Job ID = 6366281 SRX = SRX1078706 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T22:31:44 prefetch.2.10.7: 1) Downloading 'SRR2084313'... 2020-06-15T22:31:44 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T22:35:08 prefetch.2.10.7: HTTPS download succeed 2020-06-15T22:35:08 prefetch.2.10.7: 1) 'SRR2084313' was downloaded successfully Read 36011940 spots for SRR2084313/SRR2084313.sra Written 36011940 spots for SRR2084313/SRR2084313.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:19 36011940 reads; of these: 36011940 (100.00%) were unpaired; of these: 662779 (1.84%) aligned 0 times 29504434 (81.93%) aligned exactly 1 time 5844727 (16.23%) aligned >1 times 98.16% overall alignment rate Time searching: 00:08:19 Overall time: 00:08:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 5867614 / 35349161 = 0.1660 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:54:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1078706/SRX1078706.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1078706/SRX1078706.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1078706/SRX1078706.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1078706/SRX1078706.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:54:19: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:54:19: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:54:25: 1000000 INFO @ Tue, 16 Jun 2020 07:54:31: 2000000 INFO @ Tue, 16 Jun 2020 07:54:37: 3000000 INFO @ Tue, 16 Jun 2020 07:54:43: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:54:49: 5000000 INFO @ Tue, 16 Jun 2020 07:54:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1078706/SRX1078706.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1078706/SRX1078706.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1078706/SRX1078706.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1078706/SRX1078706.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:54:49: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:54:49: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:54:56: 6000000 INFO @ Tue, 16 Jun 2020 07:54:57: 1000000 INFO @ Tue, 16 Jun 2020 07:55:03: 7000000 INFO @ Tue, 16 Jun 2020 07:55:04: 2000000 INFO @ Tue, 16 Jun 2020 07:55:10: 8000000 INFO @ Tue, 16 Jun 2020 07:55:12: 3000000 INFO @ Tue, 16 Jun 2020 07:55:17: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:55:19: 4000000 INFO @ Tue, 16 Jun 2020 07:55:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1078706/SRX1078706.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1078706/SRX1078706.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1078706/SRX1078706.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1078706/SRX1078706.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:55:19: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:55:19: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:55:24: 10000000 INFO @ Tue, 16 Jun 2020 07:55:26: 1000000 INFO @ Tue, 16 Jun 2020 07:55:26: 5000000 INFO @ Tue, 16 Jun 2020 07:55:31: 11000000 INFO @ Tue, 16 Jun 2020 07:55:33: 2000000 INFO @ Tue, 16 Jun 2020 07:55:34: 6000000 INFO @ Tue, 16 Jun 2020 07:55:38: 12000000 INFO @ Tue, 16 Jun 2020 07:55:40: 3000000 INFO @ Tue, 16 Jun 2020 07:55:41: 7000000 INFO @ Tue, 16 Jun 2020 07:55:45: 13000000 INFO @ Tue, 16 Jun 2020 07:55:48: 4000000 INFO @ Tue, 16 Jun 2020 07:55:49: 8000000 INFO @ Tue, 16 Jun 2020 07:55:52: 14000000 INFO @ Tue, 16 Jun 2020 07:55:55: 5000000 INFO @ Tue, 16 Jun 2020 07:55:56: 9000000 INFO @ Tue, 16 Jun 2020 07:55:59: 15000000 INFO @ Tue, 16 Jun 2020 07:56:02: 6000000 INFO @ Tue, 16 Jun 2020 07:56:04: 10000000 INFO @ Tue, 16 Jun 2020 07:56:06: 16000000 INFO @ Tue, 16 Jun 2020 07:56:09: 7000000 INFO @ Tue, 16 Jun 2020 07:56:11: 11000000 INFO @ Tue, 16 Jun 2020 07:56:13: 17000000 INFO @ Tue, 16 Jun 2020 07:56:16: 8000000 INFO @ Tue, 16 Jun 2020 07:56:19: 12000000 INFO @ Tue, 16 Jun 2020 07:56:20: 18000000 INFO @ Tue, 16 Jun 2020 07:56:23: 9000000 INFO @ Tue, 16 Jun 2020 07:56:26: 13000000 INFO @ Tue, 16 Jun 2020 07:56:28: 19000000 INFO @ Tue, 16 Jun 2020 07:56:30: 10000000 INFO @ Tue, 16 Jun 2020 07:56:34: 14000000 INFO @ Tue, 16 Jun 2020 07:56:35: 20000000 INFO @ Tue, 16 Jun 2020 07:56:37: 11000000 INFO @ Tue, 16 Jun 2020 07:56:41: 15000000 INFO @ Tue, 16 Jun 2020 07:56:42: 21000000 INFO @ Tue, 16 Jun 2020 07:56:45: 12000000 INFO @ Tue, 16 Jun 2020 07:56:49: 16000000 INFO @ Tue, 16 Jun 2020 07:56:49: 22000000 INFO @ Tue, 16 Jun 2020 07:56:52: 13000000 INFO @ Tue, 16 Jun 2020 07:56:56: 23000000 INFO @ Tue, 16 Jun 2020 07:56:57: 17000000 INFO @ Tue, 16 Jun 2020 07:56:59: 14000000 INFO @ Tue, 16 Jun 2020 07:57:04: 24000000 INFO @ Tue, 16 Jun 2020 07:57:04: 18000000 INFO @ Tue, 16 Jun 