Job ID = 6366156 SRX = SRX065681 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T22:34:21 prefetch.2.10.7: 1) Downloading 'SRR217387'... 2020-06-15T22:34:21 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T22:34:53 prefetch.2.10.7: HTTPS download succeed 2020-06-15T22:34:53 prefetch.2.10.7: 'SRR217387' is valid 2020-06-15T22:34:53 prefetch.2.10.7: 1) 'SRR217387' was downloaded successfully Read 7350402 spots for SRR217387/SRR217387.sra Written 7350402 spots for SRR217387/SRR217387.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:05 7350402 reads; of these: 7350402 (100.00%) were unpaired; of these: 1440839 (19.60%) aligned 0 times 5065564 (68.92%) aligned exactly 1 time 843999 (11.48%) aligned >1 times 80.40% overall alignment rate Time searching: 00:01:05 Overall time: 00:01:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 762979 / 5909563 = 0.1291 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:37:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX065681/SRX065681.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX065681/SRX065681.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX065681/SRX065681.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX065681/SRX065681.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:37:50: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:37:50: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:37:54: 1000000 INFO @ Tue, 16 Jun 2020 07:37:58: 2000000 INFO @ Tue, 16 Jun 2020 07:38:02: 3000000 INFO @ Tue, 16 Jun 2020 07:38:07: 4000000 INFO @ Tue, 16 Jun 2020 07:38:11: 5000000 INFO @ Tue, 16 Jun 2020 07:38:12: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:38:12: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:38:12: #1 total tags in treatment: 5146584 INFO @ Tue, 16 Jun 2020 07:38:12: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:38:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:38:12: #1 tags after filtering in treatment: 5146584 INFO @ Tue, 16 Jun 2020 07:38:12: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:38:12: #1 finished! INFO @ Tue, 16 Jun 2020 07:38:12: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:38:12: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:38:12: #2 number of paired peaks: 402 WARNING @ Tue, 16 Jun 2020 07:38:12: Fewer paired peaks (402) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 402 pairs to build model! INFO @ Tue, 16 Jun 2020 07:38:12: start model_add_line... INFO @ Tue, 16 Jun 2020 07:38:12: start X-correlation... INFO @ Tue, 16 Jun 2020 07:38:12: end of X-cor INFO @ Tue, 16 Jun 2020 07:38:12: #2 finished! INFO @ Tue, 16 Jun 2020 07:38:12: #2 predicted fragment length is 36 bps INFO @ Tue, 16 Jun 2020 07:38:12: #2 alternative fragment length(s) may be 2,36,98,557,569 bps INFO @ Tue, 16 Jun 2020 07:38:12: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX065681/SRX065681.05_model.r WARNING @ Tue, 16 Jun 2020 07:38:12: #2 Since the d (36) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 07:38:12: #2 You may need to consider one of the other alternative d(s): 2,36,98,557,569 WARNING @ Tue, 16 Jun 2020 07:38:12: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 07:38:12: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:38:12: #3 Pre-compute pvalue-qvalue table... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:38:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX065681/SRX065681.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX065681/SRX065681.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX065681/SRX065681.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX065681/SRX065681.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:38:20: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:38:20: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:38:23: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:38:24: 1000000 INFO @ Tue, 16 Jun 2020 07:38:28: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX065681/SRX065681.05_peaks.xls INFO @ Tue, 16 Jun 2020 07:38:28: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX065681/SRX065681.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:38:28: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX065681/SRX065681.05_summits.bed INFO @ Tue, 16 Jun 2020 07:38:28: Done! INFO @ Tue, 16 Jun 2020 07:38:28: 2000000 pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (574 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 07:38:33: 3000000 INFO @ Tue, 16 Jun 2020 07:38:37: 4000000 INFO @ Tue, 16 Jun 2020 07:38:41: 5000000 INFO @ Tue, 16 Jun 2020 07:38:42: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:38:42: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:38:42: #1 total tags in treatment: 5146584 INFO @ Tue, 16 Jun 2020 07:38:42: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:38:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:38:42: #1 tags after