58:33: #1 total tags in treatment: 15897073 INFO @ Tue, 16 Jun 2020 07:58:33: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:58:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:58:34: #1 tags after filtering in treatment: 15897073 INFO @ Tue, 16 Jun 2020 07:58:34: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:58:34: #1 finished! INFO @ Tue, 16 Jun 2020 07:58:34: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:58:34: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:58:35: #2 number of paired peaks: 9 WARNING @ Tue, 16 Jun 2020 07:58:35: Too few paired peaks (9) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 07:58:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX1132914/SRX1132914.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132914/SRX1132914.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132914/SRX1132914.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132914/SRX1132914.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 07:58:36: 7000000 INFO @ Tue, 16 Jun 2020 07:58:39: 12000000 INFO @ Tue, 16 Jun 2020 07:58:43: 8000000 INFO @ Tue, 16 Jun 2020 07:58:46: 13000000 INFO @ Tue, 16 Jun 2020 07:58:50: 9000000 INFO @ Tue, 16 Jun 2020 07:58:53: 14000000 INFO @ Tue, 16 Jun 2020 07:58:57: 10000000 INFO @ Tue, 16 Jun 2020 07:59:00: 15000000 INFO @ Tue, 16 Jun 2020 07:59:03: 11000000 INFO @ Tue, 16 Jun 2020 07:59:06: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 07:59:06: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 07:59:06: #1 total tags in treatment: 15897073 INFO @ Tue, 16 Jun 2020 07:59:06: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:59:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:59:06: #1 tags after filtering in treatment: 15897073 INFO @ Tue, 16 Jun 2020 07:59:06: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:59:06: #1 finished! INFO @ Tue, 16 Jun 2020 07:59:06: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:59:06: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 07:59:07: #2 number of paired peaks: 9 WARNING @ Tue, 16 Jun 2020 07:59:07: Too few paired peaks (9) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 07:59:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX1132914/SRX1132914.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132914/SRX1132914.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132914/SRX1132914.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132914/SRX1132914.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 07:59:10: 12000000 INFO @ Tue, 16 Jun 2020 07:59:16: 13000000 INFO @ Tue, 16 Jun 2020 07:59:22: 14000000 INFO @ Tue, 16 Jun 2020 07:59:28: 15000000 INFO @ Tue, 16 Jun 2020 07:59:33: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 07:59:33: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 07:59:33: #1 total tags in treatment: 15897073 INFO @ Tue, 16 Jun 2020 07:59:33: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:59:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:59:34: #1 tags after filtering in treatment: 15897073 INFO @ Tue, 16 Jun 2020 07:59:34: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:59:34: #1 finished! INFO @ Tue, 16 Jun 2020 07:59:34: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:59:34: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:59:35: #2 number of paired peaks: 9 WARNING @ Tue, 16 Jun 2020 07:59:35: Too few paired peaks (9) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 07:59:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX1132914/SRX1132914.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132914/SRX1132914.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132914/SRX1132914.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132914/SRX1132914.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。