Job ID = 6366161 SRX = SRX065686 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T22:48:07 prefetch.2.10.7: 1) Downloading 'SRR217392'... 2020-06-15T22:48:07 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T22:48:44 prefetch.2.10.7: HTTPS download succeed 2020-06-15T22:48:44 prefetch.2.10.7: 'SRR217392' is valid 2020-06-15T22:48:44 prefetch.2.10.7: 1) 'SRR217392' was downloaded successfully Read 7562402 spots for SRR217392/SRR217392.sra Written 7562402 spots for SRR217392/SRR217392.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:49 7562402 reads; of these: 7562402 (100.00%) were unpaired; of these: 3957814 (52.34%) aligned 0 times 2969497 (39.27%) aligned exactly 1 time 635091 (8.40%) aligned >1 times 47.66% overall alignment rate Time searching: 00:00:49 Overall time: 00:00:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 164159 / 3604588 = 0.0455 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:51:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX065686/SRX065686.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX065686/SRX065686.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX065686/SRX065686.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX065686/SRX065686.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:51:06: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:51:06: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:51:11: 1000000 INFO @ Tue, 16 Jun 2020 07:51:16: 2000000 INFO @ Tue, 16 Jun 2020 07:51:21: 3000000 INFO @ Tue, 16 Jun 2020 07:51:23: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:51:23: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:51:23: #1 total tags in treatment: 3440429 INFO @ Tue, 16 Jun 2020 07:51:23: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:51:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:51:24: #1 tags after filtering in treatment: 3440429 INFO @ Tue, 16 Jun 2020 07:51:24: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:51:24: #1 finished! INFO @ Tue, 16 Jun 2020 07:51:24: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:51:24: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:51:24: #2 number of paired peaks: 426 WARNING @ Tue, 16 Jun 2020 07:51:24: Fewer paired peaks (426) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 426 pairs to build model! INFO @ Tue, 16 Jun 2020 07:51:24: start model_add_line... INFO @ Tue, 16 Jun 2020 07:51:24: start X-correlation... INFO @ Tue, 16 Jun 2020 07:51:24: end of X-cor INFO @ Tue, 16 Jun 2020 07:51:24: #2 finished! INFO @ Tue, 16 Jun 2020 07:51:24: #2 predicted fragment length is 34 bps INFO @ Tue, 16 Jun 2020 07:51:24: #2 alternative fragment length(s) may be 4,34,595 bps INFO @ Tue, 16 Jun 2020 07:51:24: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX065686/SRX065686.05_model.r WARNING @ Tue, 16 Jun 2020 07:51:24: #2 Since the d (34) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 07:51:24: #2 You may need to consider one of the other alternative d(s): 4,34,595 WARNING @ Tue, 16 Jun 2020 07:51:24: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 07:51:24: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:51:24: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:51:31: #3 Call peaks for each chromosome... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:51:35: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX065686/SRX065686.05_peaks.xls INFO @ Tue, 16 Jun 2020 07:51:35: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX065686/SRX065686.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:51:35: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX065686/SRX065686.05_summits.bed INFO @ Tue, 16 Jun 2020 07:51:35: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (460 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 07:51:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX065686/SRX065686.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX065686/SRX065686.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX065686/SRX065686.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX065686/SRX065686.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:51:36: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:51:36: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:51:42: 1000000 INFO @ Tue, 16 Jun 2020 07:51:47: 2000000 INFO @ Tue, 16 Jun 2020 07:51:52: 3000000 INFO @ Tue, 16 Jun 2020 07:51:55: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:51:55: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:51:55: #1 total tags in treatment: 3440429 INFO @ Tue, 16 Jun 2020 07:51:55: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:51:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:51:55: #1 tags after filtering in treatment: 3440429 INFO @ Tue, 16 Jun 2020 07:51:55: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:51:55: #1 finished! INFO @ Tue, 16 Jun 2020 07:51:55: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:51:55: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:51:55: #2 number of paired peaks: 426 WARNING @ Tue, 16 Jun 2020 07:51:55: Fewer paired peaks (426) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 426 pairs to build model! INFO @ Tue, 16 Jun 2020 07:51:55: start model_add_line... INFO @ Tue, 16 Jun 2020 07:51:55: start X-correlation... INFO @ Tue, 16 Jun 2020 07:51:55: end of X-cor INFO @ Tue, 16 Jun 2020 07:51:55: #2 finished! INFO @ Tue, 16 Jun 2020 07:51:55: #2 predicted fragment length is 34 bps INFO @ Tue, 16 Jun 2020 07:51:55: #2 alternative fragment length(s) may be 4,34,595 bps INFO @ Tue, 16 Jun 2020 07:51:55: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX065686/SRX065686.10_model.r WARNING @ Tue, 16 Jun 2020 07:51:55: #2 Since the d (34) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 07:51:55: #2 You may need to consider one of the other alternative d(s): 4,34,595 WARNING @ Tue, 16 Jun 2020 07:51:55: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 07:51:55: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:51:55: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:52:02: #3 Call peaks for each chromosome... BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:52:06: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX065686/SRX065686.10_peaks.xls INFO @ Tue, 16 Jun 2020 07:52:06: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX065686/SRX065686.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:52:06: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX065686/SRX065686.10_summits.bed INFO @ Tue, 16 Jun 2020 07:52:06: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (197 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 07:52:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX065686/SRX065686.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX065686/SRX065686.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX065686/SRX065686.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX065686/SRX065686.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:52:06: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:52:06: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:52:11: 1000000 INFO @ Tue, 16 Jun 2020 07:52:16: 2000000 INFO @ Tue, 16 Jun 2020 07:52:21: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 07:52:23: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:52:23: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:52:23: #1 total tags in treatment: 3440429 INFO @ Tue, 16 Jun 2020 07:52:23: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:52:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:52:23: #1 tags after filtering in treatment: 3440429 INFO @ Tue, 16 Jun 2020 07:52:23: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:52:23: #1 finished! INFO @ Tue, 16 Jun 2020 07:52:23: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:52:23: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:52:23: #2 number of paired peaks: 426 WARNING @ Tue, 16 Jun 2020 07:52:23: Fewer paired peaks (426) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 426 pairs to build model! INFO @ Tue, 16 Jun 2020 07:52:23: start model_add_line... INFO @ Tue, 16 Jun 2020 07:52:23: start X-correlation... INFO @ Tue, 16 Jun 2020 07:52:23: end of X-cor INFO @ Tue, 16 Jun 2020 07:52:23: #2 finished! INFO @ Tue, 16 Jun 2020 07:52:23: #2 predicted fragment length is 34 bps INFO @ Tue, 16 Jun 2020 07:52:23: #2 alternative fragment length(s) may be 4,34,595 bps INFO @ Tue, 16 Jun 2020 07:52:23: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX065686/SRX065686.20_model.r WARNING @ Tue, 16 Jun 2020 07:52:23: #2 Since the d (34) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 07:52:23: #2 You may need to consider one of the other alternative d(s): 4,34,595 WARNING @ Tue, 16 Jun 2020 07:52:23: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 07:52:23: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:52:23: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:52:31: #3 Call peaks for each chromosome... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 07:52:34: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX065686/SRX065686.20_peaks.xls INFO @ Tue, 16 Jun 2020 07:52:34: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX065686/SRX065686.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:52:34: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX065686/SRX065686.20_summits.bed INFO @ Tue, 16 Jun 2020 07:52:34: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (55 records, 4 fields): 2 millis CompletedMACS2peakCalling