filtering in treatment: 5146584 INFO @ Tue, 16 Jun 2020 07:38:42: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:38:42: #1 finished! INFO @ Tue, 16 Jun 2020 07:38:42: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:38:42: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:38:42: #2 number of paired peaks: 402 WARNING @ Tue, 16 Jun 2020 07:38:42: Fewer paired peaks (402) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 402 pairs to build model! INFO @ Tue, 16 Jun 2020 07:38:42: start model_add_line... INFO @ Tue, 16 Jun 2020 07:38:42: start X-correlation... INFO @ Tue, 16 Jun 2020 07:38:42: end of X-cor INFO @ Tue, 16 Jun 2020 07:38:42: #2 finished! INFO @ Tue, 16 Jun 2020 07:38:42: #2 predicted fragment length is 36 bps INFO @ Tue, 16 Jun 2020 07:38:42: #2 alternative fragment length(s) may be 2,36,98,557,569 bps INFO @ Tue, 16 Jun 2020 07:38:42: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX065681/SRX065681.10_model.r WARNING @ Tue, 16 Jun 2020 07:38:42: #2 Since the d (36) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 07:38:42: #2 You may need to consider one of the other alternative d(s): 2,36,98,557,569 WARNING @ Tue, 16 Jun 2020 07:38:42: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 07:38:42: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:38:42: #3 Pre-compute pvalue-qvalue table... BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:38:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX065681/SRX065681.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX065681/SRX065681.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX065681/SRX065681.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX065681/SRX065681.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:38:50: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:38:50: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:38:53: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:38:54: 1000000 INFO @ Tue, 16 Jun 2020 07:38:58: 2000000 INFO @ Tue, 16 Jun 2020 07:38:59: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX065681/SRX065681.10_peaks.xls INFO @ Tue, 16 Jun 2020 07:38:59: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX065681/SRX065681.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:38:59: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX065681/SRX065681.10_summits.bed INFO @ Tue, 16 Jun 2020 07:38:59: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (188 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 07:39:03: 3000000 INFO @ Tue, 16 Jun 2020 07:39:07: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 07:39:12: 5000000 INFO @ Tue, 16 Jun 2020 07:39:12: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:39:12: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:39:12: #1 total tags in treatment: 5146584 INFO @ Tue, 16 Jun 2020 07:39:12: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:39:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:39:12: #1 tags after filtering in treatment: 5146584 INFO @ Tue, 16 Jun 2020 07:39:12: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:39:12: #1 finished! INFO @ Tue, 16 Jun 2020 07:39:12: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:39:12: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:39:13: #2 number of paired peaks: 402 WARNING @ Tue, 16 Jun 2020 07:39:13: Fewer paired peaks (402) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 402 pairs to build model! INFO @ Tue, 16 Jun 2020 07:39:13: start model_add_line... INFO @ Tue, 16 Jun 2020 07:39:13: start X-correlation... INFO @ Tue, 16 Jun 2020 07:39:13: end of X-cor INFO @ Tue, 16 Jun 2020 07:39:13: #2 finished! INFO @ Tue, 16 Jun 2020 07:39:13: #2 predicted fragment length is 36 bps INFO @ Tue, 16 Jun 2020 07:39:13: #2 alternative fragment length(s) may be 2,36,98,557,569 bps INFO @ Tue, 16 Jun 2020 07:39:13: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX065681/SRX065681.20_model.r WARNING @ Tue, 16 Jun 2020 07:39:13: #2 Since the d (36) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 07:39:13: #2 You may need to consider one of the other alternative d(s): 2,36,98,557,569 WARNING @ Tue, 16 Jun 2020 07:39:13: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 07:39:13: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:39:13: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 07:39:23: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:39:29: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX065681/SRX065681.20_peaks.xls INFO @ Tue, 16 Jun 2020 07:39:29: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX065681/SRX065681.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:39:29: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX065681/SRX065681.20_summits.bed INFO @ Tue, 16 Jun 2020 07:39:29: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (29 records, 4 fields): 1 millis CompletedMACS2peakCalling