Job ID = 6366279 SRX = SRX105313 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T22:38:51 prefetch.2.10.7: 1) Downloading 'SRR364000'... 2020-06-15T22:38:51 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T22:41:18 prefetch.2.10.7: HTTPS download succeed 2020-06-15T22:41:18 prefetch.2.10.7: 1) 'SRR364000' was downloaded successfully Read 45762552 spots for SRR364000/SRR364000.sra Written 45762552 spots for SRR364000/SRR364000.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:36 45762552 reads; of these: 45762552 (100.00%) were unpaired; of these: 10730414 (23.45%) aligned 0 times 28771836 (62.87%) aligned exactly 1 time 6260302 (13.68%) aligned >1 times 76.55% overall alignment rate Time searching: 00:06:36 Overall time: 00:06:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5498825 / 35032138 = 0.1570 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:55:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX105313/SRX105313.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX105313/SRX105313.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX105313/SRX105313.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX105313/SRX105313.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:55:53: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:55:53: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:55:59: 1000000 INFO @ Tue, 16 Jun 2020 07:56:04: 2000000 INFO @ Tue, 16 Jun 2020 07:56:09: 3000000 INFO @ Tue, 16 Jun 2020 07:56:15: 4000000 INFO @ Tue, 16 Jun 2020 07:56:20: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:56:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX105313/SRX105313.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX105313/SRX105313.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX105313/SRX105313.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX105313/SRX105313.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:56:24: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:56:24: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:56:25: 6000000 INFO @ Tue, 16 Jun 2020 07:56:29: 1000000 INFO @ Tue, 16 Jun 2020 07:56:31: 7000000 INFO @ Tue, 16 Jun 2020 07:56:35: 2000000 INFO @ Tue, 16 Jun 2020 07:56:36: 8000000 INFO @ Tue, 16 Jun 2020 07:56:40: 3000000 INFO @ Tue, 16 Jun 2020 07:56:42: 9000000 INFO @ Tue, 16 Jun 2020 07:56:46: 4000000 INFO @ Tue, 16 Jun 2020 07:56:48: 10000000 INFO @ Tue, 16 Jun 2020 07:56:51: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:56:53: 11000000 INFO @ Tue, 16 Jun 2020 07:56:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX105313/SRX105313.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX105313/SRX105313.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX105313/SRX105313.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX105313/SRX105313.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:56:53: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:56:53: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:56:57: 6000000 INFO @ Tue, 16 Jun 2020 07:56:59: 1000000 INFO @ Tue, 16 Jun 2020 07:56:59: 12000000 INFO @ Tue, 16 Jun 2020 07:57:02: 7000000 INFO @ Tue, 16 Jun 2020 07:57:05: 2000000 INFO @ Tue, 16 Jun 2020 07:57:05: 13000000 INFO @ Tue, 16 Jun 2020 07:57:08: 8000000 INFO @ Tue, 16 Jun 2020 07:57:10: 3000000 INFO @ Tue, 16 Jun 2020 07:57:11: 14000000 INFO @ Tue, 16 Jun 2020 07:57:14: 9000000 INFO @ Tue, 16 Jun 2020 07:57:16: 4000000 INFO @ Tue, 16 Jun 2020 07:57:17: 15000000 INFO @ Tue, 16 Jun 2020 07:57:19: 10000000 INFO @ Tue, 16 Jun 2020 07:57:22: 5000000 INFO @ Tue, 16 Jun 2020 07:57:23: 16000000 INFO @ Tue, 16 Jun 2020 07:57:25: 11000000 INFO @ Tue, 16 Jun 2020 07:57:27: 6000000 INFO @ Tue, 16 Jun 2020 07:57:29: 17000000 INFO @ Tue, 16 Jun 2020 07:57:31: 12000000 INFO @ Tue, 16 Jun 2020 07:57:33: 7000000 INFO @ Tue, 16 Jun 2020 07:57:35: 18000000 INFO @ Tue, 16 Jun 2020 07:57:37: 13000000 INFO @ Tue, 16 Jun 2020 07:57:39: 8000000 INFO @ Tue, 16 Jun 2020 07:57:41: 19000000 INFO @ Tue, 16 Jun 2020 07:57:42: 14000000 INFO @ Tue, 16 Jun 2020 07:57:45: 9000000 INFO @ Tue, 16 Jun 2020 07:57:47: 20000000 INFO @ Tue, 16 Jun 2020 07:57:48: 15000000 INFO @ Tue, 16 Jun 2020 07:57:51: 10000000 INFO @ Tue, 16 Jun 2020 07:57:52: 21000000 INFO @ Tue, 16 Jun 2020 07:57:54: 16000000 INFO @ Tue, 16 Jun 2020 07:57:57: 11000000 INFO @ Tue, 16 Jun 2020 07:57:58: 22000000 INFO @ Tue, 16 Jun 2020 07:58:00: 17000000 INFO @ Tue, 16 Jun 2020 07:58:02: 12000000 INFO @ Tue, 16 Jun 2020 07:58:04: 23000000 INFO @ Tue, 16 Jun 2020 07:58:05: 18000000 INFO @ Tue, 16 Jun 2020 07:58:09: 13000000 INFO @ Tue, 16 Jun 2020 07:58:10: 24000000 INFO @ Tue, 16 Jun 2020 07:58:11: 19000000 INFO @ Tue, 16 Jun 2020 07:58:15: 14000000 INFO @ Tue, 16 Jun 2020 07:58:16: 25000000 INFO @ Tue, 16 Jun 2020 07:58:17: 20000000 INFO @ Tue, 16 Jun 2020 07:58:21: 15000000 INFO @ Tue, 16 Jun 2020 07:58:22: 26000000 INFO @ Tue, 16 Jun 2020 07:58:23: 21000000 INFO @ Tue, 16 Jun 2020 07:58:27: 16000000 INFO @ Tue, 16 Jun 2020 07:58:28: 27000000 INFO @ Tue, 16 Jun 2020 07:58:29: 22000000 INFO @ Tue, 16 Jun 2020 07:58:33: 17000000 INFO @ Tue, 16 Jun 2020 07:58:34: 28000000 INFO @ Tue, 16 Jun 2020 07:58:35: 23000000 INFO @ Tue, 16 Jun 2020 07:58:39: 18000000 INFO @ Tue, 16 Jun 2020 07:58:40: 29000000 INFO @ Tue, 16 Jun 2020 07:58:41: 24000000 INFO @ Tue, 16 Jun 2020 07:58:44: #1 tag size is determined as 36 bps INFO @ Tue, 16 Jun 2020 07:58:44: #1 tag size = 36 INFO @ Tue, 16 Jun 2020 07:58:44: #1 total tags in treatment: 29533313 INFO @ Tue, 16 Jun 2020 07:58:44: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:58:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:58:44: #1 tags after filtering in treatment: 29533313 INFO @ Tue, 16 Jun 2020 07:58:44: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:58:44: #1 finished! INFO @ Tue, 16 Jun 2020 07:58:44: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:58:44: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:58:45: 19000000 INFO @ Tue, 16 Jun 2020 07:58:46: #2 number of paired peaks: 71 WARNING @ Tue, 16 Jun 2020 07:58:46: Too few paired peaks (71) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 07:58:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX105313/SRX105313.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX105313/SRX105313.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX105313/SRX105313.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX105313/SRX105313.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 07:58:47: 25000000 INFO @ Tue, 16 Jun 2020 07:58:51: 20000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 07:58:53: 26000000 INFO @ Tue, 16 Jun 2020 07:58:57: 21000000 INFO @ Tue, 16 Jun 2020 07:58:59: 27000000 INFO @ Tue, 16 Jun 2020 07:59:03: 22000000 INFO @ Tue, 16 Jun 2020 07:59:05: 28000000 INFO @ Tue, 16 Jun 2020 07:59:09: 23000000 INFO @ Tue, 16 Jun 2020 07:59:10: 29000000 INFO @ Tue, 16 Jun 2020 07:59:14: #1 tag size is determined as 36 bps INFO @ Tue, 16 Jun 2020 07:59:14: #1 tag size = 36 INFO @ Tue, 16 Jun 2020 07:59:14: #1 total tags in treatment: 29533313 INFO @ Tue, 16 Jun 2020 07:59:14: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:59:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:59:14: #1 tags after filtering in treatment: 29533313 INFO @ Tue, 16 Jun 2020 07:59:14: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:59:14: #1 finished! INFO @ Tue, 16 Jun 2020 07:59:14: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:59:14: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:59:14: 24000000 INFO @ Tue, 16 Jun 2020 07:59:16: #2 number of paired peaks: 71 WARNING @ Tue, 16 Jun 2020 07:59:16: Too few paired peaks (71) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 07:59:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX105313/SRX105313.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX105313/SRX105313.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX105313/SRX105313.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX105313/SRX105313.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 07:59:19: 25000000 INFO @ Tue, 16 Jun 2020 07:59:25: 26000000 INFO @ Tue, 16 Jun 2020 07:59:30: 27000000 INFO @ Tue, 16 Jun 2020 07:59:35: 28000000 INFO @ Tue, 16 Jun 2020 07:59:40: 29000000 INFO @ Tue, 16 Jun 2020 07:59:43: #1 tag size is determined as 36 bps INFO @ Tue, 16 Jun 2020 07:59:43: #1 tag size = 36 INFO @ Tue, 16 Jun 2020 07:59:43: #1 total tags in treatment: 29533313 INFO @ Tue, 16 Jun 2020 07:59:43: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:59:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:59:43: #1 tags after filtering in treatment: 29533313 INFO @ Tue, 16 Jun 2020 07:59:43: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:59:43: #1 finished! INFO @ Tue, 16 Jun 2020 07:59:43: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:59:43: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:59:45: #2 number of paired peaks: 71 WARNING @ Tue, 16 Jun 2020 07:59:45: Too few paired peaks (71) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 07:59:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX105313/SRX105313.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX105313/SRX105313.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX105313/SRX105313.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX105313/SRX105313.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。