2020 07:57:06: 15000000 INFO @ Tue, 16 Jun 2020 07:57:11: 25000000 INFO @ Tue, 16 Jun 2020 07:57:12: 19000000 INFO @ Tue, 16 Jun 2020 07:57:14: 16000000 INFO @ Tue, 16 Jun 2020 07:57:18: 26000000 INFO @ Tue, 16 Jun 2020 07:57:19: 20000000 INFO @ Tue, 16 Jun 2020 07:57:21: 17000000 INFO @ Tue, 16 Jun 2020 07:57:25: 27000000 INFO @ Tue, 16 Jun 2020 07:57:26: 21000000 INFO @ Tue, 16 Jun 2020 07:57:28: 18000000 INFO @ Tue, 16 Jun 2020 07:57:33: 28000000 INFO @ Tue, 16 Jun 2020 07:57:34: 22000000 INFO @ Tue, 16 Jun 2020 07:57:35: 19000000 INFO @ Tue, 16 Jun 2020 07:57:40: 29000000 INFO @ Tue, 16 Jun 2020 07:57:42: 23000000 INFO @ Tue, 16 Jun 2020 07:57:42: 20000000 INFO @ Tue, 16 Jun 2020 07:57:43: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 07:57:43: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 07:57:43: #1 total tags in treatment: 29481547 INFO @ Tue, 16 Jun 2020 07:57:43: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:57:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 07:57:44: #1 tags after filtering in treatment: 29481547 INFO @ Tue, 16 Jun 2020 07:57:44: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:57:44: #1 finished! INFO @ Tue, 16 Jun 2020 07:57:44: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:57:44: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:57:46: #2 number of paired peaks: 86 WARNING @ Tue, 16 Jun 2020 07:57:46: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 07:57:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX1078706/SRX1078706.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1078706/SRX1078706.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1078706/SRX1078706.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1078706/SRX1078706.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 07:57:49: 21000000 INFO @ Tue, 16 Jun 2020 07:57:49: 24000000 INFO @ Tue, 16 Jun 2020 07:57:56: 22000000 INFO @ Tue, 16 Jun 2020 07:57:57: 25000000 INFO @ Tue, 16 Jun 2020 07:58:03: 23000000 INFO @ Tue, 16 Jun 2020 07:58:04: 26000000 INFO @ Tue, 16 Jun 2020 07:58:10: 24000000 INFO @ Tue, 16 Jun 2020 07:58:11: 27000000 INFO @ Tue, 16 Jun 2020 07:58:17: 25000000 INFO @ Tue, 16 Jun 2020 07:58:19: 28000000 INFO @ Tue, 16 Jun 2020 07:58:23: 26000000 INFO @ Tue, 16 Jun 2020 07:58:26: 29000000 INFO @ Tue, 16 Jun 2020 07:58:30: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 07:58:30: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 07:58:30: #1 total tags in treatment: 29481547 INFO @ Tue, 16 Jun 2020 07:58:30: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:58:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:58:30: 27000000 INFO @ Tue, 16 Jun 2020 07:58:30: #1 tags after filtering in treatment: 29481547 INFO @ Tue, 16 Jun 2020 07:58:30: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:58:30: #1 finished! INFO @ Tue, 16 Jun 2020 07:58:30: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:58:30: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:58:32: #2 number of paired peaks: 86 WARNING @ Tue, 16 Jun 2020 07:58:32: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 07:58:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX1078706/SRX1078706.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1078706/SRX1078706.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1078706/SRX1078706.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1078706/SRX1078706.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 07:58:36: 28000000 INFO @ Tue, 16 Jun 2020 07:58:43: 29000000 INFO @ Tue, 16 Jun 2020 07:58:46: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 07:58:46: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 07:58:46: #1 total tags in treatment: 29481547 INFO @ Tue, 16 Jun 2020 07:58:46: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:58:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:58:46: #1 tags after filtering in treatment: 29481547 INFO @ Tue, 16 Jun 2020 07:58:46: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:58:46: #1 finished! INFO @ Tue, 16 Jun 2020 07:58:46: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:58:46: #2 looking for paired plus/minus strand peaks... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 07:58:48: #2 number of paired peaks: 86 WARNING @ Tue, 16 Jun 2020 07:58:48: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 07:58:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX1078706/SRX1078706.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1078706/SRX1078706.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1078706/SRX1078706.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1078706/SRX1078706.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling